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Preprint COMMUNICATION | doi:10.20944/preprints201911.0193.v1

Human Origins in Southern African Palaeo-wetlands? Strong Claims from Weak Evidence

Carina M. Schlebusch, Liisa Loog, Huw S. Groucutt, Turi King, Adam Rutherford, Chiara Barbieri, Guido Barbujani, Lounès Chikhi, Mattias Jakobsson, Anders Eriksson, Andrea Manica, Sarah A. Tishkoff, Eleanor M.L. Scerri, Aylwyn Scally, Chris Brierley, Mark G. Thomas

Subject: Biology And Life Sciences, Life Sciences Keywords: human origins; mitochondrial DNA; Africa; human evolution

Online: 17 November 2019 (00:55:26 CET)

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Preprint REVIEW | doi:10.20944/preprints201712.0170.v1

Life Emerged from [GADV]-Protein World, but Not from RNA World!?

Kenji Ikehara

Subject: Biology And Life Sciences, Life Sciences Keywords: RNA world; [GADV]-protein world; GADV hypothesis; origin of life; protein 0th-order structure; origin of protein; origin of genetic code; origin of gene

Online: 25 December 2017 (08:08:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints201806.0035.v2

The First Universal Common Ancestor (FUCA) as the Earliest Ancestor of LUCA’s (Last UCA) Lineage

Francisco Prosdocimi, Marco José, Sávio Farias

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; LUCA; FUCA; RNA World; PTC; Archaea; translation system

Online: 23 July 2019 (08:13:32 CEST)

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Preprint REVIEW | doi:10.20944/preprints202311.1554.v1

Mitochondrial Dysfunction and Coenzyme Q10 Supplementation in Post-Viral Fatigue Syndrome: An Overview

David Mantle, Iain Parry Hargreaves, Joan Carles Domingo, Jesus Castro-Marrero

Subject: Biology And Life Sciences, Life Sciences Keywords: chronic fatigue syndrome; coenzyme Q10; fibromyalgia; Long COVID; mitochondrial dysfunction; myalgic encephalomyelitis; post-viral fatigue syndrome

Online: 24 November 2023 (11:27:34 CET)

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Preprint REVIEW | doi:10.20944/preprints202202.0031.v1

Origin and Early Evolution of Photosynthesis: A Brief Historical Account

Tanai Cardona

Subject: Biology And Life Sciences, Life Sciences Keywords: photosynthesis; photosystem; water oxidation; oxygenic; evolution; origin of life

Online: 2 February 2022 (12:17:37 CET)

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Working Paper ARTICLE

Mechanical Energy before Chemical Energy at the Origins of Life?

H. Greenwood Hansma

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; origins of life; mechanical energy; work; entropic forces; mica; biotite; Muscovite; wet/dry cycles; clay

Online: 29 April 2019 (07:51:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201910.0086.v1

How Codon Usage Bias Affects Our Ability to Recover the Tree of Life

Justin B. Miller, Michael F. Whiting, John S.K. Kauwe, Perry G. Ridge

Subject: Biology And Life Sciences, Life Sciences Keywords: phylogenomics; phylogenetics; codon usage bias; Tree of Life

Online: 8 October 2019 (10:43:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202009.0409.v1

Did Biology Emerge from Biotite in Micaceous Clay?

H. Greenwood Hansma

Subject: Biology And Life Sciences, Life Sciences Keywords: clay; mica; biotite; muscovite; origin of life; origins of life; mechanical energy; work; wet-dry cycles

Online: 17 September 2020 (13:01:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.0223.v1

Phytochemical Contain, Biological Properties and Toxicities of Herbals Used for Doping

Modeste Traore, Samson Guenne, Ghislain Tewende Kobende, Arthur Arnaud Armand Dabone, Mamounata Diao, Hemayoro Sama, Juste Y. Bonzi, Gérard Coulibaly, Adama Hilou, Martin Kiendrebeogo

Subject: Biology And Life Sciences, Life Sciences Keywords: doping herbal; phytomolecule; antioxidant; antifatigue; phytotoxicity physical exercise

Online: 3 January 2024 (09:43:27 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202309.0079.v1

Data Literacy in Genome Research

Katharina Wolff, Ronja Friedhoff, Friderieke Schwarzer, Boas Pucker

Subject: Biology And Life Sciences, Life Sciences Keywords: computational biology; genomics; sequencing; data literacy; bioinformatics; education

Online: 4 September 2023 (04:32:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1334.v1

Resistance Exercise and Creatine Supplementation on Fat Mass in Adults ≪ 50 Years of Age: A Systematic Review and Meta-Analysis

Konstantinos Prokopidis, Scott Forbes, Darren G. Candow, Flavia Rusterholz, Bill Campbell, Sergej Ostojic

Subject: Biology And Life Sciences, Life Sciences Keywords: body composition; ergogenic aids; adipose tissue; strength training

Online: 19 June 2023 (09:06:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0223.v1

The Luminous Fungi of Japan

Yuichi Oba, Kentaro Hosaka

Subject: Biology And Life Sciences, Life Sciences Keywords: bioluminescence; fungi; mushroom; Japan

Online: 4 May 2023 (08:01:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0231.v1

The Life Sequence Conjecture and the Theory of the Reptoid

Ozzy K. King

Subject: Biology And Life Sciences, Life Sciences Keywords: reptilian; reptoid; ufology; Life Sequence Conjecture; extraterrestrials; convergent evolution; homologous evolution; parallel evolution; divergent evolution; chaos evolution

Online: 12 April 2023 (03:09:29 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.1723.v1

Biological Importance of Arginine: Roles in Structure, Disorder, and Functionality of Peptides and Proteins

Munishwar Nath Gupta, Vladimir N. Uversky

Subject: Biology And Life Sciences, Life Sciences Keywords: molecular tweezers; cation-pi interactions; intrinsic disorder, protein-protein interactions; hydrophobic interaction chromatography, monoclonal antibody production; protein aggregation; protein refolding; arginine methyltransferases; post-translational modification

Online: 27 October 2023 (05:51:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202212.0272.v2

Apiaceae Medicinal Plants: A Review of Traditional Uses, Phytochemistry, Bolting and Flowering, and Controlling Approaches

Meiling Li, Min Li, Li Wang, Mengfei Li, Jianhe Wei

Subject: Biology And Life Sciences, Life Sciences Keywords: Apiaceae plants; traditional use; phytochemistry; bolting and flowering; controlling approaches

Online: 5 May 2023 (05:47:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.1202.v2

Comparison of the Efficiency of Ultrafiltration, Precipitation, and Ultracentrifugation Methods for Exosome Isolation

Farshid Jaberi Ansari, Hossein Ahmadi Tafti, Amir Amanzadeh, Shahram Rabbani, Mohammad Ali Shokrgozar, Reza Heidari, Javad Behroozi, Vladimir N. Uversky, Hossein Ghanbari

Subject: Biology And Life Sciences, Life Sciences Keywords: exosomes; extracellular vesicles; isolation methods; ultrafiltration; precipitation; ultracentrifugation

Online: 18 August 2023 (08:19:24 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0160.v1

Biophysical Interactions Underpin the Emergence of Information in the Genetic Code

Aaron Halpern, Lilly R Bartsch, Kaan Ibrahim, Stuart A Harrison, Minkoo Ahn, John Christodoulou, Nick Lane

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; genetic code; biophysical interactions; hydrophobicity; anticodon; molecular dynamics; NMR

Online: 10 April 2023 (09:07:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0433.v1

Super-Resolution Ultrasound Localization Microscopy using High-Frequency Ultrasound to Measure Ocular Perfusion Velocity in the Rat Eye

Hasan Ul Banna, Benjamin Mitchell, Stephen Chen, Joel Palko

Subject: Biology And Life Sciences, Life Sciences Keywords: glaucoma; intraocular pressure; ocular hemodynamics; ocular vasculature; super- 19 resolution ultrasound; ultrasound localization microscopy

Online: 17 April 2023 (09:40:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1601.v1

Antimicrobial Potential of the Microalgae Extracts

Alexander Lykov, Alexei Salmin, Ruslan Gevorgiz, Svetlana Zheleznova, Lyubov Rachkovskaya, Maria Surovtseva, Olga Poveshchenko

Subject: Biology And Life Sciences, Life Sciences Keywords: diatoms; cyanobacter; green microalgae; Gram-positive and Gram-negative bacteria; Mycobacteria; antimicrobial potential

Online: 24 July 2023 (10:46:37 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0418.v1

CTHRC1: An Emerging Biomarker in Lung Fibrosis

Zhussipbek Mukhatayev, Altynay Adilbayeva, Jeannette Kunz

Subject: Biology And Life Sciences, Life Sciences Keywords: Biomarker; CTHRC1; extracellular matrix; interstitial lung disease; idiopathic pulmonary fibrosis; rheumatoid arthritis; post-COVID; pirfenidone

Online: 6 September 2023 (10:20:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1881.v1

Antiproliferative Activity of Euphorbia ingens Extract against Prostate Cancer Cell Line: An in silico and in vitro Analysis

Innocent Oluwaseun Okpako, Florence Atieno Ng'ong'a, Cleophas Mutinda Kyama, John Chukuwma Oyem, Sospeter Ngoci Njeru

Subject: Biology And Life Sciences, Life Sciences Keywords: Euphorbia ingens; Anticancer; Antiproliferative; Prostate Cancer; Network Pharmacology; Phyto-chemicals; Mechanism of action; Molecular targets

Online: 26 May 2023 (07:46:44 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0414.v1

Potential Benefits of Omega-3 Polyunsaturated Fatty Acids (N3PUFAs) on Cardiovascular Health Associated with COVID-19: An Update for 2023

Louise Weiwei Lu, Siew-Young Quek, Shiping Lu, Jie-Hua Chen

Subject: Biology And Life Sciences, Life Sciences Keywords: N3PUFA; EPA; DHA; omega-3; cardiovascular; coagulation; thrombosis; COVID-19; inflammation; oxidative stress.

Online: 17 April 2023 (07:01:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0478.v2

Mutant IDH in Gliomas: Role in Cancer and Treatment Options

Georgios Solomou, Alina Finch, Asim Asghar, Chiara Bardella

Subject: Biology And Life Sciences, Life Sciences Keywords: isocitrate dehydrogenase; cancer metabolism; hydroxyglutarate; gliomas; oncometabolites

Online: 20 April 2023 (02:28:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1988.v1

Recent Advances in Seaweed Biorefineries and Assessment of Their Potential for Carbon Capture and Storage

Katherine G. Johnston, Abdelfatah Abomohra, Christopher E. French, Abdelrahman S. Zaky

Subject: Biology And Life Sciences, Life Sciences Keywords: Macroalgae; Microalgae; Yeast; Biomass conversion; Climate change; Circular bioeconomy

Online: 28 July 2023 (13:36:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.0667.v1

Drosophila melanogaster as a Translational Model System to Explore the Impact of Phytochemicals on Human Health

Carlos Lopez-Ortiz, Celeste Gracia-Rodriguez, Samantha Belcher, Gerardo Flores-Iga, Amartya Das, Padma Nimmakayala, Nagamani Balagurusamy, Umesh K Reddy

Subject: Biology And Life Sciences, Life Sciences Keywords: Drosophila; Phytochemical; Human health; Metabolism; Disease

Online: 9 August 2023 (05:12:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1595.v1

Non-coding RNAs as Key Regulators of the Notch Signaling Pathway in Glioblastoma: Diagnostic, Prognostic, and Therapeutic Targets

Seyed Hossein Shahcheraghi, Elmira Roshani Asl, Malihe Lotfi, Jamshid Ayatollahi, Seyed Hossein Khaleghinejad, Alaa A.A. Aljabali, Hamid Bakshi, Mohamed El-Tanani, Najwane Said-Sadier, Abdulmajeed G. Almutary, Ángel Serrano-Aroca, Vijay Mishra, Yachana Mishra, Rohit Goyal, Altijana Hromić-Jahjefendić, Vladimir N. Uversky, Marzieh Lotfi, Murtaza M. Tambuwala

Subject: Biology And Life Sciences, Life Sciences Keywords: Glioblastoma, Notch signaling, lncRNAs, miRNAs

Online: 24 July 2023 (10:51:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1153.v1

Characterization of a C57BL/6J Fancg-KO Mouse Model Generated by CRISPR/Cas9

Ronak Shah, Paul C.M. Van den Berk, Colin E.J. Pritchard, Ji-Ying Song, Maaike Kreft, Bas Pilzecker, Heinz Jacobs

Subject: Biology And Life Sciences, Life Sciences Keywords: Fanconi anemia (FA); FANCG, interstrand crosslink (ICL); DNA damage response; genetically engineered mouse model (GEMM); CRISPR/Cas9; cisplatin; Mitomycin C (MMC); hematopoiesis

Online: 15 June 2023 (13:29:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1661.v1

Gravisensitivity Of Stromal Collagen Fibers In The Gastrointestinal Hollow Organs Of Mice After A 30-Day Space Flight

Viktoriya Shishkina, Andrey Kostin, Nataliya T Alexeeva, Svetlana V Klochkova, Dmitry B Nikityuk, Artem Volodkin, Igor Buchwalow, Markus Tiemann, Dmitri Atiakshin

Subject: Biology And Life Sciences, Life Sciences Keywords: orbital flight; weightlessness; digestive system; connective tissue; collagen fibers; С57BL/6N mice

Online: 25 July 2023 (08:10:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0357.v1

Polyphenols Analysis by LC-MS-ESI and Potent Antioxidant, Anti-Inflammatory, Antimicrobial Activities of Jatropha multifida L. Extracts Used in Benin Pharmacopoeia

Durand Dah-Nouvlessounon, Michaelle Chokki, Essé A Agossou, Jean-Baptiste Houédanou, Martial Nounagnon, Haziz Sina, Romana Vulturar, Simona Codruta Heghes, Angela Cozma, Jacques François Mavoungou, Adriana Fodor, Farid Baba-Moussa, Ramona Suharoschi, Lamine Baba-Moussa

Subject: Biology And Life Sciences, Life Sciences Keywords: Plant extract; LC-MS-ESI; Bioactif compound; Biological activities; Benin

Online: 4 August 2023 (07:41:44 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202304.0815.v1

Countering Biothreats from Low-Effort Toxins: Some Highlights from the Chemical and Biological Investigation, Evidence, and Countermeasures Training

Subhash Janardhan Bhore

Subject: Biology And Life Sciences, Life Sciences Keywords: biosafety; biorisk; biosecurity; biosecurity engagement program; bioterrorism; CBRNE; cooperative threat reduction; homemade explosives; southeast Asia; sustainability; toxins

Online: 24 April 2023 (03:57:39 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.0196.v1

“When”, “Where”, and “How” of SARS-CoV-2 Infection Affects the Human Cardiovascular System: a Narrative Review

Nicholas G. Kounis, Christos Gogos, Cesare de Gregorio, Ming-Yow Hung, Sophia N. Kouni, Efthymios P. Tsounis, Stelios F. Assimakopoulos, Soheila Pourmasumi, Virginia Mplani, George Servos, Periklis Dousdampanis, Panagiotis Plotas, Marina A. Michalaki, Grigorios Tsigkas, Gerasimos Grammatikopoulos, Dimitrios Velissaris, Ioanna Koniari

Subject: Biology And Life Sciences, Life Sciences Keywords: ACE2 receptors; COVID-19; cardiac complications, kinin-kallikrein; Kounis syndrome

Online: 2 August 2023 (10:07:00 CEST)

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The novel severe acute respiratory coronavirus-2 (SARS-Cov-2) constitutes the causative agent of coronavirus disease-2019 (COVID-19). Several mechanisms have been proposed regarding the development of cardiovascular complications during and after acute COVID-19 infection. COVID-19, caused by SARS-CoV-2, has emerged as one of the deadliest pandemics in modern history. Several clinical and pathophysiologic mechanisms have been proposed for development of cardiovascular complications during and after acute COVID-19 infection. The myocardial injury in patients with COVID-19 has been attributed to coronary spasm, microthrombi formation, plaque rupture, hypoxic injury or cytokine storm disposing the same pathophysiology with the three clinical variants of Kounis syndrome. One of the main proposed mechanisms for development of cardiovascular complications is the angiotensin converting enzyme 2 (ACE2) and its interactions with the renin-aldosterone system (RAAS) and the kinin-kallikrein system (KKS). The ACE2 receptors are expressed throughout the human body located mainly in heart, blood vessels endothelium, lungs, intestines, testes and neurons. The SARS-CoV-2 directly invades the endothelial cells that contain ACE2 receptors and constitutes the main pathway through which the virus enters the endothelial cells. This leads to downregulation of the ACE2 receptors and causes angiotensin II accumulation leading to prothrombotic effects such as hemostatic imbalance via activation of the coagulation cascade, impaired fibrinolysis, thrombin generation, vasoconstriction, endothelial and platelet activation, and pro-inflammatory cytokine release. Indeed, angiotensin I exerts no direct biological function except that its high levels can stimulate catecholamine production. It is metabolized to its biologically active byproduct angiotensin II, a potent vasoconstrictor, by angiotensin converting enzyme (ACE) through cleavage of the two terminal amino acids. SARS-CoV-2 infection prevents the counterbalancing action of the KKS system that normally causes vasodilation and regulates tissue repair, inflammation, cell proliferation, and platelet aggregation. All this cascade leads to the following cardiovascular events: cardiac arrhythmias, cardiac arrest, cardiomyopathy, cytokine storm, heart failure, ischemic myocardial injuries and microvascular disease, Kounis syndrome, long COVID, myocardial fibrosis, myocarditis, new onset hypertension, pericarditis, postural orthostatic tachycardia syndrome (POTS), pulmonary hypertension, stroke, Takotsubo Syndrome, venous thromboembolism and thrombocytopenia. In this narrative review we describe and elucidate when and how the COVID-19 affects the human cardiovascular system, in various areas on the human body that are vulnerable in every category of patients including children and athletes.

Preprint REVIEW | doi:10.20944/preprints202304.0478.v1

Mutant IDH in Gliomas: Role in Cancer and Treatment Options

Georgios Solomou, Alina Finch, Asim Asghar, Chiara Bardella

Subject: Biology And Life Sciences, Life Sciences Keywords: isocitrate dehydrogenase, cancer metabolism, hydroxyglutarate, gliomas, oncometabolites

Online: 18 April 2023 (04:12:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.2038.v1

Harnessing Mechanical Forces for Cancer Treatment

Alka Kumari, S. Manasa Veena, Rashmita Luha, Ajay Tijore

Subject: Biology And Life Sciences, Life Sciences Keywords: mechanical force; apoptosis; tumor; tumor microenvironment; mechanomedicine

Online: 31 July 2023 (11:36:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202309.0846.v1

Rhabdomyosarcoma: Current Therapy, Challenges, and Future Approaches to Treatment Strategies

Ali Zarrabi, David Perrin, Mahboubeh Kavoosi, Micah Sommer, Serap Sezen, Parvaneh Mehrbod, Bhavya Bhushan, Filip Machaj, Jakub Rosik, Philip Kawalec, Saba Afifi, Seyed Mohammadreza Bolandi, Peyman Koleini, Mohsen Taheri, Tayyebeh Madrakian, Marek Jan Łos, Benjamin Lindsey, Nilufer Cakir, Atefeh Zarepour, Kiavash Hushmandi, Ali Fallah, Bahattin Koc, Mazaher Ahmadi, Susan E. Logue, Gorka Orive, Stevan Pecic, Joseph W. Gordon, Saeid Ghavami

Subject: Biology And Life Sciences, Life Sciences Keywords: Alveolar Rhabdomyosarcoma; Apoptosis; Autophagy; Unfolded Protein Response; Bioengineering; Tumor Stiffness

Online: 13 September 2023 (10:32:09 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202304.0041.v1

Identification of Novel Biomarkers in Peripheral Blood Mononuclear Cells of Hepatocellular Carcinoma Patients

Sindhoora R

Subject: Biology And Life Sciences, Life Sciences Keywords: Hepatology; Oncology; Biomarkers; Hepatocellular Carcinoma

Online: 4 April 2023 (09:28:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1144.v1

Filtering Methods for Biomedical Image Denoising

Ting Zhao

Subject: Biology And Life Sciences, Life Sciences Keywords: image denoising; filtering methods; biomedical image denoising; healthcare; adaptive filtering methods

Online: 18 October 2023 (09:18:36 CEST)

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In this paper, the filtering method of biomedical image denoising is described comprehensively. Firstly, it introduces the biomedical image denoising, describes the relationship between biomedical image denoising and medical care, introduces the filtering methods, the filtering methods of biomedical image denoising, the challenges encountered by the current filtering methods, and other application fields of filtering methods. Firstly, the background of biomedical image denoising is introduced. Biomedical image denoising is a challenge. Different imaging modes have different noise characteristics, and noise levels can vary greatly depending on the specific application. Secondly, it describes that biomedical image denoising plays an important role in medical care, and the biomedical image directly affects the patient's diagnosis, treatment plan and the overall quality of medical care service. Then the filtering method is introduced in detail, describing the core concepts and related features of linear filtering, nonlinear filtering and frequency domain filtering, and then focusing on the adaptive filtering method, describing the characteristics, conditions of use, common algorithms and advantages of adaptive filtering method. Then the filter methods of biomedical image denoising are introduced, and the core concepts of Gaussian filter, median filter, total variation denoising and Wiener filter are introduced respectively. Then, the challenges encountered by filtering methods are described, such as the accurate selection of filters, the balance between noise reduction and image detail preservation are introduced. Finally, the application of filtering method in other fields is mentioned, such as audio processing, speech recognition and so on. In summary, this paper comprehensively expounds the denoising and filtering methods of biomedical images, the filtering methods of medical image denoising, the relationship between medical image denoising and medical care, and the challenges encountered by filtering methods.

Preprint REVIEW | doi:10.20944/preprints202401.0635.v1

A Comprehensive Review of Wolbachia-Mediated Mechanisms to Control Dengue Virus Transmission in Aedes aegypti through Innate Immune Pathways

Muhammad Sajjad Sarwar, Iqra Mushtaq, Iqra Munzoor

Subject: Biology And Life Sciences, Life Sciences Keywords: Wolbachia, Ae. aegypti, DENV, arboviruses, Innate immune pathways, mechanisms.

Online: 8 January 2024 (12:55:37 CET)

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Preprint REVIEW | doi:10.20944/preprints202306.1223.v1

Prostate Cancer: Genetics, Epigenetics and the Need for Immunological Biomarkers

Guzel Rafikova, Irina Gilyazova, Kadriia Enikeeva, Valentin Pavlov, Julia G. Kzhyshkowska

Subject: Biology And Life Sciences, Life Sciences Keywords: cancer genetics; cancer epigenetic; tumor microenvironment; monocytes; TAMs; prostate cancer; cancer biomarkers

Online: 16 June 2023 (10:42:15 CEST)

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Epidemiological data highlight prostate cancer as a significant global health issue, with high incidence and substantial impact on patients' quality of life. The prevalence of this disease is associated with various factors, including age, heredity, and race. Recent research in prostate cancer genetics has identified several genetic variants that may be associated with an increased risk of developing the disease. However, despite the significance of these findings, genetic markers for prostate cancer are not currently utilized in clinical practice as reliable indicators of the disease. In addition to genetics, epigenetic alterations also play a crucial role in prostate cancer development. Aberrant DNA methylation, changes in chromatin structure, and microRNA (miRNA) expression are major epigenetic events that influence oncogenesis. Existing markers for prostate cancer, such as prostate-specific antigen (PSA), have limitations in terms of sensitivity and specificity. The cost of testing, follow-up procedures, and treatment for false-positive results and overdiagnosis contributes to the overall healthcare expenditure. Improving the effectiveness of prostate cancer diagnosis and prognosis requires either narrowing the risk group by identifying new genetic factors or enhancing the sensitivity and specificity of existing markers. Immunological biomarkers (both circulating and intra-tumoral), including markers of immune response and immune dysfunction, represent a potentially useful area of research for enhancing the diagnosis and prognosis of prostate cancer. Our review emphasizes the need for developing novel immunological biomarkers to improve the diagnosis, prognosis, and management of prostate cancer. We highlight the most recent achievements in the identification of biomarkers provided by circulating monocytes and tumor-associated macrophages (TAMs). We highlight that monocytes-derived and TAMs-derived biomarkers can enable to establish the missing links between genetic predisposition, hormonal metabolism and immune responses in prostate cancer.

Preprint ARTICLE | doi:10.20944/preprints202311.0853.v1

Stress-Granules, P-bodies, and Cell Aging: A Bioinformatics Study

Yakov I. Mokin, Nikolay S. Ilyinsky, Semen V. Nesterov, Eugene Y. Smirnov, Olga S. Sergeeva, Anna E. Romanovich, Irina M. Kuznetsova, Konstantin K. Turoverov, Vladimir N. Uversky, Alexander V. Fonin

Subject: Biology And Life Sciences, Life Sciences Keywords: liquid-liquid phase separation; membrane-less organelles; stress granules; P-bodies; intrinsically disordered proteins; intrinsically disordered regions; aging; senescence; protein aggregation; nucleic acids; RNA-binding protein; DNA-binding protein

Online: 14 November 2023 (16:38:59 CET)

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At the molecular level, aging is often accompanied by dysfunction of stress-induced membrane-less organelles (MLOs) and changes in their material state. In this work, we analyzed the proteins included in the proteome of stress granules (SGs) and P-bodies for their tendency to transform the material state of these MLOs. Particular attention was paid to proteins whose gene expression changes during replicative aging. It was shown that the proteome of the studied MLOs practically does not differ in the analyzed characteristics and consists of completely or partially intrinsically disordered proteins, 30 - 40% of which are potentially capable of liquid-liquid phase separation (LLPS). At the same time, the proportion of proteins capable of spontaneous LLPS is relatively small, which indicates the leading role of nucleic acids in the biogenesis of these membrane-less organelles. Proteins whose gene expression changes during the transition of human cells to a senescent state make up about 20% of the studied proteomes. There is a statistically significant increase in the number of positively charged proteins in both datasets studied compared to the complete proteomes of these organelles. An increase in the relative content of DNA-, but not RNA-binding proteins, was also found in the stress-granules dataset with senescence-related processes. Among SG proteins potentially involved in senescent processes, there is an increase in the abundance of potentially amyloidogenic proteins compared to the whole proteome. The hnRPDL protein has the highest degree of disorder and highest propensity for LLPS among such proteins, which allows us to consider it as “potentially dangerous.” Proteins common to SGs and P bodies, potentially involved in processes associated with senescence, form clusters of interacting proteins. The largest cluster is represented by RNA-binding proteins involved in RNA processing and translation regulation. These data indicate that SG proteins, but not proteins of P-bodies, are more likely to transform the material state of MLOs. Furthermore, these MLOs can participate in processes associated with aging in a coordinated manner.

Preprint ARTICLE | doi:10.20944/preprints202306.1198.v1

L-Citrulline Supplementation Does Not Affect Blood Pressure and Arterial Stiffness at Rest and During Isometric Exercise in Older Adults

Andrea Tryfonos, Filippos Christodoulou, George M. Pamboris, Stephanos Christodoulides, Anastasios A. Theodorou

Subject: Biology And Life Sciences, Life Sciences Keywords: nitric oxide; dietary supplements; endothelium function; hypertension; cardiovascular disease; blood flow

Online: 16 June 2023 (08:39:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202303.0448.v1

On the Roles of the Nuclear Non-coding RNA-Dependent Membrane-Less Organelles in the Cellular Stress Response

Anastasia A. Gavrilova, Anna S. Fefilova, Irina M. Kuznetsova, Konstantin K. Turoverov, Vladimir N. Uversky, Alexander V. Fonin

Subject: Biology And Life Sciences, Life Sciences Keywords: LLPS; liquid-liquid phase separation; non-coding RNA; intrinsically disordered proteins; membrane-less organelles; stress-response; biomolecular condensates

Online: 27 March 2023 (05:43:50 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1004.v1

Cuban Policosanol (Raydel®) Exhibited Potent Anti-inflammatory Activity in the Liver and Protection of the Ovary and Testis with an Improvement of Blood Lipid Profile by 12 Weeks Supplementation in Hyperlipidemic Zebrafish: Comparison Study of Efficacy with Three Chinese Policosanols

Kyung-Hyun Cho, Ji-Eun Kim, Seung Hee Baek

Subject: Biology And Life Sciences, Life Sciences Keywords: Policosanol; high cholesterol diet; high-density lipoproteins; apoA-I; inflammation; interleukin-6; liver; testis; ovary

Online: 14 July 2023 (09:27:04 CEST)

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Although many policosanols from different sources, such as sugar cane, rice bran, and insects, have been marketed worldwide, there has been no comparative study of the in vivo efficacy using a hyperlipidemic animal model. This study compared the efficacy of treating dyslipidemia with four different policosanols, including one sugar cane wax alcohol from Cuba (Raydel®) and three policosanols from China (Xi’an Natural sugar cane, Xi’an Realin sugar cane, and Shaanxi rice bran) in hyperlipidemic zebrafish. After 12 weeks of consumption of each policosanol (final 0.1% in diet, wt/wt) and high cholesterol diet (HCD, final 4%, wt/wt) in a tetrabit, the Raydel policosanol group and Xi’an Natural policosanol group showed the highest survivability of approximately 81%, whereas the Xi’an Realin policosanol group and Shaanxi policosanol group showed 57% and 67% survivability, respectively. Among five HCD groups, the Raydel policosanol group showed the lowest serum total cholesterol (TC, p<0.001 versus HCD control) and triglyceride (TG, p<0.001 versus HCD control) with the highest percentage of high-density lipoproteins (HDL)-C in TC. The Raydel policosanol group also showed the lowest serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels with the least infiltration of inflammatory cells and interleukin-6 production in hepatocytes. In the ovary, the Raydel policosanol group also showed the highest contents of mature vitellogenic oocytes with the lowest production of reactive oxygen species and cellular apoptosis in ovarian cells. In the testis, the Raydel policosanol group also showed the healthiest morphology for spermatogenesis with the least interstitial area and reactive oxygen species (ROS) production in testicular cells. In conclusion, 12 weeks of Raydel policosanol consumption resulted in remarkable improvement of the blood lipid profile, amelioration of inflammation in the liver, and enhancement of the cell morphology in the ovary and testis with the highest survivability under the HCD.

Preprint REVIEW | doi:10.20944/preprints202401.1692.v1

Effectiveness of “Escape Room” Educational Technology in Nurses’ Education: A Systematic Review.

Héctor González-de la Torre, María-Naira Henández-De Luis, Sergio Mies-Padilla, Rafaela Camacho-Bejarano, José Verdú-Soriano, Claudio-Alberto Rodríguez-Suárez

Subject: Biology And Life Sciences, Life Sciences Keywords: Education, Nursing; Educational Technology; Gamification; Systematic Review

Online: 23 January 2024 (12:13:39 CET)

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Preprint REVIEW | doi:10.20944/preprints202305.1357.v1

TRP Channels in Tumoral Processes Mediated by Oxidative Stress and Inflammation: Friends or Foes?

Florentina Piciu, Mihaela Balas, Madalina Andreea Badea, Dana Cucu

Subject: Biology And Life Sciences, Life Sciences Keywords: ROS; TRP; inflammation

Online: 19 May 2023 (02:09:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1293.v1

Recent Developments in High Spatio-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications

Wenfa Ng

Subject: Biology And Life Sciences, Life Sciences Keywords: spatial proteomics; spatial resolution; temporal resolution; super-resolution microscopy; fluorescence microscopy,

Online: 18 May 2023 (07:54:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0125.v1

De Novo Venom Gland Transcriptome Assembly and Characterization for Calloselasma rhodostoma (Kuhl, 1824), the Malayan Pit Viper from Malaysia: Unravelling Toxin Gene Diversity in a Medically Important Basal Crotaline

Choo Hock Tan, Kae Yi Tan, Tzu Shan Ng, Nget Hong Tan, Ho Phin Chong

Subject: Biology And Life Sciences, Life Sciences Keywords: kistomin; rhodostoxin; rhodostomin; rhodocytin; rhodocetin; ancrod

Online: 7 April 2023 (11:36:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0515.v1

Cryopreservation of Ovarian and Testicular Tissue and the Influence on Epigenetic Pattern

Tom Trapphoff, Stefan Dieterle

Subject: Biology And Life Sciences, Life Sciences Keywords: epigenetic; genomic imprinting; ovarian tissue cryopreservation; testicular tissue cryopreservation; Medically Assisted Reproduction

Online: 7 June 2023 (08:32:11 CEST)

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Ovarian tissue cryopreservation (OTC) or testicular tissue cryopreservation (TTC) are effective and often the only options for fertility preservation in female or male patients due to oncological, medical, or social aspects. While TTC and resumption of spermatogenesis, either in vivo or in vitro, has still be considered an experimental approach in humans, OTC and autotransplantation has been applied increasingly to preserve fertility with more than 200 live births worldwide. However, the cryopreservation of reproductive cells followed by the resumption of gametogenesis, either in vivo or in vitro, may interfere with sensitive and highly regulated cellular processes. In particular, the epigenetic profile, which includes not just reversible modifications of the DNA itself but also post-translational histone modifications, small non-coding RNAs, gene expression and availability, and storage of related proteins or transcripts, have to be considered in this context. Due to complex reprogramming and maintenance mechanisms of the epigenome in germ cells, growing embryos, and offspring, OTC and TTC are carried out at very critical moments early in the life cycle. Given this background, the safety of OTC and TTC taking into account the epigenetic profile has to be clarified. Cryopreservation of mature germ cells (including Metaphase II oocytes and mature spermatozoa collected via ejaculation or more invasively after testicular biopsy) or embryos has been used successfully for many years in Medically Assisted Reproduction (MAR). However, tissue freezing followed by in vitro or in vivo gametogenesis has become more attractive in the past, while few human studies have analysed the epigenetic effects, with most data deriving from animal studies. In this review, we highlight the potential influence of the cryopreservation of immature germ cells and subsequent in vivo or in vitro growth and differentiation on the epigenetic profile in humans and animals.

Preprint REVIEW | doi:10.20944/preprints202312.0536.v1

DNA Barcoding as a Plant Identification Method

Stavroula Letsiou, Panagiotis Madesis, Efstathios Vasdekis, Cinzia Montemurro, Maria E. Grigoriou, George Skavdis, Vasillios Moussis, Antonios E. Koutelidakis, Andreas G. Tzakos

Subject: Biology And Life Sciences, Life Sciences Keywords: DNA Barcoding; species identification; taxonomy; plants; molecular markers

Online: 8 December 2023 (03:34:41 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1861.v1

Current Advances in Bispecific T Cell–Engaging Therapies

Emmanuel Owusu Ansah, Bernard Ansong Nyarko

Subject: Biology And Life Sciences, Life Sciences Keywords: Bispecific; immunotherapy; cancer; cytokine; antibodies

Online: 25 January 2024 (15:51:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.1073.v1

Comparative Analysis of Cell Mixtures Deconvolution and Gene Signatures Generated for Blood, Immune and Cancer Cells

Natalia Alonso-Moreda, Alberto Berral-Gonzalez, Enrique De La Rosa, Oscar González-Velasco, Jose Manuel Sanchez-Santos, Javier De Las Rivas

Subject: Biology And Life Sciences, Life Sciences Keywords: cell mixture; deconvolution; immune cells; blood cells; cancer cells; gene signature; bioinformatics

Online: 27 April 2023 (10:34:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202308.1567.v1

Mechanisms of PIEZO Channel Inactivation

Zijing Zhou, Boris Martinac

Subject: Biology And Life Sciences, Life Sciences Keywords: mechanosensitive channels; mechanopathologies; force-from-lipids; TMEM150C; STOML3; MDFIC; MDFI

Online: 23 August 2023 (07:52:43 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1326.v1

The Effect of COVID-19 on Gut Microbiota: Exploring the Complex Interplay and Implications for Human Health

Shamima Akter, Sa’dia Tasnim, Rashu Barua, Mayank Choubey, Shahida Arbee, Mohammad Mohabbulla Mohib, Naofel Minhaz, Ajanta Choudhury, Pallab Sarker, Mohammad Sarif Mohiuddin

Subject: Biology And Life Sciences, Life Sciences Keywords: COVID-19; Gut Microbiota; Microbiome; Dysbiosis

Online: 19 July 2023 (10:06:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1265.v1

Fungi in a One Health Perspective

Daniela Simões, Eugénia De Andrade, Raquel Sabino

Subject: Biology And Life Sciences, Life Sciences Keywords: Fungal infections; Plant health; Human health; Animal health; Mycotoxins,; Antifungal resistance; Climate change

Online: 19 June 2023 (03:01:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0287.v1

The Dissipative Photochemical Origin of Life: UVC Abiogenisis of the Purines

Claudeth Clarissa Hernández, Karo Michaelian

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; disspative structuring; non-equilibrium thermodynamics; prebiotic chemistry; abiogenisis; adenine; guanine; hypoxanthine; xanthine; purines

Online: 21 June 2022 (05:14:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0739.v1

Exploring the Development of Astrobiology Scientific Research Through Bibliometric Network Analysis: A Focus on Biomining and Bioleaching

Luca Tonietti, Bernardo Barosa, Emiliano Pioltelli, Donato Giovannelli, Giovanni Covone, Paola Di Donato, Angelina Cordone, Laura Inno, Christian Magliano, Stefano Fiscale, Maria Teresa Muscari Tomajoli, Gaetana Napolitano, Alice Maria Piccirillo, Vincenzo Della Corte, Rosa Santomartino, Alessandra Rotundi

Subject: Biology And Life Sciences, Life Sciences Keywords: biomining; bioleaching; astrobiology; A. ferrooxidans; acidophiles; space biology; bibliometric network analysis

Online: 10 May 2023 (10:38:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0607.v1

From Transcription Factors to Personalized Vaccines: A Multifaceted Review of Glioblastoma Research and Treatment

Amol Tandon, Deepthi Nagalla

Subject: Biology And Life Sciences, Life Sciences Keywords: Glioblastoma; Transcription factors; Neoantigens; Personalized therapy; Epigenetics

Online: 10 October 2023 (09:55:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1106.v1

Trends in Photothermal Nanostructures for Antimicrobial Applications

Violeta Dediu, Jana Ghitman, Gratiela Gradisteanu-Pircalabioru, Kiat Hwa Chan, Florina Silvia Iliescu, Ciprian Iliescu

Subject: Biology And Life Sciences, Life Sciences Keywords: photothermal-antimicrobials; antibacterial mechanisms; anti-biofilm; wound healing

Online: 16 May 2023 (05:40:58 CEST)

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Rapid developing antimicrobial resistance due to broad antibiotic utilisation in healthcare and food industries and the non-availability of novel antibiotics represents one of the most critical public health issues worldwide. The current advances in nanotechnology allow new materials to address drug-resistant bacterial infections in specific, focused and biologically safe ways. The unique physicochemical properties, biocompatibility, and wide range of adaptability of nanomaterials that exhibit photothermal capability can be employed to develop the next generation of photothermally induced controllable hyperthermia as antibacterial nanoplatforms. Here, we review the current state-of-the-art in different functional classes of photothermal antibacterial nanomaterials and the strategies to optimise antimicrobial efficiency. The recent achievements and trends in developing photothermally active nanostructures, including plasmonic metals, semiconductor, carbon-based, and organic photothermal polymers, and antibacterial mechanisms of action, including anti-multidrug resistant bacteria and biofilms removal, will be discussed. Insights into mechanisms of the photothermal effect and various factors influencing photothermal antimicrobial performance, emphasising the structure–performance relationship, are discussed. We will examine the photothermal agents’ functionalisation for specific bacteria, effects of near-infrared light irradiation spectrum, or active photothermal materials for multimodal synergistic-based therapies to minimise side effects and maintain low cost. Most relevant applications are presented, such as anti-biofilm formation, biofilm penetration or ablation, and nanomaterial-based infected wound therapy. Practical antibacterial applications employing the photothermal antimicrobial agents, alone or in synergistic combination with other nanomaterials, are considered. Existing challenges and limitations in photothermal antimicrobial therapy and future perspectives are presented from the structure, function, safety, and clinical potential points of view.

Preprint REVIEW | doi:10.20944/preprints202306.1372.v1

Transcription Factors Control the Virulence-Associated Metabolic Enzymes and Membrane Proteins in Response to Nutrient Bioavailability in Cryptococcal Infection

Olufemi Samuel Folorunso, Olihile Moses Sebolai

Subject: Biology And Life Sciences, Life Sciences Keywords: Cryptococcus; biofilm; metabolic enzymes; virulence factors; transcription factors; membrane permeases; membrane transporters; catabolite repression

Online: 19 June 2023 (14:43:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1598.v1

Senna alata: Phytochemistry, Antioxidant, Thrombolytic, Anti-inflammatory, Cytotoxicity, Antibacterial activity, and GC-MS analysis

Deepa Karki, Bipindra Pandey, Prabhat Jha, Ashish Acharya, Dharma Khanal, Bechan Raut, Sandesh Panthi

Subject: Biology And Life Sciences, Life Sciences Keywords: Senna alata; phytochemical screening; antioxidant activity; anti-inflammatory activity; thrombolytic activity; cytotoxic activity

Online: 21 December 2023 (12:49:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.1207.v1

Malaria Risk Drivers in the Brazilian Amazon: Land Use: Land Cover Interactions and Biological Diversity

William Gonzalez-Daza, Renata Muylaert, Thadeu Sobral-Souza, Victor Lemes-Landeiro

Subject: Biology And Life Sciences, Life Sciences Keywords: Malaria; Amazon biome; INLA; Land use/cover interactions; Bird and amphibian rich-ness-endemics; Landscape composition; Biological diversity; Spatio-temporal modeling

Online: 29 April 2023 (04:29:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.0611.v1

Beyond Psychotropic: Repurposing of Fluoxetine Toward Cancer Management and Its Molecular Mechanisms

Sultan F. Kadasah, Abdulaziz M. S. Alqahtani, Mohamed O. Radwan

Subject: Biology And Life Sciences, Life Sciences Keywords: Fluoxetine; Cancer; Repurposing; multidrug resistance (MDR)

Online: 12 February 2024 (04:59:07 CET)

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Preprint REVIEW | doi:10.20944/preprints202308.1824.v1

Various Biomimetics as Antifungals

Elena Efremenko, Aysel Aslanli, Nikolay Stepanov, Olga Senko, Olga Maslova

Subject: Biology And Life Sciences, Life Sciences Keywords: antifungal peptides; enzyme-like activity; growth inhibition; antifungal activity; fungal metabolites; quorum response molecules; combined antifungals

Online: 28 August 2023 (09:44:34 CEST)

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Preprint REVIEW | doi:10.20944/preprints202401.1989.v1

Cancer Treatments: Past, Present, and Future

Dmitriy Sonkin, Anish Thomas, Beverly Teicher

Subject: Biology And Life Sciences, Life Sciences Keywords: cancer; cancer treatment; cancer treatments history; cancer therapy; oncology; immunology; chemotherapy; radiation therapy; ADC; CART; BiTE; targeted therapy; cancer genetics

Online: 30 January 2024 (03:28:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.1124.v1

Application of Ligand- and Structure-Based Prediction Models for the Design of Alkylhydrazide-Based HDAC3 Inhibitors as Novel Anti-cancer Compounds

Emre f Bülbül, Dina Robaa, Ping Sun, Fereshteh Mahmoudi, Jelena Melesina, Matthes Zessin, Mike Schutkowski, Wolfgang Sippl

Subject: Biology And Life Sciences, Life Sciences Keywords: docking; binding free energy; pharmacophore; KPLS; QSAR; alkylhydrazide; histone deacetylases (HDAC)

Online: 16 May 2023 (08:26:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0229.v7

Lipofuscin and Aging: Testing the Garbage Catastrophe Theory in cellulo

Michael Renteln

Subject: Biology And Life Sciences, Life Sciences Keywords: Anti-aging; Hydra vulgaris; TFEB; telomerase; FluidFM

Online: 7 September 2023 (03:36:15 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202305.1298.v1

The Renin-Angiotensin-System in COVID-19: Can Long COVID Be Predicted?

Simone König, Richard Vollenberg, Phil-Robin Tepasse

Subject: Biology And Life Sciences, Life Sciences Keywords: Mass spectrometry; RAS; ACE; ACE2; CPN; bradykinin; hypertension; cardiovascular disease; antihypertensiva; ACE inhibitors; angiotensin receptor blockers; bradykinin; neuropeptide re-porter assay

Online: 18 May 2023 (08:13:10 CEST)

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(1) Background: Co-morbidities such as hypertension and cardiovascular disease are major risk factors for severe COVID-19. The renin-angiotensin-system (RAS) is critically involved in their pathophysiology and is counterbalanced by both angiotensin-converting enzyme 2 (ACE2), the functional receptor of SARS-CoV-2, and the kallikrein-kinin-system (KKS). Considerable research interest with respect to COVID-19 treatment is, thus, currently directed towards the components of these systems. In an earlier study, we noticed significantly reduced carboxypeptidase N (CPN, KKS member) activity and partially excessive angiotensin-converting enzyme (ACE, RAS member) activity in the sera of both hospitalized (HoP) COVID-19 patients and a sub-group of covalescent patients, while in the majority of the probands recovering from the disease these values had returned to normal. The data had been obtained using bradykinin (BK) as a reporter peptide, which is a target of both CPN and ACE, and they were supplemented by serum proteomics of the same patient cohort. We hypothesized that the data could be indicative of Long COVID, which had not been fully appreciated at the time of our study.; (2) Methods: The data were re-evaluated in the light of Long COVID. The recent literature on the RAS in COVID-19, antihypertensiva, and Long COVID was briefly reviewed.; (3) Results: While the levels of the BK serum degradation products should return to normal concentrations during convalescence, this was not true for some patients. This could be due to persisting liver problems, because CPN is synthesized there, but also to a dysregulated RAS. This was not reflected in the levels of selected RAS/KKS serum proteins like angiotensinogen (AGT), although AGT correlated with disease severity in HoP. However, standard tests in routine patient care in Long COVID often come back normal, and it may be that BK degradation is specific in some pathophysiologies. Moreover, the HoP group was sub-divided based on the serum protein profiles and COVID-19 severity.; (4) Conclusions: We point out two insights: 1) Sensitive technology such as omics methods might provide unexpected significant differences within the pre-defined patient groups of a clinical study. Those can only be explored, if the cohorts are large enough and properly matched with respect to the parameters known beforehand (e.g., age, gender, co-morbidities). 2) Results of the BK-reporter serum protease activity assay could be indicative of persisting liver problems and/or potentially of Long COVID. Clinical studies are required to test this hypothesis.

Preprint ARTICLE | doi:10.20944/preprints202308.0834.v1

Activation of Sarco/Endoplasmic Reticulum Ca2+-ATPase by Natural Polyphenolic Compounds. Mechanism of Protection against Oxidative Stress

Vladimír Heger, Benesova Barbora, Magdalena Majekova, Petronela Rezbarikova, Gabor Girst, Sara Hassan Hassan Ahmed, Tímea Gonda, Attila Hunyadi, Lubica Horakova

Subject: Biology And Life Sciences, Life Sciences Keywords: SERCA activity; insulin release; pancreatic beta-cells; polyphenolic compounds

Online: 10 August 2023 (08:21:09 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1708.v1

The Role of Cysteine Peptidases Cathepsins B, H, C, and X/Z in Neurodegeneration and Cancer

Veronika Stoka, Olga Vasiljeva, Hiroshi Nakanishi, Vito Turk

Subject: Biology And Life Sciences, Life Sciences Keywords: cysteine cathepsins; exopeptidases; neurodegeneration; cancer; neurodegenerative disorders

Online: 25 July 2023 (11:01:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202101.0500.v1

The Dissipative Photochemical Origin of Life: UVC Abiogenesis of Adenine

Karo Michaelian

Subject: Biology And Life Sciences, Life Sciences Keywords: origin of life; disspative structuring; prebiotic chemistry; abiogenisis; adenine

Online: 25 January 2021 (13:57:05 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.1028.v1

Evaluation of DNA Methylation Profiles of LINE-1, Alu and Ribosomal DNA Repeats in Human Cell Lines Exposed to Radiofrequency Radiation

Francesco Ravaioli, Maria Giulia Bacalini, Cristina Giuliani, Camilla Pellegrini, Chiara D'Silva, Sara De Fanti, Chiara Pirazzini, Gianfranco Giorgi, Brunella Del Re

Subject: Biology And Life Sciences, Life Sciences Keywords: Radiofrequency electromagnetic field; DNA methylation; Neuroblastoma; LINE-1; Alu; ribosomal DNA

Online: 27 April 2023 (04:12:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1258.v1

Genetic. Phenotypic and Clinical Heterogeneity of NPM1-Mutant Acute Myeloid Leukemias

Ugo Testa, Elvira Pelosi, Germana Castelli

Subject: Biology And Life Sciences, Life Sciences Keywords: acute myeloid leukemia; genetic classification; mutational profiling; transcriptome analysis; prognostic stratification; clonal evolution

Online: 17 May 2023 (14:36:39 CEST)

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Preprint REVIEW | doi:10.20944/preprints202311.1865.v1

Molecular Mechanisms Involved in the Occurrence and Progression of Long COVID and Associated Analysis Techniques

Adela Constantinescu-Bercu, Andrei Lobiuc, Olga Adriana Caliman Sturdza, Radu Oita, Monica Iavorschi, Naomi Paval, Iuliana Soldanescu, Mihai Dimian, Mihai Covasa

Subject: Biology And Life Sciences, Life Sciences Keywords: immunity; inflammation; SARS-CoV-2; gene expression; sequencing

Online: 29 November 2023 (10:54:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202305.0528.v1

Comparative Effects of Botanical Powders in Controlling Sitophilus zeamais (Maize Weevils) in Stored Maize (Zea mays L.)

Ojuolape Damilola Olanrewaju, Jennifer Chinenye Opara, Ofodile Peter Nwite, Usman Umoru, Mukhtar Sidi Aliyu

Subject: Biology And Life Sciences, Life Sciences Keywords: Botanicals; Sitophilus zeamais; Maize; Storage; Synthetic Pesticide; Pest; Efficacy

Online: 8 May 2023 (10:56:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1067.v1

Designing a Multi-Epitope Subunit Vaccine against VP1 Major Coat Protein of JC Polyomavirus

Sukhada Kanse, Mehak Khandelwal, Rajan Kumar Pandey, Manoj Khokhar, Neetin S Desai, Bajarang Kumbhar

Subject: Biology And Life Sciences, Life Sciences Keywords: Progressive Multifocal Encephalopathy (PML); JC Polyomavirus; VP1 protein; Immunoinformatics; molecular dynamics simulation

Online: 27 April 2023 (10:02:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0761.v1

A Narrative Review on a Deep Understanding of the Dominant Physiological Energy Systems in Basketball and the Importance of Specificity and Uniqueness in Measuring Basketball Players

Asaf Shalom, Roni Gottlieb, Pedro E. Alcaraz, Julio Calleja-González

Subject: Biology And Life Sciences, Life Sciences Keywords: physiology; basketball; testing; explosive; unique; specific movements

Online: 12 October 2023 (05:32:49 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0538.v1

The Roles of Ral-Interacting Protein 76 and Vascular Endothelial Growth Factor in the Ovarian Corpus Luteum and Tumor Microenvironment

Tae-Hee Min, Seunghyung Lee

Subject: Biology And Life Sciences, Life Sciences Keywords: Ral-interacting protein 76; vascular endothelial growth factor; small GTPase; hypoxia-inducible factor-1; corpus luteum; tumor

Online: 19 April 2023 (04:10:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1698.v1

Comparison of Deeply Versus Mildly Invasive Mesothelioma Proteomes Identifies Potential Links Between Mitochondrial Acadvl Expression and Biomarkers of Aggressiveness

Daniel L. Pouliquen, Joanna Kopecka, Stéphanie Blandin, Alice Boissard, Cécile Henry, Chiara Riganti, Catherine Guette

Subject: Biology And Life Sciences, Life Sciences Keywords: Cancer; Aggressiveness; Mitochondria; Acadvl; Fatty acid beta oxidation; Biomarker; Proteomics; Malignant mesothelioma

Online: 24 May 2023 (05:06:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1543.v1

Recruitment of the Histone Variant macroH2A1 to the Pericentric Region Occurs upon Chromatin Relaxation and Is Responsible for Major Satellite Transcriptional Regulation

Ludmila Recoules, Nicolas Tanguy Le Gac, Fatima Moutahir, Kerstin Bystricky, Anne-Claire Lavigne

Subject: Biology And Life Sciences, Life Sciences Keywords: chromatin; macroH2A1; pericentromere

Online: 24 July 2023 (09:28:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.1376.v1

Morphometrical and Scanning Electron Microscopic Investigations on the Rahmani Sheep’s Tongue: Adaptive Strategies for Feeding Behaviors

Fatma A. Madkour, Soha A. Soliman, Hanan H. Abd-elhafeez, Ahmed Elmansi

Subject: Biology And Life Sciences, Life Sciences Keywords: papillae; salivary glands; SEM; sheep; tongue

Online: 19 June 2023 (16:12:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1251.v1

mTOR and Gut Microbiota in Various Disorders：mechanisms and Potential Drugs

Yuan Gao, Tian Tian

Subject: Biology And Life Sciences, Life Sciences Keywords: mTOR; gut microbes; metabolites; therapy

Online: 16 June 2023 (14:02:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0152.v1

Temperature-Humidity Dependent Wind Effects on Physiological Heat Strain of Moderately Exercising Individuals Reproduced by the Universal Thermal Climate Index UTCI

Peter Bröde, Bernhard Kampmann

Subject: Biology And Life Sciences, Life Sciences Keywords: heat stress; air temperature; humidity; wind; heat wave; electrical fan; index

Online: 3 May 2023 (12:52:02 CEST)

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Increasing wind speed alleviates physiological heat strain, however, health policies have advised against using ventilators or fans under heat wave conditions with air temperatures above the typical skin temperature of 35 °C. Recent research, mostly with sedentary participants, suggests mitigating effects of wind at even higher temperatures depending on the humidity level. Our study aimed at exploring and quantifying, whether such results are transferable to moderate exercise levels, and whether the Universal Thermal Climate Index UTCI reproduces those effects. We measured heart rates, core and skin temperatures and sweat rates in 198 laboratory experiments completed by five young, semi-nude, heat-acclimated, moderately exercising males walking the treadmill with 4 km/h on the level for three hours under widely varying temperature-humidity combinations and two wind conditions. We quantified the cooling effect of increasing wind speed from 0.3 to 2 m/s by fitting generalized additive models predicting the physiological heat stress responses depending on ambient temperature, humidity and wind speed. We then compared the observed wind effects to the assessment performed by UTCI. Increasing wind speed lowered physiological heat strain for air temperatures below 35 °C, but also for higher temperatures with humidity levels above 2 kPa water vapour pressure concerning heart rate and core temperature, and 3 kPa concerning skin temperature and sweat rate, respectively. UTCI assessment of wind effects correlated positively with the observed changes in physiological responses, showing the closest agreement (r=0.9) for skin temperature and sweat rate, where wind is known for elevating the relevant convective and evaporative heat transfer. These results demonstrate the potential of UTCI for adequately assessing sustainable strategies for heat stress mitigation involving fans or ventilators depending on temperature and humidity for moderately exercising individuals.

Preprint REVIEW | doi:10.20944/preprints202403.1638.v1

Short-chain Fatty Acids and Human Health: from Metabolic Pathways to Current Therapeutic Implication

Sonia Facchin, Luisa Bertin, Erica Bonazzi, Greta Lorenzon, Caterina De Barba, Brigida Barberio, Fabiana Zingone, Daria Maniero, Marco Scarpa, Cesare Ruffolo, Imerio Angriman, Edoardo V. Savarino

Subject: Biology And Life Sciences, Life Sciences Keywords: intestinal microbiota; short-chain fatty acids (SCFAs); dietary fiber fermentation; gastrointestinal and metabolic health; therapeutic implication

Online: 27 March 2024 (06:39:18 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0355.v1

Application and Prospects of Long Read Sequencers for Preimplantation Genetic Testing

Tasuku Mariya, Yui Shichiri, Yuri Murase, Hidehito Inagaki, Hiroki Kurahashi

Subject: Biology And Life Sciences, Life Sciences Keywords: long read sequencer; sequel sequencer; nanopore sequencer; preimplantation genetic testing

Online: 4 January 2024 (09:53:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1028.v1

Influence of the Human Field of View on Visual and Non-visual Quantities in Indoor Environments

Johannes Zauner, Kai Broszio, Karin Bieske

Subject: Biology And Life Sciences, Life Sciences Keywords: Field of View; FOV; non-visual effect of light; non-image-forming effect of light; NIF; ipRGC; melanopsin; corneal illuminance; retinal illuminance; head orientation; gaze

Online: 14 June 2023 (09:28:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.0957.v1

The Application of Zebrafish Model in the Study of Idiopathic Scoliosis

Keyi Ren, Mijuan Shi, Yingyin Cheng, Wanting Zhang, Yaping Wang, Xiao-Qin Xia

Subject: Biology And Life Sciences, Life Sciences Keywords: adolescent idiopathic scoliosis; animal model; zebrafish; cilia; cerebrospinal fluid

Online: 14 July 2023 (09:04:41 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202310.1820.v1

Acupoints, Channels and Organs

Mikhail Teppone

Subject: Biology And Life Sciences, Life Sciences Keywords: Traditional Chinese Medicine; TCM; Integrative Medicine; Acupoints; Channels; Collaterals

Online: 27 October 2023 (14:45:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.1220.v1

PGC-1α Regulates the Interplay between Oxidative Stress, Senescence and Autophagy in the Aging Retina Important in Age-Related Macular Degeneration

Iswariyaraja Sridevi Gurubaran, Cezary Watala, Joanna Kostanek, Joanna Szczepanska, Elzbieta Pawlowska, Kai Kaarniranta, Janusz Blasiak

Subject: Biology And Life Sciences, Life Sciences Keywords: age-related macular degeneration; aging retina; peroxisome proliferator-activated receptor gamma coactivator 1-alpha; PGC-1 AMD; cellular senescence; autophagy; oxidative stress

Online: 18 July 2023 (11:09:42 CEST)

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Preprint REVIEW | doi:10.20944/preprints202307.1283.v2

The Future of Stress Management: Integration Smartwatches and HRV Technology

Ravinder Jerath, Mohammad Syam, Shajia Ahmed

Subject: Biology And Life Sciences, Life Sciences Keywords: smartwatch; stress; wearable device; heart rate variability; comparative analysis

Online: 25 July 2023 (09:28:26 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1438.v1

The Molecular and Immune Microenvironmental Landscape of Brain Metastases: Implications for Novel Treatment Options

Shangbiao Li, Yuchen Shen, Chengtao Dong, Shengqi Yin, Dong Zhou, Aidong Zhou

Subject: Biology And Life Sciences, Life Sciences Keywords: brain metastasis; tumor microenvironment; targeted therapy; immune therapy

Online: 20 June 2023 (11:56:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202307.0580.v1

Abundance and Diversification of Repetitive Elements in Decapoda Genomes

Christelle Rutz, Arnaud Kress, Ljudevit Luka Boštjančić, Lena Bonassin, Caterina Francesconi, Dorine Merlat, Kathrin Theissinger, Odile Lecompte

Subject: Biology And Life Sciences, Life Sciences Keywords: transposable elements; satellite DNA; crustaceans; annotation; evolution; genome size; library

Online: 10 July 2023 (10:47:25 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0370.v1

How Did Life Emerge in Chemically Complex Messy Environments?

Kenji Ikehara

Subject: Biology And Life Sciences, Life Sciences Keywords: GADV hypothesis; origin of life; protein 0th-order structure; origin of protein; [GADV]-microsphere; origin of gene: the core life system

Online: 28 June 2022 (03:41:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1196.v1

Integrating (Nutri-)Metabolomics Into the One Health Tendency – The Key for Personalized Medicine Advancement

Ionela Hotea, Catalin Sirbu, Ana-Maria Plotuna, Emil Tîrziu, Corina Badea, Adina Berbecea, Monica Dragomirescu, Isidora Radulov

Subject: Biology And Life Sciences, Life Sciences Keywords: metabolomics; nutrimetabolomics; One Health; public health; personalized medicine.

Online: 16 June 2023 (08:29:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0787.v1

Impact of Climate Change on Phenology of Entomophilous Plants and Honey Bee (Apis mellifera L.)

Danuta Romanovskaja, Eugenija Baksiene, Almantas Razukas

Subject: Biology And Life Sciences, Life Sciences Keywords: entomophilous plants; phenophases; honeybee; climate change

Online: 11 May 2023 (04:47:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2008.v1

Radical-Driven Methane Formation in Humans Evidenced by Exogenous Isotope-Labeled DMSO and Methionine

Frank Keppler, Mihály Boros, Daniela Polag

Subject: Biology And Life Sciences, Life Sciences Keywords: endogenously produced methane; reactive oxygen species; methyl radicals; dimethyl sulfoxide; methyl donors; stable carbon and hydrogen isotope labelling; oxidative stress; antioxidant defense system

Online: 29 May 2023 (10:27:42 CEST)

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Methane (CH4), produced endogenously in animals and plants, has recently been suggested to play a role in cellular physiology, potentially influencing the signaling pathways and regulatory mechanisms involved in nitrosative and oxidative stress responses. In addition, it has been proposed that supplementation of CH4 to organisms may be beneficial for the treatment of several diseases, including ischemia, reperfusion injury, and inflammation. However, it is still unclear if and how CH4 is produced in mammalian cells without the help of microorganisms, and how CH4 might be involved in physiological processes in humans. In this study, we provide first proof of the principle that CH4 is formed non-microbially in the human body by applying isotopically labeled methylated sulfur compounds, such as dimethyl sulfoxide (DMSO) and methionine, as carbon precursors to confirm cellular CH4 formation. A volunteer applied isotopically labeled (2H and 13C) DMSO on the skin, orally, and to blood samples. Monitoring of stable isotope values of CH4 convincingly showed the conversion of the methyl groups, as isotopically labeled CH4 was formed during all experiments. Based on these results, we consider several hypotheses about endogenously formed CH4 in humans, including physiological aspects and stress responses involving reactive oxygen species (ROS). While further and broader validation studies are needed, the results may unambiguously serve as a proof of concept for the endoge-nous formation of CH4 in humans by a radical-driven process. Furthermore, these results might encourage follow up studies to decipher the potential physiological role of CH4 and its bioactiv-ity in humans in more detail. Of particular importance is the potential to monitor CH4 as an ox-idative stress biomarker if the observed large variability of CH4 in breath air is an indicator for physiological stress responses and immune reactions. Finally, the potential role of DMSO as a radical scavenger to counteract oxidative stress caused by ROS might be considered in the health sciences. DMSO has already been investigated for many years, but its potential positive role for medical use remained highly uncertain.

Preprint REVIEW | doi:10.20944/preprints202308.0847.v1

MicroRNA-34 Family in Cancers: Role, Mechanism, and Therapeutic Potential

Junjiang Fu, Saber Imani, Mei-Yi Wu, Ray-Chang Wu

Subject: Biology And Life Sciences, Life Sciences Keywords: microRNA; miR‐34; tumor suppressor; miRNA therapy

Online: 10 August 2023 (10:53:25 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1708.v1

Cytoplasmic Membrane Homeostasis is Important for the Pathogenicity of Pathogenic Fungi

Yuejin Peng, Bin Chen

Subject: Biology And Life Sciences, Life Sciences Keywords: Cytoplasmic membrane homeostasis; Pathogenicity; Pathogenic fungi; Membrane lipid metabolism; Membrane curvature; Transmembrane protein; Cell wall components; Cytoskeleton; Growth and development; Host infection

Online: 24 May 2023 (10:38:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.1281.v1

Microcirculation in Hypertension: A Therapeutic Target To Prevent Cardiovascular Disease?

Rizzoni Damiano, Claudia AGABITI ROSEI, Gianluca E.M. Boari, Maria Lorenza Muiesan, Carolina De Ciuceis

Subject: Biology And Life Sciences, Life Sciences Keywords: microcirculation; peripheral circulation; remodelling; small resistance arteries; vascular biology

Online: 19 June 2023 (04:11:00 CEST)

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Arterial hypertension is a common condition worldwide and an important risk factor for cardio- and cerebrovascular events, renal diseases as well as microvascular eye diseases. Established hypertension leads to chronic vasoconstriction of small arteries as well as to decreased lumen diameter and thickening of the arterial media or wall with a consequent increased media-to-lumen ratio (MLR) or wall-to-lumen ratio (WLR). This process, defined as vascular remodeling, was first demonstrated in small resistance arteries isolated from subcutaneous biopsies and measured by micromyography, and this is still considered the gold-standard method for the assessment of structural alteration in small resistance arteries; however microvascular remodeling seems to represent a generalized phenomenon. An increased MLR may impair organ flow reserve, being relevant in the maintenance and, probably, also in the progressive worsening of hypertensive disease, as well as in the development of hypertension-mediated organ damage/cardiovascular events, possessing, therefore, a prognostic relevance. New, non-invasive techniques, such as Scanning Laser Doppler Flowmetry or Adaptive Optics, are presently under development, focusing mainly on the evaluation of WLR in retinal arterioles; recently, also retinal microvascular WLR was demonstrated to have a prognostic impact in term of cardio- and cerebrovascular events. A rarefaction of capillary network has also been reported in hypertension which may contribute to flow reduction and impairment in oxygen delivery to different tissues. These microvascular alterations seem to represent an early step in hypertension-mediated organ damage since they might concur to microvascular angina, stroke, and renal dysfunction. In addition, they could be a marker useful in monitoring the beneficial effect of antihypertensive treatment. Also conductance arteries may be affected by a remodeling process in hypertension, and a cross-talk may exist between structural changes in the small and large arteries. The review will address the possible relations between structural microvascular alterations and hypertension-mediated organ damage and their potential improvement with antihypertensive treatment.

Preprint REVIEW | doi:10.20944/preprints202310.1337.v1

Diffusion Idiopathic Skeletal Hyperostosis (DISH), a Link between Gut Microbiota and Spinal Degenerative Diseases

Iddrisu Ibrahim, Joseph Ayariga, Junhuan Xu, Robertson K. Boakai, Olufemi Ajayi

Subject: Biology And Life Sciences, Life Sciences Keywords: DISH; Gut microbiota; Skeletal Hyperostosis; Spondylitis; Irritable Bowel Syndrome; Inflammatory Bowel Disease; Intervertebral Disc Degeneration

Online: 20 October 2023 (12:28:17 CEST)

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The interplay between Diffuse Idiopathic Skeletal Hyperostosis (DISH) and the gut microbiota represents a compelling area of research, offering insights into the relationship between spinal degenerative diseases and the gut's microbial inhabitants. This comprehensive review seeks to unravel the complex connection, shedding light on its implications for human health and potential therapeutic avenues. A crucial section dives into the world of gut microbiota (GM), exploring its impact on human health, featuring its defining characteristics, and highlighting its role in metabolism, immunomodulation, host interaction, and how its composition changes over time. The subsequent portion delves into the relationship between microbiome composition and pathological bone development, including the link between Intervertebral Disc Degeneration (IDD) and gut inflammation, emphasizing how gut inflammation and GM dysbiosis play pivotal roles. Further dissecting the interconnections, various axes of influence, including the gut-spine axis, gut-bone axis, gut-joint axis, gut-disc axis (specific to DISH), gut-ligament axis, and gut-muscle axis, are discussed. Of particular interest is the section on the impact of GM on DISH-derived pain, addressing a critical aspect of the condition's manifestation. The review discusses the potential of GM modulation as a treatment for DISH, providing hope for therapeutic advancements. Additionally, it highlights the positive impact of lifestyle interventions, such as dietary modifications, sleep optimization, and exercise regimens, in mitigating GM dysbiosis and potentially improving DISH outcomes. Lastly, the intriguing prospect of fecal microbiome transplant and its implications for DISH management is examined, opening new avenues for research and therapeutic interventions. In conclusion, this comprehensive review underscores the intricate interplay between DISH and the gut microbiota, offering a fresh perspective on the impact of GM on spinal degenerative diseases. It emphasizes the potential for innovative treatments, such as GM modulation and lifestyle interventions, and presents fecal microbiome transplant as an exciting area for future exploration.

Preprint REVIEW | doi:10.20944/preprints202401.1912.v1

Back to the Future of Metabolism – Advances in the Discovery and Characterization of Unknown Biocatalytic Functions and Pathways

Roland Wohlgemuth

Subject: Biology And Life Sciences, Life Sciences Keywords: Enzyme functions; domains of unknown functions; central metabolic pathways; biosynthesis; biosynthetic gene clusters; cryptic pathways; orphan pathways; silent pathways; metabolite repair enzymes

Online: 26 January 2024 (11:07:28 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.0388.v1

Association of TP53 Single Nucleotide Polymorphisms with Prostate Cancer in a Racially Diverse Cohort of Men

Allison Duncan, Darryl Nousome, Randy Ricks, Huai-Ching Kuo, Lakshmi Ravindranath, Albert Dobi, Jennifer Cullen, Shiv Srivastava, Gregory Chesnut, Gyorgy Petrovics, Indu Kohaar

Subject: Biology And Life Sciences, Life Sciences Keywords: prostate cancer; SNP; TP53; Pro47Ser; Arg72Pro; African American

Online: 17 April 2023 (03:41:25 CEST)

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Biology and Life Sciences