Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

Ct-Value from qRT-PCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success

Dominik Hadzega
* ,
Klaudia Babisova
,
Michaela Hyblova
ORCID logo ,
Nikola Janostiakova
,
Peter Sabaka
,
Pavol Janega
,
Gabriel Minárik
Version 1 : Received: 27 June 2023 / Approved: 28 June 2023 / Online: 29 June 2023 (02:12:07 CEST)

A peer-reviewed article of this Preprint also exists.

Hadzega, D.; Babisová, K.; Hyblová, M.; Janostiaková, N.; Sabaka, P.; Janega, P.; Minarik, G. Ct value from RT-qPCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success. Appl. Sci. 2023, 13, 10431. Hadzega, D.; Babisová, K.; Hyblová, M.; Janostiaková, N.; Sabaka, P.; Janega, P.; Minarik, G. Ct value from RT-qPCR Can Predict SARS-CoV-2 Virus Assembly and Lineage Assignment Success. Appl. Sci. 2023, 13, 10431.

Abstract

During the recent pandemics of COVID-19, sequencing technics became powerful tool for gaining information about the SARS-CoV-2 virus and using this knowledge in our advantage. Thanks to this advantage, scientists over the world were able to search for emerging variations, watching the virus evolving in real time. Assembly of the virus genomes is crucial part of getting this kind of useful information. In our study, we sequenced 79 samples from nasopharyngeal swabs of COVID-19 patients. Positivity on disease was evaluated by qRT-PCR. By studying assembly success rate, we noticed, that Ct-value of qRT-PCR can predict success of genome assembly and SARS-CoV-2 lineage assignment. In this work we described the relationship between Ct-value and further steps of genome construction and its assignment to specific viral strain.

Keywords

SARS-CoV-2; RNA genome assembly; RT-qPCR; SPAdes

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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