Aim: The majority of medicines used in Poland are generic drugs – substitutions of the original. Objective of the study was to obtain information on the current knowledge about generic medicines among Polish patients with epilepsy. Material and Methods: The study was conducted based on a self-developed questionnaire. The questionnaire consisted of 25 questions, including questions regarding their knowledge about generics, their previous experience and the factors behind these choices. Results: Overall, 1220 questionnaires were analyzed. Among all patients, 66.39% reportedly heard about generics. Of these 61.48% of patients had used the generic drugs in past. A significant proportion of participants had never been recommended to switch to a generic medicine by their health care professional (22.95% physician and 13.93% pharmacist). Statistically relevant differences were observed regarding the respondents’ kind and level of education, place of residence, and net income per household. Conclusions: Significant gaps were identified in the knowledge and perceptions among patients regarding generic medicines especially in relation to efficacy and safety of generic medicines. Efforts must be directed towards increasing public awareness of generic medicines and should also focus on educating patients about generic medicines.

The dependence of the width of annual rings of woody plants on the characteristics of the snow cover is analyzed in various natural zones of the West Siberian Plain and adjacent mountainous areas: the maximum thickness and water reserve for the entire winter period and for individual months, the dates of disappearing, establishment, and duration of the occurrence of a stable snow cover. It has been shown that the role of the thickness and water content of snow cover for the radial growth of trees is differentiated by geographical location. On the plain, it intensifies in the forest-tundra and dry steppe. The response of radial growth to snow cover in the upper and lower parts of the forest belt is often opposite. Dates of establishment of stable snow cover are more important for tree growth compared to dates of disappearance. Dates of disappearance of stable snow cover are more significant in the southern regions than in the northern ones. The value of the duration of the period with stable snow cover for tree growth is higher in the southern regions.

Post-industrial recycling (PIR) and post-consumer recycling (PCR) are measures to sustain re-sources by improving materials’ circularity and sustainability. Currently, circularity is mostly measured as the degree of reutilization of a material from 0 to 100 % at product or company lev-el. This lacks in assessing the resources’ usage over multiple product life cycles. Therefore, we propose to assess circularity as (i) the frequency resources are used in products (effective circu-larity eC) and (ii) a vehicle to reduce virgin resource use and environmental impacts (environ-mentally efficient circularity eeC). Besides, to compare the environmental impacts of using recy-cled materials from PIR or PCR, we analyse their impact reduction potential (IRP) indicating the environmental benefits of recycling in relation to virgin material put onto the market. We demonstrate the suggested indicators for a case study material: polypropylene. For this polymer type, the eC ranges between 0.93 and 9.08 uses of the resource on average depending on collec-tion, sorting, and recycling rates. Likewise, the eeC ranges between 0.31 and 1.50 uses per kg of CO2 equivalents emitted. PCR has a higher IRP regarding climate change impacts than PIR in all analysed scenarios. The results reveal the relevance of PCR and PIR beyond the product life cycle.

Number of studies claim that tobacco control (TC) regulations are associated with reductions in smoking-related hospitalisation rates, however very few have estimated the impact of TC laws (TCL) at both countrywide and regional levels, and none of them - in relation to compliance with TC regulations. This study evaluates the effects of Russian TCL on hospital admission (HA) rates for pneumonia countrywide and in 10 Russian regions and the extent of these effects in connection with the compliance to TCL. Methods: HA rates for pneumonia during 2005–2019 were analysed to compare the periods before and after the adoption of TCL in 2013. An interrupted time series design and a Poisson regression model were used to estimate the immediate and long-term effects of TCL on pneumonia annual hospitalisation rates after the TCL adoption, compared with the pre-law period. The 10 Russian regions were compared using the TCL Implementation Scale (TCIS) developed on the basis of the results of Russian TC policy evaluation survey; Spearman’s rank correlation and linear regression models were employed. Results showed a 14.3% reduction in HA rates for pneumonia (RR 0.88; p=0.01) after the adoption of TCL in Russia with significant long-term effect after 2013 (RR 0.86; p=0.006). Regions with better enforcement of TCL exhibited greater reductions in pneumonia HA rates (rsp=−0.55; p=0.04); (=−4.21; p=0.02). Conclusions: TCL resulted in a sustained reduction in pneumonia hospitalisation rates, but these effects, varying by region, may depend on the scale of the TCL enforcement.

The investment in digital e-Health services is a priority direction in the development of global health care systems. While people are increasingly using the Web for health information, it is not entirely clear what is the physicians’ attitude towards digital transformation, and the acceptance of new technologies in healthcare. The aim of this cross-sectional survey study was to investigate physicians’ self-digital skills, and their opinions on obtaining online health knowledge by patients, as well as the recognition of physicians’ attitudes towards e-Health solutions. Principal Component Analysis (PCA) was performed to emerge the variables from self-designed questionnaire, and cross-sectional analysis comparing descriptive statistics and correlations for dependent variables using the one-way ANOVA (F-test). 307 physicians participated in the study, reported using the internet mainly several times a day (66.8%). Most participants (70.4%) were familiar with new technologies and rate their e-Health literacy high, although 84.0% reported the need for additional training in this field, and reported a need to introduce a larger number of subjects shaping digital skills (75.9%) in medical studies 53.4% of physicians perceived Internet-sourced information as sometimes reliable, and in general assessed the effects of using it by their patients negatively (41.7%). Digital skills increased significantly with frequency of internet use (F = 13.167; p = 0.0001), and decreased with physicians’ age, and the need for training. Those who claimed that patients often experienced health benefits from online health showed higher digital skills (-1.06). Physicians most often recommended their patients to obtain laboratory test results online (32.2%), and to arrange medical appointments via the Internet (27.0%). Along with the deterioration of physicians’ digital skills, the recommendation of e-Health solutions decreased (r = 0.413), and lower the assessment of e-Health solutions for the patient (r = 0.449). Physicians perceive digitization as a sign of the times, and frequently use its tools in daily practice. The evaluation of Dr. Google’s phenomenon and online health is directly related to their own e-Health literacy skills, but there is still a need for practical training to deal with digital revolution.

Lack of sleep is a factor that disrupts the receptors' reception of information from the environ-ment and contributes to the emergence of problems with maintaining balance. The main pur-pose of this study was to find out whether Computer Dynamic Posturography could be a useful tool in distinguishing between people who sleep well from people with insomnia. The study participants were 76 male students who were divided into groups based on the results obtained from the Pittsburgh Sleep Quality Questionnaire. In each group, postural stability had been tested using three main tests: Strategy Organization Test SOT, Motor Control Test MCT and Ad-aptation Test ADT. The results of the analysis show that the obtained results differ in the exam-ined groups under the SOT test. Among people with insomnia, higher values of the tested pa-rameters were noted, than with people who sleep well, which translates into a worse ability to maintain balance. The greatest impact is observed when using eyesight and a vestibular system to maintain a stable posture. It was confirmed that Computer Dynamic Posturography is used to differentiate between people who sleep well from people with insomnia in the group of men. Lack of sleep significantly disturbs postural stability.

One of the critical tools for early detection and subsequent evaluation of the incidence of lung diseases is chest radiography. This study presents a real-world implementation of a convolutional neural network (CNN) based Carebot Covid app to detect COVID-19 from chest X-ray (CXR) images. Our proposed model takes the form of a simple and intuitive application. Used CNN can be deployed as a STOW-RS prediction endpoint for direct implementation into DICOM viewers. The results of this study show that the deep learning model based on DenseNet and ResNet architecture can detect SARS-CoV-2 from CXR images with precision of 0.981, recall of 0.962 and AP of 0.993.

We provide a biochronology of Jurassic planktonic foramininfera, using first order linkage to ammonite and nannofossil stratigraphy and geochronology. This enigmatic and understudied group of microfossils occurred from middle Toarcian through Tithonian time, from ~180 to ~143 Ma; its origin is unknown. There are three genera: Globuligerina, Conoglobigerina and Petaloglobigerina. The genus Globuligerina, with a smooth to pustulose test surface texture appeared in Toarcian (late Early Jurassic) and Conoglobigerina, with a rough reticulate test surface texture in Oxfordian (early Late Jurassic) time. The genus Petaloglobigerina, with a petaloid last whorl and one or more twisted and claviform chambers evolved in early Kimmeridgian time from Globuligerina balakhmatovae. We recognize stratigraphic events from eleven species across four evolutionary lineages, calibrated to Geologic Time Scale 2020. A dramatic faunal change over, which is not well documented led to the survival of only one taxon, most likely Gobuligerina oxfordiana in the Tithonian. During the Berriasian several new taxa appeared.

We aimed to investigate the ability of children aged 5–14 years old (preschoolers, primary schoolers, and preteens) to assess and anticipate time intervals. 287 Russian children aged 5–14 years old and 26 adults of control group participated in our study. The neuropsychological assessment, Wechsler Intelligence Scale for Children and a battery of time-related tests were applied. All groups of children overestimated the event’s duration, although the accuracy of the second estimations increased among the participants aged 6–8 years after a prompt was offered. A zone of proximal development for time anticipation task was detected for children aged 9-11 years, when the prompt could significantly improve the accuracy of time perception. The participants overestimated the duration of both upcoming and past events, with the degree of overestimation being found to be negatively correlated with age. Further, a higher degree of accuracy in terms of time estimation was found to be correlated with higher scores on the attention and memory tests, and accuracy of time anticipation was associated with scores of praxis test.

Udder diseases (mastitis) are a serious cause of economic losses in sheep breeding as they have a negative impact on lamb rearing and the quality of dairy products. So far the progress in treatment and prevention of these diseases has been insufficient, giving ground for searching possibilities of using natural immunity to combat mastitis. The aim of the study was to assess the relationship between the microsatellite polymorphism of selected Ovar-MHC genes and the health status of the mammary gland of sheep. The research was carried out on sheep of the Polish Heath and Polish Lowland breeds. In ovine milk the number of somatic cells (SCC) and the percentage of the lymphocyte subpopulation were assessed. On the basis of genomic DNA, molecular analysis of the Ovar-MHC gene fragments (OLADRB1, OLADRB2, OMHC1) polymorphism was performed. Significant differences were found in SCC and the percentage of lymphocytes (CD4, CD8, CD19) in the milk depending on the alleles of the Ovar-MHC genes. Alleles of 488 bp (DRB1) and 284 bp (DRB2) were found more frequently in sheep with healthy udders, while carriers of the 508 bp (DRB1) and 272 bp (DRB2) alleles were more prone to subclinical mastitis. The obtained results justify the need for further research in order to better understanding the genetic basis of mastistis and to search for effective molecular markers that can be used in breeding practice.

Watching the international COVID-19 epidemics unfold during February and early March 2020, we began to highlight how outbreaks in care homes were inevitable, given the vulnerable patients in close proximity, and pressures on social care to help relieve pressure on the NHS. We suggested public health measures would significantly impact on care homes. Specifically we felt all homes would have multiple individuals isolated within days of introduction of guidance requiring isolation of new cough (or fever), and that a clear definition of an outbreak would be needed that differentiated COVID19 from influenza. We share the experiences of a GP practice looking after 900 nursing or dual registration care homes in the London Borough of Ealing in the early stages of the COVID19 Pandemic 2020. We believe that the altered presentation of cases of COVID19 in care homes contributed to the size of outbreaks, and that keeping COVID19 out of homes is the only way to manage this disease, with early isolation and complete segregation of positive and negative cases. We have seen over 300 suspected cases resulting in four fold (n=175) average monthly death rates, three fold usual issue of anticipatory medications, and approximately 32% mortality rate (up to 43% in over 90’s). Discharge pathways from hospital and admissions to care homes must be clear and robust. COVID19 naïve patients should not be admitted to units with outbreaks and COVID19 positive patients should not be admitted to anything other than a designated “hot” home until risk of contagion is passed. Some patients are testing positive at over 30 days since initial mild symptoms.

This paper investigates a range of popular differential evolution (DE) configurations to identify components responsible for emergence of structural bias – a recently identified tendency of algorithms to prefer some regions of search space over others, for reasons unrelated to objective function values. Previous work has explored this tendency for genetic algorithms (GA) and particle swarm optimisation (PSO), finding a relationship between population size and extent of structural bias, hence highlighting potential weaknesses of those algorithms. In current article, we focus on DE, extend the investigation to include consideration of an algorithmic component that is often overlooked – constraint handling mechanism. Towards this end, a wide range of DE configurations was tested here. Results suggest that DE is generally robust to structural bias. Unlike the case with GA and PSO, population size seems to have no influence on DE structural bias. Only one of variants studied – DE/current-to-best/1/bin – shows clear signs of bias, however, we show that this effect is mitigated by a judicious choice of constraint handling technique. These findings contribute towards explaining widespread success of DE variants in algorithm comparison studies; its robustness to structural bias represents the absence of a factor that may confound other algorithms.

Background: Infectious disease is a major challenge in aquaculture and poses a constraint for development of farming of new species. In 2017, Siberian sturgeon (Acipenser baerii) juveniles were imported from Italy to a Swedish farm. Due to stressful conditions, 30% died during transport and in the first days after arrival. Ten days after arrival, mortalities started to occur again. Within two months, only 5% of the juveniles were still alive. Methods: Diseased fish were transported live to the National Veterinary Institute (SVA) for necropsy and further analysis. Pathological and histopathological investigation was conducted. Virology was performed on gills and internal organs by cell culture isolation and specific PCR protocols. Results: The juveniles displayed neurological signs such as lethargy, inability to maintain upright position and erratic swimming. Body condition was low. Gills were pale. One fish had petechial hemorrhage on the abdomen and the snout. The ventricles were air-filled with, but swim bladders were deflated. One specimen had intestinal hemorrhage. Viral cell cultures were negative, but PCR of gills and internal organs detected the presence of Acipenser Iridovirus European (AcIV-E). Conclusions: AcIV-E was associated with disease and high mortality in the sturgeon juveniles. Stress probably aggravated the course of the infection.

The DNA Damage Response (DDR) is a complex signaling network that comes into play when cells experience genotoxic stress. Upon DNA damage, cellular signaling pathways are rewired to slow down cell cycle progression and allow recovery. However, when the damage is beyond repair, cells activate complex and still not fully understood mechanisms, leading to a complete proliferative arrest or cell death. A number of conventional and novel anti-neoplastic treatments rely on causing DNA damage or on the inhibition of the DDR in cancer cells. However, the identification of molecular determinants directing cancer cells toward recovery or death upon DNA damage is still far from complete, and it is object of intense investigation. SPRY-COntaining RAN binding Proteins (Scorpins) RANBP9 and RANBP10 are evolutionarily conserved and ubiquitously expressed proteins whose biological functions are still debated. RANBP9 has been previously implicated in cell proliferation, survival, apoptosis and migration. Recent studies also showed that RANBP9 is involved in the Ataxia Telangiectasia Mutated (ATM) signaling upon DNA damage. Accordingly, cells lacking RANBP9 show increased sensitivity to genotoxic treatment. Although there is no published evidence, extensive protein similarities suggest that RANBP10 might have partially overlapping functions with RANBP9. Like RANBP9, RANBP10 bears sites putative target of PIK-kinases and high throughput studies found RANBP10 to be phosphorylated following genotoxic stress. Therefore, this second Scorpin might be another overlooked player of the DDR alone or in combination with RANBP9. This review focuses on the relatively unknown role played by RANBP9 and RANBP10 in responding to genotoxic stress.

The classification of Trojans in modern analog microcircuits is considered. Using high-speed operational amplifiers (Op-Amps) as an example, the relationship between Op-Amp circuit design and the formation of undesirable states in typical switching circuits is shown. Circuit solutions are proposed to minimize the Trojan states of high-speed Op-Amps. These include limiting the maximum output currents of the input stages of the Op-Amp during the transient process and the use of differentiating transient correction circuits in the Op-Amp.

Urolithiasis is an increasingly common clinical problem worldwide. The formation of stones is a combination of metabolic status, environmental factors, family history and many other aspects. It’s important to find new ways to quickly detect and assess urolithiasis because it causes sudden, severe pain and often comes back. One way to do this is by exploring new biomarkers. Current advances in proteomic studies provide a great opportunity for breakthroughs in this field. The study focuses on protein biomarkers and their connection to kidney damage and inflammation during urolithiasis.

This study was conducted to investigate bit use in equestrian sports, focusing on welfare and performance as bit use has been subject of debate. The multi-faceted study combines a literature review, survey, and video analyses. Research on physiological and behavioral effects of bit use, discussing concerns and benefits, was evaluated. The survey involved 250 equestrians, gathering data on experiences, opinions, and practices regarding bit usage. Results demonstrate that different bits are used during competitions and that riders prioritize factors like horse satisfaction, consistent connection, and chewing behavior when riding at home, while show use focusses on factors like control. Video analyses showed high conformity between veterinary expert and show jumper, indicating reliability and validity. Pelham and loose ring bits were most used, while 3-ring and full cheek bits were associated with the most aversive movements, particularly opening of the mouth. Other defense movements included putting ears back, tail swishing, and head tilting. The study highlighted the need for understanding bit preferences in various settings and cautioned against generalizing bit usage based solely on show observations. It also emphasized the potential for developing new designs that prioritize horse comfort and responsiveness, to enhance communication and partnership between horses and riders.

Lycopene is a compound of colored origin that shows strong antioxidant activity. The ability to neutralize free radicals by lycopene is the basis of its pro-health effect, including the ability to inhibit the development of many civilization diseases. Therefore, this study focuses on the importance of the antioxidant effect of lycopene in inhibiting the development of such diseases as cardiovascular diseases, diseases of the etiology of etiology, diseases within the nervous system.

Diabetes mellitus is one of the most common metabolic diseases worldwide, and its long-term complications include neuropathy, referring both to the peripheral and to the central nervous system. Detrimental effects of dysglycemia, especially hyperglycemia, on the structure and function of blood-brain barrier (BBB) seem to be a significant backgrounds of diabetic neuropathy pertaining to the central nervous system (CNS). Effects of hyperglycemia, including excessive glucose influx to insulin independent cells, may induce oxidative stress and secondary innate immunity dependent inflammatory response which can damage cells within the CNS, thus promoting neurodegeneration and dementia. Advanced glycation end products (AGE) may exert similar, pro-inflammatory effects through activating receptors for advanced glycation end products (RAGE), as well as some pattern-recognition receptors (PRR). Moreover, long-term hyperglycemia can promote brain insulin resistance, which may in turn promote Aβ aggregate accumulation and tau hyperphosphorylation. This review is focused on a detailed analysis of the effects mentioned above towards the CNS, with special regard to mechanisms taking part in the pathogenesis of central long-term complications of diabetes mellitus initiated by the loss of BBB integrity.

The genus Allium L. attracts the attention of researchers due to the presence of valuable medicinal, nutritional and decorative properties, adaptive capabilities, resistance to pests and diseases, ecological plasticity in its representatives, which contributes to the competitiveness of species and the manifestation of a high degree of adaptation outside natural areas and wide geographical distribution. Allium resources in Russia are a potential source of genes for expanding the genetic base of agricultural crops. The leaves of Allium crops have a short freshness period and quickly deteriorate due to spoilage and loss of water. Spoilage and loss of water affect their freshness and properties. The aim of the work was to establish the yield and change in the quality of green leaves of Allium species introduced into the Moscow region during short-term storage, depending on the type of packaging, storage period and temperature. 4-5-year-old plants of 4 species (A. altyncolicum, A. chyatophorum, A. nutans and A. turkestanicum) were grown on the collection plot of perennial onions of VNIIO, a branch of the FGBNU FNTSO. In the conditions of the Moscow region, the yield per leaf generation varied from 2.7 kg/m2 (A. altyncolicum) to 4.9 kg/m2 (A. cyathophorum). The maximum yield of marketable products was noted in hermetically sealed plastic bags when stored for 10 days at a temperature of +6 ... + 80С in a refrigerator with controlled conditions: A. turke-stanicum - 70.0%, A. cyathophorum - 75.5%, A. altyncolicum - 84.9%, A. nutans - 92.9%. However, an increase in the dry matter content (10.1-15.5%) was found during storage in an open polymer box in all tested species. The maximum content of vitamin C during storage in hermetically sealed bags with a density of 100 μm was found in A. altyncolicum (37.1 mg%) and A. nutans (42 mg%). In other species, the maximum value of this indicator was noted before storage. A slight increase in the amount of sugars after storage for 10 days was noted in species with a linear leaf shape when stored in a polymer box. Temperature control is the most effective approach to extend the shelf life of fresh green leaves by measuring its weight loss and vitamin C. Packaging cannot prevent spoilage of vegetables but can protect it against contamination, damage and excessive moisture loss.

The successful vaccination implies the induction of effective specific immune responses. We intend to find biomarkers among various immune cell subpopulations, cytokines and antibodies which could be used to predict the levels of specific antibody- and cell-mediated responses after measles-mumps-rubella vaccination. We measured 59 baseline immune status parameters (frequencies of 42 immune cell subsets, levels of 13 cytokines, immunoglobulins) before vaccination and 13 response variables (specific IgA and IgG, antigen-induced IFN-γ production, CD107a expression on CD8+ T lymphocytes, and cellular proliferation levels by CFSE dilution) 6 weeks after vaccination for 19 individuals. Statistically significant Spearman correlations between some baseline parameters and response variables were found for each response variable (p<0.05). Due to the low number of observations relative to the number of baseline parameters and missing data for some observations, we utilized three feature selection strategies to select potential predictors of the post-vaccination responses among baseline variables: (a) screening of the variables based on the correlation analysis, (b) supervised screening based on the information of changes of baseline variables at day 7, (c) implicit feature selection is performed using regularization-based sparse regression. We identified optimal multivariate liner regression models for predicting the effectiveness of vaccination against measles-mumps-rubella using the baseline immune status parameters. It turned out that the sufficient number of predictor variables ranges from one to five depending on the response variable of interest.

Huntington’s Disease (HD) is a devastating neurodegenerative disorder caused by a CAG trinucleotide repeat expansion in the HTT gene, for which no disease modifying therapies are currently available. Much of the recent research has focused on developing therapies to directly lower HTT expression, and while promising, these therapies have presented several challenges regarding administration and efficacy. Another promising therapeutic approach is the modulation of HTT post-translational modifications (PTMs) that are dysregulated in disease and have shown to play a key role in HTT toxicity. Among all PTMs, modulation of HTT phosphorylation has been proposed as an attractive therapeutic option due to the possibility of orally administering specific kinase effectors. One of the kinases described to participate in HTT phosphorylation is Protein Kinase CK2. CK2 has recently emerged as a target for the treatment of several neurological and psychiatric disorders, although its role in HD remains controversial. While pharmacological studies in vitro inhibiting CK2 resulted in reduced HTT phosphorylation and increased toxicity, genetic approaches in mouse models of HD have provided beneficial effects. In this review we discuss potential therapeutic approaches related to the manipulation of HTT-PTMs with special emphasis on the role of CK2 as a therapeutic target in HD.

The authors of the article performed computer simulation of buffer amplifiers (BA), which have medium and extremely small values of the offset voltage's systematic component (V_off), for different technological processes (Si, GaAs and GaN). The proposed control units are distinguished by a small number of elements and allow operation in the range of low and high temperatures. The variants of circuitry implementation of control units based on GaAs, GaN depletion-mode CMOS and JFET technological processes are considered. The results of the comparative modeling showed that the basic circuit of the BA on two field-effect transistors, when implemented on various modifications of GaN MOS and depletion-mode MOS transistors, provides sufficiently low values of the offset voltage's systematic component (less than 2 μV). The proposed BAs are designed for use in the structure of the Sallen-Key low-pass filter (LPF) when they are implemented both on mid-frequency Si CJFET and on GaAs microwave transistors. Low values of the LPF V_off have a positive effect on the effective capacity of the ADC. An example of switching on a BA in the JFET OpAmp structure based on the depletion-mode MOS input stage and a “folded” cascode, which, with 100% negative feedback, can be used in the Sallen-Key LPF, is considered. Computer simulation of the JFET/MOS OpAmp showed that the OpAmp has an open-loop voltage gain of 76-85dB, and its V_off is within 7µV in the temperature range from -60°C to +120°C. The presented circuitry of buffer amplifiers is intended, first of all, for the tasks of designing precision Sallen-Key low-pass filter (low-pass filter, high-pass filter, PF, RF).

Protein immobilization for the functionalization of particles is used in various applications, including biosensors, lateral-flow immunoassays (LFIA), bead-based assays, and others. Common methods for the quantification of bound protein are measuring protein in the supernatant before and after coating and calculating the difference. This popular approach has the potential for a significant overestimation of the amount of immobilized protein since layers not directly bound to the surface (soft protein corona) are usually lost during washing and handling. Only the layer directly bound to the surface (hard corona) can be used in subsequent assays. A simplified amino acid analysis method based on acidic hydrolysis and RP-HPLC-FLD of tyrosine and phenylalanine (aromatic amino acid analysis, AAAA) is proposed to directly quantify protein bound to the surface of gold nano- and latex microparticles. The results are compared with indirect methods such as colorimetric protein assays, such as Bradford, bicinchoninic acid (BCA), as well as AAAA of the supernatant. For both particle types, these indirect quantification techniques show a protein overestimation of up to 1700% compared to the direct AAAA measurements. In addition, protein coating on latex particles was performed both passively through adsorption and covalently through EDC/sulfo-NHS chemistry. Our results showed no difference between the immobilization methodologies. This finding suggests that usual protein determination methods are no unambiguous proof of a covalent conjugation on particles or beads.

This work shows a correlation between light reflectance, absorption, and morphologies of series of (1-x) BM–x PT, (x = 0.0, 0.02, 0.04, 0.08, 0.12, 0.16, 0.24) ceramics composite. The (1-x) BM–x PT showed features of a black mirror with a low optical energy gap. The measured Vis-NIR diffused reflectance enabled the calculation of the energy gap using the modified Kubelka-Munk function. The estimated energy gap was lower than 1.5 eV related to low reflectance in the Vis-NIR range. Moreover, obtained histograms of grains, using scanning electron microscope, enabled the correlation between grains size and amount of lead titanate. We deduced from the ceramics surface morphology that marked porosity also induced reflectivity of low magnitude. We correlated the magnitude of the energy gap with phases of the BM-PT composite and with the electrical conductivity activation energy reported in the literature. Our results findings opened prospect studied materials for optical applications.

According to the World Health Organization 94% of global malaria cases and 94% of global malaria deaths have been reported from Africa. Unfortunately, it is difficult to determine the exact prevalence of disease in some African countries due to a large number of asymptomatic cases. The aim of this study was to assess the prevalence of malaria infections in seemingly healthy children living in the Central African Republic (CAR). CareStartTM Malaria HRP2 Pf Ag RDT targeting Plasmodium falciparum was used to test a group of 500 asymptomatic children aged 1-15 years old (330 settled Bantu and 170 semi-nomadic BaAka Pygmies) inhabiting the villages in the Dzanga Sangha region (south-west CAR) in March 2020. 32.4% of asymptomatic Bantu and 40.6% of asymptomatic Pygmy children had a positive result of malaria RDT. The mean age of the study participants with RDT (+) was 8.7 in Bantu and 7.0 years in Pygmies; the mean body temperature was 36.8oC in both groups; the mean haemoglobin level was 10.6 g/dL and 10.1 g/dL, respectively. Our findings allowed us to demonstrate the high prevalence of asymptomatic malaria infections in south-west CAR. RDTs seem to be a useful tool for the detection of Plasmodium falciparum in areas with limited possibilities of using other diagnostic methods, such as light microscopy and molecular biology.

Fibroblasts, the most abundant cells in the connective tissue, are key modulators of the extracellular matrix (ECM) composition. These spindle-shaped cells are capable of synthesizing various extracellular matrix proteins and collagen. They also provide the structural framework (stroma) for tissues and play a pivotal role in the wound healing process. While they are maintainers of the ECM turnover and regulate several physiological processes, they can also undergo transformations responding to certain stimuli and display aggressive phenotypes that contribute to disease pathophysiology. In this review, we focus on the metabolic pathways of glucose and highlight metabolic reprogramming as a critical event that contributes to the transition of fibroblasts from quiescent to activated and aggressive cells. We also cover the emerging evidence that allows us to draw parallels between fibroblasts in autoimmune disorders and more specifically in rheumatoid arthritis and cancer. We link the metabolic changes of fibroblasts to the toxic environment created by the disease condition and discuss how targeting of metabolic reprogramming could be employed in the treatment of such diseases. Lastly, we discuss Systems Biology approaches, and more specifically, computational modelling, as a means to elucidate pathogenetic mechanisms and accelerate the identification of novel therapeutic targets.

In this study a method for predicting the preferred pleasantness induced by different forest environments, represented by virtual photographs, was proposed and evaluated using a novel Anti-Environmental Forest Experience Scale psychometric test. The evaluation questionnaire contained twenty-one items divided into four different subscales. The factor structure was assessed in two separate samples collected online (sample 1: N = 254, sample 2: N = 280). The internal validity of the four subscales was confirmed using an exploratory factor analysis. Discriminant validity was tested and confirmed using the Amoebic Self Scale (Spatial-Symbolic domain). Concurrent validity was confirmed using the Connectedness to Nature Scale. Predictive validity was based on assessment of pleasantness induced by nine different photographs (control – urban landscapes, forest landscapes, dense forest landscapes), with subscales differently correlated with the level of pleasantness assessed for each photograph. This evaluation instrument is appropriate for predicting preferred pleasantness induced by different forest environments.

Only now, four decades after passive houses were designed in the US and built in Canada, the authors are able to discuss the next generation of new and retrofitted buildings in the same way. This paper presents a universal system for different types of buildings and climates that includes construction experience and is reinforcing multi-disciplinary synergies. A next generation of technology uses adaptable indoor climate, integrates HVAC and building structure; applies field monitoring of energy and indoor environment and develops a performance model that is based on artificial neutral network. This approach, includes: 1. The self-learning ANN as a part of the building management system, 2. This management system guides energy optimization in a post-construction stage 3. A two-stage construction process for new and retrofits. The first stage prescribes investment level and optimizes performance; opposite in the second stage. This paper highlights that some elements of the proposed methodology have already been applied in the practice. It also underlines that this integrated multi-disciplinary system can be applied with a different combination of technological elements.

The current COVID-19 pandemic is one of the most devastating events in recent history. The virus causes relatively minor damage to young, healthy populations, imposing life-threatening danger to the elderly and people with diseases of chronic inflammation. Therefore, if we could reduce the risk for vulnerable populations, it would make the COVID-19 pandemic more similar to other typical outbreaks. Children don’t suffer from COVID-19 as much as their grandparents and have a much higher melatonin level. Bats are nocturnal animals possessing high levels of melatonin, which may contribute to their high anti-viral resistance. Viruses induce an explosion of inflammatory cytokines and reactive oxygen species, and melatonin is the best natural antioxidant that is lost with age. The programmed cell death coronaviruses cause, which can result in significant lung damage, is also inhibited by melatonin. Coronavirus causes inflammation in the lungs which requires inflammasome activity. Melatonin blocks these inflammasomes. General immunity is impaired by anxiety and sleep deprivation. Melatonin improves sleep habits, reduces anxiety and stimulates immunity. Fibrosis may be the most dangerous complication after COVID-19. Melatonin is known to prevent fibrosis. Mechanical ventilation may be necessary but yet imposes risks due to oxidative stress, which can be reduced by melatonin. Thus, by using the safe over-the-counter drug melatonin, we may be immediately able to prevent the development of severe disease symptoms in coronavirus patients, reduce the severity of their symptoms, and/or reduce the immuno-pathology of coronavirus infection on patients’ health after the active phase of the infection is over.

As the global demand for water increases, so does the quantity of wastewater requiring treatment. Due to a relatively low carbon footprint, compared with conventional wastewater treatment technologies, anaerobic digestion (AD) was identified in the 1970s as a forerunner in the push for sustainability, when interest in sustainable technologies and renewable energy sources was first sparked. AD technology development ultimately resulted in the discovery of the ‘anaerobic granule’. It is a spontaneously-forming bio-aggregate of microbial cells capable of digesting pollutants and producing methane-rich biogas as a renewable source of bioenergy. The high settling velocity of such granules meant that AD systems could be operated as high-rate treatment processes, because the active, relatively-slow-growing, pollutant-removing biomass would be retained inside, and not washed out of, even bioreactors operated at extremely high volumetric loading rates. In the intervening years the emergence of the anaerobic ammonium oxidising (anammox) granule, aerobic granule, hydrogenic granule, oxygenic photogranule, and many other functionally-specialised granules, has opened new opportunities in wastewater treatment biotechnology. Whilst environmental engineering based around wastewater treatment is still a growing field of research and implementation, the granule (in all forms) is starting to catch the attention of microbial ecologists. It is a self-immobilised biofilm, with many of the properties of ‘conventional’ biofilms formed in Nature. However, as a single entity, a granule represents an entire community of microorganisms, competing or functioning cooperatively or in syntrophy. Together, inside a bioreactor, granules perform side-by-side arguably representing a meta-organism. Granules are gaining traction as the perfect samples for high-throughput studies on fundamental ecological concepts. Granular biofilms can be used to test hypotheses around drivers of diversity, community assembly, biofilm formation and maturation, community expansion and succession, community stress response, among others. This review outlines the history of three of the most influential types of granules: the anaerobic (methanogenic), aerobic and anammox granule. The main biochemical processes found in each type; their primary characteristics; and the typical makeup of the microbial community underpinning the processes are compared. Finally, the adoption of granules as the perfect ‘playground’ for experiments in microbial ecology is reviewed.

The increase in arrivals of new forest pests highlights the need for effective phytosanitary legislation and measures. This paper introduces legislation targeted at prevention and management of potential introductions of forest pests and pathogens. A summary is given on plant health regulations on a global and regional level with detailed information on the situation in the European Union (EU). The current and new European legislation is discussed and a particular focus is given on eradication and contingency plans for Fusarium circinatum.

Traditionally, palliative care (PC) systems focused on the needs of advanced cancer patients. But, most patients needing PC have end-stage organ diseases. Similarly, PC models focused on the needs of patients in hospice or at home; however, in most cases PC is provided in acute hospitals. Indeed, the symptom burden that these patients experience in the last year of life frequently forces them to seek care in Emergency Department. The majority of them are admitted to the hospital and many die. This issue poses important concerns. Despite the efforts of attending healthcare professionals, inhospital patients do not receive optimal care near the end-of-life. Also, evidence is emerging that delay in identifying patients needing PC have a detrimental impact on their quality of life (QoL). Therefore, there is an urgent need to early and properly identify these patients among those hospitalized. Several trials reported the efficacy of PC in improving the QoL in these patients. Each hospital should ensure that a multidisciplinary PC team is available to support attending physicians to achieve the best QoL for both PC patients and their families. This review discusses the role and the impact of inhospital PC in patients with end-stage disease or advanced cancer.

The topic of modification of magnesium oxychloride cement (MOC) via specific functional additives is very much pronounced in the research of alternative building materials. This study deals with the co-doping of MOC by 1D and 2D carbon nanomaterials in order to improve its mechanical properties while using tannic acid (TA) as a surfactant. Furthermore, the effect of TA on MOC also covers the improvement of water resistance. As a filler, three size fractions of standard quartz sand are used. The proposed types of MOC-based composites show promising results considering their mechanical, macro- and microstructural, chemical, and hygric properties. The use of 1D and 2D nanoadditives and their mixture enables the improvement of the flexural strength and most of all softening coefficient, the durability parameter characterizing the resistance of the prepared materials to water. For samples immersed in water for 24 h, the compressive strength of all modified composites tested was higher than that of the reference composite. Quantitatively, the developed co-doped composite shows mechanical parameters comparable to or even better than those of commonly used Portland cement-based materials, while maintaining high environmental efficiency. This indicates its potential use as an environmentally friendly alternative to Portland cement-based products.

Background: Human epidermal growth factor receptor 2 (HER-2) overexpression can be found in 15-20% of breast cancers, and it strongly correlates with aggressive clinical behavior and adverse prognosis. The first-line treatment for HER-2 positive metastatic breast cancers is the combination of trastuzumab, pertuzumab, and taxane (PTH). ABP 980 is a biosimilar of the innovator trastuzumab and is characterized by highly comparable effectiveness. Methods: The group of 61 patients with HER-2 positive MBC received biosimilar ABP 980 plus pertuzumab and docetaxel from November, 18, 2018 to December, 24, 2019. The response to therapy, overall survival (OS), progression-free survival (PFS), metastases, and adverse effects among patients were determined and analyzed. Results: Initially, 42 women responded partially to the treatment and their median PFS was 27 months. Median PFS for the whole group was 18 months. Cardiotoxicity of treatment was noticed in all patients in the form of the reduction in left ventricular ejection fraction but only in 2 cases, it was the reason for withdrawing from therapy. Conclusion: Biosimilar ABP 980 is registered in the same indications as the innovator trastuzumab and their effectiveness, as well as side effects, are comparable. The costs of biosimilar make the therapy more accessible and thus more patients with MBC around the world can receive relevant treatment.

There is a need to further characterize the antibody response to vimentin in relation to it possible involvement in pathogenicity of sarcoidosis, and other lung disorders. Objectives: We investigated serum samples from patients with sarcoidosis, healthy and diseased control subjects to evaluate levels and frequency of these antibodies. Materials and methods. A retrospective-prospective comparative study was performed in the years 2014-2017. 188 serum samples were examined for presence of autoantibodies to mutated citrullinated vimentin (anti-MCV). Patients with elevated anti-MCV levels were tested for antibodies to a cyclic citrullinated peptide (anti-CCP) and citrullinated vimentin (anti-Sa). Additionally, sera from 93 patients with sarcoidosis and 40 healthy subjects were evaluated for the presence of autoantibodies to non-modified vimentin. In all cases ELISA assays was used. The results were considered statistically significant at p-value less than 0.05. Results of the study. The high concentrations of anti-MCV. antibodies were more frequent in patients with sarcoidosis (40.9% of the cases, 38/93), compared to the control groups (23.6% and 25.0% of cases, respectively). In sarcoidosis, clinical symptoms similar to the autoimmune pathology were described. A moderate positive correlation between the anti-MCV and anti-Sa antibodies (r = 0.66) was found in 13 patients with sarcoidosis. There was no significant difference between the levels of the anti-MCV and the anti-CCP in patients with non-infectious lung diseases and the healthy control group. Conclusion. A high level of anti-MCV antibodies in patients with sarcoidosis characterizes the presence of an autoimmune process that is not related to citrullination. The absence of a significant difference in the level of antibodies to modifications of vimentin in patients with sarcoidosis may indicate the autoimmune process.

Social interaction is essential for life and is impaired in many psychiatric disorders like schizophrenia, au-tism, depression and major anxiety disorder. Monoamine transmission plays a key role in social behavior and both genetic and epigenetic modifications of dopamine and noradrenaline neurotransmission-related genes can affect the levels of social interaction. Since heterozygous individuals for a specific genetic trait possess only one mutant allele of that trait, in order to better evaluate the role of the interaction between genetics and epigenetics in unmasking latent genetically-determined predispositions, our interest has focused on studying the interplay between genetics and epigenetics influences on social behavior in male rats obtained by two different breeding schemes: a first group by breeding of knock-out (KO) male rats with wild-type (WT) female dams (homogeneous heterozygous offspring, termed MAT-HET), and a second group of heterozygous DAT male offspring by breeding of KO male and DAT-heterozygous female subjects (to obtain comparable control pups, termed MIX-HET). Their social behavior was then assessed by partner preference, social preference and elicited preference tests. In the first test MIX-HET and MAT-HET male mice had choice between two WT females one in estrous and the other not in estrous. In the second test they met either a MIX-HET or a WT male rodent. Also, the expression of the noradrenaline transporter (NET) was assessed in the prefrontal cortex, hippocampus and hypothalamus of MAT, MIX and WTs by immunofluorescence in order to estimate its involvement in the expression of social behavior. Our results show that MIX-HET focal rodents tend to have an asocial behavior when in contact with a female in estrous, and their behavior is similar to when the stimulus is a MIX-HET male. MAT-HET male rodents, instead, tend to be very attracted by the female in estrous, but they ignore the MIX-HET stimulus. MIX-HET progeny showed a lower expression of noradrenaline transporter in both hypothalamus and hippocampus with respect to MAT-HET rats, whereas MAT-HET rats displayed increased noradrenaline transporter immunofluorescence in the hypothalamus and in the hippocampus with respect to WT rats, while no difference was observed in the prefrontal cortex. Therefore we can hypothesize that the differences observed between the two heterozygous groups may be attributable to an epigenetic factor: the different maternal care received. These data can open new perspectives towards increased the preclinical knowledge about autism and bipolar disorder.

The study is dedicated to analyzing the complex interaction between Artificial Intelligence (AI) and the Internet of Things (IoT) with institutional structures in the context of sustainable development. This work examines the impact of these technologies on economic and social development, identifying their potential advantages and risks. Methodologically, the research relies on information gathering and case study analysis, assessing the impact of technology in various domains, from smart cities to the manufacturing sector. The findings emphasize the necessity of integrating technological innovations into broader social and environmental initiatives, highlighting the importance of institutional transformations for achieving environmental sustainability.

We investigate the shear quasinormal modes (QNMs) in the context of electron stars, focusing on the Lifshitz geometry and exploring the behavior in the small star limit. The study begins by analyzing the shear sector modes in the infrared (IR) Lifshitz limit, where the geometry approaches that of a pure Lifshitz space. We derive and solve the differential equations governing the shear modes, revealing the asymptotic behavior in terms of the Lifshitz exponent. In the special case of integer values of the exponent, we obtain analytic corrections to the solutions to extract hydrodynamical QNMs. We then explore the cases with a Lifshitz exponent equal to or greater than three, providing analytical corrections to the Lifshitz IR solutions. A special case with an exponent of two is also considered, leading to modified solutions. The study extends to finding hydrodynamical QNMs, emphasizing the role of the Lifshitz geometry. Further investigations involve the flux with real and complex frequencies, considering the off-shell Lagrangian and deriving conserved flux expressions. Our analysis focuses on the exterior of the star, modeled by the Reissner-Nordström-AdS geometry. The small star limit is examined, revealing interesting features in the behavior of fermionic excitations. In summary, we provide a detailed exploration of shear quasinormal modes in electron stars, shedding light on their behavior in Lifshitz geometry, small star limits, and the interplay between real and complex frequencies. Our findings contribute to our understanding of the dynamics of electron stars and their gravitational properties.

We explore the relationship between the exact renormalization group (RG) equation and quantum mechanics. The central idea is to rewrite the exact Wilsonian RG equation as a Schrödinger-type equation, opening avenues for analysis. By introducing a functional transformation, the RG equation takes on a form analogous to a quantum mechanical Schrödinger equation. The study investigates solutions to this equation and draws parallels with quantum mechanics, paving the way for understanding the infrared behavior of quantum field theories. Furthermore, we extends its insights to a one-dimensional toy model, providing illustrative examples. The discussion encompasses the implications of these findings in multiple dimensions and quantum field theory, with a brief exploration of their relevance in the context of inflationary cosmology.

The limits for recording weak signals for electrochemical seismic sensors are determined by their self-noise. At the same time, the noise nature is complex and not fully understood. In this work, for the first time, in addition to the frequency dependences of the noise amplitude, the difference in the phases of the noise currents of the cathodes of an electrochemical sensor has been studied. It has been established that at low frequencies the cathode currents change in an-tiphase. As the frequency increases, the phase difference becomes random. At the same time, the frequency at which the phase difference changes depend on the interelectrode distance; the clos-er the electrodes are located to each other, the greater it is. Physically, this effect is explained by the transition from noise caused by the integral flow of the working fluid to noise associated with vortex flows, the size of which depends on frequency. Thus, a new way of studying noise makes it possible to identify its physical nature and develop new methods of reduction.

Marine Protected Areas (MPAs) are vital for biodiversity conservation, yet their effectiveness in preserving foundation seaweeds remains understudied. This study investigates the diversity and distribution of Cystoseira sensu lato (including Cystoseira, Ericaria and Gongolaria) populations in the an MPA located in the central Mediterranean Sea, comparing them with those in two unprotected control sites. We hypothesize that MPA Cystoseira s.l. populations will display higher diversity and structure compared to outside control sites. Results reveal a total of 19 Cystoseira s.l. species at depths of 0-20 m, with the MPA exhibiting a higher diversity than control sites. Thus, MPAs can play a crucial role in fostering the diversity of Cystoseira s.l. populations. However, no significant differences were observed among the MPA's protection zones, raising questions about the zoning effectiveness. Additionally, our survey uncovered a substantial presence of non-indigenous seaweeds within the MPA. In conclusion, while MPAs improve Cystoseira s.l. diversity compared to unprotected sites, the varying efficacy of protection within MPA zones suggests a necessity for site-specific conservation strategies. The presence of non-indigenous seaweeds emphasizes ongoing challenges. This study provides a baseline for understanding Cystoseira s.l. population dynamics, crucial for future monitoring and conservation efforts in the face of climate change.

Currently, the district heating (DH) in Poland is facing many challenges. The business model used hitherto was very simple—in most cities, heating plants produced heat, and centralized heating systems supplied the "product" at a price approved annually by the president of the Energy Regulatory Office (URE). However, recent years have brought significant changes on the market. EU regulations force the elimination of old coal-fired plants that were still built in the Polish People's Republic (PRL), moreover, high prices of CO2 emission allowances aggravate the financial situation of companies. In addition, in the heating sector, the trend observed in the power sector is becoming increasingly visible—limiting the role of large sources in favor of energy generated locally, closer to the customer. One of the biggest challenges is achieving the targets set by the EU for the share of renewable energy sources (RES) in the heating sector. The war in Ukraine and problems with the supply of coal and gas are an additional impulse to turn to RES. Thus, the development of sustainable and innovative solutions for energy production and supply at the level of urban networks is currently one of the main technical challenges. The purpose of the paper is to present the current situation and perspectives of development of district heating systems in Poland with a view to the status of district heating in the world, and some deeper insight into European conditions. The review presents energy sources with particular emphasis on renewable energy sources (RES) and their cogeneration for heat production. The examples of existing heating network solutions using renewable energy sources, based on the selected published case studies, are also discussed.

Due to the rapid emergence of multi drug resistant (MDR) pathogens against which current an-tibiotics are no longer functioning, severe infections are becoming practically untreatable. Con-sequently, the discovering of new classes of effective antimicrobial agents with novel modes of action is becoming increasingly urgent. The bioactivity of Cannabis sativa, an herbaceous plant used for millennia for medicinal and recreational purposes, is mainly due to its content in phytocan-nabinoids (PCs). Among the 180 PCs detected, cannabidiol (CBD), Δ8 and Δ9-tetrahydrocannabinols (Δ8-THC and Δ9-THC), cannabichromene (CBC), cannabigerol (CBG), cannabinol (CBN) and some their acidic precursors have demonstrated from moderate to potent antibacterial effects against Gram-positive bacteria (MICs 0.5–8 µg/mL), including methicillin-resistant Staphylococcus aureus (MRSA), epidemic MRSA (EMRSA), as well as fluoroquinolones and tetracycline-resistant strains. Particularly, the not psychotropic CBG was also capable to inhibit MRSA biofilm formation, to eradicate that mature, and to rapidly exterminate MRSA persiters cells. On this scenario, CBG, as well as other minor not psychotropic PCs, such as CBD, and CBC could represent promising compounds for developing novel antibiotics with high therapeutic potential. Anyway, further studies are necessary, needing abundant quantities of such PCs, scarcely provided naturally by Cannabis plants. Here, after an extensive overture on cannabinoids including their reported an-timicrobial effects, aiming at easing the synthetic production of the necessary amounts of CBG, CBC and CBD for further studies, we have, for the first time, systematically reviewed the synthetic pathways utilized for their synthesis, reporting both reaction schemes and experimental details.

Melting phase relations of the diamond-forming system olivine (Ol) – jadeite (Jd) – diopside (Di) – (Mg, Fe, Ca, Na)-carbonates (Carb*) – (C-O-H-fluid) are studied in experiments at 6.0 GPa in the polythermal section Ol74Carb*18.5(C-O-H)7.5 - Omp74Carb*18.5(C-O-H)7.5, where Ol = Fo80Fa20, Omp (omphacite) = Jd62Di38 and Carb* = (MgCO3)25(FeCO3)25(CaCO3)25(Na2CO3)25. Peritectic reaction of olivine and jadeite-bearing melt with formation of garnet has been determined as a physico-chemical mechanism of ultrabasiс-basiс evolution of the diamond-forming system. In the process CO2-component of the supercritical C-O-H-fluid can react with silicate components to form additional carbonates of Mg, Fe, Ca and Na. Solidus temperature of the diamond-forming system is depressed to 1000-1020°C by joint effect of the H2O-fluid and carbonate constituents thereof. The experimentally recognized peritectic mechanism of ultrabasiс-basiс evolution of the diamond-forming system explains the origin of the associated paragenetic inclusions of peridotite and eclogite minerals in diamonds as well as the xenoliths of diamond-bearing peridotites and eclogites of the kimberlitic deposits of diamond. The diamond-forming systems have formed with the use of material of the upper mantle native peridotite rocks. In this case the capacity of the rocks to run the peritectic reaction of olivine was transmitted with silicate components to the diamond-forming systems.

The standard edge crush test (ECT) allows to determine the crushing strength of the corrugated cardboard. Unfortunately, this test cannot be used to estimate the compressive stiffness, which is an equally important parameter. It is because, any attempt to determine this parameter using current lab equipment quickly ends in a fiasco. The biggest obstacle is obtaining a reliable measurement of displacements and strains in the corrugated cardboard sample. In this paper, we present a method that not only allows to reliably identify the stiffness in the loaded direction of orthotropy in the corrugated board sample, but also the full orthotropic material stiffness matrix. The proposed method uses two samples: (a) traditional, cut crosswise to the wave direction of the corrugated core, and (b) cut at an angle of 45 degrees. Additionally, in both cases, an optical system with digital image correlation (DIC) is used to measure the displacements and strains on the outer surface of samples. The use of a non-contact measuring system allows to avoid using the measurement of displacements from the crosshead, which is burdened with a large error. Apart from the new experimental configuration, the article also proposes a simple algorithm to quickly characterize all sought stiffness parameters. The obtained results are finally compared with the results obtained in the homogenization procedure of the cross-section of the corrugated board. The results were consistent in both cases.

In the modern world, all manufacturers strive for the optimal design of their products. This general trend is recently also observed in the corrugated board packaging industry. Colorful prints on displays, perforations in shelf-ready-packaging and various types of ventilation holes in trays, although extremely important for ergonomic or functional reasons, weaken the strength of the box. To meet the requirements of customers and recipients, packaging manufacturers outdo each other in new ideas for the construction of their products. Often the aesthetic qualities of the product become more important than the attention to maintaining the standards of the load capacity of the packaging (which, apart from their attention-grabbing functions, are also intended to protect transported products). The particular flaps design (both top and bottom) and their influence on the strength of the box is investigated in this study. The updated analytical-numerical approach is used here to predict the strength of the packaging with various flap’s offsets. Experimental results indicated a significant decrease in the static load-bearing capacity of packaging in the case of shifted flap creases. The simulation model proposed in our previous work has been modified and updated to take into account also this effect. The results obtained by the model presented in the paper are in satisfactory agreement with the experimental data.

The corrugated board packaging industry is increasingly using advanced numerical tools to design and estimate the load capacity of its products. That is why numerical analyzes are becoming a common standard in this branch of manufacturing. Such trend causes either the use of advanced computational models that take into account the full 3D geometry of the flat and wavy layers of corrugated board, or the use of homogenization techniques to simplify the numerical model. The article presents theoretical considerations that extend the numerical homogenization technique already presented in our previous work. The proposed here homogenization procedure also takes into account the creasing and / or perforation of corrugated board, i.e. processes that undoubtedly weaken the stiffness and strength of the corrugated board locally. However, it is not always easy to estimate how exactly these processes affect the bending or torsional stiffness. What is known for sure is that the degradation of stiffness depends, among other things, on the type of cut, its shape, the depth of creasing, as well as their position or direction in relation to the corrugation direction. The method proposed here can be successfully applied to model smeared degradation in a finite element or to define degraded interface stiffnesses on a crease line or a perforation line.

The need for quick and easy deflection calculations of various prefabricated slabs causes that simplified procedures and numerical tools are used more and more often. Modelling of full 3D finite element (FE) geometry of such plates is not only uneconomical but often requires the use of complex software and advanced numerical knowledge. Therefore, numerical homogenization is an excellent tool, which can be easily employed to simplify a model, especially when accurate modelling is not necessary. Homogenization allows for simplifying a computational model and replacing a complicated composite structure with a homogeneous plate. Here, a numerical homogenization method based on strain energy equivalence is derived. Using the method proposed, the structure of the prefabricated concrete slabs reinforced with steel spatial trusses is homogenized to a single plate element with an effective stiffness. There is a complete equivalence between the full 3D FE model built with solid elements combined with truss structural elements and the simplified homogenized plate FE model. The method allows for the correct homogenization of any complex composite structures made of both solid and structural elements, without the need to perform advanced numerical analyses. The only requirement is a correctly formulated stiffness matrix of a representative volume element (RVE) and appropriate formulation of the transformation between kinematic constrains on RVE boundary and generalized strains.

This review will explore the four major pillars required for design and development of an saRNA vaccine: antigen design, vector design, non-viral delivery systems, and manufacturing (both saRNA and lipid nanoparticles (LNP)). In will report on the major innovations, preclinical and clinical data reported in the last five years and will discuss future prospects.

The paper presents the influence of different curing conditions – wet, dry and protection against water evaporation on selected properties of concretes with different amount of recycled concrete aggregate previously subjected to atmospheric CO2 sequestration. Additionally, the eco-efficiency bi and ci indexes as well as eco-durability S-CO2 index were calculated. It was found that dry conditions deteriorate the properties of concrete, especially made of blast furnace slag cement, while protection against evaporation allows to achieve results comparable to wet conditions. Moreover, for series with the highest amount of coarse recycled aggregate and after longer period of curing, the difference between the effects of wet curing and protection against water evaporation disappears. The eco-efficiency and eco-durability indexes approach confirms the beneficial effect of blast-furnace slag cement used as a binder but on condition of proper way of curing.

The aim of this study was to investigate the size of the change in fatigability of gluteus maximus muscles during difficult endurance training in dynamic conditions. The research covered involved eight female athletes of the Polish National Team in short track, which had been prepared to the Olympic Games in PyeongChang. The sEMG system was used to measure fatigue of right and left gluteus maximus muscles, in the modified Biering-Sorensen test. The test was conducted five times during the training: before training, after warmup, and after each of 3 series of the endurance training. Comparing the mean frequency of the surface electromyography power spectrum of the first and the last seconds of the test, statistically significant reduction (p<0.05) of the average frequency value of the right muscle from 55.61±7.08 Hz to 48.64±4.48 Hz and left muscle from 58.78±4.98 Hz to 53.18±4.62 Hz was reported. In the conducted Biering-Sorensen test, the value of the muscle signal frequency measured by surface electromyography decreases, which may prove the muscle fatigue. Reductions in the frequency measured in the first and the last second of the test, was higher of the right lower limb. The size of the d Cohen effect in fatigue drops along with subsequent five tests during the training.

Mediterranean climate is marked by arid climate conditions in summer, therefore, crop irrigation is crucial to sustain plant growth and productivity in this season. If groundwater is utilized for irrigation, an impressive water pumping is needed to satisfy crop water requirements at catchment scale. Consequently, irrigation water quality gets worse, specifically considering groundwater salinization near the coastal areas due to seawater intrusion, also triggering soil salinization. With reference to an agricultural coastal area in the Mediterranean basin (Southern Italy), close to the Adriatic sea, an assessment of soil salinization risk due to processing tomato cultivation was carried out. A simulation model was arranged to perform, on daily basis, a water and salt balance along the soil profile. Long-term weather data and soil physical parameters representative of the considered area were utilized in applying the model, also considering three salinity levels of irrigation water. Based on the climatic analysis performed and the model outputs, the probability of soil salinity came out very high, such as to seriously threaten tomato yield. Autumn-winter rainfall resulted frequently insufficient to leach excess salts away from the soil profile and reach sustainable conditions of tomato cultivation. Therefore, alternative cropping strategies were prospected.

Here, we report a simple and effective method for separation of gram-negative bacteria using aptamer-modified microbeads and acoustophoresis. As acoustophoresis allows for simultaneous washing and size-dependent separation in continuous flow mode, we efficiently obtained gram-negative bacteria that showed high affinity without any additional washing steps. The proposed device has a simple and efficient channel design, utilizing a long, square-shaped microchannel that shows excellent separation performance in terms of the purity, recovery, and concentration factor. Microbeads (10 µm) coated with the GN6 aptamer can specifically bind gram-negative bacteria. Using acoustophoresis, gram-negative bacteria-bound microbeads and other unbound/contaminants can be separated by size with high purity and recovery. The device demonstrated excellent separation performance, with high recovery (up to 98%), high purity (up to 99%), and a high volume rate (500 µL/min), and a concentration factor of up to 20×. The acoustophoresis microfluidic device also showed binding affinity to multiple strains of gram-negative bacteria, but not to gram-positive bacteria. This study presents a new paradigm for early diagnosis of bacterial infectious diseases. In addition to detecting living bacteria or bacteria-derived biomarkers, this protocol can be extended to monitoring the contamination of water resources, and may aid quick responses to bioterrorism and pathogenic bacterial infections.

The aim of the study was to examine the long-term prognostic value of changes in cardio-ankle vascular index (CAVI) within a year after coronary artery bypass grafting (CABG). Methods. Patients with coronary artery disease (n = 251) in whom cardio-ankle vascular index (CAVI) was assessed using the VaSera VS-1000 device before and in a year after CABG. Groups with improved CAVI (CAVI decreased from pathological value to normal or the index remained within normal values) or worsened CAVI (persistent pathological index value or an increase in CAVI to pathological values or an increase of 1 unit or more) were identified. The groups were compared for the following events at 10-year follow-up: death from all causes, myocardial infarction, stroke/transient ischemic attack, and the combined endpoint. Results. A year after CABG surgery, 45% of patients showed positive dynamics or stable normal CAVI values. Death from all causes was significantly more common in the group with CAVI progression - 32 (27.6%) than in the group with CAVI improvement - 14 (14.8%; p = 0.029). Patients with CAVI progression were more likely to have MACE (death, MI, stroke/TIA) in 49 (42.2%) cases, compared with patients with CAVI improvement - in 23 (24.5%; p=0.008). Negative dynamics of CAVI (p = 0.024), number of shunts (p = 0.006), and the presence of carotid stenosis more than 30% (p = 0.051) were independent predictors of death from all causes at 10-year follow-up after CABG. The presence of carotid stenosis more than 30% (p = 0.002) and the group with negative dynamics of CAVI after a year (p = 0.008) were independent predictors of the development of the combined endpoint during long-term follow-up. Conclusions. Patients with negative dynamics of CAVI one year after CABG were more likely to experience death from all causes and combined endpoint in the long-term follow-up than patients with normal CAVI. In further studies, it is advisable to evaluate the dynamics of CAVI and, based on this, identify the most effective interventions to improve the prognosis of patients after CABG.

In the empirical dimension, the authors' intention was to define the criteria that should be taken into account by universities to meet the requirements of sustainable university development - in terms of activities and reporting through the analysis of good practices developed by top Polish universities. The aim was also to select activities that, based on benchmarks, could be used by universities at every stage of implementing SDG goals. This is not an easy task, considering that for educational institutions the priority is the educational process, and the implementation of the principles of sustainable development is only possible through an intensive educational process combined with the application of knowledge in practice. This is a challenge for the university because it requires remodeling the current culture, changes in processes and the perception of certain cultural and social phenomena, and will involve a change in the teacher-student relationship, a change in the traditional education process to practice-based education, and will also require an interdisciplinary context. in the content provided. But this is precisely the challenge for outstanding educators. The authors compared three significant world rankings to which Polish universities also report. They collected information and data mainly regarding the activities undertaken by the reporting Polish universities in the area of ​​developing good practices. Many ideas and tips will enable the preparation of further material, which the authors plan to deepen with a detailed analysis of SD indicators.

Sumatriptan is a serotonin receptor agonist approved by FDA in 1992 to treat migraines. Its’ solid form used in commercially available drugs Imitrex, Treximet, Onzetra Xsail and Zembrace symtouch is sumatriptan succinate. Herein we report on crystal structure of sumatriptane succinate hemi(ethanol solvate), HSum+·HSucc–·0.5EtOH, its comparison with previously reported solids containing derivatives of sumatriptan.

In order to ensure sustainable tourism development, it is currently becoming crucial to look into promotion of popular tourist destinations in Russia. This is due to a number of factors, including the acceptance of the credibility and importance of the concept of sustainable tourism, as well as the awareness of the necessity of taking into account a wide range of short- and long-term effects, external factors, and interdisciplinary aspects of the development of the Russian tourism industry. In the context of a local tourist destination during the economic crisis, the study aims to highlight some of the key economic elements of sustainable tourism. The decision to adopt a regional perspective is necessitated by the fact that many of the most crucial elements for the long-term sustainable development of the tourist complex are established at this level, including decision-making by businesses and authorities that are economically viable. The research focuses on the economic aspects to be considered while designing an overall strategy for the long-term development of regional tourist destinations in Russia. The authors define sustainable tourism from the viewpoint of economics. This understanding extends beyond the relatively limited framework of environmental and socioeconomic consequences of tourism development, affecting some less obvious economic aspects of a regional destination that directly relate to sustainable development.

Radiological imaging is an essential component of a swallowing assessment. Artificial intelligence (AI), and especially deep learning (DL) models, have enhanced the efficiency and efficacy through which imaging is interpreted, and subsequently have important implications for swallow diagnostics and intervention planning. However, the application of AI for the interpretation of videofluoroscopic swallowing studies (VFSS) is still emerging. This review showcases recent literature in the use of AI to interpret VFSS and highlights clinical implications for speech pathologists (SPs). With a surge in AI research, there have been advances made in dysphagia assessment. Several studies have demonstrated successful implementation of DL algorithms to analyze VFSS. Notably, convolutional neural networks (CNNs) have been used to detect pertinent aspects of the swallowing process with high levels of precision. DL algorithms have the potential to streamline VFSS interpretation, improve efficiency and accuracy, and enable precise interpretation of instrumental dysphagia evaluation, which is especially advantageous when access to skilled clinicians is not ubiquitous. By enhancing precision, speed, and depth of VFSS interpretation, SPs can obtain a more comprehensive understanding of swallow physiology and deliver targeted and timely intervention that is tailored towards the individual. This has practical applications for both clinical practice and dysphagia research. As this research area grows and AI technologies progress, the application of DL in the field of VFSS interpretation is clinically beneficial and has the potential to transform dysphagia assessment and management. With broader validation and inter-disciplinary collaborations, AI-augmented VFSS interpretation is likely to transform swallow evaluation and ultimately improve outcomes for individuals with dysphagia.

Background: This study investigated the incidence, characteristics and functional outcomes associated with unplanned Acute Care Unit Readmissions (ACUR) during inpatient traumatic brain injury (TBI) rehabilitation in an Asian cohort. Methods: Retrospective review of electronic medical records from single rehabilitation unit was conducted from 1 Jan 2012 to 31 Dec 2014. Inclusion criteria were first TBI, aged &gt;18 years, admitted &lt; 6 months of TBI. ACUR were characterized into either of neurological, medical or neurosurgical subtypes. The main outcome measure was discharge Functional Independence Measure (FIM). Secondary outcomes included rehabilitation length of stay (RLOS). Results: Of 121 eligible TBI records, the incidence of ACUR was 14% (n=17), comprising neurologic (76.5%) and medical (23.5%) subtypes, occurring at median of 13 days (IQR 6, 28.5) after rehabilitation admission. Patients without ACUR had significantly higher admission mean (SD) FIM score compared to those with ACUR (FIM ACUR-negative 63.4 (21.1) vs FIM ACUR-positive 50.53(25.4), P =0.026). Significantly lower discharge FIM was noted in those with ACUR compared to those without. (FIM ACUR-positive 65.8(31.4) vs FIM ACUR-negative 85.4 (21.1), P = 0.023) Furthermore, a significant near-doubling of RLOS was noted in ACUR patients compared to non-ACUR counterparts [ACUR-positive median 55 days (IQR 34.50, 87.50) vs ACUR-negative median 28 days (IQR 16.25, 40.00), P = 0.002]. Conclusion: This study highlights the significant negative functional impact and lengthening of rehabilitation duration of ACUR on inpatient rehabilitation outcome for TBI.

Silver in different forms is used for medical purposes from ancient times. It is not yet well known, which form of silver is more biocompatible and less toxic. Here we considered silver nanoparticles and silver citrate. Also, the relationships of neurotoxicity of silver compaunds with ageing factor is not yet described. To assess the role of nanoform in neurotoxicity of silver and role of ageing a long-term experiment was conducted. We had four control groups of intact mice and four experimental groups which were exposed to silver nanoparticles and silver citrate for two months. Four groups of mice were introduced into the experiment since the age of five months to assess ageing factors. It was shown that the nanoform does play a certain role in neurotoxicity of silver. Silver citrate seems to be a more preferable silver compound. Ageing can be regarded as a positive factor that neutralizes toxic action of silver compounds. It may be due to the development of physiological/cognitive functions with the age as well as adaptation to unnatural content in the individual cages that is definitely stressful for mice.

Gestational diabetes (GDM) is considered a significant and increasing problem worldwide. The growing body of evidence points out that a hostile intrauterine environment in mothers with GDM via epigenetic mechanisms induces "diabetogenic" and "obesogenic" changes in an offspring's DNA. This sets in motion a vicious intergenerational cycle of metabolic diseases gradually deteriorating the health of the human population. One of the most important players in this process seems to be altered microbiota/microbiome. There is a chance that the identification of specific epigenetic marks may provide a key for future diagnostic, prognostic and therapeutic solutions/measures in the field of person-alized medicine. Given the reversibility of most epigenetic changes, an opportunity arises to improve the long-term health of the human population/race. In this manuscript, we aim to summarize available data on epigenetic changes among women suffering from GDM and their progeny in association with changes in microbiome.

Evidence points to a link between gluten-free diet or celiac disease and eating disorders, but not with orthorexia. This study is the first to examine adults with celiac disease in terms of the prevalence of risk of orthorexia. The study included 130 adults diagnosed with celiac disease. The standardized ORTHO-15 questionnaire was used to assess the risk of orthorexia. Cronbach's alpha test was used to determine the reliability of the ORTHO-15 questionnaire. Eating habits of the subjects were assessed using a questionnaire. The effect of celiac disease on diet was assessed on a 5-point scale. A risk of orthorexia was found in 69% of subjects with celiac disease. A statistically significant (p&lt;0.005) positive correlation was observed between age and ORTHO-15 test scores (rho=0.37). In the group with orthorexia risk, meals were more often self-prepared (93.3%) compared to those without orthorexia (80%) (p=0.023). For 80% of those at risk for orthorexia versus 20% without risk, mood was a factor influencing dietary choices (p=0.001). The study observed a strong association between celiac disease and the presence of orthorexia risk. The numerous risk factors for orthorexia suggest the need for holistic care, including nutritional and psychological support among individuals with celiac disease.

In the modern world, the problem of antibiotic therapy is acute. Despite the diversity of existing antibiotic drugs, their efficacy decreases as new, resistant forms of pathogenic microorganisms emerge. It is extremely difficult to control such processes and even more difficult to treat severe bacterial infections. In such situations, an individual approach to each patient is required and physicians need parameters to estimate the efficacy of antibiotic therapy. This review discusses the significance of monitoring the content of antibiotics in the blood for this purpose, in combination with the content of inflammatory markers, such as C-reactive protein and procalcitonin. The basic principles of antibiotic therapy, and factors in the resistance of microorganisms to antibiotics, are examined. Approaches to assess the efficacy of antibiotic therapy, as well as methods to detect antibiotics and inflammatory markers in the blood of patients, and comparative assessment of their capabilities and limitations, are described.

Reelin depletion and stress seem to affect similar pathways including GABAergic and glutamatergic signaling and both are implicated in psychiatric disorders in late adolescence/early adulthood. The interaction between reelin depletion and stress, however, remains unclear. To investigate this, male and female heterozygous reelin mice (HRM) and wildtype (WT) controls were treated with the stress hormone, corticosterone (CORT), during late adolescence to simulate chronic stress. Glucocorticoid receptors (GR), N-methyl-D-aspartate receptor (NMDAr) subunits, glutamic acid decarboxylase (GAD67) and parvalbumin (PV) were measured in the hippocampus and the prefrontal cortex (PFC) in adulthood. While no changes were seen in male mice, female HRM showed a significant reduction in GR expression in the dorsal hippocampus. In addition, CORT reduced GR levels as well as GluN2B and GluN2C subunits of NMDAr in the dorsal hippocampus in female mice only. CORT furthermore reduced GluN1 levels in the PFC of female mice. The combined effect of HRM and CORT treatment appeared to be additive in terms of GR expression in the dorsal hippocampus. Female-specific CORT-induced changes were associated with overall higher circulating CORT levels in female compared to male mice. This study shows differential effects of reelin depletion and CORT treatment on GR and NMDAr protein expression in male and female mice, suggesting that females are more susceptible to reelin haploinsufficiency as well as late-adolescent stress. These findings shed more light on female-specific vulnerability to stress and have implications for stress-associated mental illnesses with a female bias including anxiety and major depression.

The article discusses the validation process of a certain method of balancing gas contained in the pore space of rocks. The validation was based upon juxtaposition of the examination of rocks' porosity and the effects of comminution in terms of assessing the possibility of opening the pore space. The tests were carried out for six dolomite samples taken from different areas of the 'Polkowice-Sieroszowice’ copper mine in Poland. Before the rock material was ground, it was examined using the porosimetric method and subsequently subjected to the SEM analysis. After grinding, the research material was subjected to the granulometric analysis. Prior to the grinding process, the rocks' porosity fell in the range of 0.3-14.8%, while the volume of the open pores was included in the 0.01-0.06 cm3/g range. The research material was also characterized by inhomogeneous distribution of the pore volume. The grinding process was performed using an original device – the GPR analyzer. The ground samples were characterized by similar particle size distribution and mean diameters D_3.2 of about 4.0-4.5 µm. The SEM analysis revealed pores of various size and shape on the surface of the rock cores, while at the same time demonstrating lack of pores following the grinding process. The grain size distribution curves were compared with the cumulative pore volume curves of the cores before grinding. The resulting intersection points of both curves were seen as testifying to the presence of openings in the rock pores and release of the accumulated gas. The opening percentage of the pore space was associated with the coordinates of the curves' intersections. In order to confirm the argument put forward in this paper – i.e. that comminution of a rock to grains of a size comparable with the size of the rock's pores results in the release of gas contained in the pore space – the amount of gas released as a result of the comminution process was studied. The results of gas balancing demonstrated that the pore space of the investigated dolomites was filled with gas in amounts from 3.19 cm3/kg to 45.86 cm3/kg. The obtained results of the rock material comminution to grains comparable – in terms of size – to the size of the pores of investigated rocks, along with asserting the presence of gas in the pore space of the studied dolomites, were regarded as a proof that the method of balancing gas in rocks via rock comminution is correct.

Understanding the molecular factors involved in the development of uterine myomas may result in the use of pharmacological drugs instead of aggressive surgical treatment. ANGPT1, CASR, and PTK2 were examined in myoma and peripheral tissues samples taken from women after myoma surgery and in normal uterine muscle tissue samples in the control group. Tests were performed using tissue microarray immunohistochemistry. No statistically significant differences in ANGPT1 expression between the tissue of the myoma, the periphery, and the normal uterine muscle tissue of the control group were recorded. The CASR value was reduced in the myoma and peripheral tissue and normal in the group of women without myomas. PTK2 expression was also lower in the myoma and periphery com-pared to healthy uterine myometrium. Calcium supplementation could have an effect on stop-ping the growth of myomas.

The aim of the study was to verify whether a 20 and 40% reduction of nitrogen (N) fertilization rate will be sufficient to maintain soil basic chemical features, fertility, and yielding in monoculture maize cultivation in the no-tillage (NT) system versus the traditional plowing (PL) system. In addition, it was examined which tillage system (PL, NT) allows reduction of fertilization while maintaining good yields of the tested soils. Two fields (10 ha each) were established for PL and NT maize cultivation and soils (0-20 cm) were sampled two times per year - in spring (before maize sowing) and in autumn (after maize harvesting). A broad range of chemical and biological parameters (i.e. pH, forms of nitrogen, phosphorus and carbon, content of selected macronutrients and humic substances, respiration activity) were monitored during the experiment. It was evidenced that the 20% reduction in N fertilization does not have an adverse effect on the chemical and biological characteristics of soils. In turn, the maize yield was mainly the result of both the tillage system and the N rate, i.e. it was significantly higher in the PL system.

The aim of the study was to verify whether a reduced nitrogen (N) fertilization rate according to the EU recommendation (“From field to table” directive) may guarantee good yields and will be sufficient to maintain soil fertility in monoculture maize cultivation in the no-tillage (NT) system versus the traditional plowing (P) system. Two fields (10 ha each) were established for P and NT maize cultivation where the following N doses were applied: 0.0; 69.0, 92.0, and 115.0 kg ha-1. Soils (0-20 cm) were sampled two times per year - in spring (before maize sowing) and in autumn (after maize harvesting). A broad range of chemical (reaction, water holding capacity, total and easily degradable carbon, ammonium, nitrates, magnesium, calcium, potassium, sulfur) and biological (respiration, humic acids) parameters were monitored during the experiment. The vast majority of these factors had higher values in autumn than in spring. Although the role of reduced fertilization was not shown to be significant, the correlation analysis revealed some relationships between yield and nutrient availability. The 20% reduction in N fertilization does not have an adverse effect on the chemistry and biological characteristics of soils and does not worsen their chemical and biological quality.

We identify the source of the Mw = 5.6 earthquake that hit west-central Epirus on March 21, 2020 00:49:52 UTC. We use synthetic aperture radar interferograms tied to one permanent Global Navigation Satellite System (GNSS) station (GARD). We model the source by inverting the INSAR displacement data. The inversion model suggests a shallow source on a low-angle fault (39°) dipping towards east with a centroid depth of 8.5 km. The seismic moment deduced from our model agrees with those of the published seismic moment tensors. This geometry is compatible with the Margariti thrust fault within the collision zone between Apulia and Eurasia. We also processed new GNSS data and estimate a total convergence rate between Apulia and Eurasia of 8.9 mm yr-1 , of which shortening of the crust between the Epirus coastal GNSS stations and station PAXO in the Ionian Sea is equivalent to ~ 50% of it or 4.6 mm yr−1. A 60-km wide deformation zone takes up nearly most of the convergence between Apulia-Eurasia, trending N318°E. Its central axis runs along the southwest coast of Corfu, along the northeast coast of Paxos, heading toward the northern extremity of the Lefkada island.

Background: Influenza vaccine is a tool for preventing infection and reducing exacerbations in patients with asthma and chronic obstructive pulmonary disease (COPD). Purpose of this study is to investigate dynamics of CRP and serum cytokines (IL-2, IL-6, IL-10, IL-17) in patients with asthma and COPD, as well as to perform a correlation analysis with the clinical parameters of the disease manifestation. Methods: The study included 34 patients with asthma, 20 patients with COPD vaccinated against influenza during a period of remission from 2 to 4 weeks, both groups being under a basic maintenance therapy, and 26 healthy individuals vaccinated with the trivalent polymer-subunit vaccine Grippol® Plus, containing 5 µg of influenza virus strains and 500 µg of azoximer bromide. Results: Observing patients with asthma and COPD, during a year after the vaccination, has revealed a significant reduction in exacerbations frequency (p < 0.05) and duration (p < 0.05) of the bronchial obstructive syndrome (BF). Before the vaccination the IL-6 level in patients with asthma had a direct moderate correlation with duration of exacerbations of the underlying disease (p < 0.05), along with the number of systemic corticosteroids courses during exacerbations (p < 0.05). In 12 months after the vaccination, while assessing cytokine profile of all study groups, a significant reduction in the level of IL-6 was observed, compared to the baseline values (p< 0.05). Conclusion: Conducted analysis of immunological, clinical, and functional parameters of patients with asthma and COPD has proven for influenza vaccine to be effective in BF patients.

Background: Over the past two decades, various research teams have designed and applied instruments to measure the quality of life of families with a member who has a disability. A recent systematic review on the state of art of Family Quality of Life in early care identified that many of these studies collected data only from the mothers. The present study aimed to investigate whether there is a bias in participant selection in these types of studies. Method: A systematic review of the scientific literature was conducted in the 3 databases from the year 2000 to 2022. A total of 72 empirical studies have been identified. Results: The findings indicated that most studies examining the family quality of life were based on the information of a single informant per family unit. The profiles of participants according to the research objective is quite similar. In one third of studies the authors report that family members who participate cannot be represented by only mothers or one participant per household. Conclusions: Given the dynamic and collective nature of the construct, the application of a systemic approach is necessary.

Geo-information about the spectral variability of the water-leaving radiance is the key to the validation of satellite and in situ measurements and the development of regional algorithms. In this study, using cluster analysis, five trends were identified that are characteristic of various phenomena in the northwestern part of the Black Sea (summer and winter blooms, turbid waters, river runoff). Typical values of the remote sensing reflectance coefficient are calculated for each case. Additionally, the standard values of the color indices for each cluster are calculated. It is shown that the ratio of the color index CI(412/443 nm) is slightly variable and equals 0.8±0.07.This can be a reference point for recovering incorrect (negative) satellite Rrs(λ) values in the shortwave region. In a similar way, the color index was calculated according to the MODIS and VIRS data, it was shown that on days with a turbid atmosphere (high AOT values), the standard deviation of the color index is 30%. For days with a clear atmosphere, the color index is close to the in situ results.

This paper deals with the structural decomposition input-output for the economic analysis of agriculture, forestry and fishing in six EU-28 countries (Austria, Belgium, France, Germany, Italy and Spain) in the 2010-2015 period. The objective is to determine the causes of changes in production in these sectors with a particular focus on disaggregation technological change by distribution factors associated with a specific normalisation of the Leontief inverse. In calculating the net multipliers, an attempt was made to exclude sectors' own consumption appropriately. However, the treatment of own consumption upon introducing a time factor requires further investigation to avoid questionable quantifications. In general, typical characteristics of primary sectors include the accumulation of a significant amount of their own consumption, facilitated by symmetric accounting. Therefore, attention is drawn to these sectors so as to reveal possible analysis techniques that will provide nuance or validate existing techniques.

Glioblastoma multiforme (GBM) is the most common high-grade intracranial tumor in adults. It is characterized by uncontrolled proliferation, diffuse infiltration due to high invasive and migratory capacities, as well as intense resistance to chemo- and radiotherapy. With a five-year survival of less than 3% and an average survival rate of 12 months after diagnosis, GBM has become a focus of current research to urgently develop new therapeutic approaches in order to prolong survival of GBM patients. The methylation status of the promoter region of the O6-methylguanine–DNA methyltransferase (MGMT) is nowadays routinely analyzed, since a methylated promoter region is beneficial for an effective response to temozolomide-based chemotherapy. Furthermore, several miRNAs were identified regulating MGMT expression, apart from promoter methylation, by degrading MGMT mRNA before protein translation. These miRNAs could be a promising innovative treatment approach to enhance Temozolomide (TMZ) sensitivity in MGMT unmethylated patients and to increase progression-free survival as well as long-term survival. In this review, the relevant miRNAs are systematically reviewed.

In this report, we describe the course and successful treatment of complicated infective endocarditis (IE). A patient presented with a high-grade irregular fever with chills lasting at least 2 months, dyspnoea, chest pain, fatigue, weight loss, and night sweats during the previous 3 months. Above cardiac congenital disorders, he was found to have Granulicatella adiacens infective aortic valve endocarditis, presumably transmitted from the oral cavity niche. Validated metagenomic 16S rDNA next generation sequencing was used to perform taxonomic identification, allowing for specific adequate antibiotic therapy instead of empiric therapy. This paper highlights the critical role of rapid taxonomic identification of nutritionally variant streptococci crucial and the benefit of proper IE treatment to avoid relapses or fatal complications.

The prospective study enrolled 144 patients after surgical treatment of biliary atresia in early infancy. We analyze the immediate effectiveness of the surgery and the age-related structure of complications in the up to 16-year follow-up. The immediate 2-year survival rate after the surgery constituted 49.5%. At the time of this writing, 17 of the patients have celebrated their 10th birthdays with good quality of life and no indications for LT. The obtained results underscore the critical importance of surgical correction of BA by Kasai surgery during the first 60 days of life and subsequent dynamic follow-up of the patients for the purpose of the early detection and timely correction of possible complications.

Ixodes ricinus ticks are widespread in Europe and important vectors for multiple pathogens. The purpose of the study was to collect ticks in a mountainous area in Greece and screen those of I. ricinus species for several pathogens. I. ricinus was the predominant tick species identified (398/440, 90.45%). Following pooling (1-17 ticks per pool), homogenization and extraction of genetic material of the ticks, a real time PCR was applied for the detection of a panel of tick-borne pathogens, while additional conventional PCRs combined with Sanger sequencing were applied for detection of viruses and for typing of Rickettsia and Borrelia species. At least one pathogen was detected in 60 of the 80 (75%) pools. Rickettsia spp. predominated as it was detected in 63.75% of the tick pools (51/80), followed by Borrelia spp. (35 pools, 45%); A. phagocytophilum was detected in 2 (2.5%) pools, phleboviruses in 3 (3.75%) and nairoviruses in 7 (8.75%) pools. All the Rickettsia sequences taken belonged to R. monacensis, while among Borrelia spp., B. garinii and B. valaisiana were detected. Regarding viruses, Norway phlebovirus 1, Sulina and Grotenhout nairoviruses were detected. Although a small number of ticks from only one area in Greece was tested, a variety of pathogens and recently identified viruses were detected, prompting for further studies in ticks and surveillance studies in humans.

The article describes machine learning using artificial neural networks (ANN) to develop the parameters of the friction stir welding (FSW) process for three types of aluminum joints (EN AW 7075). A total of 608 experimental data were used to build the ANN. Two types of networks were built: the first one was used to classify good/bad connections with the MLP 7-19-2 topology, the second one was used to regress the tensile load-bearing capacity with the MLP 7-19-1 topology. Selected parameters of the FSW process were used as input data for ANN training: rotational speed, welding speed, joint and tool geometry. Based on a case study, the quality of the FSW joint was assessed in terms of microstructure and mechanical properties. The usefulness of both trained neural networks has been demonstrated. For the regression network, the quality of the validation set was approximately 93.6%. In the case of confusion matrix for the test set, errors never exceeded 6%. Only 184 epochs were needed to train the regression network. The quality of the validation set was approximately 87.1%. On their basis, predictive maps were developed and presented in the work allowing for the selection of optimal parameters of the FSW process for three types of joints.

Leukemia encompasses diverse blood cancers marked by the unchecked growth and developmental blockage of myeloid or lymphoid precursor cells within the bone marrow and the bloodstream. Treatment traditionally involves intensive chemotherapy or hematopoietic stem cell transplantation, albeit these approaches often yield severe side effects and encounter resistance from leukemia cells. Hence, the quest for novel therapies targeting leukemic cells selectively is paramount to enhancing leukemia outcomes. Exploring natural products is a promising avenue for pursuing effective chemotherapy and preventive measures against leukemia. These compounds serve as a crucial wellspring for drug development and offer a source of biologically active elements for potential therapeutic interventions in leukemia. The enzyme telomerase plays a vital role in stabilizing chromosomes by adding DNA sections to the end of chromosomes during the mitotic cycle. Notably, hTERT mRNA expression strongly correlates with telomerase activity, making it a potential target for therapeutic intervention without adverse systemic effects. Telomerase inhibition is a promising target in cancer therapy, especially in combination with natural ingredients, demonstrating less cytotoxicity than chemotherapies.

Agroforestry systems with multipurpose tree species, for the double production of timber and fruit, along with the intercropping with herbaceous crops, can be fundamental land use systems for multiple ecosystems services. Research was conducted in Italy, for one growing season, in 18 years old agroforestry experimental plots with common walnut (Juglans regia L.) trees, according to the previous agroforestry management (AFM) since tree planting. The aim is i) estimating trees growth and herbaceous yield in relation to increasing tree size, especially referring to the trees basal area (G); ii) evaluating the role of nut production as additional product to timber and food crops. Observations were run on tree growth and leaf phenology, total light transmittance (TTL) below tree canopy and hay yield with and without walnut intercropping. Nut yield was estimated from available literature and associated to G. At the end of the study year, representing two third of trees rotation, their height varied between 10 and 11 m, with much larger variation for tree diameter between 17 and 22 cm, according to the previous AFM, including tree mulching with plastic film. Hay yield varied between 4.5 and 3 Mg ha year-1 outside and under tree canopy, respectively. Negative regression were determined for G and TTL, and G and hay yield, with an annual reduction of 5% per G unit. Simulations were run on hay and nut yield, using G as main input parameter. The work points out the following key findings: i) the usefulness of plastic mulching, at tree planting, to increase the long-term timber productivity of the trees intercropped with herbaceous crops; ii) the negative linear relationship between hay productivity and walnut trees G; iii) G influences, in positive way, more markedly nut than hay yield. Suggestions are given on the optimum tree planting spacing and AFM for balancing timber, nut and hay yields.

Construction Design Management (DM) involves the coordination of design process and activity to ensure quality project design, yet it involves many challenges. This work reports on a collaborative Knowledge Transfer Project (KTP) that tackled several challenges faced by construction design management practitioners. Employing a design science research methodology (DSRM), qualitative analysis of semi-structured interviews with purposively identified design managers first uncovered real world concerns around design coordination, and designer performance monitoring. Then to address these concerns, a web-based design management prototype dashboard was developed which uses typical project data to aid the management of design coordination activity and facilitate design management task prioritization and reporting functionalities. By digitalizing selected design management processes to improve efficiency, and productivity, the visual reporting capability of the dashboard enables design production to be monitored at project and portfolio levels; trends in designer performance to be assessed and focus to be retained on technical queries (TQs) and requests for information (RFIs). By reporting on the development of this web-based Design Management prototype dashboard and illustrating how it can enhance design management productivity in construction firms, this work also provides further evidence of how employing a design science research methodology in construction can produce practical solutions that address real world construction concerns.

: The primary use of the potato is direct consumption. The classification of potatoes into consumption type is made on the basis of consumption evaluation of hydrothermally treated tubers. Nutrient deficiency in the soil, including Mg, contributes to the inhibition of potato growth and development and negatively affects the cooking characteristics of the tubers. Magnesium and biostimulants are responsible for plant nitrogen management. Thus, it is necessary to control the content of harmful nitrates and toxic nitrites in tubers. In potato production, it is also important to maintain appropriate conditions during storage. The purpose of this study was to determine the effect of mineral fertilization with magnesium and a biostimulant preparation during cultivation of edible potato and long-term storage on the traits determining utility-consumption type and the content of harmful nitrogen compounds in tubers. The study was conducted using the early potato variety Satina. In a three-year (2015-2017) three-factor experiment: evaluation date (immediately after harvest and after six months of storage), soil fertilization with mineral magnesium (0, 30, 60, 90 kg MgO ha-1), application of an amino acid biostimulant (0, 1.5, 3.0 l ha-1), five consumption traits of tubers after cooking determining the utility-consumption type of potato were determined. In addition, the consumer safety of the potato was determined based on the nitrate and nitrite content of the tubers. The tendency to overcook tubers significantly depended on all the factors used during potato cultivation. In contrast, the texture and structure of tuber flesh after cooking depended only on mineral fertilization with magnesium. The flesh of the tubers after cooking was most tender (1.0 pt) and firm (1.4 pt) after the application of 60 and 90 kg MgO ha-1, respectively. The moisture level of the flesh after cooking was significantly influenced by the interaction of magnesium fertilization with the biostimulant preparation. The factors of the experiment did not modify the utility-consumption type (B/A), while there was a change in the type in relation to that specified by the grower - general utility (B). The applied cultivation technology and long-term storage did not deteriorate the consumer safety of tubers (&lt;200 mg kg-1) in terms of NO3 (max. 112.1 mg kg-1) and NO2 (max. 1.08 mg kg-1) contents. Organoleptic traits: mealiness, moisture and flesh structure were significantly positively related to nitrate and nitrite content of tubers immediately after harvesting and to nitrite concentration after long-term storage.

Background: Vaccination is one of the most effective life-saving medical interventions, and the introduction of SARS-CoV-2 vaccines was intended to prevent the serious implications of COVID-19. The objectives of the study were: i) to observe the humoral immune response to BNT162b2 vaccine and SARS-CoV-2 infection (mainly breakthrough infections), ii) to demonstrate the persistence of anti-SARS-CoV-2 antibodies over time in relation to the number of received vaccine doses and the course of infection, and iii) to determine adverse effects after primary vaccine doses. Methods: To assess the humoral response, IgG and IgA anti-S1 antibodies were quantified by ELISA assays. In total, the tests were carried out seven times in almost two years. Results: We demonstrated strong immunogenicity (compared to levels before primary vaccination, 150- and 20-fold increases in IgG and IgA, respectively) of the BNT162b2 vaccine. Over time, we observed a systematic decline in antibody levels, which may have contributed to breakthrough infections. Although they caused seroconversion similar to the booster, antibody levels in such patients fell more rapidly than after re-vaccination. On the other hand, in individuals who did not receive booster(s) and who did not present breakthrough infection, anti-SARS-CoV-2 antibodies returned to pre-vaccination levels after 20 months. The most commonly recognized adverse effects were injection site redness and swelling. Conclusion: Vaccination is highly effective in preventing the most severe outcomes of COVID-19 and should be performed regardless of prior infection. Booster doses significantly enhance anti-SARS-CoV-2 antibody levels and, in contrast to those obtained by breakthrough infection, they remain longer.

Mitochondrial dysfunction has been linked to psoriasis, and may be an important underlying factor contributing to this disease. However, a precise methodology for assessing mitochondrial dysfunction has yet to be developed. One promising approach is to measure NADH autofluorescence from affected skin areas. In this study, we show that Flow Mediated Skin Fluorescence (FMSF) can be used for non-invasive assessment of mitochondrial dysfunction in psoriasis. The fluorescence level at baseline and the half-time of ischemic growth (t1/2) derived from the FMSF traces can be used for non-invasive assessment of NADH/NAD+ redox imbalance in psoriatic lesions compared to unaffected skin. These results are supported by analysis of the key FMSF parameters, Reactive Hyperemia Response (RHR) and Hypoxia Sensitivity (HS). This method not only contributes to understanding of the biochemical processes involved in the etiopathogenesis of psoriasis, but also provides a basis for identifying new drug targets and improving the treatment process.

(1) Background: The study presents an assessment of the psychometric properties of the Polish version the Short Schema Mode Inventory (SMI) adaptation. (2) Methods: First, the original version of the scale was translated. Subsequently, a validity and reliability study was conducted on a sample of 240 patients and 400 non-patients. In particular: the factor structure of the inventory was checked, internal reliability and intercorrelations between subscales were tested, differences between the clinical and non-clinical groups in terms of the severity of each factor were examined, and construct validity was assessed by comparing the association of results with external variables. (3) Results: The results did not conclusively confirm the 14-factor structure postulated in the original scale. Nevertheless, relatively the best fit indices were obtained for such a model. Internal reliability for the 14 subscales was in the acceptable to excellent range. Correlations between subscales took moderate to high values. The existence of differences between the clinical and non-clinical groups and the construct validity were confirmed. (4) Conclusions: The psychometric evaluation performed is mostly similar to the results obtained for other adaptations, and the results entitle the inventory to be used for research and clinical purposes with the knowledge of its limitations.

Digital-based differential amplifiers (DDA) are particularly suitable to low voltage digital integrated circuit technologies. This paper presents an exhaustive analysis of the digital-based analog amplifiers to take advantage of today's high-performance digital technologies and of computer-aided design (CAD) commonly employed to design integrated circuits. The operating principle and the main mathematical relations of the digital-based differential amplifiers are discussed along with an exhaustive explanation of its operating regions and of the corresponding power consumption. Finally, a detailed description of the design procedure of in UMC 180nm standard CMOS technology is shown.

In globe artichoke both the edible portion and the waste biomass are recognized as valuable sources of bioactive compounds. For this study, heads with 30 cm-long floral stems, including 2-3 leaves, were harvested from five genotypes which included two traditional vegetatively-propagated varietal types (‘Brindisino,’ and ‘Violetto di Foggia,’) and three "seed"-propagated hybrids (‘Tempo,’ ‘Opal,’ and ‘Madrigal’). The study aimed to determine the total and individual polyphenolic concentrations (measured spectrophotometrically and by HPLC) and antioxidant activity (AA) in different artichoke parts, namely the "hearts" (H), head waste (HW), stem waste (SW), and leaf waste (LW). ‘Brindisino’ SW exhibited the highest accumulation of luteolin (26,317 mg kg−1 F.W.), while ‘Tempo’ H displayed the highest cynarin content (190 mg kg−1 F.W.). ‘Tempo’ HW and H showed the highest levels of apigenin (640 mg kg−1 F.W.), and the greatest source of chlorogenic acid was found in the HW of ‘Opal’ and the H of ‘Brindisino’ (4,300 mg kg−1 F.W.). The hybrids generally exhibited lower total polyphenolic concentrations than the traditional genotypes, particularly evident in the LW. The SW demonstrated the highest concentration of total polyphenols (18,000 mg kg−1 F.W.), followed by the edible H and non-edible HW (12,000 mg kg−1 F.W.), while the LW exhibited the lowest concentration (2,000 mg kg−1 F.W.). Interestingly, the AA did not precisely align with the total polyphenolic concentration, showing slight variations between the examined parts and genotypes.

This article presents a low-cost and flexible solution for acoustic startle response (ASR) tests that can be used in any laboratory with CED Power1401 or a similar Spike2-based interface. ASR is a reflexive response to an unexpected, loud acoustic stimulus, and prepulse inhibition (PPI) is a phenomenon in which the startle response is reduced when preceded by a weak prestimulus of the same modality. Measuring PPI is important because changes in PPI have been observed in patients with various psychiatric and neurological disorders. Commercial ASR testing systems are expensive, and their closed source code affects their transparency and result reproducibility. The proposed setup is easy to install and use, and simultaneous recording of ASR and brain activity (electrocorticogram or intracerebral local field potential) is possible. The Spike2 script is customizable and supports a wide range of PPI protocols. The article presents data obtained in female rats, both wild-type (WT) and dopamine transporter knockout (DAT-KO), showing the same tendency as the data obtained in males, with ASR on single pulse higher than ASR on prepulse+pulse, and PPI reduced in DAT-KO rats compared to WT.

Additive manufacturing (3D printing) and computer-aided design (CAD) still have limited up-take in biomedical and bioengineering research and education, despite the significant potential of these technologies. The utility of organ-scale 3D-printed models of living structures is widely appreciated, while the workflows for microscopy data translation into tactile-accessible replicas are not well developed yet. Here, we demonstrate an accessible and reproducible CAD-based methodology for generating 3D-printed scalable models of human cells cultured in vitro and imaged using conventional scanning confocal microscopy and fused deposition modelling (FDM) 3D printing. We termed this technology CiTo-3DP (Cells-in-Touch for 3D Printing). As a proof-of-concept, we created CiTo-3DP models of human pancreatic cancer cells and healthy dermal fibroblasts by using selectively stained nuclei and the cytoskeleton components (f-actin and α-smooth muscle actin). The production of dismountable sets of cellular components was al-so shown. The CiTo-3DP approach can be adapted to comprehensively present various cell types, subcellular structures and extracellular matrices. We envisage that the resulting CAD and 3D printed models could be used for further applications, including but not limited to in silico simulations for biology, medicine, pharmacological research, tissue engineering, morphometrical analysis, multiphysics modelling, education, rehabilitation of visually impaired people, and integration into virtual reality.

When presenting special relativity, it is customary to single out the so-called paradoxes. One of these paradoxes is the formal occurrence of speeds exceeding the speed of light. An essential part of such paradoxes arises from the incompleteness of the relativistic calculus of velocities. In special relativity, the additive group is used for velocities. However, the use of only group operations imposes artificial restrictions on possible computations. Naive expansion to vector space is usually done by using non-relativistic operations. We propose to consider arithmetic operations in the special theory of relativity in the framework of the Cayley–Klein model for projective spaces. We show that such paradoxes do not arise in the framework of the proposed relativistic extension of algebraic operations.

The tri-trophic interactions between plants, insects, and insect predators and parasitoids are often mediated by chemical cues. The attraction to Herbivore-Infested Plant Volatiles (HIPVs) has been well documented for arthropod predators and parasitoids, and more recently for insectivorous birds. The attraction to plant volatiles induced by the exogenous application of methyl jasmonate (MeJA), a phytohormone typically produced in response to an attack of chewing herbivores, have provided controversial results, both in arthropod and avian predators. In this study, we aimed to examine whether potential differences in the composition of bouquets of volatiles produced by Herbivore-infested and MeJA-treated Pyrenean oak trees (Quercus pyrenaica) were related to differential avian attraction, as results from a previous study suggested. Results showed that the overall emission of volatiles produced by MeJA-treated and Herbivore-infested trees did not differ, and were higher than emissions of Control trees, although MeJA treatment showed more significant reaction and released several specific compounds in contrast to Herbivore-induced trees. These slight differences in the volatile composition may explain why avian predators were not so attracted to MeJA-treated trees as observed in a previous study in this plant-herbivore system. Unfortunately, the lack of avian visits to the experimental trees in the current study does not allow us to confirm this result and points out the need to perform more robust predator studies.

Multiple myeloma (MM) is a genetically complex disease that results from a multistep transformation of normal to malignant plasma cells in the bone marrow. However, the molecular mechanisms responsible for the initiation and heterogeneous evolution of MM remain largely unknown. A fundamental step needed to understand the oncogenesis of MM and its response to therapy is the identification of driver mutations. The introduction of gene expression profiling (GEP) in MM was an important step in elucidating the molecular heterogeneity of MM and its clinical relevance. Since some mutations in myeloma occur in non-coding regions, studies based on the analysis of mRNA provide more comprehensive information on the oncogenic pathways and mechanisms relevant to MM biology. In this review, we discuss the role of gene expression profiling in understanding the biology of multiple myeloma together with the clinical manifestation of the disease, as well as its impact on treatment decisions and future directions.

Age-related macular degeneration (AMD) is a complex, multifactorial neurodegenerative disease that constitutes the most common cause of irreversible blindness in the elderly in developed countries. Incomplete knowledge about its pathogenesis prevents the search for effective methods of prevention and treatment of AMD, primarily its “dry” type, which is by far the most common (90% of all AMD cases). In recent years, AMD became younger: late stages of the disease are now detected in relatively young people. It is known that AMD pathogenesis—according to the age-related structural and functional changes in the retina—is linked with inflammation, hypoxia, oxidative stress, mitochondrial dysfunction, and an impairment of neurotrophic support, but the mechanisms that trigger the conversion of normal age-related changes to the pathological process as well as the reason for early AMD development remain unclear. In the adult mammalian retina, de novo neurogenesis is very limited. Therefore, the structural and functional features that arise during its maturation and formation can exert long-term effects on further ontogenesis of this tissue. The aim of this review is to discuss possible contributions of the changes/disturbances in retinal neurogenesis to the early development of AMD.

A rapid and appropriate genetic and metabolic acclimation, which is crucial for plants’ survival in a changing environment, is maintained due to the coordinated action of plant hormones and cellular degradation mechanisms influencing proteostasis. The plant hormone abscisic acid (ABA) rapidly accumulates in plants in response to environmental stress and plays a pivotal role in the reaction to various stimuli. Increasing evidence demonstrates a significant role of autophagy in controlling ABA signaling. This field has been extensively investigated and new discoveries are constantly being provided. We present updated information on the components of the ABA signaling pathway, particularly on transcription factors modified by different E3 ligases. Then, we focus on the role of selective autophagy in ABA pathway control and review novel evidence on the involvement of autophagy in different parts of the ABA signaling pathway that are important for crosstalk with other hormones, particularly cytokinins and brassinosteroids.

Doxorubicin, a member of the anthracycline family, is a common anticancer agent often used as a first line treatment for the wide spectrum of cancers. Doxorubicin-based chemotherapy, although effective, is associated with serious side effects, such as irreversible cardiotoxicity or nephrotoxicity. Those often life-threatening adverse risks, responsible for the elongation of the patients' recuperation period and increasing medical expenses, have prompted the need for creating novel and safer drug delivery systems. Among many proposed concepts, polymeric nanocarriers are shown to be a promising approach, allowing for controlled and selective drug delivery simultaneously enhancing its activity towards cancerous cells and reducing toxic effects on healthy tissues. This article is a chronological examination of the history of the work progress on polymeric nanostructures, designed as efficient doxorubicin nanocarriers, with the emphasis on the main achievements of 2010-2020. Numerous publications have been reviewed to provide an essential summation of the nanopolymer types and their essential properties, mechanisms towards efficient drug delivery, as well as active targeting stimuli-responsive strategies that are currently utilized in the doxorubicin transportation field.

Biosensors are indispensable tools to follow plant’s immunity as its spatiotemporal dimension is key in withstanding the complex plant immune signaling. The diversity of genetically encoded biosensors in plants is expanding, covering new analytes with ever higher sensitivity and robustness, but their assortment is limited in some aspects, such as their use to follow biotic stress response, employing more than one biosensor in the same chassis and their implementation into crops. In this review, we focused on the available biosensors that encompass these aspects. We show that in vivo imaging of calcium and reactive oxygen species is satisfactorily covered with the available genetically encoded biosensors, while on the other hand they are still underrepresented when it comes to imaging of the main three hormonal players of the immune response, salicylic acid, ethylene and jasmonic acid. Following more than one analyte in the same chassis, upon one or more conditions has so far been possible by using the most advanced genetically encoded biosensors in plants which allow to monitor calcium and two main hormonal pathways involved in plant development, auxin and cytokinin. These kinds of biosensors are also the most evolved in crops. In the last section, we gathered the challenges in the use of the biosensors and showed some strategies to overcome them.

The dynamic development of electromobility poses challenges to designers regarding not only the efficiency of energy transformation but also the battery life, which is influenced by the stability of its operating temperature. Designing cooling systems is connected not only with the optimization of energy management but also with other environmental parameters, such as noise emission. The paper presents the numerical optimization of an innovative radiator for use in electric buses in terms of energy consumption and noise emission. The results of the numerical studies were verified in laboratory and field conditions, showing a very good convergence of the model with the results of the experiments.

Road freight transportation is a key function of modern societies. At the same time, road freight transportation accounts for significant emissions. To reach the UN sustainability goals, sustainable road freight transportation is key. Digitalization, including automation, digitized information, and AI provide opportunities to improve efficiency, reduce costs, and increase service levels in road freight transportation. Digitalization may also radically change the business ecosystem in the sector. In this paper, the question “How will digitalization change the road freight transportation landscape?” is addressed by developing four different future scenarios, using Sweden as a case study. For each of the four scenarios the impacts on the road freight transportation sector are investigated, and opportunities and barriers to reach a sustainable transportation system in each of the scenarios are discussed. In all scenarios an increase in vehicle kilometers travelled is predicted, and in three of the four scenarios significant increases of recycling and urban freight flows are predicted. The scenario development process highlighted how there are important uncertainties in the development of the society that will be highly important for the development of the digitized freight transportation landscape. One example is the sustainability paradigm, which was identified as a strategic uncertainty.

This work studies a novel low voltage (<60 V) hybrid system that supports engine boosting and downsizing in addition to start-stop, regenerative braking, and constrained torque assist/regeneration. The hybrid power split supercharger (PSS) shares a 9 kW motor between supercharging the engine or providing hybrid functionalities through a planetary gear set, a brake and a bypass valve. The PSS operation is limited to only one of the parallel hybrid or boosting modes at a time, necessitating a highly optimized decision making algorithm to select the device mode and power split ratio. In this work an adaptive equivalent consumption minimization strategy (A-ECMS) is developed for energy management of the PSS. The A-ECMS effectiveness is compared against a dynamic programming (DP) solution with full drive cycle preview through hardware-in-the-loop experiments on an engine dynamometer testbed. The experiments show that the PSS with A-ECMS reduces a vehicle fuel consumption by 18.4% over standard FTP75 cycle compared to a baseline turbocharged engine, while global optimal DP solution decreases the fuel consumption by 22.8% compared to baseline.