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Preprint ARTICLE | doi:10.20944/preprints202404.1845.v1

Determination Mitochondria's Quality Control of Parkinsonism Using Real-Time Reverse-Transcription Polymerase Chain Reaction

Al-Baraa Akram

Subject: Neuroscience And Neurology, Biology And Life Sciences Keywords: RT: real time, PCR: polymerase chain reaction, WT: wild type, dNTP: deoxynucleotide triphosphate

Online: 28 April 2024 (10:36:25 CEST)

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Nuclear DNA mutations, mitochondrial DNA mutations, combined nuclear and mitochondrial DNA defects, and random occurrences are the main causes of mitochondrial disease. Black pigmentation loss is a major sign of Parkinsonism. Because of the loss of dopaminergic neurons in the substantia nigra, it drops to less than 80%. The degree of motor impairment was correlated with the amount of black pigmentation loss. After RNA extraction, reverse transcription is carried out because reverse transcriptase was used to create cDNA. One microliter of total RNA supplemented with IORT Buffer, dNTPs, random hexamers, Multi-Scribe Reverse Transcriptase, and nuclease-free was used to create cDNA. The following was how the reaction was carried out in a thermal cycler: 10 minutes at 25°C, 120 minutes at 37°C, 5 seconds at 85°C, and lastly 4°C. The data was expressed as a v-value, which shows how much the target gene is enriched in comparison to the reference gene. Earlier research using mice injected with functional HtrA2/Omi protease activity revealed similar outcomes, including induced up-regulation of CHOP, decreased mitochondrial function, and early brain cell aging because of an increase in mtDNA deletions. The cell viability of the genotypes exhibits a dmg concentration-dependent effect in these results, suggesting that high content ratios of drug treatment with 6-OHDA may be the cause of cellular content depletion. After 6-OHDA neurotoxin treatment, CHOP is up-regulated. Prior in vitro research has shown that treatment with a model based on 6-OHDA neurotoxin causes the activation of mitochondrial stress pathways, which in turn promotes the death of neurons. 6-OHDA reversibly inhibited complexes I and IV's activities in rat brain cells, which led to dopaminergic neurodegeneration, a defining feature of PD pathogens. As a result, the number of living cells will directly correlate with the dmg concentration, supporting the results of the current study. Real-time PCR can generally demonstrate that the removal of HtrA 2 results in a significant up-regulation of Hsp60 expression when compared to WT cells. This could be linked to a decreased threshold for mitochondrial stress in HtrA2 KO cells, which would explain why, following ADEP4 and ACP5 treatment, Hsp60 is significantly up-regulated in HtrA2 KO cells compared to WT cells.

Preprint ARTICLE | doi:10.20944/preprints201801.0221.v1

Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus

Yuan Hui, Zhiming Wu, Zhiran Qin, Li Zhu, Junhe Liang, Xujuan Li, Hanmin Fu, Shiyu Feng, Weiwei Xiao, Qinghua Wu, Bao Zhang, Wei Zhao

Subject: Biology And Life Sciences, Virology Keywords: Zika virus; nucleic acid detection; micro-droplet digital polymerase chain reaction; real-time fluorescence quantitative polymerase chain reaction

Online: 24 January 2018 (04:21:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202208.0392.v1

Comparison of Four Real-time Polymerase Chain Reaction Assays for the Detection of SARS-CoV-2 on respiratory samples from Tunja, Boyacá - Colombia

Lorenzo Hernando Salamanca Neita, Óscar Leonardo Carvajal Rojas, Juan Pablo Carvajal Rojas, Ruth Maribel Forero, Niya Alexandra Segura

Subject: Biology And Life Sciences, Virology Keywords: Severe Acute Respiratory Syndrome-CoV-2 (SARS-CoV2), COVID-19, molecular diagnostics, real-time polymerase chain reaction (RT-qPCR)

Online: 23 August 2022 (03:55:44 CEST)

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Background: Coronavirus disease (COVID-19) is an infectious disease caused by the SARS-CoV-2. In Colombia, many commercial methods are now available to perform the RT-qPCR assays, and the laboratories must evaluate its diagnostic accuracy to ensure reliable results to suspected COVID-19 patients. The purpose of the study was to compare four commercial RT-qPCR assays for detection of SARS-CoV2 virus, from nasopharyngeal swab samples referred to Laboratorio Carvajal IPS, SAS of Tunja, Boyacá - Colombia. Methods: This prospective study was conducted on 152 samples of respiratory tract samples (Nasopharyngeal Swab) from patients with suspected SARS-CoV-2 infection. Diagnostic accuracy of GeneFinderTM COVID-19 Plus RealAmp (In Vitro diagnostic), One-Step Real-Time RT-PCR (Vitro Master diagnostica), Berlin modified protocol and gold standard Berlin protocol (Berlin Charite Probe One-Step RT-qPCR Kit, New England Biolabs) as reference was assessed. Operational characteristics were estimated in terms of sensitivity, specificity, agreement, and predictive values. Results: Using Berlin Charite Probe One-Step RT-qPCR Kit as reference, the sensitivity/specificity for the diagnostic tests were found to be GeneFinderTM COVID-19 Plus RealAmp Kit 100%/92.7%, One-Step Real-Time RT-PCR, One-Step Real-Time RT-PCR 92.75%/67.47%, and Berlin modified protocol 100%/96.39%. The results of four commercially available methods were found to be consistent with those obtained from Berlin Modified protocol analysis for % of the samples and showed good agreement (κ= 0.96). Concordant SARS-CoV2 negative and positive RT-qPCR results were reported for xxx and xxx samples, respectively. Summarize something about the Ct. Conclusion: Our data demonstrate that all commercially available methods are rapid and reliable for the identification of SARS-CoV-2 virus associated with COVID-19. One-Step RT-qPCR Kit and GeneFinderTM COVID-19 Plus RealAmp assay show optimal sensitivity compared with Belin modified protocol. In addition, there is no significant correlation between xxxxx

Preprint ARTICLE | doi:10.20944/preprints201703.0090.v1

Design and Imaging of Ground-Based Multiple-Input Multiple-Output Synthetic Aperture Radar (MIMO SAR) with Non-Collinear Arrays

Cheng Hu, Jingyang Wang, Weiming Tian, Tao Zeng, Rui Wang

Subject: Engineering, Electrical And Electronic Engineering Keywords: MIMO radar; MIMO imaging; Near-field imaging; Height difference between T/R arrays; Grating lobes

Online: 15 March 2017 (00:48:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1094.v1

Development and Evaluation of an In-House Real-Time Rt-Pcr Targeting nsp10 Gene for SARS-CoV-2 Detection

Cyril Chik-Yan Yip, Jane Hau-Ching Poon, Kit-Hang Leung, Wan-Mui Chan, Jonathan Daniel Ip, Allen Wing-Ho Chu, Vincent Chi-Chung Cheng, Kwok-Yung Yuen, Kelvin Kai-Wang To

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; nsp10; real-time RT-PCR; diagnostic

Online: 20 February 2024 (06:07:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1440.v1

Development of an Accurate Polymerase Chain Reaction (PCR) Assay for Genetic Sex Identification in Lumpfish (Cyclopterus lumpus) based on Male-specific anti-Mullerian Hormone (amh) Gene

Kasidis Chaiyasut, Paul Merviel, Peter Palma, Johanna Perschthaler, Eduardo Jimenez Fernandez, Andrew Davie, Alejandro P. Gutierrez

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Anti-Müllerian hormone; Cyclopterus lumpus; Male-specific marker; Genetic sex identification

Online: 19 May 2023 (11:36:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202109.0421.v1

A Practical Approach for Quantitative Polymerase Chain Reaction, the Gold Standard in Microbiological Diagnosis

Tímea Mosolygó, Krisztián Laczi, Gabriella Spengler, Katalin Burián

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: laboratory practical class; undergraduate teaching; qPCR

Online: 24 September 2021 (09:04:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202106.0285.v1

Quantitative Fluorescent Polymerase Chain Reaction for Prenatal Diagnosis and Its Application in Vietnam: A Literature Review

Thuan Phan

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: QF-PCR; cytogenetics; prenatal diagnosis; aneuploidy; chromosome abnormalities; screening test

Online: 10 June 2021 (09:17:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201704.0154.v1

Water Bridging Dynamics of Polymerase Chain Reaction in the Gauge Theory Paradigm of Quantum Fields

L. Montagnier, J. Aïssa, A. Capolupo, T. J. A. Craddock, P. Kurian, C. Lavallee, A. Polcari, P. Romano, A. Tedeschi, G. Vitiello

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: water bridging; dipole waves; coherent states; polymerase chain reaction; DNA amplification; DNA transduction; enzyme catalytic activity; fractal-like self-similarity

Online: 25 April 2017 (04:24:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0993.v1

Mycobacterium avium subsp. paratuberculosis in Wild Boar (Sus scrofa) in Portugal

Ana Cristina Matos, Luís Figueira, Maria Helena Martins, Luís Cardoso, Manuela Matos, Maria de Lurdes Pinto, Ana Cláudia Coelho

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Epidemiology; Mycobacterium avium subsp. paratuberculosis; ; wild boar

Online: 16 April 2024 (07:26:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0581.v1

Looking into the Quantification of Forensic Samples with Real-Time PCR

Ugo Ricci, Dario Ciappi, Ilaria Carboni, Claudia Centrone, Irene Giotti, Martina Petti, Alice Brogi, Elisabetta Pelo

Subject: Biology And Biotechnology, Biology And Life Sciences Keywords: DNA amplification; polymerase chain reaction; DNA quantification; forensic DNA

Online: 9 May 2024 (10:39:14 CEST)

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The quantification of human DNA extracts from forensic samples plays a key role in the forensic genetics process. Quantification guarantees maximum efficiency and avoids repeated analyses, over amplified samples, or unnecessary examinations. Here, we present our experience using an extremely sensitive real-time-based method with the Quantifiler® Trio DNA quantification kit, which we have explored in the ISO/IEC17025 accredited test validation process conducted in our laboratory. In this study, our focus was on establishing a minimum threshold beyond which DNA typing is unnecessary, by defining the correlation of negative quantification results with the absence of genetic profiles resulting from DNA typing. We investigated the results in the range in which the technique is used (50-0.005 ng/μl). In the validation process of the internally accredited method, we used data from the commercial quantification system, based on the average values of the standard curve using a wide range of samples, including artificially degraded samples. This method was extensively used continuously for nine years to quantify the DNA extract coming from a wide range of samples from criminal cases. The validation allowed us to verify the limits of the technique, especially in the use of the minimum quantities necessary to obtain genetic profiles acceptable for sending to the Italian DNA database. This optimization of the protocol for analysing forensic samples allows us to optimise the protocol for analysing forensic samples. However, the technique is used in our laboratory with a completely manual preparative phase, and we have decided to investigate the contribution of human error more accurately. Thus, in our experience, we have found that it is preferable to use quantification ranges rather than exact limits before deciding how to analyse the extracts via PCR. In addition, here we present some preliminary data related to the analysis of certain samples that initially appeared negative during quantification, applying a concentration method for maximum yield from forensic samples of judicial interest. Our aim is to ensure that no source of evidence is lost in the reconstruction of a criminal event.

Preprint ARTICLE | doi:10.20944/preprints202309.1546.v1

Real-Time Extension of TAO-DFT

Hung-Yi Tsai, Jeng-Da Chai

Subject: Physical Sciences, Chemical Physics Keywords: RT-TAO-DFT; TAO-DFT; multi-reference character; real-time electron dynamics; time-dependent properties; high-order harmonic generation

Online: 22 September 2023 (11:40:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.2102.v1

Real-Time Classification of Motor Imagery using Dynamic Window-level Granger Causality Analysis of fMRI Data

Tianyuan Liu, Bao L, Chi Zhang, Panpan Chen, WeiChen Zhao, Bin Yan

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: BCI, rt-fMRI, MI, DWGC, svm

Online: 31 August 2023 (09:43:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.2136.v1

Multiplex Real-Time RT-PCR Assays for Detection and Differentiation of Porcine Enteric Coronaviruses

Christina M. Lazov, Alice Papetti, Graham J. Belsham, Anette Bøtner, Thomas Bruun Rasmussen, Maria Beatrice Boniotti

Subject: Biology And Life Sciences, Virology Keywords: Virus diagnosis; RNA; recombinant virus; alphacoronavirus; deltacoronavirus; porcine epidemic diarrhea virus (PEDV); transmissible gastroenteritis virus (TGEV); swine enteric coronavirus (SeCoV); porcine deltacoronavirus (PDCoV)

Online: 29 June 2023 (13:22:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202401.1764.v1

Real-Time Performance Benchmarking of RISC-V Architecture: Implementation and Verification on an EtherCAT-Based Robotic Control System

Taeho Yoo, Byoung Wook Choi

Subject: Engineering, Control And Systems Engineering Keywords: RISC-V; FreeRTOS; PREEMPT-RT; RTOS; EtherCAT; Embedded Linux

Online: 24 January 2024 (14:43:54 CET)

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RISC-V offers a modular technical approach combined with an open, royalty-free instruction set architecture (ISA). This openness allows various researches to incorporate RISC-V into both open and proprietary solutions or services. However, despite its advantages as a fundamental building block for many embedded systems, the escalating complexity and functional demands of real-time applications have made adhering to response time deadlines challenging. This has led to an increased demand for comprehensive development insights for real-time systems based on RISC-V. To improve development efficiency, we offer a detailed performance analysis of RISC-V in comparison to various ISAs. In this paper, we analyze the real-time performance of RISC-V through two real-time approaches based on processor architectures. For RTOS applications, we adopted FreeRTOS and evaluated its performance on HiFive1 Rev B (RISC-V) and STM3240G-EVAL (ARM M) to evaluate real-time performance. For Real-time Linux, we utilized Linux with the Preempt-RT patch and tested its performance on VisionFive 2 (RISC-V), MIO5272 (x86-64), and Raspberry Pi 4 B (ARM A). Through these experiments, we examined the real-time mechanism of each operating system. Additionally, in the Preempt-RT experiments, scheduling latencies were evaluated using cyclictest. Finally, in order to practically show the real-time capabilities of RISC-V, we implemented motion control of a six-axis collaborative robot, which was performed on the VisionFive 2 platform. This implementation provided a comparative result of RISC-V’s performance against the x86-64 architecture of MIO-5272. Ultimately, the results indicated that the real-time performance of RISC-V for real-time applications was slightly inferior to that of the x86-64 architecture. A noticeable achievement of this research was the first implementation of an EtherCAT Master on RISC-V designed for real-time applications featuring a multi-tasking structure. The research offers valuable insights into the real-time capabilities of RISC-V compared to established architectures like x86-64. While RISC-V might not surpass these architectures in real-time performance, its flexibility, modularity, and open nature make it a compelling choice for a wide range of real-time applications. The successful implementation of the EtherCAT Master on RISC-V further expands its potential in the domain of real-time embedded control systems.

Preprint ARTICLE | doi:10.20944/preprints202401.0481.v1

Development of a Real-Time PCR Assay for Early Detection of the Eucalyptus Pathogen Quambalaria eucalypti

Roberto Faedda, Gabriela B. Silva

Subject: Biology And Life Sciences, Life Sciences Keywords: Quambalaria eucalypti; Quambalaria shoot blight; qPCR; diagnostics; molecular detection; Eucalyptus.

Online: 5 January 2024 (10:13:13 CET)

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Working Paper ARTICLE

Detection of Peanut Allergen by Real Time PCR: Looking For the Suitable Detection Marker as Affected by Processing

Africa Sanchiz, Paulina Sánchez-Enciso, Carmen Cuadrado, Rosario Linacero

Subject: Biology And Life Sciences, Anatomy And Physiology Keywords: Real-time PCR; peanut; food allergen; chloroplast marker; DNA isolation

Online: 16 June 2021 (11:33:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202102.0525.v1

Exploring New Scaffolds for the Dual Inhibition of HIV-1 Rt Polymerase and Ribonuclease Associated Functions

Rita Meleddu, Angela Corona, Simona Distinto, Filippo Cottiglia, Serenella Deplano, Lisa Sequeira, Daniela Secci, Alessia Onali, Erica Sanna, Francesca Esposito, Italo Cirone, Francesco Ortuso, Stefano Alcaro, Enzo Tramontano, Peter Matyus, Elias Maccioni

Subject: Chemistry And Materials Science, Analytical Chemistry Keywords: HIV-RT; ribonuclease H; dual inhibitors; docking; putative binding

Online: 23 February 2021 (15:51:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1920.v1

Selection and Evaluation of Reference Genes for qRT-PCR Analysis in Amorphophallus konjac Based on Transcriptome Data

Yanli Liu, Chengcheng Zhang, Nunung Harijati, Ying Diao, Erxi Liu, Zhongli Hu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Amorphophallus; reference genes; RT-qPCR; gene expression

Online: 27 June 2023 (13:43:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0076.v2

Effect of Instant Controlled Pressure Drop (DIC) Treatment on the Detection of Nut Allergens by Real Time PCR

Africa Sanchiz, Carmen Cuadrado, Joseph Haddad, Rosario Linacero

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Real time PCR; tree nuts; allergen detection; processed foods; thermal processing; pressure processing; DIC processing

Online: 16 May 2020 (19:18:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202005.0440.v1

Clinical Performance Analysis and Evaluation of Quantitative Real Time PCR for Diagnosis of Scrub Typhus in North East India

Diptanu Paul, W Valarie Lyngdoh, Himesh Barman, Clarissa Jane Lyngdoh, Kryshanlang Lynrah, Elantamilan Durairaj

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Scrub typhus; qRT PCR; Quantitative PCR; real time PCR; IgM ELISA; North-East India

Online: 27 May 2020 (07:51:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201807.0490.v1

Display In the Wild (DIW): An Adaptive Projection-Imaging System to Screen Geometry in Real Time

Byungyong Ryu, Sung-Ho Bae

Subject: Engineering, Control And Systems Engineering Keywords: TV, monitor, projector distortion, distortion correction, calibration, depth camera, Unmanned Aerial Vehicles, UAVs, display.

Online: 25 July 2018 (15:36:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0994.v1

Design of Unmanned Road Width in Open-Pit Mines Based on Offset Reaction Time

Liu Han, Peng Liu

Subject: Engineering, Mining And Mineral Processing Keywords: Unmanned driving; Truck dynamics; Offset reaction time; Road width; Economic benefits

Online: 18 March 2024 (08:09:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1075.v2

Towards Real-Time Railroad Inspection Using Directional Eddy Current Probe

Meirbek Mussatayev, Mohammed Alanesi

Subject: Engineering, Transportation Science And Technology Keywords: Eddy current testing; lift-off; real-time inspection; probe optimization; signal-to-noise ratio

Online: 15 April 2024 (14:40:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202006.0284.v1

Factors Affecting SARS-CoV-2 (COVID-19) Pandemic, including Zoonotic, Human Transmission and Chain of Infection. Reducing Public Health Risk by Serum Antibody Testing, Avoiding Screening in Unhygienic Places and False PCR Reporting. A Scientific Review

Kamran Mahmood Ahmed Aziz, Abdullah Othman, Waleed Alqahtani, Sumaiya Azhar

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; RT-PCR; antibody; zoonotic; animal transmission; genomics; asymptomatic fraction; herd immunity

Online: 23 June 2020 (13:30:11 CEST)

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Since December 2019, a rapid increase in the number of SARS-CoV-2 (COVID-19) cases was reported worldwide, despite strict infection control and lock down measures. Current paper investigated the actual facts behind this rapid increase in the number of cases. Study of genomic sequence reveals that domestic and wild animals were likely ancestors and zoonotic source for SARS-CoVs, MERS-CoVs, and SARS-CoV-2. Strong evidence suggest that these viruses already existed and replicated in animals and humans during past several decades, exhibiting diverse mutations, evolutions and self-limiting diseases, except during outbreaks. Serious zoonotic reservoir investigations are required to investigate animal transmission of SARS-CoVs and SARS-CoV-2 to limit current pandemic. This might be the reason of increasing number of cases via animals. SARS-CoV-2 has been retrospectively isolated in different studies in August 2019, several months before Wuhan announced. Hence, there is a possibility that viruses existed, went undetected, infecting subclinically, in past several years, and SARS-CoV-2 antigens and neutralizing antibodies may have been present in humans since long time. This might be another reason of increasing number of cases by screening as mass screening and antigen or antibody testing was not carried out in the past years. Randomized controlled trials are required to investigate human to human transmission by touch, as the current evidence is limited with conflicting results. As all SARS-CoVs are basically respiratory viruses, droplet precautions and infection control measures are essential, especially for hospital staff. Increased number of SARS-CoV-2 asymptomatic, or subclinical cases are detected worldwide. This silent phase of transmission can be beneficial for humans. Lack of symptoms eventually lessen virus transmission and reduce the pathogen's long-term survival and provide humoral herd immunity up to several years. Hence, seropositivity with diverse antibodies develops against mutating SARS-CoVs which will confer strong immunity during epidemics. Strategies such as identification, contact tracing and quarantine are costly and practically difficult. Hence, asymptomatic persons can continue their work with droplet precautions and standard infection control procedures, while symptomatic or sick persons can isolate themselves in their homes without the need for strict quarantine until clinical recovery, with reduced hospital visits and minimizing chances of hospital acquired infections. RT-PCR has low sensitivity and specificity, carries a high risk of handling live virus antigens, and requires difficult protocols. As viral load also sharply declines after few days of onset of infection, this technique might overlook infection. Furthermore, SARS-CoV-2 infection may be present in blood when oropharyngeal swabs are negative by RT-PCR. Additionally, RT-PCR usually gives false negative and false positive results and must be interpreted cautiously. This might be again a reason of increasing number of cases by false positive RT-PCR reporting. Moreover, antibodies against SARS-CoVs develop robustly in serum even by reduced amount of antigens. In contrast to RT-PCR, ELISA for diagnosing antibodies against SARS-CoV-2 demonstrates 100% specificity and 100% sensitivity, even in clinically asymptomatic individuals. These antibodies can be used for serologic surveys, monitoring and screening. However, screening tests for SARS-COV-2 should be avoided in unhygienic public places by nasopharyngeal swabs, which carry a high risk of further transmission, co-infection or superinfection. Such highly infectious virus must be isolated and tested in highly sterilized laboratory. Further strict international laws and policies are required to stop the possible spread of experimental viruses, biological warfare and bioterrorism.

Preprint COMMUNICATION | doi:10.20944/preprints202306.2007.v1

Ct-Value from qRT-PCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success

Dominik Hadzega, Klaudia Babisova, Michaela Hyblova, Nikola Janostiakova, Peter Sabaka, Pavol Janega, Gabriel Minárik

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; RNA genome assembly; RT-qPCR; SPAdes

Online: 29 June 2023 (02:12:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.0958.v1

Logistics Center Selection and Logistics Network Construction from the Perspective of Urban Geographic Information Fusion

Zhanxin Ma, Xiyu Zheng, Hejun Liang, Ping Luo

Subject: Business, Economics And Management, Business And Management Keywords: Last mile; Logistics nodes; Clustering analysis; Greedy algorithm

Online: 19 February 2024 (11:19:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints201810.0594.v2

User Satisfaction in Augmented Reality-Based Training Using Microsoft HoloLens

Hui Xue, Puneet Sharma, Fridolin Wild

Subject: Computer Science And Mathematics, Computer Science Keywords: augmented reality; Microsoft HoloLens; AR application; user experience; user satisfaction

Online: 16 November 2018 (07:55:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1308.v1

Quality Changes Monitoring of Fresh-Cut Apples during Distribution in Real-Time Cold Chain Systems Application

Andri Jaya Laksana, Jong-Hoon Kim, Jae-Hwan An, Ji-Young Kim

Subject: Biology And Life Sciences, Other Keywords: Fresh-cut apple; temperature; quality changes; storage; cold chain systems

Online: 18 December 2023 (15:04:56 CET)

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Preprint ARTICLE | doi:10.20944/preprints202404.1215.v1

Evaluating the VIIRS Imagery for Developing Near Real-Time Nationwide Vegetation Cover Monitoring in Indonesia

Yudi Setiawan, Kustiyo Kustiyo, Sahid Agustian Hudjimartsu, Judin Purwanto, Riva Rovani, Anna Tosiani, Basyiruddin Usman, Tatik Kartika, Novie Indriasari, Lilik Budi Prasetyo, Belinda Arunarwati Margono

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: change detection; temporal vegetation dynamics; open area index; multi temporal VIIRS

Online: 18 April 2024 (10:57:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201906.0144.v1

Applications of Data Mining Algorithms for Network Security

Kai Chain

Subject: Computer Science And Mathematics, Security Systems Keywords: data mining; network security; association rules; DDoS

Online: 16 June 2019 (02:42:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201703.0107.v1

Real-Time Forecasting of EV Charging Station Scheduling for Smart Energy Systems

Bharatiraja Chokkalingam, Sanjeevikumar Padmanaban, Pierluigi Siano, Ramesh Krishnamoorthy, Raghu Selvaraj

Subject: Engineering, Electrical And Electronic Engineering Keywords: electric vehicle (EV); charging station (CS); state of charge (SOC); structured query language (SQL); personal home page (PHP)

Online: 16 March 2017 (06:36:11 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0184.v1

Non-Covalent Interactions between dUTP C5-Substituents and DNA Polymerase Result in a Decrease in PCR Efficiency

Olga A. Zasedateleva, Sergey A. Surzhikov, Viktoriya E. Kuznetsova, Valeriy E. Shershov, Victor E. Barsky, Alexander S. Zasedatelev, Alexander V. Chudinov

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: C5-modified dUTPs; A and B family DNA polymerases; PCR amplification; X-ray structure; molecular modeling; non-covalent interactions

Online: 2 August 2023 (08:21:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0091.v1

Evidence-Based Evaluation of PCR Diagnostics for SARS-Cov-2 and The Omicron Variants by Sanger Sequencing

Sin Hang Lee

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: SARS-CoV-2; SARS-CoV; RT-PCR; Sanger sequencing; RT-qPCR; receptor-binding domain (RBD); N-terminal domain (NTD); Omicron; multi-allelic SNPs; false-positive

Online: 12 April 2022 (03:57:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201901.0212.v1

One-tube RT-PCR for the Simultaneous Detection and Typing Duck Hepatitis A Virus Subtypes 1 and 3

Xueming Chen, Yuhuan Chen, Chunguo Liu, Xiaojun Li, Hongyu Liu, Xiaofei Bai, Ming Liu, Yun Zhang

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: DHAV-1; DHAV-3; Phylogenetic analysis; One-tube RT-PCR; Simultaneously

Online: 22 January 2019 (11:08:19 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0224.v1

Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods

Emmanuel Marin, Fernando Messina, Mercedes Romero, Alicia Arechavala, Roxana Depardo, Gabriela Santiso

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: histoplasmosis; Histoplasma antigen; Histoplasma diagnosis; Blood culture; Latin America.

Online: 4 May 2023 (08:18:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.1005.v1

First Immunohistochemical Demonstration of the Expression of a Type-2 Vomeronasal Receptor, V2R, in Wild Canids

Irene Ortiz-Leal, Mateo V. Torres, Ana María López-Beceiro, Luis Fidalgo, Taekyun Shin, Roberto Tirindelli, Pablo Sanchez-Quinteiro

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: vomeronasal organ; V2R; G-proteins; Canidae; immunohistochemistry

Online: 18 March 2024 (10:48:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0337.v1

Screening and Validation of Internal Reference Genes for Quan-Titative Real-Time PCR Analysis of Leaf Color Mutants in Den-Drobium Officinale

Hua Cao, Han Li, Lin Lu, Yulu Ji, Lulin Ma, Shenchong Li

Subject: Biology And Life Sciences, Plant Sciences Keywords: Dendrobium officinale; leaf color; qRT-PCR; reference gene; leaf color mutant

Online: 5 May 2023 (09:34:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0220.v1

Selection and Multiplexing of Reverse Transcription Quantitative PCR Tests Targeting Relevant Honeybee Viral Pathogens

Franca Rossi, Ilaria Del Matto, Luciano Ricchiuti, Lucio Marino

Subject: Life Sciences, Biology And Life Sciences Keywords: ; honeybee; viral pathogens; RT-qPCR tests; TaqMan probes; duplex reactions ;

Online: 6 May 2024 (08:43:43 CEST)

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Working Paper BRIEF REPORT

Positive Rate Is Useful to Evaluate the Sufficiency of RT-PCR Test Availability for COVID-19

Sadao Suzuki

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; RT-PCR test; pre-test probability; positive rate

Online: 5 May 2020 (06:04:41 CEST)

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Preprint REVIEW | doi:10.20944/preprints202104.0481.v1

Minimizing Errors in RT-PCR Detection and Quantification of SARS-CoV-2 RNA for Wastewater Surveillance

Warish Ahmed, Stuart Simpson, Paul Bertsch, Kyle Bibby, Aaron Bivins, Linda Blackall, Silvia Bofill-Mas, Albert Bosch, Joao Brandao, Phil Choi, Mark Ciesielski, Erica Donner, Nishita D'Souza, Andreas Farnleitner, Daniel Gerrity, Raul Gonzalez, John Griffith, Pradip Gyawali, Charles Haas, Kerry Hamilton, Chanditha Hapuarachchi, Valerie Harwood, Rehnuma Haque, Greg Jackson, Stuart Khan, Wesaal Khan, Masaaki Kitajima, Asja Korajkic, Giuseppina La Rosa, Blythe Layton, Erin Lipp, Sandra McLellan, Brian McMinn, Gertjan Medema, Suzanne Metcalfe, Wim Meijer, Jochen Mueller, Heather Murphy, Colleen Naughton, Rachel Noble, Sudhi Payyappat, Susan Petterson, Tarja Pitkanen, Veronica Rajal, Brandon Reyneke, Fernando Roman, Joan Rose, Marta Rusinol, Michael Sadowsky, Laura Sala-Comorera, Yin Xiang Setoh, Samendra Sherchan, Kwanrawee Sirikanchana, Wendy Smith, Joshua Steele, Rosalie Sabburg, Erin Symonds, Phong Thai, Kevin Thomas, Josh Tynan, Simon Toze, Janelle Thompson, Andy Whiteley, Judith Wong, Daisuke Sano, Stefan Wuertz, Irene Xagoraraki, Qian Zhang, Amity Zimmer-Faust, Orin Shanks

Subject: Environmental And Earth Sciences, Waste Management And Disposal Keywords: COVID-19; SARS-CoV-2; Wastewater; Surveillance; False-positive; False-negative; RT-PCR

Online: 19 April 2021 (13:08:13 CEST)

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Wastewater surveillance for pathogens using the reverse transcription-polymerase chain reaction (RT-PCR) is an effective, resource-efficient tool for gathering additional community-level public health information, including the incidence and/or prevalence and trends of coronavirus disease-19 (COVID-19). Surveillance of SARS-CoV-2 in wastewater may provide an early-warning signal of COVID-19 infections in a community. The capacity of the world’s environmental microbiology and virology laboratories for SARS-CoV-2 RNA characterization in wastewater is rapidly increasing. However, there are no standardized protocols nor harmonized quality assurance and quality control (QA/QC) procedures for SARS-CoV-2 wastewater surveillance. This paper is a technical review of factors that can lead to false-positive and -negative errors in the surveillance of SARS-CoV-2, culminating in recommendations and strategies that can be implemented to identify and mitigate these errors. Recommendations include, stringent QA/QC measures, representative sampling approaches, effective virus concentration and efficient RNA extraction, amplification inhibition assessment, inclusion of sample processing controls, and considerations for RT-PCR assay selection and data interpretation. Clear data interpretation guidelines (e.g., determination of positive and negative samples) are critical, particularly during a low incidence of SARS-CoV-2 in wastewater. Corrective and confirmatory actions must be in place for inconclusive and/or potentially significant results (e.g., initial onset or reemergence of COVID-19 in a community). It will also be prudent to perform inter-laboratory comparisons to ensure results are reliable and interpretable for ongoing and retrospective analyses. The strategies that are recommended in this review aim to improve SARS-CoV-2 characterization for wastewater surveillance applications. A silver lining of the COVID-19 pandemic is that the efficacy of wastewater surveillance was demonstrated during this global crisis. In the future, wastewater will play an important role in the surveillance of a range of other communicable diseases.

Preprint ARTICLE | doi:10.20944/preprints201608.0035.v1

Real-Time Performance of Mechatronic PZT Module Using Active Vibration Feedback Control

Francesco Aggogeri, Alberto Borboni, Angelo Merlo, Nicola Pellegrini, Raffaele Ricatto

Subject: Engineering, Industrial And Manufacturing Engineering Keywords: real-time control; mechatronics; PZT actuators; vibration; hardware-in-the-loop

Online: 4 August 2016 (06:20:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0423.v1

Detection of c.375A>G, c.385A>T, c.571C>T, and Sedel2 of FUT2 by Real-time PCR in a Single Tube

Mikiko Soejima, Yoshiro Koda

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Alu-mediated nonhomologous recombination; FUT2; sedel2; c.375A>G; c.385A>T; c.571C>T; Polynesian population

Online: 17 April 2023 (07:43:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202405.0843.v1

Real-World Data on Neutralizing Antibody and Safety of COVID-19 Vaccine against SAR-CoV2 Wild Type and Omicron Variant in Solid Cancer Patients

Busayamas Chewaskulyong, Pattarapong Satjaritanun, Apichat Tantraworasin, Thanika Ketpueak, Thatthan Suksombooncharoen, Chaiyut Charoentum, Nuttaphoom Nuchpong

Subject: Immunology And Microbiology, Biology And Life Sciences Keywords: COVID-19; COVID-19 vaccines; seroconversion rate of neutralizing antibody; Solid cancer patients

Online: 13 May 2024 (12:14:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0980.v1

Impact of Utilizing Visual Stimuli (BlazePod) on Agility, Vertical jump, and Visual Reaction Time Speed in Under-19 Volleyball Players

Youssef Zekailiche, Houda Bougrine, Jihen Khalfoun, Nidhal Jebabli, Rachad Djeddi, Foued Saad Saoud, Abderraouf Ben Abderrahman

Subject: Public Health And Healthcare, Other Keywords: Blazepod technology; Agility; Speed visual reaction time; Vertical Jump; Volleyball Physical skills

Online: 18 March 2024 (09:51:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0621.v1

Occurrence of Picobirnavirus in Domestic and Wild Animals from Three Cities of Brazilian Amazon

Elaine Hellen Nunes Chagas, Julia Rezende Da Silva, Bruno de Cássio Veloso de Barros, José Wandilson Barboza Duarte Júnior, Fabiolla da Silva dos Santos, Edivaldo Costa Sousa Júnior, Delana Andreza Melo Bezerra, Helder Henrique Costa Pinheiro, Ceyla Maria Oeiras de Castro, Yashpal Singh Malik, Joana D’Arc Pereira Mascarenhas

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: enteric virus; picobirnavirus; brazilian animals; page, rt-pcr; rdrp gene; phylogenetic analysis; genogroup I

Online: 9 November 2023 (11:24:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1733.v1

Improvement of Raw and Pasteurized Milk Quality Through the Use of Lactoperoxidase System (LPS) Along Dairy Value Chain Under Real Condition in Ethiopia

Tigist Ashenafi, Haftom Zebib, Ashagrie Zewdu Woldegiorgis

Subject: Biology And Life Sciences, Food Science And Technology Keywords: LPS; Total Bacterial Count; Raw Milk; Pasteurized Milk; E.coli

Online: 29 March 2024 (04:42:35 CET)

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Preprint ARTICLE | doi:10.20944/preprints201611.0048.v2

Uridine Triphosphate Thio-analogues Inhibit Platelet P2Y₁₂ Receptors and Aggregation

Dursun Guenduez, Christian Tanislav, Daniel Sedding, Mariana Parahuleva, Sentot Santoso, Christian Troidl, Christian Hamm, Muhammad Aslam

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: 2S-UTP; 4S-UTP; P2Y12 receptors; ADP; platelet aggregation

Online: 28 December 2016 (10:46:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0522.v1

Analysis of a Relaxation Process Caused by the Load Variation Adapted on the Basis of Second Order Electrical Model

Guzel Nikishina, Evgeny Denisov

Subject: Engineering, Electrical And Electronic Engineering Keywords: lithium battery; electrochemical power sources; diagnostics; battery management system; load variation; in situ method

Online: 31 December 2021 (12:59:15 CET)

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Preprint ARTICLE | doi:10.20944/preprints202208.0209.v1

Real-Time and Historical Viral Variant Tracking

James Labadorf

Subject: Biology And Life Sciences, Virology Keywords: cluster analysis; SARS-CoV-2; Variant

Online: 11 August 2022 (06:01:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201607.0071.v1

An Implementation of Real-Time Phased Array Radar Fundamental Functions on DSP-Focused, High-Performance Embedded Computing Platform

Xining Yu, Yan Zhang, Ankit Patel, Allan Zahrai, Mark Weber

Subject: Engineering, Electrical And Electronic Engineering Keywords: phased array radar; embedded computing; serial RapidIO, MPAR

Online: 23 July 2016 (10:43:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0141.v1

Real Time Road Monitoring System

Muhammad Ayoub Kamal

Subject: Computer Science And Mathematics, Information Systems Keywords: rural areas; urban areas; cloud server; monitoring; road monitoring

Online: 10 March 2022 (07:47:15 CET)

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Preprint REVIEW | doi:10.20944/preprints201912.0072.v1

Scheduling in Real Time System

mehreen sirshar, huma rizwan, muneeba shabbir, hajra nawaz

Subject: Computer Science And Mathematics, Information Systems Keywords: Sporadic tasks; fault tolerance; scheduling; real time system; virtualized clouding; petri net; distributive systems

Online: 5 December 2019 (11:50:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0573.v1

A Surgical Pen-Type Probe Design for Real-Time Optical Diagnosis of Tumor Status using 5-Aminolevulinic acid

Ki-Cheol Yoon, Kwang Gi Kim, Seung Hoon Lee

Subject: Engineering, Automotive Engineering Keywords: fluorescence microscopy; fluorescence emission, malignant tumor, diagnosis, animal experiment

Online: 21 April 2021 (11:47:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202106.0168.v1

Dimensions of knowledge in prototyping: a review and characterisation of prototyping methods and their contributions to design knowledge

Ricardo Real

Subject: Engineering, Automotive Engineering Keywords: Design knowledge, Knowledge dimensions, prototyping, design cognition, design thinking

Online: 7 June 2021 (11:30:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.2092.v1

Pathogenic Biofilm Removal Potential of Wild-Type Lacticaseibacillus Rhamnosus Strains

Gregoria Mitropoulou, Vasiliki Kompoura, Grigorios Nelios, Yiannis Kourkoutas

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Biofilm removal; L. rhamonsus; cell free supernatant; foodborne pathogens; probiotics

Online: 31 October 2023 (12:24:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints201610.0072.v1

Corrosion of Fe-(9~37) wt.%Cr Alloys at 700-800 ^oC in (N₂, H₂O, H₂S)-Mixed Gas

Min Jung Kim, Muhammad Ali Abro, Yuke Shi, Dong Bok Lee

Subject: Chemistry And Materials Science, Metals, Alloys And Metallurgy Keywords: Fe-Cr alloy; oxidation; sulfidation; H2S corrosion

Online: 18 October 2016 (08:04:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202112.0509.v1

Development and Validation of a One-Step Reverse Transcription Real-Time PCR Assay for Simultaneous Detection and Identification of Tomato Mottle Mosaic Virus and Tomato Brown Rugose Fruit Virus

Antonio Tiberini, Ariana Manglli, Anna Taglienti, Ana Vučurović, Jakob Brodaric, Luca Ferretti, Marta Luigi, Andrea Gentili, Nataša Mehle

Subject: Biology And Life Sciences, Plant Sciences Keywords: ToBRFV; ToMMV; Solanum lycopersicum; Capsicum annum; leaves detection; seeds detections; performance criteria

Online: 31 December 2021 (11:12:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1908.v1

Deep Dive Into the DNA Polymerase Through Insilico Analysis: An Information to Get Better PCR Enzyme From the Ancient One

Debanjan Mitra

Subject: Biology And Life Sciences, Other Keywords: Polymerase chain reaction; DNA polymerases; Intra-protein interactions; Protein satbility

Online: 27 June 2023 (12:46:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202006.0063.v1

COVID-19 Real-Time Tracker and Analytical Report

Jiawei Long

Subject: Computer Science And Mathematics, Probability And Statistics Keywords: COVID-19; Real-Time Tracker; Common Symptoms; Data Visualization; Hypothesis Testing; ARIMA Time-Series Forecast; Penalized Logistic Regression

Online: 7 June 2020 (07:44:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0688.v1

Direct Quantitation of SARS-CoV-2 Using Droplet Digital PCR in Suspected Samples With Very Low Viral Load

Michela Deiana, Chiara Piubelli, Antonio Mori, Gian Paolo Chiecchi, Giulia La Marca, Martina Leonardi, Davide Treggiari, Anna Beltrame, Lucia Moro, Zeno Bisoffi, Elena Pomari

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: droplet digital PCR; real time RT-PCR; SARS-CoV-2; false negative; viral load; diagnosis

Online: 26 April 2021 (20:09:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202201.0020.v1

Advanced Strategies for Therapeutic Targeting of Wild-Type and Mutant p53 in Cancer

Shengliang Zhang, Lindsey Carlsen, Liz Hernandez Borrero, Attila A. Seyhan, Xiaobing Tian, Wafik S. El-Deiry

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: P53; mutant p53; targeting therapy; immunotherapy; cancer

Online: 4 January 2022 (20:34:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0816.v1

Snapshot Optimal Real-time Ride Sharing

Afzaal Hassan, Mark Wallace, Irene Moser, Daniel Harabor

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Ridesharing; Ridematching; Shared Mobility

Online: 14 February 2024 (13:50:20 CET)

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Preprint ARTICLE | doi:10.20944/preprints201711.0087.v3

Real-Life/Real-Time Elderly Fall Detection with a Triaxial Accelerometer

Angela Sucerquia, Jose David López, Francisco Vargas-Bonilla

Subject: Public Health And Healthcare, Public Health And Health Services Keywords: triaxial accelerometer; wearable devices; fall detection; mobile health-care; SisFall; Kalman filter

Online: 6 February 2018 (05:37:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0422.v1

Site-Dependency of Anodal tDCS on Reaction Time and Transfer of Learning during a Sequential Visual Isometric Pinch Task

Fahimeh Hashemirad, Maryam Zoghi, Paul B Fitzgerald, Masoumeh Hashemirad, Shapour Jaberzadeh

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: anodal transcranial direct current stimulation; a-tDCS; Reaction time; transfer learning; primary motor cortex; dorsolateral prefrontal cortex; posterior parietal cortex

Online: 8 March 2024 (01:55:26 CET)

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Noninvasive brain stimulation technique can be used for modulation of motor behaviors such as reaction time (RT). The main aim of this study was to investigate whether RT during a sequential visual isometric pinch task (SVIPT) can be affected by anodal transcranial direct current stimulation (a-tDCS) over three different stimulation sites such as dorsolateral prefrontal cortex (DLPFC), primary motor cortex (M1) or posterior parietal cortex (PPC). We also aimed to assess if stimulation of these cortical sites affect the transfer of learning during SVIPT. A total of 48 right-handed healthy participants were randomly assigned to one of the four a-tDCS groups: 1) left M1, 2) left DLPFC, 3) left PPC and 4) sham. A-tDCS was applied concurrent with SVIPT in which the participants were precisely controlled their forces to reach seven different target forces from 10 to 40% of maximum voluntary contraction (MVC) presenting on a computer screen with right dominant hand. Four test blocks were randomly performed at baseline and 15 min after the intervention including sequence and random blocks with either hand. Our results showed significant differences between a-tDCS groups on ratio RT in the sequence blocks of both the right trained (P = .041) and left untrained hands (P = .023) for target forces of 15% and 30 % of MVC, respectively. In sequence blocks, M1 showed significant elongation in ratio RT for the target force of 15% and 30 % MVC compared to PPC and Sham groups in the right hand. In sequence blocks with left hand, this negative effect were found between M1-DLPFC and M1-Sham groups. In random blocks, no significant differences were found among four groups at each target forces for the left hand while there were significant differences for 15% (P =. 04) and 40% MVC (P =. 035) in the right hand. Our findings suggest that three different stimulation sites (DLPFC, M1 or PPC) were differentially affected by a single session of a-tDCS at some target forces during SVIPT. The effects of a-tDCS on ratio RT were transferred in the left untrained hand for sequence blocks but not random blocks. Further research is needed to understand fundamental aspects of these main areas of the brain on temporal processing in a precision control task such as SVIPT.

Preprint CASE REPORT | doi:10.20944/preprints202403.0899.v1

A Study on the Factors for the Occurrence of Vacant Homes in Medium Cities and Characteristics of Each Types – Focusing on Asan City, Chungcheongnam-Do

Jeonghyeon Choi, Seung-Seok Han, Myung-je Woo

Subject: Social Sciences, Urban Studies And Planning Keywords: vacant home; poisson; negative binomial regression; spatial regression

Online: 15 March 2024 (07:48:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202104.0557.v1

Design of a Surgical Pen-Type Probe for Real-Time Diagnosis of Vascular Flow Using Indocyanine Green (ICG) Fluorescence Dye

Ki-Cheol Yoon, Kwang Gi Kim, Seung Hoon Lee

Subject: Engineering, Automotive Engineering Keywords: fluorescence microscopy; fluorescence emission; malignant tumor; diagnosis; animal experiment

Online: 21 April 2021 (08:30:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201701.0075.v1

How Do Power Type and Partnership Quality Affect Supply Chain Management Performance?

Kwang O. Park, Hwalsik Chang, Dae Hyun Jung

Subject: Business, Economics And Management, Business And Management Keywords: SCM; SCM performance; mediated power; non- mediated power; partnership quality

Online: 16 January 2017 (10:21:48 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1527.v1

The Functional Roles of MDSCs in Severe COVID-19 Pathogenesis

Kuan Rong Chan, Jia Soon Len, Clara WT Koh

Subject: Biology And Life Sciences, Virology Keywords: SARS-CoV-2; COVID-19; myeloid-derived suppressor cells; infection; innate immunity; T cells

Online: 23 November 2023 (11:18:17 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1577.v1

Production of fine chemicals using microporous and mesoporous heterogeneous catalysts

Joseph Lantos, Narendra Kumar, Basudeb Saha

Subject: Chemistry And Materials Science, Chemical Engineering Keywords: Fine chemicals, speciality chemicals, heterogeneous catalysis, microporous and mesoporous catalysts, metal organic frameworks (MOFs), zeolites, ion exchange, agrochemicals, fragrances, pharmaceuticals.

Online: 28 February 2024 (00:07:30 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.0197.v1

Quantitative analysis of desertification driving mechanisms in the Shiyang River Basin: Examining interactive effects of key factors through the Geographic Detector Model

Maurice Ngabire, Tao Wang, Jie Liao, Ghada Sahbeni

Subject: Environmental And Earth Sciences, Remote Sensing Keywords: Desertification; Geographical Detector Model; Google Earth Engine; Driving factors; The Shiyang River Basin

Online: 11 April 2023 (05:15:47 CEST)

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Desertification is a global environmental and socio-economical issue threatening humanity's survival and development. The Shiyang River Basin ecosystem is vulnerable and prone to desertification. In addition, establishing the quantitative analysis of desertification driving factors and understanding their relative contribution, separately or combined, is still an unresolved problem. The present study applied geographic information system (GIS) techniques and a geographic detector model to quantify desertification spatial extent and driving mechanisms. This research utilized Fractional Vegetation Cover (FVC) to elucidate desertification spatial heterogeneity. The 30 years Coefficient of Variation (CV) of the Normalized Difference Vegetation Index (NDVI) was a dependent variable and indicator of ecosystem terrestrial conditions; Elevation, near-surface air temperature, precipitation, wind velocity, land cover change, soil salinity, road buffers, waterway buffers, and soil types were independent variables. The results showed that 89.41% of the total area is under desertification risk, where 20.99% is extremely desertified, 34.45% is severely desertified, 12.05% is moderately, and 21.92% is slightly desertified. The results from the Geodetector model showed that Power Determinant (PD) values ranged between 0.004 and 0.270. Elevation and soil types had the highest contributing factors with PD values of 0.270 and 0.227, whereas precipitation, soil salinity, the buffer of the waterway, and wind velocity played a moderate role with PD values of 0.146, 0.117, 0.107, and 0.071. Near-surface air temperature, road buffer, and land cover dynamics exhibited lower impact with PD values of 0.028, 0.013, and 0.004. In most cases, investigating the interaction between driving factors resulted in a mutual or non-linear enhancement. There was an apparent linear and mutual enhancement between elevation and soil salinity, precipitation, and soil types with values of 0.3513, 0.3232, and 0.3204, respectively. In addition, there was a mutual enhancement between soil salinity and soil types with a value of 0.2962. On the other hand, a non-linear enhancement was observed between Elevation and near-surface air temperature (0.3116), Elevation and Land cover dynamics (0.2759), soil types and near-surface air temperature (0.2687), land cover dynamics and soil types (0.234), precipitation and near-surface air temperature (0.2248), precipitation and wind velocity (0.2248), and between land cover dynamics and precipitation (0.223). This research revealed irrefutable evidence that environmental factors might be the primary drivers of ecosystem disturbance, provided the basis for the environmental footprint of desertification mechanism, and might be a cornerstone for future policy on ecological restoration sustainability in the Shiyang River Basin.

Preprint ARTICLE | doi:10.20944/preprints202010.0600.v1

Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report

Barbara Sterczała, Kinga Grzech-Leśniak, Olga Michel, Witold Trzeciakowski, Kamil Jurczyszyn

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: cell proliferation; human gingival fibroblasrs; photobiomodulation PBM; soft tissue regeneration

Online: 29 October 2020 (09:20:47 CET)

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Preprint ARTICLE | doi:10.20944/preprints201901.0053.v1

Doppler Shift Time Expansion Resolution and Spectral Performance in Wideband Real-Time RF Channel Emulators

Massimiliano Rossi

Subject: Engineering, Electrical And Electronic Engineering Keywords: channel emulator; doppler; propagation channel

Online: 7 January 2019 (10:38:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints201610.0022.v1

Transcriptome Profiling Analysis of Wolf Spider Pardosa pseudoannulata (Araneae: Lycosidae) after Cadmium Exposure

Chang-chun Li, Yong Wang, Guo-yuan Li, Yueli Yun, Yu-jun Dai, Jian Chen, Yu Peng

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: P. pseudoannulata; Cadmium; Transcriptome; RT-qPCR

Online: 8 October 2016 (11:07:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0109.v1

A Compact Real-time Quantitative Phase Imaging System

Tao Peng, Zeyu Ke, Shuhe Zhang, Meng Shao, Han Yang, Xiao Liu, Huijuan Zou, Zhensheng Zhong, Mincheng Zhong, Hui Shi, Shu Fang, Rongsheng Lu, Jinhua Zhou

Subject: Physical Sciences, Optics And Photonics Keywords: self-design setup; real-time imaging; GPU acceleration; quantitative phase imaging; differential phase contrast microscopy

Online: 12 April 2022 (10:19:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202201.0159.v1

Real Time Fault Location Using the Retardation Method

Moneer Nabwani, Michael Suleymanov, Yosef Pinhasi, Asher Yahalom

Subject: Engineering, Electrical And Electronic Engineering Keywords: fault location; real time

Online: 12 January 2022 (09:50:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202105.0762.v1

Real-Time Smoke Simulation based on Vortex Method

Fei Wan, Jingpu Zhang, Lizheng Guo, Yunchang Liu

Subject: Computer Science And Mathematics, Mathematics Keywords: smoke simulation; vortex method; Vortex-In-Cell (VIC)

Online: 31 May 2021 (12:11:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0036.v1

Real-Time Quality Index to Control Data Loss in Real-Life Cardiac Monitoring Applications

Gaël Vila, Christelle Godin, Sylvie Charbonnier, Aurélie Campagne

Subject: Engineering, Automotive Engineering Keywords: wearable cardiac sensors; electrocardiography; photoplethysmography; heart rate variability; signal quality; real-life measurements

Online: 1 July 2021 (15:40:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202107.0456.v1

Benefits of a Switch From Intermittently Scanned Continuous Glucose Monitoring (isCGM) to Real-time (rt) CGM in Diabetes Type 1 Suboptimal Controlled Patients in Real-life: A One-year Prospective Study

Yannis Préau, Sébastien Galie, Pauline Schaepelynck, Martine Armand, Denis Raccah

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: Real-time continuous glucose monitoring; Intermittently scanned continuous glucose monitoring; Type 1 diabetes; Glucose variability; Hypoglycemia; Insulin resistance

Online: 20 July 2021 (14:58:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1112.v1

Cellular Immunity of SARS-CoV-2 in the Borriana COVID-19 Cohort: A Nested Case-Control Study

Salvador Domenèch-Montoliu, Joan Puig-Barberà, Maria Rosario Pac-Sa, Alejandro Orrico-Sanchez, Lorna Gómez-Lanas, Diego Sala-Trull, Carmen Domènech-León, Alba Del Rio-González, Manuel Sanchez-Urbano, Paloma Satorres-Martinez, Laura Aparisi-Esteve, Gema Badenes-Marques, Roser Blasco-Gari, Juan Casanova-Suarez, Maria Gil-Fortuño, Noelia Hernández-Pérez, David Jovani-Sales, Laura López-Diago, Cristina Notari-Rodríguez, Oscar Pérez-Olaso, María Angeles Romeu-Garcia, Raquel Ruíz-Puig, Alberto Arnedo-Pena

Subject: Medicine And Pharmacology, Epidemiology And Infectious Diseases Keywords: COVID-19; SARS-CoV-2 infection; cellular immunity; T cells; CD4+; CD8+; humoral immunity; Anti-S antibodies Anti-N antibodies; nested case-control; associated factors

Online: 20 February 2024 (09:01:39 CET)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202401.1055.v1

Use of the Investigator ESSplex SE QS Kit (QIAGEN) at half PCR Reaction volumes for the analysis of forensic samples

Anna Barbaro, Giacomo Falcone, Angelo La Marca, Aldo Barbaro

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: ESSplexQS; STR multiplex; half reaction volumes

Online: 15 January 2024 (10:40:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0319.v1

RDCP: A Real Time Sea Fog Intensity and Visibility Estimation Algorithm

Shin Hyuk Hwang, Sung Hyun Park, Ki Won Kwon, Sekil Park, Taeho Im

Subject: Environmental And Earth Sciences, Oceanography Keywords: sea fog intensity estimation; visibility estimation; sea fog image processing

Online: 6 November 2023 (07:47:52 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0124.v1

Robot Navigation Algorithm Based on Real-Time Ranging and Map Matrix

Linwei Cui, Jianwei Cui, Zizheng Huang, Xiang Li, Yucheng Shang, Liyan Tong

Subject: Engineering, Control And Systems Engineering Keywords: Intelligent archive repository; archive access robot; complex digital filtering; map matrix; navigational mobility

Online: 7 November 2022 (12:23:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202109.0285.v1

Autonomous and Real-time Rock Image Classification using Convolutional Neural Networks

Alexis David Pascual, Kenneth McIsaac, Gordon Osinski

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: remote sensing; deep learning; image classification

Online: 16 September 2021 (13:38:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202403.0717.v1

Potential Inhibitors of Lumpy Skin Disease’s Viral Protein (DNA Polymerase): A Combination of Bioinformatics Approaches

Sabbir Zia, Md-Mehedi Sumon, Md-Ashikur Ashik, Abul Basar, Sangjin Lim, Yeonsu Oh, Yungchul Park, Md Mafizur Rahman

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Lumpy skin disease; DNA polymerase; potential inhibitors; phytocompounds; antiviral drug compounds; molecular docking

Online: 13 March 2024 (07:58:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202110.0097.v1

Process Analysis of PMMA Dental Resins Scraps Depolimerization: Optimization of Reaction Time and Temperature

Paulo Bisi dos Santos Jr., Haroldo Jorge da Silva Ribeiro, Armando Costa Ferreira, Caio Campos Ferreira, Lucas Pinto Bernar, Douglas Alberto Rocha de Castro, Fernanda Paula da Costa Assunção, Marcelo Costa Santos, Sergio Duvoisin Jr., Luiz Eduardo Pizaro Borges, Nelio Teixeira Machado

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: PMMA; Pyrolysis; Plastics Wastes; Depolymerization; Distillation; Recovery of Methylmethac-rylate

Online: 6 October 2021 (09:19:43 CEST)

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In this work, the cross-linked PMMA-based dental resins scraps were submitted to pyrolysis to recover MMA (Methylmethacrylate). The thermal degradation of cross-linked PMMA-based dental resins scraps was analyzed by TG/DTG to guide the operating conditions in pilot scale. The pyrolysis experiments carried out in a reactor of 143L, at 345, 405, and 420°C, 1.0 atmosphere. The reaction liquid products obtained at 345°C, physicochemical characterized for density, kinematic viscosity, and refractive index. The chemical composition of liquid products obtained at 345°C, 30, 40, 50, 60, 70, 80, and 110 minutes, at 405°C, 50, 70, and 130 minutes, and at 420°C, 40, 50, 80, 100, 110, and 130 minutes determined by GC-MS. The experiments show that liquid phase yields were 55.50%, 48.73%, and 48.20% (wt.), at 345, 405, and 420°C, respectively, showing a smooth sigmoid behavior, decreasing with increasing temperature, while that of gas phase were 31.69%, 36.60%, and 40.13% (wt.), respectively, increasing with temperature. The liquid products density, kinematic viscosity, and refractive index obtained at 30, 40, 50, 60, 70, 80, and 110 minutes, varied between 0.9227 and 0.9380 g/mL, 0.566 and 0.588 mm2/s, and 1.401 and 1.414, respectively, showing percentage deviations between 0.74 and 2.36%, 7.40 and 10.86%, and 0.00 and 0.92%, respectively, compared to standard values for density, kinematic viscosity, and refractive index of pure MMA at 20 °C. The GC-MS identified in the reaction liquid products at 345, 405, and 420°C, 1.0 atm, esters of carboxylic acids, alcohols, ketones, and aromatics, showing concentrations of MMA between 83.454 and 98.975% (area.). For all the depolymerization experiments, the concentrations of MMA in the liquid phase, between 30 and 80 minutes, reach purities above 98% (area.), decreasing drastically with increasing reaction time after 100 minutes, thus making it possible to depolymerize the cross-linked PMMA-based dental resins scraps by pyrolysis to recover MMA. The optimum operating conditions to achieve high MMA concentrations, as well as elevated yields of liquid reaction products were 345 °C and 80 minutes.

Preprint ARTICLE | doi:10.20944/preprints202311.0110.v1

Efficient Human Violence Recognition for Surveillance in Real-Time

Herwin Alayn Huillcen Baca, Flor de Luz Palomino Valdivia, Juan Carlos Gutierrez Caceres

Subject: Computer Science And Mathematics, Artificial Intelligence And Machine Learning Keywords: Human violence recognition; video surveillance; real-time; spatial attention; spatial motion extractor; short temporal extractor; global temporal extractor; VioPeru

Online: 2 November 2023 (04:06:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.1425.v1

Towards Real-time 3D Terrain Reconstruction from Aerial Imagery

Qiaosong Wang

Subject: Computer Science And Mathematics, Computer Vision And Graphics Keywords: 3D Reconstruction; Unmanned Aerial Vehicles; Real-time Systems

Online: 21 August 2023 (05:31:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202303.0031.v1

A Mixed Clustering Approach for Real Time Anomaly Detection

Fokrul Alom Mazarbhuiya, Mohamed Shenify

Subject: Computer Science And Mathematics, Data Structures, Algorithms And Complexity Keywords: data instances; real time systems; k-means algorithm; agglomerative hierarchical algorithm; similarity measure; merge function ;

Online: 2 March 2023 (04:15:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0508.v1

Real-Time Interpolated Rendering of Terrain Point Cloud Data

Jaka Kordež, Matija Marolt, Ciril Bohak

Subject: Computer Science And Mathematics, Software Keywords: point clouds; interpolation methods; terrain rendering

Online: 28 November 2022 (09:56:51 CET)

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Preprint ARTICLE | doi:10.3390/sci2010006

Making Japenese Ukiyo-e Art 3D in Real-Time

Innes Brown, Ognjen Arandjelović

Subject: Computer Science And Mathematics, Signal Processing Keywords: rendering; graphics; heritage; Japanese; Asian

Online: 28 February 2020 (00:00:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints201909.0100.v1

Face Detection with a portable architecture in Real Time

Francesco De Feo, Pasquale De Luca

Subject: Computer Science And Mathematics, Robotics Keywords: Face detection; Drone; Real Time

Online: 9 September 2019 (12:04:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201808.0407.v1

Real Time Elbow Angle Estimation Using Single RGB Camera

Muhammad Yahya, Jawad Ali Shah, Arif Warsi, Kushsairy Kadir, Sheroz Khan, Mohamad Izani

Subject: Engineering, Control And Systems Engineering Keywords: angle estimation; microsoft kinect; single camera; markerless mocap system

Online: 23 August 2018 (05:55:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201804.0056.v1

Real-Time Demand Side Management Algorithm Using Stochastic Optimization

Moses Amoasi Acquah, Daisuke Kodaira, Sekyung Han

Subject: Engineering, Electrical And Electronic Engineering Keywords: demand-side management; peak demand control; dynamic-interval density forecast; stochastic optimization; dimension reduction; battery energy-storage system (BESS)

Online: 4 April 2018 (08:37:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202104.0460.v1

Reaction Time and Visual Memory in Connection to Alcohol Use In Schizophrenia and Schizoaffective Disorder

Atiqul Haq Mazumder, Jennifer Barnett, Nina Lindberg, Minna Torniainen-Holm, Markku Lähteenvuo, Kaisla Lahdensuo, Martta Kerkelä, Jarmo Hietala, Erkki Isometsä, Olli Kampman, Tuula Kieseppä, Tuomas Jukuri, Katja Häkkinen, Erik Cederlöf, Willehard Haaki, Risto Kajanne, Asko Wegelius, Teemu Männynsalo, Jussi Niemi-Pynttäri, Kimmo Suokas, Jouko Lönnqvist, Solja Niemelä, Jari Tiihonen, Tiina Paunio, Annamari Tuulio-Henriksson, Aarno Palotie, Jaana Suvisaari, Juha Veijola

Subject: Medicine And Pharmacology, Immunology And Allergy Keywords: Cognition; Visual memory; Reaction time; Alcohol; schizophrenia and schizoaffective disorder.

Online: 19 April 2021 (11:26:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202402.1600.v1

The Relationship between Supply chain Resilience and Digital Supply Chain on Sustainability, Supply Chain Dynamism as a Moderator

Ahmad Ali Atieh Ali, Abdel-Aziz Ahmad Sharabati, Mahmoud Allahham, Ahmad Yacoub Nasereddin

Subject: Business, Economics And Management, Business And Management Keywords: Supply chain Resilience; Digital Supply chain; Supply Chain Sustainability; Supply Chain Dynamism

Online: 28 February 2024 (10:53:55 CET)

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This research aims to explore the complex interplay between supply chain resilience (SCR), digital supply chain (DSC), and sustainability, focusing on the moderating influence of supply chain dynamism. The goal is to understand how these elements interact within the framework of contemporary supply chain management and how they collectively contribute to enhancing sustainability outcomes. The sample size is 300 CEOs and managers. The study approach integrates quantitative research methods. Structural equation modeling (SEM) is utilized to quantitatively analyze the direct and indirect effects of SCR and DSC on sustainability. The numerous surveys we conduct among supply chain ecosystem stakeholders provide a rich picture of practical implications and contextual nuances. In sum, our early findings generally support a positive relationship between SCR and sustainability in and of itself decrying the need for more resilient supply networks for sustainability. We further find the beneficial impact of digital technologies in promoting sustainability via enhancing environmental control and controlling for efficiency in supply chains. We also offer evidence to show that supply chain dynamism compounds the positive logic between SCR and DSC and sustainability. As a final word, it must be noted that our work speaks to the burgeoning Supply Chain Dynamism as moderator literature by examining the direct and contingent effects of SCR and DSC on not only performance but sustainability. By shedding light on the moderating role of dynamism, the study provides fresh insights into the multifaceted nature of supply chain management and sustainability practices. The study's findings enhance theoretical understanding by elucidating the synergistic effects of SCR, DSC, and sustainability in dynamic supply chain settings. It augments existing theoretical frameworks by integrating concepts of resilience, digitalization, and sustainability into a comprehensive model. Practical and economical, the research offers actionable guidance for organizations aiming to improve sustainability performance through resilient and digitally advanced supply chains. By acknowledging the role of supply chain dynamism, managers can tailor strategies to manage disruptions effectively and leverage digital innovations. Economically, adopting sustainable practices can result in cost savings and competitive advantages. The research emphasizes the importance of aligning supply chain strategies with sustainability goals to drive long-term value and societal impact.

Preprint ARTICLE | doi:10.20944/preprints202402.1470.v1

Whey Mediated Synthesis of Silver Nanoparticles, and Optimization of Reduction Reaction Conditions

Christina Megetho Gkaliouri, Nickolas Rigopoulos, Nikolaos Chalmpes, Viktoria Sakavitsi, Dimitrios Gournis

Subject: Chemistry And Materials Science, Nanotechnology Keywords: silver nanoparticles; green synthesis; reduction; response surface methodology; cheese Whey

Online: 26 February 2024 (15:27:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1446.v1

Hardware in the Loop Simulation of Gas Turbines: How Real Time Control System Design Tools Can Be Exploited Also to Generate Fault Cases to Train and Tune Data Based Diagnostic Systems.

Attilio Brighenti, Chiara Brighenti

Subject: Engineering, Mechanical Engineering Keywords: HIL model(s); Dynamic simulation(s); Data-based modelling; Predictive diagnostics; Fault detection and isolation (FDI)

Online: 25 March 2024 (08:30:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints201703.0165.v1

Effectiveness Regulation of Construction Land: Does Plan Matter?

Guan Li, Cifang Wu, Yuefei Zhuo, Xinhua Tong, Yanfei Wei, Rucheng Lu

Subject: Social Sciences, Geography, Planning And Development Keywords: Regulation Effectiveness; Land Use Plan; Construction Land; China

Online: 20 March 2017 (18:42:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202308.0643.v1

FPGA-based Feature Extraction and Tracking Accelerator for Real-Time Visual SLAM

Jie Zhang, Shuai Xiong, Cheng Liu, Yongchao Geng, Wei Xiong, Song Cheng, Fang Hu

Subject: Engineering, Electrical And Electronic Engineering Keywords: VIO; V-SLAM; FPGA; histogram equalization; FAST; Pyramid processing

Online: 9 August 2023 (02:36:25 CEST)

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