PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report
Version 1
: Received: 28 October 2020 / Approved: 29 October 2020 / Online: 29 October 2020 (09:20:47 CET)
How to cite:
Sterczała, B.; Grzech-Leśniak, K.; Michel, O.; Trzeciakowski, W.; Jurczyszyn, K. Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report. Preprints.org2020, 2020100600. https://doi.org/10.20944/preprints202010.0600.v1.
Sterczała, B.; Grzech-Leśniak, K.; Michel, O.; Trzeciakowski, W.; Jurczyszyn, K. Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report. Preprints.org 2020, 2020100600. https://doi.org/10.20944/preprints202010.0600.v1.
Cite as:
Sterczała, B.; Grzech-Leśniak, K.; Michel, O.; Trzeciakowski, W.; Jurczyszyn, K. Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report. Preprints.org2020, 2020100600. https://doi.org/10.20944/preprints202010.0600.v1.
Sterczała, B.; Grzech-Leśniak, K.; Michel, O.; Trzeciakowski, W.; Jurczyszyn, K. Assessment of Human Gingival Fibroblast Proliferation After Laser Stimulation In Vitro Using Different Laser Types and Wavelengths (1064, 980, 635, 450 and 405nm) – Preliminary Report. Preprints.org 2020, 2020100600. https://doi.org/10.20944/preprints202010.0600.v1.
Abstract
Purpose: to assess the effect of photobiomodulation (PBM) on human gingival fibroblast proliferation. Methods: The study was conducted using the primary cell cultures of human fibroblasts collected from systemically healthy donors. Three different laser types: Nd:YAG (1064nm), infrared diode laser (980nm) and prototype led laser emitting 405, 450 and 635nm were used to irradiate fibroblasts. Thanks to the patented structure of that laser, it was possible to irradiate fibroblasts with a beam combining two or three wavelengths. The energy density was 3 J/cm², 25 J/cm², 64 J/cm². The viability and proliferation of cells were determined using the MTT test conducted 24, 48 and 72 hours after laser irradiation. Results: The highest percentage of mitochondrial activity (MA=122.1%) was observed in the group irradiated with the 635nm laser, with an energy density of 64 J/cm² after 48 hours. The lowest percentage of MA (94.0%) was observed in the group simultaneously irradiated with three wavelengths (405 + 450 + 635 nm). The use of the 405nm laser at 25 J/cm² gave similar results to the 635 nm laser. Conclusions: The application of the 635nm and 405nm irradiation caused a statistically significant increase in the proliferation of gingival fibroblasts.
Keywords
cell proliferation; human gingival fibroblasrs; photobiomodulation PBM; soft tissue regeneration
Subject
Medicine and Pharmacology, Immunology and Allergy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
