PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods
Version 1
: Received: 3 May 2023 / Approved: 4 May 2023 / Online: 4 May 2023 (08:18:05 CEST)
How to cite:
Marin, E.; Messina, F.; Romero, M.; Arechavala, A.; Depardo, R.; Santiso, G. Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods. Preprints2023, 2023050224. https://doi.org/10.20944/preprints202305.0224.v1
Marin, E.; Messina, F.; Romero, M.; Arechavala, A.; Depardo, R.; Santiso, G. Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods. Preprints 2023, 2023050224. https://doi.org/10.20944/preprints202305.0224.v1
Marin, E.; Messina, F.; Romero, M.; Arechavala, A.; Depardo, R.; Santiso, G. Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods. Preprints2023, 2023050224. https://doi.org/10.20944/preprints202305.0224.v1
APA Style
Marin, E., Messina, F., Romero, M., Arechavala, A., Depardo, R., & Santiso, G. (2023). Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods. Preprints. https://doi.org/10.20944/preprints202305.0224.v1
Chicago/Turabian Style
Marin, E., Roxana Depardo and Gabriela Santiso. 2023 "Evaluation of an Enzyme Immunoassay Technique on Detecting Urinary Histoplasma capsulatum Antigen in the Diagnosis of Disseminated Histoplasmosis in Argentina and Comparison With Blood Polymerase Chain Reaction and Conventional Methods" Preprints. https://doi.org/10.20944/preprints202305.0224.v1
Abstract
Histoplasmosis is a systemic mycosis of universal distribution, highly endemic in the Americas. It is caused by a dimorphic fungus Histoplasma capsulatum var. capsulatum. It affects both immunocompetent and immunocompromised individuals where progressive and disseminated forms are observed. A very important risk factor is HIV infection/AIDS, with a mortality rate of 20-40% in Latin America. The diagnosis of this mycosis is made by conventional and molecular methods or by antigen and antibody detection.
This study evaluated the sensitivity and specificity of a commercial kit for the detection of Histoplasma antigen by an EIA (HC-Ag) technique in 50 patients with histoplasmosis associated with AIDS. In addition, its performance was compared with that of other diagnostic techniques routinely used in our laboratory. HC-Ag had a sensitivity (S) of 94%, specificity (E) 95.5%, positive predictive value (PPV) 94% and negative predictive value (NPV) 95.5%. The delay time of the results was 4 days, similar to that of antibody detection and n-PCR and much less than that of blood cultures. The combination of methods improved S and NPV: 100%; with similar values in E and PPV.
The HC-Ag method demonstrated its usefulness in the diagnosis of progressive disseminated histoplasmosis and the combination of methods is a good option to increase sensitivity and decrease the time to reach the diagnosis of certainty. This allows improving the strategy in the management of the disease and decreasing its case-fatality rate.
Keywords
histoplasmosis; Histoplasma antigen; Histoplasma diagnosis; Blood culture; Latin America.
Subject
Medicine and Pharmacology, Epidemiology and Infectious Diseases
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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