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Preprint ARTICLE | doi:10.20944/preprints202410.1936.v1

Effects of Cover Crops on Nematode Communities in Spinach Production

Elyse Aubry, Jerry Akanwari, Ping Liang, Walid Ellouze, Jonathan Gaiero, Tahera Sultana

Subject: Plant Sciences, Biology And Life Sciences Keywords: nematode; cover crop; Cowpea; Pearl Millet; nematode feeding group; morphology; DNA metabarcoding

Online: 24 October 2024 (17:09:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1903.v1

Comprehensive Genome-Wide Identification and Expression Profiling of bHLH Transcription Factors in Areca catechu under Abiotic Stress

Akhtar Ali, Noor Muhammad Khan, Jiang Yi Qi, Guangzhen Zhou, Yinglang Wan

Subject: Plant Sciences, Biology And Life Sciences Keywords:

Areca catechu; bHLH gene family; Genome-wide analysis; Expression pattern; Abiotic stresses

Online: 24 October 2024 (11:21:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.1712.v1

Advancing Plant Synthetic Biology Using Multiscale Mathematical Modeling

Abel Lucido, Oriol Basallo, Alberto Marin-Sanguino, Abderrahmane Eleiwa, Emilce Soledad Martinez, Ester Vilaprinyo, Albert Sorribas, Rui Alves

Subject: Biology And Life Sciences, Plant Sciences Keywords: multiscale models; synthetic biology; systems biology; mathematical models; plant science

Online: 22 October 2024 (11:17:02 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1680.v1

Spatial Patterns and Influencing Factors of the Leaf Trait Network of Populus euphratica Along the Main Stream of the Tarim River

Chengzhi Peng, Shiyu Yao, Wenjuan Huang, Jie Wang, Shuangfei Song, Pei Zhang, Peipei Jiao

Subject: Biology And Life Sciences, Plant Sciences Keywords: Populus euphratica; leaf trait network; soil; climate; adaptation strategies

Online: 22 October 2024 (10:20:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1689.v1

A Machine Learning Tool to Non‐Invasively Detect Drought Stress in Plants

Prisha Bhat

Subject: Biology And Life Sciences, Plant Sciences Keywords: drought stress; rice; machine learning tool

Online: 22 October 2024 (08:24:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1625.v1

Peptide AEDL and Glutathione Stimulates Root Development Nicotiana tabacum

Neonila Vasilievna Kononenko, Larisa Ivanovna Fedoreyeva

Subject: Biology And Life Sciences, Plant Sciences Keywords: GSH; peptide AEDL; ROS; stem cells

Online: 21 October 2024 (17:09:51 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202403.0014.v2

Shining a New Light on the Classical Concepts of Carbon-Isotope Dendrochronology

Thomas Wieloch

Subject: Biology And Life Sciences, Plant Sciences Keywords: dendrochronology; tree rings; carbon stable isotopes; whole-molecule isotope analysis; intramolecular isotope analysis; water-use efficiency; paleoclimate reconstruction; plant carbon fluxes

Online: 21 October 2024 (10:38:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1333.v1

Ethnobotanical Knowledge on Medicinal Plants Used For Cutaneous Leishmaniasis in Northeastern Algeria: Application of Multivariate Approach

Amel Bouzabata, Leila Bouzabata, Eman Ramadan Elsharkawy, Mohamed L. Ashour, Aya Guebli, Aya Grabsia, Zihad Bouslama, Geoffrey A. Cordell

Subject: Biology And Life Sciences, Plant Sciences Keywords: leishmaniasis; FactoMineR; contribution

Online: 17 October 2024 (04:21:25 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.1314.v1

Polyamine Seed Priming: the Way for Enhancing Stress Tolerance in Plants

Łukasz Wojtyla, Karolina Wleklik, Sławomir Borek, Małgorzata Garnczarska

Subject: Biology And Life Sciences, Plant Sciences Keywords: abiotic stress; chilling stress; priming memory; rice (Oryza sativa L.); salt stress; seed biology; seed germination

Online: 16 October 2024 (16:06:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1275.v1

Impacts of Climate Change-Induced Temperature Rise on Phenology, Physiology, and Yield in Three Red Grape Cultivars: Malbec, Bonarda, and Syrah

Deolindo L. E. Dominguez, Miguel A. Cirrincione, Leonor Deis, Liliana E. Martínez

Subject: Biology And Life Sciences, Plant Sciences Keywords: climate change; plant physiology; heat waves; Malbec; Bonarda; Syrah

Online: 16 October 2024 (11:43:44 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.1240.v1

The Untapped Potential of Hairy Root Cultures and Their Multiple Applications

Iman Mirmazloum, Aleksandar K. Slavov, Andrey S. Marchev

Subject: Biology And Life Sciences, Plant Sciences Keywords: Specialized metabolites; hairy roots; Rhizobium rhizogenes; elicitation; metabolic engineering, CRISPR/CAS9; bioprocessing; bioreactors; patents; industrial scale

Online: 16 October 2024 (09:40:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1252.v1

Arbuscular Mycorrhizal Fungi as a Salt Bioaccumulation Mechanism in a Neotropical Halophytic Fern to Establish in Saline Soils

Mónica A. Lugo, María A. Negritto, Esteban M. Crespo, Hebe J. Iriarte, Samuel Núñez Samuel Núñez, Luisa F Espinosa, Marcela C. Pagano

Subject: Biology And Life Sciences, Plant Sciences Keywords: thermal ponds; halophytic fern; arbuscular mycorrhizas; Glomus; Glomeromycota; coastal environments; mangroves; Neotropics; plant invasions

Online: 16 October 2024 (09:38:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1242.v1

Depletion of Gibberellin Signaling Accelerates De Novo Root Regeneration in Arabidopsis thaliana, Which Can Rescue Declined Adventitious Root Formation from Erecta Mutant Leaf Explants

Tingting Jing, Qian Xing, Yunfeng Shi, Xuemei Liu, Ralf Müller-Xing

Subject: Biology And Life Sciences, Plant Sciences Keywords: Adventitious roots (ARs); de novo root regeneration (DNRR); Paclobutrazol (PBZ/PAC); Blocking of gibberellin (GA) biosynthesis; LBD16; LRP1

Online: 16 October 2024 (08:30:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1149.v1

HDC1 Promotes Primary Root Elongation by Regulating Auxin and K+ Homeostasis in Response to Low K+ Stress

Xiaofang Kuang, Hao Chen, Jing Xiang, Juan Zeng, Qing Liu, Yi Su, Chao Huang, Ruozhong Wang, Wanhuang Lin, Zhigang Huang

Subject: Biology And Life Sciences, Plant Sciences Keywords: auxin transport; HDC1; low-K+ stress; root growth

Online: 15 October 2024 (12:18:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1144.v1

Suspended Soils Have Common Features Regardless of the Species of Epiphyte or the Place in Which They Were Formed

Alen K. Eskov, Tatiana G. Elumeeva, Violetta A. Viktorova, Nikolay G. Prilepsky, Timur Nizamutdinov, Sergey. A. Kouzov, Evgeny V. Abakumov

Subject: Biology And Life Sciences, Plant Sciences Keywords: Suspended soils; epiphytic plants; tropical forest; Southeast Asia; Hydrological parameters; elemental composition; Stable isotope

Online: 15 October 2024 (12:14:38 CEST)

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The Suspended soils (which are also called canopy soil), associated with epiphytic plants in tropical forest canopies, have a very special type of biological cycle, morphological organization and ecological functional role. To date, only some features of these soils have been described for certain sectors of the tropics, in particular Southeast Asia. It was studies out whether the chemical composition and hydrological parameters depend significantly on the genesis of suspended soil, or they represent a single soil phenomenon with a certain supposed ecosystem function. Suspended soils are characterized by average values of hygroscopic moisture comparable with mineral soils. At the same time the maximum water holding capacity and field moisture capacity reaches hundreds and even thousands of percent which cannot but lead to mesophilization of the microclimate of the near-stem environments. In any case suspended soils are characterized by 4-10 higher values of hydrologic properties than mineral terrestrial soils of the same landscapes. The carbon content in suspended soil materials varied widely and was comparable with that of tropical forest litter and boreal peatlands . At the same time, tropical suspended soils are characterized by very low nitrogen content, which is reflected in very high carbon-to-nitrogen ratios. Such a values are typical of peats and forest litter and indicates a very low degree of humification of organic matter. Soil pH values tends to the acidic, and more often very acidic range. This corresponds well to the low content of labile forms of calcium and magnesium and corresponds to the oligotrophy of the soils. The content of mobile forms of phosphorus, potassium and ammonium nitrogen is extremely high in suspended soils. The high content of nutrients in tropical suspended soils is enormously high compared to forest soils of other climatic zones and should be noted as their most important attributive characteristic. Taking into account the high accumulative-sorption capacity of suspended soils, the low content of heavy metals in them indicates the absence of modern aerotechnogenic pollution of all the studied ecosystems. Thus, we are talking about a certain deficiency of heavy metals in the eluvial ecosystem of the tropical rain forest. This once again confirms the hypothesis that the essence of epiphytic soil formation was and is that the biotic cycle breaks away from the ground and moves evolutionarily into the middle and upper tiers of the tropical rainforest ecosystem. Tree trunks in this context represent vertically and subhorizontally oriented phase interfaces, similar to parent materials, and epiphytic soils are analogues of peat accumulations with extremely high water-holding capacity, which is of extremely important environmental significance.

Preprint REVIEW | doi:10.20944/preprints202410.1140.v1

Exploring Diverse Biological Fertilizers (Micronutrients) for Sustainable Soil Fertility: A Review

Arfa Arfa, Kalsoom Akhtar, Ruksana Yousaf, Misbah Misbah, Namra Shahzadi, Hira Iqbal, Muhammad Usman Shahid

Subject: Biology And Life Sciences, Plant Sciences Keywords: sustainable; biological; fertilizers; micronutrients; soil fertility; microbial inoculants; soil health; Green Manure; organic matter; agriculture productivity

Online: 15 October 2024 (12:12:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.1064.v1

Oxidative Processes and Xenobiotic Metabolism in Plants: Mechanisms of Defense and Potential Therapeutic Implications

Caterina Vicidomini, Rosanna Palumbo, Maria Moccia, Giovanni N. Roviello

Subject: Biology And Life Sciences, Plant Sciences Keywords: plant metabolites; xenobiotic interactions; bioactive compounds; metabolomics; drug metabolism; human health

Online: 15 October 2024 (05:38:33 CEST)

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Plants are continuously exposed to environmental challenges, including pollutants, pesticides, and heavy metals, collectively termed xenobiotics. These substances induce oxidative stress by generating reactive oxygen species (ROS), which can damage cellular components such as lipids, proteins, and nucleic acids. To counteract this, plants have evolved complex metabolic pathways involving both enzymatic and non-enzymatic mechanisms to detoxify and process these harmful compounds. Oxidative stress in plants primarily arises from the overproduction of ROS, including hydrogen peroxide (H2O2), superoxide anions (O2•−), singlet oxygen (1O2), and hydroxyl radicals (•OH), by-products of metabolic activities such as photosynthesis and respiration. Exposure to xenobiotics significantly elevates ROS levels, causing cellular damage and disrupting metabolism. Plants employ a robust antioxidant defense system featuring enzymatic antioxidants like catalase (CAT), superoxide dismutase (SOD), and various peroxidases (POD). These enzymes collaborate to scavenge ROS, minimizing their detrimental effects. Additionally, plants produce numerous non-enzymatic antioxidants, such as flavonoids, phenolic acids, vitamins C and E, which neutralize ROS and regenerate other antioxidants, providing comprehensive protection against oxidative stress. Xenobiotic metabolism in plants involves three phases: Phase I (modification), Phase II (conjugation), and Phase III (compartmentalization), facilitated by enzymes like cytochrome P450 monooxygenases (CYPs), transferases such as glutathione S-transferases (GSTs), and ATP-binding cassette (ABC) transporters. Plant secondary metabolites, including alkaloids, terpenoids, and flavonoids, significantly contribute to detoxification and antioxidant defense. Understanding these oxidative processes and xenobiotic metabolism pathways in plants is crucial, as it offers insights into developing plant-based strategies to mitigate environmental pollution impacts and improve human health through enhanced detoxification and antioxidant mechanisms, as we discuss in this review.

Preprint ARTICLE | doi:10.20944/preprints202410.1049.v1

Comparative Analyses of the Complete Mitogenomes of Two Oxyria Species (Polygonaceae) Provide Insights into Understanding the Mitogenome Evolution Within the Family

Lijuan Li, Zhuo Jiang, Ye Xiong, Caleb Onoja Akogwu, Olutayo Mary Tolulope, Hao Zhou, Yanxia Sun, Hengchang Wang, Huajie Zhang

Subject: Biology And Life Sciences, Plant Sciences Keywords: Oxyria; Polygonaceae; Mitogenomes; Phylogenetic analysis; Repeat-mediated recombination

Online: 15 October 2024 (03:55:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.1103.v1

Analysis of Bacterial Community Changes in Apple Trees Treated with Biological Control Agent, Bacillus altitudinis KPB25, for Controlling Fire Blight

Na RA Lim, Hyun Gi Kong, Eon Jin Jo, Min Kyu Kang, Duck Hwan Park

Subject: Biology And Life Sciences, Plant Sciences Keywords: Erwinia amylovora; fire blight; Bacillus altitudinis; biological control; microbial community

Online: 14 October 2024 (21:23:50 CEST)

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This study aimed to investigate the potential of the antagonistic bacterium KPB25 (Bacillus altitudinis) as a biological control agent against fire blight by analyzing the changes in the epiphytic and endophytic bacterial communities of apple tree leaves following treatment. We used metagenomic analysis to examine the diversity and similarity of the bacterial communities before and after KPB25 treatment. The KPB25 treatment resulted in increased community richness and diversity in endophytic bacteria. Conversely, in epiphytic bacteria, community diversity decreased after treatment. Beta-diversity analysis revealed that the endophytic community formed distinct clusters following KPB25 treatment, indicating a significant shift in the community structure. However, the epiphytic community did not exhibit any clustering, and the KPB25 treatment did not result in substantial changes in the community structure. Relative abundance analysis of the endophytic and epiphytic communities highlighted that some bacterial families, which increased in abundance following KPB25 treatment, oxidized sugars into organic acids or produced antibiotics, potentially creating an environment that makes it difficult for Erwinia amylovora to survive when attempting to infect its host. These findings suggest that KPB25 interacts with certain microbial taxa within apple trees, contributing to the regulation and alteration of the microbial community in a manner that promotes an environment unfavorable for E. amylovora. Overall, KPB25 enhanced microbial groups within the residual bacterial community of apple leaves that contributed to fire blight suppression but did not significantly disrupt or alter the pre-existing community. Therefore, KPB25 has the potential to be a biological control agent for the management of fire blight in apple orchards. Its ability to interact with and modify the microbial community without causing significant disturbance further highlights its suitability for biological control.

Preprint REVIEW | doi:10.20944/preprints202410.0780.v1

Impact of Nanoparticles on Plant Growth, Development and Physiological Processes: A Comprehensive Review

Faryal Gohar, Usama Zafar Iqbal, Muhammad Bilal Khan, Fiza Rehman, Fatima Sahar Uraf Maryam, Muhammad Azmat, Sonia Munir, Hira Iqbal, Muhammad Usman Shahid

Subject: Biology And Life Sciences, Plant Sciences Keywords: Nanotechnology; microbial illness; pathway; productivity; translocation; interactions

Online: 10 October 2024 (09:52:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.0564.v1

ROS as Signaling Molecules to Initiate the Process of Plant Acclimatization to Abiotic Stress

Larisa Ivanovna Fedoreyeva

Subject: Biology And Life Sciences, Plant Sciences Keywords: reactive oxygen species; oxidative stress; abiotic stress; signaling wave; acclimatization

Online: 9 October 2024 (04:57:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.0591.v1

Role of the Foliar Endophyte Colletotrichum in the Resistance of Invasive Ageratina adenophora to Disease and Abiotic Stress

Ai-Ling Yang, Yu-Xuan Li, Zhao-Ying Zeng, Han-Bo Zhang

Subject: Biology And Life Sciences, Plant Sciences Keywords: Plant invasion; Ageratina Adenophora; Colletotrichum; Plant pathogen; Foliar endophytes; Drought and nutrient stress

Online: 9 October 2024 (04:31:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.0371.v1

Use and Overuse Leading the Conservation Issues of the Endangered Flagship Species Artemisia granatensis

Juan Lorite

Subject: Biology And Life Sciences, Plant Sciences Keywords: Medicinal plant species; Overexploitation; Threatened and Endemic species; Conservation; Sierra Nevada (SE Spain)

Online: 7 October 2024 (09:46:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202410.0242.v1

The Therapeutic Potential of Agarwood as an Antimicrobial and Anti-inflammatory Agent: A Scoping Review

Aswir Abd Rashed, Mohd Azerulazree Jamilan, Salina Abdul Rahman, Fatimah Diana Amin Nordin, Mohd Naeem Mohd Nawi

Subject: Biology And Life Sciences, Plant Sciences Keywords: Aquilaria spp.; antimicrobial; anti-inflammatory

Online: 4 October 2024 (08:13:11 CEST)

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Microorganisms such as bacteria, viruses and fungi are frequently the cause of infections. Antimicrobial agents, such as antibiotics, antivirals, and antifungals, are used to target and eliminate these infectious agents. Treating infections is crucial to prevent them from spreading, causing complications, or becoming chronic. On the other hand, inflammation is a natural response of the immune system to injury, infection, or irritation. While acute inflammation is a natural and protective process, chronic inflammation can contribute to various health issues, including autoimmune diseases, allergies, and chronic conditions like arthritis. Although herbal remedies have been used for centuries and can be effective in certain situations, it is crucial to use them with caution. Not all herbal remedies are supported by scientific evidence, and their safety and efficacy can vary. Thus, we conducted this review to determine the potential health benefits of agarwood as an antimicrobial and anti-inflammatory agent. Original articles were searched in three databases (PubMed, Scopus and Google Scholar) from the year 2013 to 2023 using the Medical subject heading (MeSH) terms “antimicrobial” and/or “anti-inflammatory”, crossed with the term “agarwood”. Synonyms and relevant search terms were also searched. The most studied agarwood for antimicrobial and anti-inflammatory agents is Aquilaria sinensis. Some studies have shown its potential application as a biopesticide for mosquito control and as a potent inhibitor for fungi, including Lasiodiplodia theobromae, Fusarium oxysporum, and Candida albicans. Moreover, it is capable of inhibiting Bacillus subtilis and Staphylococcus aureus activities. In conclusion, agarwood and its isolates are worthy of further biomedical investigation and could be developed as potential candidates for the treatment or prevention of various microbial and inflammatory diseases. This review reveals that despite the absence of clinical trials, agarwood exhibits antimicrobial and anti-inflammatory properties.

Preprint ARTICLE | doi:10.20944/preprints202410.0293.v1

Genome-Wide Characterization and Transcriptional Analyses of the Calmodulin and Calmodulin-Like Protein Family in Canavalia rosea Provide Insight into Their Potential Roles in Abiotic Stress Tolerance

Qianqian Ding, Zengwang Huang, Zhengfeng Wang, Shuguang Jian, Mei Zhang

Subject: Biology And Life Sciences, Plant Sciences Keywords: Calmodulin; Calmodulin-like protein; Ecological adaptability; Yeast heterogeneous expression; Canavalia rosea

Online: 4 October 2024 (08:10:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202410.0111.v1

Exploration of Candidate Korean Native Poaceae Plants for Breeding New Varieties as Garden Materials in the New Climate Regime

Sang Heon Kim, Wonwoo Cho

Subject: Biology And Life Sciences, Plant Sciences Keywords: garden plants; model plants; molecular breeding; native plants; Poaceae; ornamental plants

Online: 2 October 2024 (11:58:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0176.v2

Organizing the Chaos: Novel Insights into the Regulation of Z-3-Hexenal Production in Damaged Maize Leaves

Samantha Selman, Marie Engelberth, Jurgen Engelberth

Subject: Biology And Life Sciences, Plant Sciences Keywords: green leaf volatiles; calcium; hydrophobic cluster; biosynthesis; fatty acids; hexenal

Online: 2 October 2024 (09:43:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2344.v1

Natural Ginger Extract Versus Curcumin: Who Has Better Antioxidant and Anti-Inflammatory Properties for Wound Healing?

Li Sun, Long Chen, Jun Huang, Peikun Xin, Qiming Yang, Conglai Zhou, XiaoLi You

Subject: Biology And Life Sciences, Plant Sciences Keywords: ginger extract; curcumin; antioxidant; anti-inflammatory; wound healing

Online: 30 September 2024 (09:58:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2387.v1

Evaluation of the Presence, Frequency, and Distribution Range of Vegetal Invasive Alien Species in an Urban Area of Southern Spain

Herminia García-Mozo

Subject: Biology And Life Sciences, Plant Sciences Keywords: Plant alien species, invasive species, biodiversity conservation, plant ecology

Online: 30 September 2024 (09:43:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2240.v1

Genome-Wide Identification and Characterization of bHLH Gene Family in Hevea brasiliensis

Zheng Wang, Yuan Yuan, Fazal Rehman, Xin Wang, Tingkai Wu, Zhi Deng, Han Cheng

Subject: Biology And Life Sciences, Plant Sciences Keywords: bHLH transcription factor; Rubber tree; Cis-element; Expression analysis; Transcriptome; Abiotic stress; RT-qPCR; Gene structure; Subcellular localization

Online: 30 September 2024 (07:31:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.2087.v1

Evaluation of Brachypodium spp. System Model Against Fusarium poae

Florencia Arroyo, Mauro Martínez, Agustín Arata, MARIA VIRGINIA MORENO, Marie Dufresne, Sebastian Stenglein, María Inés Dinolfo

Subject: Biology And Life Sciences, Plant Sciences Keywords: plant-pathogen interactions, Fusarium poae, disease severity, Brachypodium spp

Online: 26 September 2024 (07:41:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1998.v1

Detection of Apple Proliferation Disease by Hyperspectral Sensors and Machine Learning Based Image Analysis

Uwe Knauer, Sebastian Warnemünde, Patrick Menz, Bonito Steffen Thielert, Lauritz Klein, Katharina Holstein, Miriam Runne, Wolfgang Jarausch

Subject: Biology And Life Sciences, Plant Sciences Keywords: hyperspectral; random forest; machine learning; apple; ‘ Candidatus Phytoplasma mali’ ; disease monitoring

Online: 25 September 2024 (12:16:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1860.v1

Impact of Mutations in Soybean Oleate and Linoleate Desaturase Genes on the Germinability of Seed from heat-Stressed Plants at the Anthesis Stage

Johnson O Toyinbo, Gautam Saripalli, Hrishikesh P Ingole, Zachary Jones, Salman Naveed, Enoch Noh, Sruthi Narayanan, Sachin Rustgi

Subject: Biology And Life Sciences, Plant Sciences Keywords: Soybean; heat stress; fatty acid desaturase mutants; expression analysis; germination

Online: 25 September 2024 (11:38:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1730.v1

Microsatellite Markers Determine the Genetic Structure and Diversity of Landraces of Quinoa from Ayacucho, Peru

Germán De la Cruz, Carla L. Saldaña, Francisco Menéndez, Edgar Neyra, Carlos I. Arbizu

Subject: Biology And Life Sciences, Plant Sciences Keywords: Andean crop; molecular markers; SSR; germplasm

Online: 23 September 2024 (08:55:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1057.v1

Improving Crops Using Omics Databases of Arabidopsis (Arabidopsis Thaliana) and Other Model Plants

Andrés S. Ortiz-Morazán, Marcela María Moncada

Subject: Biology And Life Sciences, Plant Sciences Keywords: Arabidopsis (Arabidopsis thaliana); Bioinformatics; Genetic improvement; Omics databases; Crops

Online: 13 September 2024 (14:51:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0833.v1

Exploring Lignin Biosynthesis Genes in Rice: Evolution, Functions, and Expressions

Munsif Ali Shad, Xukai Li, Muhammad Junaid Rao, Zixuan Luo, Xianlong Li, Aamir Ali, Lingqiang Wang

Subject: Biology And Life Sciences, Plant Sciences Keywords: rice; lignin monomers; gene families; cell wall; gene expression

Online: 11 September 2024 (12:32:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0772.v1

Commercialized “Smudge Sticks” Used as Incense in the Netherlands: An Inventory of Plants and Trends behind a New Age Fashion

Isabela Pombo Geertsma, Berber E. Zandstra, Anastasia Stefanaki, Tinde R. van Andel

Subject: Biology And Life Sciences, Plant Sciences Keywords: Ethnobotany; cultural appropriation; Native American; Europe; California; United States; ritual plants; Neo Paganism; Wicca; witchcraft

Online: 11 September 2024 (03:36:54 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0710.v1

Nutritional Values of Wheat and the Roles and Functions of Its Compositions in Health

Md. Zaherul Islam, Mohammad Nur Alam, Mohammad Mokhlesur Rahman, Mohammad Zaherul Islam, Aminur Rahman

Subject: Biology And Life Sciences, Plant Sciences Keywords: Gluten proteins; Nutritional quality; Vitamins and minerals; Wheat grain

Online: 9 September 2024 (16:45:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0683.v1

Comparative Evaluation of the Chemical Components and Anti-inflammatory Potential of Yellow- and Blue-Flowered Meconopsis Species: M. integrifolia and M. betonicifoliaCitation: To Be Added by Editorial Staff during Production

Peizhao Cheng, Ruixi Gan, Cong Wang, Qian Xu, Kelsang Norbu, Feng Zhou, Sixin Kong, Zhuoma Jia, Dawa Jiabu, Xin Feng, Junsong Wang

Subject: Biology And Life Sciences, Plant Sciences Keywords: Meconopsis; Meconopsis integrifolia; Meconopsis betonicifolia; Anti-inflammation; Metabolomics; Traditional Tibetan medicine; Chemical composition

Online: 9 September 2024 (11:51:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0615.v1

Brassica rapa BrICE1 and BrICE2 Positively Regulate the Cold Tolerance via CBFs and ROS Pathways, Balancing Growth and Defense in Transgenic Arabidopsis

Wangze Wu, Haobo Yang, Peng Xing, Guoting Zhu, Xueyan Han, Mei Xue, Guotai Min, Haijun Ding, Guofan Wu, Zigang Liu

Subject: Biology And Life Sciences, Plant Sciences Keywords: BrICE1 and BrICE2; freezing tolerance; phylogenetic tree; CBF pathway; Brassica rapa

Online: 9 September 2024 (08:42:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0513.v1

Susceptibility of different species of Aesculus L. to the chestnut miner moth Cameraria ohridella Deschka & Dimić: chemical composition and morphological features of leaves

Liliya R. Bogoutdinova, Olga V. Shelepova, Ludmila N. Konovalova, Oleg B. Tkachenko, Alexander A. Gulevich, Ekaterina N. Baranova, Irina V. Mitrofanova

Subject: Biology And Life Sciences, Plant Sciences Keywords: Cameraria ohridella; Aesculus spp.; infestation patterns; leaf miner; leaves; organic component analysis

Online: 6 September 2024 (09:40:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0153.v1

Selection of Urban Trees to Enhance Pollinator Food Resources: Comprehensive Consideration of Various Factors for Productivity Comparison

Sung-Joon Na, Ji-Min Park, Young-ki Kim

Subject: Biology And Life Sciences, Plant Sciences Keywords: unban tree; floral resource; availability; sugar yield; amino acid composition

Online: 3 September 2024 (11:54:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0176.v1

Organizing the Chaos: Novel Insights into the Regulation of Z-3-Hexenal Production in Damaged Maize Leaves

Samantha Selman, Marie Engelberth, Jurgen Engelberth

Subject: Biology And Life Sciences, Plant Sciences Keywords: green leaf volatiles; calcium; hydrophobic cluster; biosynthesis; fatty acids; hexenal

Online: 3 September 2024 (08:08:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0061.v1

Synthesis, Herbicidal Activity, and Molecular Mode of Action Evaluation of Novel Quinazolinone−Phenoxypropionate Hybrids Containing a Diester Moiety

Shumin Wang, Na Li, Shibo Han, Shuyue Fu, Ke Chen, Lusha Ji, Wenjing Cheng, Kang Lei

Subject: Biology And Life Sciences, Plant Sciences Keywords: quinazolinone; aryloxyphenoxypropionate; diester; ACCase; herbicides

Online: 2 September 2024 (09:02:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.2272.v1

Diversity and Ecological Functions of Anthocyanins

Nele Grünig, Jakob Maximilian Horz, Boas Pucker

Subject: Biology And Life Sciences, Plant Sciences Keywords: anthocyanin modification; flavonoids; specialized plant metabolism; pigmentation; transcriptional regulation; ecological function; metabolic diversity

Online: 30 August 2024 (15:41:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.2093.v1

The WRKY Family Transcription Factor GmWRKY72 Represses Glyceollin Phytoalexin Biosynthesis in Soybean

Jie Lin, Ivan Monsalvo, Hyejung Kwon, Sarah Pullano, Nik Kovinich

Subject: Biology And Life Sciences, Plant Sciences Keywords: Glycine max; transcription factor; transcriptional repressor; phytoalexin; glyceollin

Online: 29 August 2024 (09:34:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1969.v1

Complete Chloroplast Genome Sequences of Three Cleomaceae Species in China: Comparative and Phylogenetic Analysis

Dawei Shi, Ruijia Zhang, Lejia Zhao, Youyan Li

Subject: Biology And Life Sciences, Plant Sciences Keywords: Cleomaceae; Chloroplast genome; Comparative Genomics; Phylogenetic Relationships

Online: 28 August 2024 (02:56:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202408.1853.v1

Comprehensive Understanding of Recent Molecular and Physio-Biochemical Mechanisms in the Response of Tomatoes to Low Temperature Stress

Kwanuk Lee, Hunseung Kang

Subject: Biology And Life Sciences, Plant Sciences Keywords: low temperature (LT) response; cold stress; morphological and physiological traits; biochemical changes; C-repeat binding factor (CBF); RNA and DNA methylation; epigenetic regulations; climate change

Online: 27 August 2024 (11:54:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1706.v1

Insight into Romanian Wild-Grown Heracleum sphondylium: Development of a New Phytocarrier Based on Silver Nanoparticles with Antioxidant, Antimicrobial and Cytotoxicity Potential

Adina-Elena Segneanu, Gabriela Vlase, Titus Vlase, Ludovic Everard Bejenaru, George Dan Mogoşanu, Gabriela Buema, Maria Viorica Ciocîlteu, Cornelia Bejenaru

Subject: Biology And Life Sciences, Plant Sciences Keywords: Heracleum sphondylium; silver nanoparticles; phytocomplex; secondary metabolites; antioxidant potential; antimicrobial screening; in vitro cytotoxicity ;

Online: 27 August 2024 (02:12:13 CEST)

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Background: Heracleum sphondylium, a medicinal plant used in Romanian ethnopharmacology, proved to have a remarkable biological activity. The escalating concerns surrounding antimicrobial resistance led to a special attention to new efficient antimicrobial agents based on medicinal plants and nanotechnology. We report the preparation of a novel, simple phytocarrier that harnesses the bioactive properties of H. sphondylium and silver nanoparticles (HS-Ag system). Methods: The H. sphondylium low-metabolic profile was determined through gas chromatography–mass spectrometry and electrospray ionization–quadrupole time-of-flight–mass spectrometry. The morphostructural properties of the innovative phytocarrier were analyzed by X-ray diffraction, Fourier-transform infrared spectroscopy, Raman spectroscopy, dynamic light scattering, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The antioxidant activity was evaluated using total phenolic content, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) in vitro assays. The antimicrobial activity screening against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, and Escherichia coli was conducted using the agar well diffusion method. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay estimated the in vitro potential cytotoxicity on normal human dermal fibroblasts (NHDF) and cervical cancer (HeLa) cells. Results: A total of 88 biomolecules were detected, such as terpenoids, flavonoids, phenolic acids, coumarins, phenylpropanoids, iridoids, amino acids, phytosterols, fatty acids. HS-Ag phytocarrier heightened efficacy in suppressing the growth of all tested bacterial strains compared to H. sphondylium and exhibited a significant inhibition of HeLa cell viability. Conclusions: The new HS-Ag phytocarrier system holds promise for a wide range of medical applications. The data confirms the capacity to augment pertinent theoretical understanding in the innovative field of antimicrobial agents.

Preprint ARTICLE | doi:10.20944/preprints202408.1747.v1

Transplantation of Mature Argan Trees (Argania spinosa L. Skeels): Recovery Rate Optimization

Hamza El Ghazali, Lahoussaine Baâmal, Nour-Eddine Benaoda Tlemçani, Salma Daoud, Harrouni Moulay Cherif

Subject: Biology And Life Sciences, Plant Sciences Keywords: Argan tree; shoot pruning; root pruning; watering; transplantation; survival

Online: 23 August 2024 (17:16:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1661.v1

Four New Families of Arbuscular Mycorrhizal Fungi within the Order Glomerales

Gladstone Alves da Silva, Daniele Magna de Azevedo Assis, Ewald Sieverding, Fritz Oehl

Subject: Biology And Life Sciences, Plant Sciences Keywords: classification; systematics; taxonomy; arbuscular mycorrhizal fungi

Online: 23 August 2024 (04:08:27 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1650.v1

Optimizing Productivity of Tomato (Solanum lycopersicum L.) through Integrated Nutrient Sources and Inoculation with Arbuscular Mycorrhizal Fungi and Azospirillum spp.

Lowell Bernados, Joven Espineli, Julieta Anarna, Nelly Aggangan

Subject: Biology And Life Sciences, Plant Sciences Keywords: integrated nutrient management; arbuscular mycorrhizal fungi; Azospirillum; vermicompost; tomato

Online: 22 August 2024 (16:34:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1580.v1

Growth, Evapotranspiration, Gas Exchange and Ch a Fluorescence of Ipê‐Rosa Seedlings at Different Levels of Water Replacement

Kalisto Natam Carneiro Silva, Andréa Carvalho da Silva, Daniela Roberta Borella, Samuel Silva Carneiro, Leonardo Martins Moura dos Santos, Matheus Caneles Batista Jorge, Beatriz Feltrin Magosso, Mariana Pizzatto, Adilson Pacheco de Souza

Subject: Biology And Life Sciences, Plant Sciences Keywords: Handroanthus impetiginosus (Mart. ex DC.) Mattos; photosynthesis; Chl a fluorescence; water sensitivity

Online: 22 August 2024 (12:15:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1531.v1

R2R3 MYB Transcription Factor GhMYB201 Promotes Cotton Fiber Elongation via Cell Wall Loosening and Very-Long-Chain Fatty Acid Synthesis

Qingwei Suo, Nianjuan Fang, Jianyan Zeng, Fulin Yan, Xi Zhu, Yi Wang, Wanting Yu, Junmin Chen, Aimin Liang, Yaohua Li, Jie Kong, Yuehua Xiao

Subject: Biology And Life Sciences, Plant Sciences Keywords: Gossypium hirsutum; R2R3 MYB transcription factor; fiber elongation; cell wall loosening; KCS

Online: 22 August 2024 (04:09:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1564.v1

Inheritance of the Flesh Color and Shape of the Tuberous Root of Sweet Potato (Ipomoea batatas [L.] Lam.)

Alfredo Morales, Iván Javier Pastrana Vargas, Dania Rodríguez Del-Sol, Orelvis Portal Villafaña, Yoel Beovides García, Yuniel Rodríguez García, Alay Jiménez Medina, Yusbiel León Valdivies, Vaniert Ventura Chávez

Subject: Biology And Life Sciences, Plant Sciences Keywords: breeding; crosses; dominance; flesh color; inheritance; segregation

Online: 21 August 2024 (11:05:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1539.v1

µCT Imaging of Wheat Spikes Reveals Effect of Chronic Ozone on Individual Grains

Clare Brewster, Fiona Corke, Candida Nibau, Nathalie Fenner, Felicity Hayes

Subject: Biology And Life Sciences, Plant Sciences Keywords: Triticum aestivum L.; synthetic wheat; ozone stress; µ; CT digital imaging; grain number; grain morphology

Online: 21 August 2024 (08:52:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1407.v1

Translational Regulation of Duplicated Gene Expression Evolution in Allopolyploid Cotton

Guiling Fu, Haotian Luo, Juqing Jia, Mingming Hou, Guanjing Hu

Subject: Biology And Life Sciences, Plant Sciences Keywords: Polyploidization; Translatome; Transcriptome; Cotton; Ribo-seq; Translation Efficiency

Online: 20 August 2024 (04:38:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1148.v1

The Effects of Auxin Transport Inhibition on the Formation of Various Leaf and Vein Patterns

Carol Wenzel, David Holloway, Jim Mattsson

Subject: Biology And Life Sciences, Plant Sciences Keywords: convergent points; leaf complexity; polar auxin transport (PAT); venation patterning

Online: 15 August 2024 (14:32:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.1141.v1

A Gwas Approach to Identify Novel Major-Effect Qtls for End-Use Quality Traits in Soft Red Winter Wheat

Madhav Subedi, John White Bagwell, Benjamin Lopez, Byung-Kee Baik, Md Ali Babar, Mohamed Mergoum

Subject: Biology And Life Sciences, Plant Sciences Keywords: Quality, kernel, flour, protein, SRC, cookie diameter, softness, genes, QTL

Online: 15 August 2024 (10:45:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0833.v1

Populus trichocarpa EXPA6 Increases Salt Sensitivity by Facilitating Radial and Longitudinal Transport of Sodium Ions in Poplars

Zhe Liu, Kexin Yin, Ying Zhang, Caixia Yan, Ziyan Zhao, Jing Li, Yi Liu, Bing Feng, Rui Zhao, Jian Liu, Kaiyue Dong, Jun Yao, Nan Zhao, Xiaoyang Zhou, Shaoliang Chen

Subject: Biology And Life Sciences, Plant Sciences Keywords: Populus; expansin; cell wall loosening; comparative contractility, comparative extensibility, Na+ fluxes; photosynthesis; SOD; POD; CAT

Online: 12 August 2024 (14:52:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0689.v1

Exploration of the Commonalities and Specificities in Wheat Respond to Aluminum Toxicity and Low Phosphorus via Transcriptomics and Targeted Metabolomics Study

Daozhen Luo, Qing Li, Fei Pang, Wenjie Zhang, Yangrui Li, Yongxiu Xing, Dengfeng Dong

Subject: Biology And Life Sciences, Plant Sciences Keywords: wheat root; transcriptomic; metabolomic; Al tolerance; P efficiency

Online: 12 August 2024 (04:42:00 CEST)

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Aluminum (Al) toxicity and low phosphorus availability (LP) are the top two co-existing edaphic constraints limiting agriculture productivity in acid soils. Plants have evolved versatile mechanisms to cope with the two stresses alone or simultaneously. However, the specific and common molecular mechanisms, especially those on flavonoids and carbohydrate metabolism, are still unclear. Laboratory studies were conducted on two wheat genotypes, Fielder (Al-tolerant and P-efficient) and Ardito (Al-sensitive and P-inefficient), exposed to 50 μM Al and 2 μM Pi (LP) in hydroponic solutions. After 4 days of stress, wheat roots were analyzed via transcriptomic and targeted metabolomic techniques. A total of 2296 differentially expressed genes (DEGs, 1535 up and 761 down) under Al and 3029 DEGs (1591 up and 1438 down) under LP were identified in Fielder. Similarly, 4404 DEGs (3191 up and 1213 down) under Al and 1430 DEGs (1176 up and 254 down) under LP were identified in Ardito. The GO annotation analysis results that 4079 DEGs annotated to the metabolic processes term. These DEGs were significantly enriched in the phenylpropanoid, flavonoid, flavone and flavonol biosynthesis, and carbohydrate metabolism pathways by performing the KEGG enrichment analysis. The targeted metabolome analysis detected 19 flavonoids and 15 carbohydrate components in Fielder and Ardito under Al and LP. More responsive genes and metabolites were involved in flavonoid metabolism under LP than Al in the Fielder, while an opposite trend in the Ardito. In the carbohydrate metabolism pathway, the genes and metabolites were higher in Fielder than in Ardito. The combined transcriptome and metabolome analysis revealed that the flavonoid and carbohydrate genes and metabolites differed between Fielder and Ardito under Al and LP, which may be an important reason for Fielder’s high Al and LP resistance. The results of this study lay a foundation for pyramiding genes and breeding multi-resistance varieties.

Preprint ARTICLE | doi:10.20944/preprints202408.0626.v1

The Legacy Effect of Mountain Pine Beetle Outbreaks on Chemical and Anatomical Defenses of Surviving Lodgepole Pine Trees

Gigi Baker, Shiyang Zhao, Jennifer G Klutsch, Guncha Ishangulyyeva, Nadir Erbilgin

Subject: Biology And Life Sciences, Plant Sciences Keywords: carbohydrates; bark beetle outbreaks; lodgepole pine; terpene defenses; resin ducts

Online: 8 August 2024 (18:16:55 CEST)

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The recent mountain pine beetle outbreaks have caused widespread mortality among lodgepole pine trees in western North America, resulting in a reduced population of surviving trees. While previous studies have focused on the cascading impacts of these outbreaks on the physiology and growth of the surviving trees, there remains a need for a comprehensive study into the interactions among various physiological traits and the growth in post-outbreak stands. Specifically, the relationship between chemical (primarily terpenes) and anatomical (mainly resin ducts) defences, as well as the allocation of non-structural carbohydrates (NSCs) to support these defence modalities, is poorly understood. To address these gaps, we conducted a field survey of surviving lodgepole pine trees in post-mountain pine beetle outbreak stands in western Canada. Our retrospective analysis targeted to determine correlations between the post-outbreak concentrations of monoterpenes, diterpenes, and NSCs in the phloem, and the historical resin duct characteristics and growth traits before and after the outbreak. We detected strong correlations between post-outbreak concentrations of monoterpenes and historical resin duct characteristics, suggesting a possible link between these two defence modalities. Additionally, we found a positive relationship between NSCs and the total concentrations of monoterpenes and diterpenes, suggesting that NSCs likely influence the production of these terpenes in lodgepole pine. Furthermore, historical tree growth patterns showed strong positive correlations with many individual monoterpenes and diterpenes. Interestingly, while surviving trees had enhanced anatomical defences after the outbreak, their growth patterns did not vary before and after the outbreak conditions. The complexity of these relationships emphasizes the dynamics of post-outbreak stand dynamics and resource allocation in lodgepole pine forests, highlighting the need for further research. These findings contribute to a broader understanding of conifer defenses and their coordinated responses to forest insect outbreaks, with implications for forest management and conservation strategies.

Preprint ARTICLE | doi:10.20944/preprints202408.0590.v1

Exploring Senolytic and Senomorphic Properties of Medicinal Plants for Anti-Aging Therapies

Monika Imb, Zsolt Véghelyi, Michael Maurer, Harald Kühnel

Subject: Biology And Life Sciences, Plant Sciences Keywords: inflammation; herbal extracts; senolytics; senomorphics; chamomile; etoposide

Online: 8 August 2024 (08:37:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202405.1356.v2

Green Leaf Volatiles: A New Player in the Protection against Abiotic Stresses?

Jurgen Engelberth

Subject: Biology And Life Sciences, Plant Sciences Keywords: abiotic stress; airborne signal; green leaf volatiles; GLV; plant damage; plant protection; plant volatiles; structural integrity; volatile organic compounds; VOC

Online: 7 August 2024 (14:11:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0494.v1

Complete Chloroplast Genome Assembly of Garcinia indica (Thouars) Choisy: Comparative Genomics and Identification of Potential Barcode Markers for Kokum

Raju Balaji, Subbalakshmi Easwaran, Krishnamoorthy Devanathan, Md. Rizwanullah, Gupta Tanuja, Vikram Pal Gandhi

Subject: Biology And Life Sciences, Plant Sciences Keywords: Chloroplast genome; Clusiaceae; DNA barcoding; Garcinia indica; Kokum authentication; Malpighiales

Online: 7 August 2024 (08:38:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0287.v1

Effects of UV-B and UV-C Spectrum Supplementation on the Antioxidant Properties and Photosynthetic Activity of Lettuce Cultivars

Ernest Skowron, Magdalena Trojak, Ilona Pacak

Subject: Biology And Life Sciences, Plant Sciences Keywords: indoor farming; spectrum supplementation; ultraviolet light; antioxidant potential; lettuce

Online: 5 August 2024 (14:34:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0227.v1

Genotype-by-Environment Interaction and Stability of Canola (Brassica napus L) for Weed Suppression through Improved Interference

Asad (Md) Asaduzzaman, Hanwen Wu, Gregory Doran, Jim Pratley

Subject: Biology And Life Sciences, Plant Sciences Keywords: competition; neighbouring plant; root; root exudates; adaption

Online: 5 August 2024 (08:18:11 CEST)

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Canola (Brassica napus L) is a profitable grain crop for Australian growers. However, weeds remain a major constraint for its production. Chemical herbicides are used for weed control, but this tactic also leads to the evolution of herbicide resistance in different weed species. Suppression of weeds by crop competition and allelopathic mechanisms has been receiving significant attention. Here, the weed suppressive ability, and associated functional traits and stability of four selected canola genotypes (PAK85388-502, AV-OPAL, AV-GARNET and BAROSSA) were examined at different locations of NSW, Australia. Results showed that there were significant effects of canola genotypes and of genotypes by crop density interaction on weed growth. Among the tested genotypes, PAK85388-502 and AV-OPAL were the most weed suppressive and, at plant density of 10 plants/m2, they reduced weed biomass of wild radish, shepherd’s purse and annual ryegrass by more than 80%. No significant differences were found in the primary root lengths among canola varieties; however, plants of the allelopathic genotype PAK8538-502 exhibited a 35% increase in lateral root number relative to plants of non-allelopathic genotype BAROSSA. The analysis of variance revealed a significant influence of genotypes with PAK85388-502 and AV-OPAL performed the best across all the research sites. Results showed that canola genotypes PAK85388-502 and AV-OPAL were more weed suppressive than AV-GARNET and BAROSSA and may release specific bioactive compounds in their surroundings to suppress neighbouring weeds. This study provides valuable information that could be utilised in breeding programs to select weed suppressive varieties of canola in Australia. Thus, lateral root number could be a potential target trait for weed suppressive varieties. Additionally, other root architecture traits may contribute to the underground allelopathic interaction to provide a competitive advantage to the crop.

Preprint ARTICLE | doi:10.20944/preprints202408.0135.v1

Polyphenols Investigation, Antioxidant and Anticholinesterase Activity of Rosmarinus officinalis L. Species from Southwest Romania Flora

Ludovic Everard Bejenaru, Andrei Biţă, George Dan Mogoşanu, Adina-Elena Segneanu, Antonia Radu, Maria Viorica Ciocîlteu, Cornelia Bejenaru

Subject: Biology And Life Sciences, Plant Sciences Keywords: Rosmarinus officinalis; Romanian flora; polyphenols; UHPLC/MS quantification; antioxidant activity; anticholinesterase activity

Online: 2 August 2024 (10:36:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202408.0123.v1

Bio-Morphological, Reproductive and Ecological-Physiological Characteristics of Endangered Amberboa moschata (Asteraceae) and Some Aspects of Its Ex Situ Conservation

Janna Akopian, Anahit Ghukasyan, Araksya Elbakyan, Lora Martirosyan, Zhanna Hovakimyan

Subject: Biology And Life Sciences, Plant Sciences Keywords: Amberboa moschata; adaptive properties; ex situ conservation; chromosomes number; pollen fertility; Botanical Garden

Online: 2 August 2024 (03:48:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.2507.v1

Methods for Determination of Antimicrobial Activity of Essential Oils In Vitro – A Review

Radka Hulankova

Subject: Biology And Life Sciences, Plant Sciences Keywords: agar diffusion; agar dilution; antibacterial; biofilm; broth dilution; plant extracts; vapor phase

Online: 31 July 2024 (11:09:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2154.v1

Unraveling Evolutionary Dynamics: Comparative Analysis of Chloroplast Genome of Cleomella serrulata

Madelynn K. Vasquez, Emma K. Stock, Kaziah J. Terrell, Julian Ramirez, John A. Kyndt

Subject: Biology And Life Sciences, Plant Sciences Keywords: Cleomella; native plant; medicinal plant; chloroplast evolution; Nebraska; Rocky Mountain bee plant; stinking clover; phylogeny

Online: 26 July 2024 (13:01:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.2069.v1

FaNPR3 Members of the NPR1-Like Gene Family Negatively Modulate Strawberry Fruit Resistance against Colletotrichum acutatum

Victoria Súnico, José Javier Higuera, Francisco Amil-Ruiz, Isabel Arjona-Girona, Carlos J. López-Herrera, Juan Muñoz-Blanco, Ana María Maldonado-Alconada, José L. Caballero

Subject: Biology And Life Sciences, Plant Sciences Keywords: Fragaria ananassa; strawberry defense; strawberry resistance; Colletotrichum acutatum; FaNPR3; NPR-like genes; AtNPR3; AtNPR4; FaWRKY19; FaWRKY24

Online: 25 July 2024 (14:06:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1930.v1

Phytochemical Analysis on the Female Cone of Araucaria bidwillii Hook

Claudio Frezza, Alessandro Venditti, Ottavia Giampaoli, Chiara Dal Bosco, Marco Franceschin, Claudio Scintu, Fabio Sciubba, Mauro Serafini, Daniela De Vita, Fabio Attorre

Subject: Biology And Life Sciences, Plant Sciences Keywords: Araucaria bidwillii Hook; female cone; Central Italy; phytochemical analysis; chemophenetic analysis

Online: 24 July 2024 (12:03:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1708.v1

Permanent Stress Adaptation and Unexpected High Light Tolerance in the Shade-Adapted Chlamydomonas Priscuii

Devon Popson, Susanna D'Silva, Kaylie Wheeless, Rachael Morgan-Kiss

Subject: Biology And Life Sciences, Plant Sciences Keywords: photoinhibition; photo-acclimation; extremophile; cyclic electron flow; environmental change

Online: 22 July 2024 (11:50:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.1579.v1

Combined Genome-Wide Association Studies (GWAS) and Linkage Mapping Identifies Genomic Regions Associated with Seedling Root System Architecture (RSA) under Different Nitrogen Conditions in Wheat (Triticum aestivum L.)

Yulin Jia, Ninglu Xu, Jun Zhang, Kaiming Ren, Jinzhi Wu, Chunping Wang, Ming Huang, Youjun Li

Subject: Biology And Life Sciences, Plant Sciences Keywords: Keywords：Triticum aestivum L; root system architecture; N use efficiency; GWAS; linkage mapping; 660K SNP chip; DArT

Online: 19 July 2024 (08:37:45 CEST)

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The nutrient absorption and utilization efficiency in the roots of seedlings are beneficial for increasing crop yield. Creating marker assisted selection for wheat root traits can assist wheat breeders in choosing robust roots to maximize nutrient uptake. Exploring and identifying the effect of different nitrogen applications on RSA is of great significance for breeding nitrogen efficient wheat varieties. In this study, A total of 243 wheat varieties native to the Yellow and Huai Valley regions of China were utilized for genome-wide association studies (GWAS). Furthermore, a recombinant inbred line (RIL) population of 123 lines derived from the cross between Avocet and Chilero was utilized for linkage examination and confirmation of the hereditary loci distinguished by GWAS. Five RSA traits include the relative number of root tips (RNRT); relative total root length (RTRL); relative total root surface area (RTRS); relative total root volume (RTRV) and relative average root diameter (RARD) where evaluated under two N conditions—normal N (NN) supply and low N (LN)—to characterize the NUE of each accession. GWAS and linkage analysis were performed by integrating data from the wheat 660k single nucleotide polymorphism (SNP) chip and diversity arrays technology (DArT) to identify genetic loci associated with RSA. The results showed that based on the ratio of RSA related traits under two N supply conditions, a total of 598 SNP markers, which are significantly associated with RSA related traits, were detected at 160 genetic loci by GWAS. A total of 10 QTL loci related to RSA were dis-covered and identified by linkage mapping. In which, three significant RSA related Quantitative Trait Locus (QTL) were identified, namely qRtrl-3D, qRnrt-3D, and qRtrl-7D. The study has the potential to enhance the effectiveness of selecting root traits in wheat breeding programs, offering valuable insights into the genetic underpinnings of nitro-gen use efficiency (NUE) in wheat. This could lead to the creation of NUE wheat varieties by implementing focused breeding strategies.

Preprint ARTICLE | doi:10.20944/preprints202407.1442.v1

BSMV-Mediated Assays for Oat Functional Genomics

Emmanuel N. Annan, Barbara K. Keith, Akamjot Brar, William E. Dyer, Li Huang

Subject: Biology And Life Sciences, Plant Sciences Keywords: BSMV; VIGS; VOX; Avena fatua; Avena sativa; functional genomics

Online: 18 July 2024 (17:28:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.1424.v1

The Past, Present and Future of Plant Activator Targeting Salicylic Acid Signal Pathway

Misbah Naz, Dongqin Zhang, Kangcen Liao, Xulong Chen, Nazeer Ahmed, Jing-Jiang Zhou, Zhuo Chen

Subject: Biology And Life Sciences, Plant Sciences Keywords: plant activator; salicylic acid signal pathway; systemic acquired resistance; plant resistance; action mechanism

Online: 17 July 2024 (11:39:53 CEST)

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Plant activators have emerged as promising alternatives to conventional pesticides for crop disease manage-ment due to their unique mode of action. By priming the plant's innate immune system, these compounds can induce systemic acquired resistance against a broad spectrum of pathogens without directly inhibiting their proliferation. Key advantages of plant activators include prolonged defense activity, lower effective dosages, and negligible risk of developing pathogen resistance. Among the various defensive pathways tar-geted, the salicylic acid (SA) signaling cascade has been extensively explored, leading to the successful devel-opment of commercial activators like benzothiadiazole for widespread application in crop protection. While the action sites of many SA-targeting activators have been preliminarily mapped to different steps along the pathway, a comprehensive understanding of their precise mechanisms remains elusive. This review provides a historical perspective on plant activator development and outlines diverse screening strategies employed across multiple levels, from whole-plant bioassays to molecular and transgenic approaches. We expound on the intricate components, biological relevance, and regulatory circuits governing the SA pathway, while criti-cally examining the structural features, bioactivities, and proposed modes of action of classical activators such as benzothiadiazole derivatives, salicylic acid analogs, and other small molecules. Insights from field trials as-sessing their practical applicability are also discussed. Furthermore, we highlight the current status, challenges, and future prospects in the realm of SA-targeting activator development globally, with a focus on recent en-deavors in China. Collectively, this comprehensive review aims to synthesize existing knowledge and provide a roadmap for future research toward developing more potent and mechanistically understood plant activa-tors that fortify crop immunity against disease.

Preprint ARTICLE | doi:10.20944/preprints202407.1259.v1

Pectin Remodeling and Involvement of AtPME3 in the Parasitic Plant-Plant Interaction Arabidospis thaliana – Phelipanche ramosa

Cyril Grandjean, Christophe Veronesi, Christine Rusterucci, Charlotte Gautier, Yanis Maillot, Maïté Leschevin, Françoise Fournet, Jan Drouaud, Paulo Marcelo, Luciane Zabijak, Philippe Delavault, Philippe Simier, Sophie Bouton, Karine Pageau

Subject: Biology And Life Sciences, Plant Sciences Keywords: cell wall; pectin acetyl esterase; pectin methyl esterase; Pectin Remodeling Enzymes; Phelipanche ramosa-Arabidopsis thaliana

Online: 16 July 2024 (04:10:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1263.v2

Automated ISSR Fingerprinting is a Cost-effective way to assess Genetic Diversity and Taxonomic Differentiation – a Case Study on the Encephalartos eugene-maraisii Species Complex

Deanne Murphy, Nigel Paul Barker, Arnold Frisby

Subject: Biology And Life Sciences, Plant Sciences Keywords: cycad; ISSR; conservation genetics; species complex; developing countries; technological impediment

Online: 15 July 2024 (13:00:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0755.v1

A Terpenoid-Rich Fraction of Inula viscosa: Its Chemical Composition, Antioxidant Capacity, and Anticancerous Effects Against Human Lung Cancer Cells

Fatiha Seglab, Mazen Abou Assali, Thoraya AlYafei, Hassan Hassan, Diana C.G.A Pinto, Safa Baydoun, Asma A. Al Thani, Abdullah A. Shaito

Subject: Biology And Life Sciences, Plant Sciences Keywords: antioxidant capacity; Inula viscosa; Dittrichia viscosa; A549 cells; cytotoxic activity; terpenoids; dichloromethane

Online: 10 July 2024 (11:24:53 CEST)

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Inula viscosa is a widely used plant in traditional Mediterranean and Middle Eastern medicine for various illnesses. I. viscosa has been shown to have anticancerous effects against various cancers, but its effects against lung cancer have been under limited investigation. At the same time, I. viscosa is rich in terpenoids whose anti-lung cancer effects have been poorly investigated. This study aimed to examine the potential anticancerous properties of methanolic and aqueous extracts of stems and leaves of I. viscosa and its terpenoid-rich fraction against human lung cancer A549 cells. Results showed a high radical scavenging capacity and polyphenol and flavonoid contents of I. viscosa methanolic rather than aqueous extracts. In addition, leaves methanolic extracts (IVLM) showed the highest reduction in viability of A549 cells among all the extracts. IVLM also reduced the viability of human ovarian SK-OV-3, breast MCF-7, liver HepG2, and colorectal HCT116 cancer cells. A terpenoid-rich I. viscosa fraction (IVL DCM), prepared by liquid-liquid separation of IVLM in dichloromethane (DCM), displayed a substantial reduction in the viability of A549 cells (IC₅₀= 27.8 ± 1.5 µg/mL at 48 h) and the panel of tested cancerous cell lines but was not cytotoxic to normal human embryonic fibroblasts (HDFn). The assessment of IVL DCM phytochemical constituents using GC-MS analysis revealed 21 metabolites, highlighting an enrichment in terpenoids, such as lupeol and its derivatives, caryophyllene oxide, betulin, and isopulegol, known to exhibit proapoptotic, and antimetastatic functions. IVL DCM also showed robust antioxidant capacity and decent polyphenol and flavonoid contents. Furthermore, Western blotting analysis indicated that IVL DCM reduced proliferation (reduction of proliferation marker Ki67 and induction of proliferation inhibitor proteins P21 and P27), contaminant with P38 MAP kinase activation, and induced the intrinsic apoptotic pathway (P53/BCL2/BAX/ Caspase3/PARP) in A549 cells. IVL DCM also reduced the migration of A549 cells, potentially by reducing FAK activation. Future identification of anticancer metabolites of IVL DCM, especially terpenoids, is recommended. These data place I. viscosa as a new resource of herbal anticancer agents.

Preprint ARTICLE | doi:10.20944/preprints202407.0826.v1

Potential Regulation of Iron Metabolism Gene Expression by DOF Transcription Factors in Dendrocalamus latiflorus

Peng-Kai Zhu, Mei-Xia Lin, Mei-Yin Zeng, Yu Tang, Xin-Rui Li, Tian-You He, Yu-Shan Zheng, Ling-Yan Chen

Subject: Biology And Life Sciences, Plant Sciences Keywords: DOF transcription factors; Iron Metabolism Related Genes; gene regulation; gene expression; Dendrocalamus latiflorus

Online: 10 July 2024 (10:14:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0715.v1

Immune Responses of Mango Callus Infected by Agrobacterium tumefaciens Inhibited Transformation

Haiyan Shu, Zilhas Ahmed Jewel, Luqiong He, Qing Wei, Rulin Zhan, Shenghe Chang

Subject: Biology And Life Sciences, Plant Sciences Keywords: Mango callus; Agrobacterium(T3SS) gene; Reactive oxygen species(ROS)gene; Gallic acid synthesis gene

Online: 9 July 2024 (08:46:29 CEST)

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Mango industry played important roles in the economy of tropical and subtropical regions in China. Embryo abortion, flesh rotten, pericarp stain and so on always result in heavy loss. Transgenic breeding is a potential way for solving these problems. However, dependable protocol for mango transgene has not been reported until now. In this research, we found that after mango embryogenic callus were infected by Agrobacterium, most callus necrotized and died. No seedling was differentiated from the survived callus. Contents of 106 compounds in mango callus infected by Agrobacterium were found significantly different from those in control. The top 7 up-regulated compounds were gallic acid, phosphoenolpyruvic acid, dihydrotricetin, ubiquinone, dihydrokaempferol, D-erythrose-4-phosphate and cyanidin. The expressions of ROS-related genes, gallic acid synthesis genes, and defense related genes in callus infected by Agrobacterium were significantly higher than those in control. The growth of Agrobacterium on culturing plate pipetting gallic acid was inhibited. Mango callus infected by Agrobacterium transformed with type III secretion system gene cluster (T3SS) and effector gene AvrPto isolated from Pseudomonas syringae pv. tomato strain DC3000 brown and necrotize slower than those infected by Agrobacterium transformed with net vector (NV). The contents of ROS in callus in-fected by Agrobacterium (T3SS-AvrPto) were less than those in callus infected by Agrobacterium (NV). The expressions of genes participated in immune responses in mango callus infected by Agrobacterium (T3SS-AvrPto) were also less than those in mango callus infected by Agrobacterium (NV). The transformation rate of mango callus infected with Agrobacterium (T3SS-AvrPto-gfp) was 1.6 %. No callus infected with Agrobacterium (NV-gfp) was transformed successfully. The efficiency of mango transformation can be improved by constructing Agrobacterium strains harboring type III secretion system gene cluster and its effector gene.

Preprint ARTICLE | doi:10.20944/preprints202407.0713.v1

Genomic Selection for Growth and Wood Traits in Castanopsis hystrix

Weihua Zhang, Ruiyan Wei, Yuanzhen Lin

Subject: Biology And Life Sciences, Plant Sciences Keywords: Castanopsis hystrix; genomic selection; growth trait; wood trait; early selection; SNP

Online: 9 July 2024 (08:45:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0524.v1

The REPLUMLESS Transcription Factor Controls the Expression of the RECEPTOR-LIKE CYTOPLASMIC KINASE VI_A2 Gene Involved in Shoot and Fruit Patterning of Arabidopsis thaliana

Erzsébet Kenesi, Orsolya Beöthy-Fehér, Réka Szőllősi, Ildikó Domonkos, Ildikó Valkai, Attila Fehér

Subject: Biology And Life Sciences, Plant Sciences Keywords: phyllotaxis; stem thickness; BELLRINGER transcription factor; replum; vascular bundles; ROP GTPase-activated kinase

Online: 9 July 2024 (06:03:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0483.v1

Research Progress on the Mechanisms of Terrestrial Plant Uptake, Transport, and Growth Inhibition Responses to Micro (nano) Plastics

Shuxuan Yin

Subject: Biology And Life Sciences, Plant Sciences Keywords: micro (nano) plastics; plant growth; absorption; transposition pathways

Online: 8 July 2024 (13:12:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0560.v1

Relaxing Effect of 8-Methoxycirsilineol Extracted (Larrea tridentata) by Potassium Channels Depend-Voltage Activation on Vascular Smooth Muscle from Guinea Pig

Paola Algara-Suarez, Martha Patricia Navarro-Huerta, Marco Martín González-Chávez, Xochitl Ordaz-Ruiz, Aldo Azmar Rodríguez-Menchaca, Iván Arael Aréchiga-Figueroa, María Dolores Brito-Orta, Juan Roberto Valle-Aguilera, Ricardo Espinosa-Tanguma

Subject: Biology And Life Sciences, Plant Sciences Keywords: Larrea tridentata, 8-Methoxycirsilineol, vascular smooth muscle, Flavone, Potassium Channels

Online: 8 July 2024 (10:05:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0608.v1

Exploring S-Benzyl-L-Cysteine as an O-acetylserine(thiol) Ly-Ase Inhibitor: Effects on Growth, Photosynthesis, and Oxidative Balance of Ipomoea grandifolia Plants

Danielly Caroline Inacio Martarello, Luiz Grizza, Marcela de Paiva Foletto-Felipe, Ana Paula da Silva Mendonça, Renato Polimeni Constantin, Ana Paula Ferro, Wanderley Dantas dos Santos, Rodrigo Polimeni Constantin, Rogerio Marchiosi, Osvaldo Ferrarese-Filho

Subject: Biology And Life Sciences, Plant Sciences Keywords: L-cysteine; herbicides; OAS-TL; sulfur assimilation pathway; weeds

Online: 8 July 2024 (10:02:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0465.v1

Photosystem I: A Paradigm for Understanding Biological Environmental Adaptation Mechanisms

Lirong Tian, Jing-Hua Chen

Subject: Biology And Life Sciences, Plant Sciences Keywords: photosystem I; LHCI; cyanobacteria; algae; environmental adaptation

Online: 5 July 2024 (02:49:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0364.v1

TheEffect of Different Soil Parameters on the Germination Of New Grass Species Applicable to Urban Environments. Germination of Festuca Taxa Under Greenhouse Conditions

Tünde Szabó-Szöllősi, Éva Horváthné Baracsi, Péter Csontos, László Papp, Szilvia Kisvarga, László Orlóci, Judit Házi, Zoltán Kende, Dénes Saláta, Ákos Tarnawa, Katalin Rusvai, Károly Penksza

Subject: Biology And Life Sciences, Plant Sciences Keywords: ; germination; Poaceae; soil; sandy grassland; taxonomic clarification

Online: 4 July 2024 (09:13:51 CEST)

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This survey is part of a Hungarian Research OTKA project that examines the vegetation of sandy grasslands along the Danube. During this study, two grass species, Festuca wagneri and Festuca tomanii, were identified as potentially suitable for urban planting and turf establishment based on preliminary research. Our aim was to determine the germination success of seeds from aesthetically selected individuals and to identify the soil types on which they germinate most effectively. This information could be crucial for future cultivation efforts. From the collected Festuca individuals, were analyzed 30 specimens of each taxon under garden conditions and selected the indi-viduals for germination. The Festuca tomanii individuals were uniform, so we selected only 5 individuals. The Festuca wagneri individuals were categorized into three groups: upright, spreading, and low types. It was assumed that Festuca species seeds would germinate better in sandy soils. To test this, seeds from ten Festuca wagneri and five Festuca tomanii individuals, selected based on aesthetic criteria, were sown in six different substrates: a sand-peat mixture, sand, coconut fiber, peat, a coconut fiber-sand mixture, and native sandy soil. Contrary to expectations, the growth and germination rates of seeds sown in peat and coconut fiber substrates were higher than those in native sandy soil. These results suggest that Festuca seeds germinate better on substrates resembling dead plant debris with a peat-like structure or on the surface of live mosses, rather than on bare sand. Among the examined individuals, the seeds from the spreading Festuca wagneri group exhibited the highest germination rate, making this group particularly suitable for urban environments. Addi-tionally, one of the upright Festuca wagneri individuals showed the highest growth rate and should also be considered for urban planting. In contrast, despite their uniform appearance, the Festuca tomanii individuals did not demonstrate similar germination trends. In fact, the seeds from two clumps did not germinate at all, indicating that further selection is necessary.

Preprint ARTICLE | doi:10.20944/preprints202407.0177.v1

Bacterial Communities Associated with the Leaves and the Roots of salt marsh plants of Daphne, Alabama, USA

Aqsa Majeed, Jinbao Liu, Adelle J Knight, Karolina M. Pajerowska-Mukhtar, M. Shahid Mukhtar

Subject: Biology And Life Sciences, Plant Sciences Keywords: Metagenome; 16S; coastal salt marsh; salt stress; plants

Online: 2 July 2024 (14:49:01 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202407.0192.v1

Plant Obliviousness to Plant Consciousness Through Knowledge Gap Bridging

Olaoluwa AJAYI, Vincent H. OGWUGWA, Mohammadi DIANDA

Subject: Biology And Life Sciences, Plant Sciences Keywords: Plant knowledge; Plant oblivion; Sustainability; Plant perception; Plant consciousness

Online: 2 July 2024 (11:13:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0197.v1

Genome-Wide Association Analysis Identifies Candidate Loci for the Callus Induction in Rice (Oryza sativa L.)

Wintai Kamolsukyeunyong, Yeetoh Dabbhadatta, Aornpilin Jaiprasert, Burin Thunnom, Wasin Poncheewin, Samart Wanchana, Vinitchan Ruanjaichon, Theerayut Toojinda, Parichart Burns

Subject: Biology And Life Sciences, Plant Sciences Keywords: Rice; GWAS; QTLs; callus induction; gene

Online: 2 July 2024 (11:10:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0193.v1

Mapping the Influence of Light Intensity on the Transgenerational Genetic Architecture of Arabidopsis thaliana

Jie Mei, Jincan Che, Yunzhu Shi, Yudian Fang, Rongling Wu, Xuli Zhu

Subject: Biology And Life Sciences, Plant Sciences Keywords: transgenerational; functional mapping; QTL; light intensity

Online: 2 July 2024 (11:09:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0185.v1

Challenges and Opportunities in the Sustainable Improvement of Carrot Production

Antonello Paparella, Prasada Rao Kongala, Annalisa Serio, Chiara Rossi, Liora Shaltiel-Harpaza, Amjad M. Husaini, Mwafaq Ibdah

Subject: Biology And Life Sciences, Plant Sciences Keywords: Abiotic-Stress; Agriculture; Biotic-Stress; Carrot; Production

Online: 2 July 2024 (05:48:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202407.0087.v1

Revealing the Impact of Understory Fires on Stem Survival in Five Palm Species (Arecaceae): An Experimental Approach Using Predictive Models

Marcus Vinicius de Athaydes Liesenfeld

Subject: Biology And Life Sciences, Plant Sciences Keywords: Amazon Rainforest; surface fires; Acre; Brazil; post-fire fate.

Online: 1 July 2024 (13:10:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1954.v1

Genetic Diversity of Subtropical Soybean [Glycine max (L.) Merrill] Genotypes Based on SSR Markers

Hapson Mushoriwa, Isack Mathew, Pangirayi Tongoona, Eastonce T. Gwata, Godfree Chigeza, Ibnou Dieng, Abush A. Tesfaye, John Derera

Subject: Biology And Life Sciences, Plant Sciences Keywords: Diversity; Genotype; Soybean germplasm; SSR markers

Online: 1 July 2024 (10:55:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.2063.v1

Variation in the Floral Morphology of Prosthechea karwinskii (Orchidaceae), a Mexican Endemic Orchid at Risk

María Hipólita Santos-Escamilla, Gabriela Cruz-Lustre, Manuel Adrián Cuéllar-Martínez, Luicita Lagunez-Rivera, Rodolfo Solano

Subject: Biology And Life Sciences, Plant Sciences Keywords: endangered species; epiphyte; floral variation; intraspecific variation; morphometrics; orchids; ornamental/useful species.

Online: 29 June 2024 (00:16:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1921.v1

The Potential of Helichsryum splendidum for Restoration of Sites Polluted with Coal Fly Ash

Alexis Munyengabe, Ledwaba Samuel Kamogelo, Titus Ngmenzuma, Maria Fezile Banda

Subject: Biology And Life Sciences, Plant Sciences Keywords: Phytoremediation; Helichrysum splendidum; Translocation factor; Bioconcentration factor; Trace metals; Gas exchange analysis

Online: 27 June 2024 (11:03:44 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202406.1917.v1

Cell Wall Profiling of the Resurrection Plants Craterostigma plantagineum and Lindernia brevidens and Their Desiccation Sensitive Relative Lindernia subracemosa

John P Moore, Brock Kuhlman, Jeanett Hansen, Leonardo Gomez, Bodil JØrgensen, Dorothea Bartels

Subject: Biology And Life Sciences, Plant Sciences Keywords: Craterostigma; Lindernea; evolution; glycan microarrays; monosaccharides; cell walls

Online: 27 June 2024 (10:51:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202406.1846.v1

Microalgal Diversity and Molecular Ecology: A Comparative Study of Classical and Metagenomic Approaches in Ponds of the Eifel National Park, Germany

Karl-Heinz Linne von Berg, Leonie Agnes Keilholz, Nadine Küchenmeister, Ekaterina Pushkareva, Burkhard Becker

Subject: Biology And Life Sciences, Plant Sciences Keywords: microalgae; microbial diversity; metagenomics; freshwater

Online: 26 June 2024 (10:48:15 CEST)

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Biology and Life Sciences