Lee, S.; Kim, B.W.; Shin, H.-S.; Go, A.; Lee, M.-H.; Lee, D.-K.; Kim, S.; Jeong, O.C. Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis. Micromachines2019, 10, 770.
Lee, S.; Kim, B.W.; Shin, H.-S.; Go, A.; Lee, M.-H.; Lee, D.-K.; Kim, S.; Jeong, O.C. Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis. Micromachines 2019, 10, 770.
Lee, S.; Kim, B.W.; Shin, H.-S.; Go, A.; Lee, M.-H.; Lee, D.-K.; Kim, S.; Jeong, O.C. Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis. Micromachines2019, 10, 770.
Lee, S.; Kim, B.W.; Shin, H.-S.; Go, A.; Lee, M.-H.; Lee, D.-K.; Kim, S.; Jeong, O.C. Aptamer Affinity-Bead Mediated Capture and Displacement of Gram-Negative Bacteria Using Acoustophoresis. Micromachines 2019, 10, 770.
Abstract
Here, we report a simple and effective method for separation of gram-negative bacteria using aptamer-modified microbeads and acoustophoresis. As acoustophoresis allows for simultaneous washing and size-dependent separation in continuous flow mode, we efficiently obtained gram-negative bacteria that showed high affinity without any additional washing steps. The proposed device has a simple and efficient channel design, utilizing a long, square-shaped microchannel that shows excellent separation performance in terms of the purity, recovery, and concentration factor. Microbeads (10 µm) coated with the GN6 aptamer can specifically bind gram-negative bacteria. Using acoustophoresis, gram-negative bacteria-bound microbeads and other unbound/contaminants can be separated by size with high purity and recovery. The device demonstrated excellent separation performance, with high recovery (up to 98%), high purity (up to 99%), and a high volume rate (500 µL/min), and a concentration factor of up to 20×. The acoustophoresis microfluidic device also showed binding affinity to multiple strains of gram-negative bacteria, but not to gram-positive bacteria. This study presents a new paradigm for early diagnosis of bacterial infectious diseases. In addition to detecting living bacteria or bacteria-derived biomarkers, this protocol can be extended to monitoring the contamination of water resources, and may aid quick responses to bioterrorism and pathogenic bacterial infections.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
