Liu, Y.; Zhang, C.; Harijati, N.; Diao, Y.; Liu, E.; Hu, Z. Selection and Evaluation of Reference Genes for RT-qPCR Analysis in Amorphophallus Konjac Based on Transcriptome Data. Genes2023, 14, 1513.
Liu, Y.; Zhang, C.; Harijati, N.; Diao, Y.; Liu, E.; Hu, Z. Selection and Evaluation of Reference Genes for RT-qPCR Analysis in Amorphophallus Konjac Based on Transcriptome Data. Genes 2023, 14, 1513.
Liu, Y.; Zhang, C.; Harijati, N.; Diao, Y.; Liu, E.; Hu, Z. Selection and Evaluation of Reference Genes for RT-qPCR Analysis in Amorphophallus Konjac Based on Transcriptome Data. Genes2023, 14, 1513.
Liu, Y.; Zhang, C.; Harijati, N.; Diao, Y.; Liu, E.; Hu, Z. Selection and Evaluation of Reference Genes for RT-qPCR Analysis in Amorphophallus Konjac Based on Transcriptome Data. Genes 2023, 14, 1513.
Abstract
Real-time fluorescent quantitative PCR (RT-qPCR) is the most classic and widely used technology for evaluating the expression level of target gene. In order to select the proper internal reference genes for RT-qPCR analysis in Amorphophallus konjac (Araceae), eight candidate internal reference genes, including 25S ribosomal RNA gene (25S rRNA), 18S ribosomal RNA gene (18S rRNA), actin gene (ACT), glyceraldehyde-3-phosphate dehydrogenase gene (GAPDH), Ubiquitin gene (UBQ), β-tubulin gene (β-TUB), eukaryotic elongation factor 1-αgene(eEF-1α), and eukaryotic translation initiation factor 4α-1 gene (eIF-4α) were selected and tested the corresponding expression level in different tissues at different growing stages. The results showed that 25S rRNA, 18S rRNA, and ACT at the reproductive periods, eEF-1α and eIF-4α at the nutritional periods, and eEF-1α, UBQ, and ACT at different leaf developmental periods had a stable level of gene expression, respectively. These results might be useful for the study of gene function in A. konjac.
Biology and Life Sciences, Biology and Biotechnology
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