Pelevin, A.; Kurzina, N.; Zavialov, V.; Volnova, A. A Custom Solution for Acoustic Startle Response Setup with Spike2-Based Data Acquisition Interface. Methods Protoc.2023, 6, 57.
Pelevin, A.; Kurzina, N.; Zavialov, V.; Volnova, A. A Custom Solution for Acoustic Startle Response Setup with Spike2-Based Data Acquisition Interface. Methods Protoc. 2023, 6, 57.
Pelevin, A.; Kurzina, N.; Zavialov, V.; Volnova, A. A Custom Solution for Acoustic Startle Response Setup with Spike2-Based Data Acquisition Interface. Methods Protoc.2023, 6, 57.
Pelevin, A.; Kurzina, N.; Zavialov, V.; Volnova, A. A Custom Solution for Acoustic Startle Response Setup with Spike2-Based Data Acquisition Interface. Methods Protoc. 2023, 6, 57.
Abstract
This article presents a low-cost and flexible solution for acoustic startle response (ASR) tests that can be used in any laboratory with CED Power1401 or a similar Spike2-based interface. ASR is a reflexive response to an unexpected, loud acoustic stimulus, and prepulse inhibition (PPI) is a phenomenon in which the startle response is reduced when preceded by a weak prestimulus of the same modality. Measuring PPI is important because changes in PPI have been observed in patients with various psychiatric and neurological disorders. Commercial ASR testing systems are expensive, and their closed source code affects their transparency and result reproducibility. The proposed setup is easy to install and use, and simultaneous recording of ASR and brain activity (electrocorticogram or intracerebral local field potential) is possible. The Spike2 script is customizable and supports a wide range of PPI protocols. The article presents data obtained in female rats, both wild-type (WT) and dopamine transporter knockout (DAT-KO), showing the same tendency as the data obtained in males, with ASR on single pulse higher than ASR on prepulse+pulse, and PPI reduced in DAT-KO rats compared to WT.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
