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Preprint ARTICLE | doi:10.20944/preprints202306.0642.v1

Adverse Crosstalk Between Extracellular Matrix Remodeling and Ferroptosis in Basal Breast Cancer

Christophe Desterke, Emma Cosialls, Yao Xiang, Rima Elhage, Clémence Duruel, Yunhua Chang-Marchand, Ahmed Hamaï

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: basal breast cancer; extracellular matrix remodeling; ferroptosis; transcriptome; text mining

Online: 8 June 2023 (11:21:03 CEST)

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(1) Background: Breast cancer is a frequent heterogeneous disorder diagnosed in woman and is a high cause of mortality of them in reason to rapid metastasis and disease recurrence. Ferroptosis can inhibit breast cancer cell growth, improve the sensitivity of chemotherapy and radiotherapy and inhibit distant metastases so potentially acts on tumor micro-environment; (2) Methods: Ferroptosis/Extracellular matrix remodeling literature text-mining results were integrated in breast cancer transcriptome cohort according their distant relapse free survival (DRFS) under adjuvant therapy (anthracyclin+taxanes) and also in MDA-MB-231 transcriptome functional experiments with ferroptosis activations (GSE173905); (3) Results: Ferroptosis/Extracellular matrix remodeling text-mining identified 910 associated genes in at list 10 articles. Univariate Cox analyses censored on breast cancer (GSE25066) selected 252 individual significant genes and 171 of them found with an adverse expression. Functional enrichment of these 171 adverse genes predicted basal breast cancer signatures. By text-mining some ferroptosis significant adverse selected genes shared citations in domain of ECM remodeling such as: TNF, IL6, SET, CDKN2A, EGFR, HMGB1, KRAS, MET, LCN2, HIF1A, TLR4. A molecular score based on expression the eleven genes was found predictive of worst prognosis breast cancer at univariate level: basal subtype, short DRFS, high grade values 3 and 4, estrogen and progesterone receptors negative and nodal stages 2 and 3. This eleven gene signature was validated as regulated by ferroptosis inductors (erastin and RSL3) in triple negative breast cancer cellular model MDA-MB-231.; (4) Conclusions: Crosstalk between ECM remodeling-Ferroptosis functionalities allowed to define a molecular score which have been characterized as an independent adverse parameter in prognosis of breast cancer patients. Gene signature of this molecular score have been validated to be regulated by erastin/RSL3 ferroptosis activators. This molecular score could be promising to evaluate ECM impact of ferroptosis target therapies in breast cancer.

Preprint ARTICLE | doi:10.20944/preprints202306.0501.v1

MicroRNA miR-274-5p Controls Melanotic Mass Formation and Developmental Growth by Regulating the Found-in-Neurons-JNK Signaling Axis in Drosophila

Hee Kyung Kim, Chae Jeong Kim, Daegyu Jang, Do-Hwan Lim

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Drosophila; miR-274; melanotic mass; growth; JNK signaling; JAK/STAT signaling; found-in-neurons

Online: 7 June 2023 (07:12:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0464.v1

Physiological Calcium Phosphate Management in Two Biofluids

Deanna Nelson

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Calcium phosphate; calciprotein particles; nanoclusters; intrinsically disordered proteins; crystal toxicity; milk; serum

Online: 6 June 2023 (14:19:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0177.v1

Ferroptosis in Cancer Progression

Rongyu Zhang, Jinghong Chen, Saiyang Wang, Wenlong Zhang, Quan Zheng, Rong Cai

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: ferroptosis; cancer; EMT; angiogenesis; metastasis

Online: 2 June 2023 (10:12:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202306.0170.v1

Vascular Dysfunctions Contribute to the Long-term Cognitive Deficits Following COVID-19

zahra shabani, Jialing Liu, hua su

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Long-COVID-19; SARS-CoV-2; Endothelial cells; Cognitive dysfunction; Blood Brain Barrier; Neuro-inflammation

Online: 2 June 2023 (09:38:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202306.0109.v1

MCAK Inhibitors Induce Aneuploidy in Triple Negative Breast Cancer Models

John C Smith, Stefan Husted, Jay Pilrose, Stephanie C Ems-McClung, Jane R Stout, Richard L Carpenter, Claire E Walczak

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: triple negative breast cancer; MCAK/KIF2C; aneuploidy; chromosomal instability; drug discovery

Online: 1 June 2023 (15:47:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.2155.v1

Budesonide Analogues Preserve Stem Cell Pluripotency and Delay 3D Gastruloid Development

Filomena Amoroso, Eduardo Ibello, Federica Saracino, Federica Cermola, Giovanna Ponticelli, Enrica Scalera, Francesca Ricci, Gino Villetti, Gilda Cobellis, Gabriella Minchiotti, Eduardo Jorge Patriarca, Dario De Cesare, Cristina D'Aniello

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: drug toxicity; budesonide analogues; stem cells; pluripotency exit; 3D gastruloids

Online: 30 May 2023 (13:48:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.2122.v1

The Interplay between Histone Modifiers and p53 in Regulating Gene Expression

Hyun-Min Kim, Xiaoyu Zheng, Ethan Lee

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Histone modifications; p53; cancer; gene regulation; chromatin structure

Online: 30 May 2023 (11:14:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1655.v1

Melanin Journey from Melanocytes to Keratinocytes: Uncovering the Molecular Mechanisms of Melanin Transfer and Processing

Liliana Bento-Lopes, Luís C. Cabaço, João Charneca, Matilde V. Neto, Miguel C. Seabra, Duarte C. Barral

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Melanin; Melanocyte; Keratinocyte; Melanosome; Melanocore; Melanokerasome; Mlelanin transfer; Melanin processing; Melanin polarization

Online: 23 May 2023 (12:21:10 CEST)

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Skin pigmentation ensures efficient photoprotection and relies on the pigment melanin, which is produced by epidermal melanocytes and transferred to surrounding keratinocytes. While the molecular mechanisms of melanin synthesis and transport in melanocytes are now well characterized, much less is known about melanin transfer and processing within keratinocytes. Over the past few decades, distinct models have been proposed to explain how melanin transfer occurs at the cellular and molecular levels. However, this remains a debated topic as up to four different models have been proposed, with evidence presented supporting each. Here, we review the current knowledge on the regulation of melanin exocytosis, internalization, processing and polarization. Regarding the different transfer models, we discuss how these might co-exist to regulate skin pigmentation under different conditions, i.e. constitutive and facultative skin pigmentation or physiological and pathological conditions. Moreover, we discuss recent evidence that sheds light on the regulation of melanin exocytosis by melanocytes and internalization by keratinocytes, as well as how melanin is stored within these cells in a compartment that we proposed to be named melanokerasome. Finally, we review the state of the art on the molecular mechanisms that lead to melanokerasome positioning above the nuclei of keratinocytes, forming supranuclear caps that shield the nuclear DNA from UV radiation. Thus, we provide a comprehensive overview of the current knowledge on the molecular mechanisms regulating skin pigmentation, from melanin exocytosis by melanocytes and internalization by keratinocytes, to processing and polarization within keratinocytes. A better knowledge of these molecular mechanisms will clarify long lasting questions in the field that are crucial for the understanding of skin pigmentation and can shed light on fundamental aspects of organelle biology. Ultimately, this knowledge can lead to novel therapeutic strategies to treat hypo- or hyper-pigmentation disorders, which have a high socio-economic burden to patients and healthcare systems worldwide, as well as having cosmetic applications.

Preprint ARTICLE | doi:10.20944/preprints202305.1465.v1

CD10-Bound Human Mesenchymal Stem/Stromal Cell-Derived Exosomes Possess Immunomodulatory Cargo and Maintain Cartilage Homeostasis under Inflammatory Conditions

Dimitrios Kouroupis, Lee D. Kaplan, Johnny Huard, Thomas M. Best

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Mesenchymal Stem/Stromal Cells (MSC); Synovium; Infrapatellar fat pad (IFP); CD10 (neprilysin); PRG4 (lubricin); Exosomes; Immunomodulation; Pain; Chondroprotection; Inflammatory Joint Diseases

Online: 22 May 2023 (05:17:14 CEST)

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The onset and progression of human inflammatory joint diseases are strongly controlled by the activation of resident synovium/infrapatellar fat pad (IFP) pro-inflammatory and pain-transmitting signaling. We recently reported that intra-articularly injected IFP-derived Mesenchymal Stem/Stromal Cells (IFP-MSC) acquire a potent immunomodulatory phenotype and actively degrade Substance P (SP) via neutral endopeptidase CD10 (neprilysin). Our hypothesis is that IFP-MSC robust immunomodulatory therapeutic ef-fects are largely exerted via their CD10-bound exosomal secretome (IFP-MSC EXOs) by attenuating synoviocyte pro-inflammatory activation and articular cartilage degradation. Herein, IFP-MSC EXOs were isolated from CD10High- and CD10Low-expressing IFP-MSC cultures and their exosomal miRNA cargo was assessed using multiplex methods. Functionally, we interrogated the effect of CD10High and CD10Low EXOs on stimulated by inflammatory/fibrotic cues synoviocytes monocultures and cocultures with IFP-MSC de-rived chondropellets. Finally, CD10High EXOs were tested in vivo for their therapeutic capacity in an animal model of acute synovitis/fat pad fibrosis. Our results showed that CD10High and CD10Low EXOs possess distinct miRNA profiles. Reactome analysis of miRNAs highly present in exosomes showed their involvement in the regulation of six gene groups, particularly the immune system. Stimulated synoviocytes exposed to IFP-MSC EXOs demonstrated significantly reduced proliferation and altered inflammation-related molecular profiles compared to control stimulated synoviocytes. Importantly, CD10High EXOs treatment of stimulated chondropellets/synoviocytes cocultures indicated a significant chondroprotective effects. Therapeutically, CD10High EXOs treatment resulted in robust chondroprotective effects by retaining articular cartilage structure/composition and PRG4 (lubricin)-expressing cartilage cells in the animal model of acute synovitis/IFP fibrosis. Our study suggests that CD10High EXOs possess immuno-modulatory miRNA attributes with strong chondroprotective/anabolic effects for articular cartilage in vivo. The results could serve as a foundation for EXOs-based therapeutics for the resolution of detrimental aspects of immune-mediated inflammatory joint changes associated with conditions such as osteoarthritis (OA).

Preprint ARTICLE | doi:10.20944/preprints202305.1252.v1

Impact of the Overexpression of the Tyrosine Kinase Receptor RET in the Hematopoietic Potential of Induced Pluripotent Stem Cells (iPSCs)

Paul Marcoux, Jusuf Imeri, Christophe Desterke, Theodoros Latsis, Diana Chaker, Patricia Hugues, Annelise Bennaceur-Griscelli, Ali G Turhan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: iPSCs; HSCs; RET; Hematopoietic differentiation; CD34

Online: 17 May 2023 (13:49:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1184.v1

Parathyroid Hormone Related Protein (PTHrP)-Associated Molecular Signatures in Cell Signaling during Tissue Differentiation and in Non-tumoral Diseases

Mariangela Librizzi, Flores Naselli, Giulia Abruscato, Claudio Luparello, Fabio Caradonna

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cell biology; gene expression; adipose tissue; bone; cartilage; intestine; kidney; liver; lung; pancreas; skin

Online: 17 May 2023 (04:41:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.1093.v1

CDC6 as A Key Inhibitory Regulator of CDK1 Activation Dynamics and The Timing of Mitotic Entry and Progression

Mohammed EL DIKA, Damian DUDKA, Malgorzata KLOC, Jacek Z. KUBIAK

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Timing of mitosis; Cell cycle; CDC6; CDK1; cyclins; CDC25; Xic1; Mitotic entry

Online: 16 May 2023 (05:10:23 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.1059.v1

Corneal Edema in Inducible Slc4a11 Knockout Is Initiated by Mitochondrial Superoxide Induced Src Kinase Activation

Diego G Ogando, Edward T Kim, Shimin Li, Joseph A. Bonanno

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: corneal enendothelial dystrophy; Slc4a11; barrier function; lactate transporters; Src kinase; oxidative stress; Visomitin

Online: 15 May 2023 (15:50:03 CEST)

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Purpose: Inducible Slc4a11 KO leads to corneal edema by disruption of the pump and barrier functions of the corneal endothelium (CE). The loss of Slc4a11 NH3-activated mitochondrial uncoupling leads to mitochondrial membrane potential hyperpolarization-induced oxidative stress. The goal of this study is to investigate the link between oxidative stress and failure of pump and barrier functions and test different approaches to revert the process. Methods: Mice homozygous for Slc4a11 Flox and Estrogen receptor –Cre Recombinase fusion protein alleles at 8 weeks of age were fed Tamoxifen (Tm) enriched chow (0.4 g/Kg) for 2 weeks, and controls were fed normal chow. During the initial 14 days, Slc4a11 expression, corneal thickness (CT), stromal [lactate], Na+-K+ ATPase activity, mitochondrial superoxide levels, expression of lactate transporters, and activity of key kinases were assessed. In addition, barrier function was assessed by fluorescein permeability, ZO-1 tight junction integrity, and cortical cytoskeleton F-actin morphology. Results: Tm induced a rapid decay in Slc4a11 expression that was 84% complete at 7 days and 96% at 14 days of treatment. Superoxide levels increased significantly by day 7; CT and fluorescein permeability by day 14. Tight junction ZO-1 distribution and cortical cytoskeleton were disrupted at day 14 concomitant with decreased expression of Cldn1 yet an increase in tyrosine phosphorylation. Stromal lactate increased by 60%, Na+-K+ATPase activity decreased by 40%, and expression of lactate transporters MCT2 and MCT4 significantly decreased, but MCT1 was unchanged at 14 days. Src kinase was activated but not Rock, PKCα, JNK, or P38Mapk. Mitochondrial antioxidant Visomitin (SkQ1, mitochondrial targeted antioxidant) or Src kinase inhibitor eCF506 significantly slowed the increase in CT, with concomitant decreased stromal lactate retention, improved barrier function, reduced Src activation and Cldn1 phosphorylation, and rescued MCT2 and MCT4 expression. Conclusions: Slc4a11 KO-induced CE oxidative stress triggered increased Src kinase activity that results in perturbation of pump components and barrier function of the CE.

Preprint ARTICLE | doi:10.20944/preprints202305.0938.v1

Differentiation Capacity of Porcine Skeletal Muscle-Derived Stem Cells As Intermediate Species Between Mice and Humans

Tetsuro Tamaki, Toshiharu Natsume, Akira Kato, Nobuyuki Nakajima, Kosuke Saito, Tsuyoshi Fukuzawa, Masayoshi Otake, Satoko Enya, Akihisa Kangawa, Takeshi Imai, Miyu Tamaki, Yoshiyasu Uchiyama

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: micro-mini pig; large animal experiment; GFP-transgenic pig; multipotent stem cells; skeletal muscle; nerve-muscle regeneration.

Online: 12 May 2023 (11:18:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0570.v1

Human Sperm Head Vacuoles Are Related to Nuclear-Envelope Invaginations

María José Gómez-Torres, Javier Luna-Romero, Pedro José Fernández-Colom, Jon Aizpurua, Manuel Avilés, Alejandro Romero

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: human sperm; vacuoles; ultrastructure; morphology; immunocytochemistry; TEM

Online: 9 May 2023 (05:10:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0538.v1

Genetic Targeting of the dTORC1 Inhibitor, dSAMTOR, during Drosophila Aging: A Tissue-specific Pathology

Stamatia A. Katarachia, Sophia P. Markaki, Athanassios D. Velentzas, Dimitrios J. Stravopodis

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Aging; Betaine; dBHMT; Drosophila; dSAMTOR; dTORC1; Kinase; Methionine; SAM

Online: 8 May 2023 (12:06:37 CEST)

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mTORC1 regulates mammalian cell metabolism and growth, in response to diverse environmental stimuli. Nutrient signals control the localization of mTORC1 onto lysosome-surface scaffolds that are critically implicated in its amino acid-dependent activation. Arginine, Leucine and S-Adenosyl-Methionine (SAM) can serve as major mTORC1-signaling activators, with SAM binding to SAMTOR (SAM + TOR), a fundamental SAM sensor, abolishing protein’s (SAMTOR’s) inhibitory action(s) against mTORC1, thereby releasing its (mTORC1) kinase activity. Given the lack of knowledge regarding the role of SAMTOR in invertebrates, we have, in silico, identified the Drosophila SAMTOR homologue (dSAMTOR) and have, herein, genetically targeted it, through utilization of the GAL4/UAS transgenic tool. Survival profiles and negative geotaxis patterns were examined both in control and dSAMTOR-downregulated adult flies, during aging. One of the two gene-targeted schemes resulted in lethal phenotypes, whereas the other one caused rather moderate pathologies in most tissues. Screening of head-specific kinase activities, via PamGene technology application, unveiled the significant upregulation of several kinases, including the dTORC1 characteristic substrate, dp70S6K, in dSAMTOR-downregulated flies, thus strongly supporting the inhibitory dSAMTOR function(s) upon dTORC1/dp70S6K signaling axis in Drosophila-brain settings. Importantly, genetic targeting of the Drosophila BHMT -bioinformatics- counterpart (dBHMT), an enzyme that catabolizes Betaine to produce Methionine (the SAM precursor), led to severe compromises in fly longevities, with glia-, motor neuron- and muscle-specific dBHMT downregulations exhibiting the strongest effects. Abnormalities in wing-vein architectures were also detected in dBHMT-targeted flies, thereby justifying their notably reduced negative geotaxis capacities, herein, observed, mainly in the brain-(mid)gut axis. In vivo adult-fly exposure to clinically relevant doses of Methionine revealed the mechanistic synergism of decreased dSAMTOR and increased Methionine levels in pathogenic longevity, thus rendering (d)SAMTOR an important component in Methionine-associated disorders, including Homocystinuria(s).

Preprint REVIEW | doi:10.20944/preprints202305.0457.v1

New Discoveries on Molecules Inducing Dental Stem Cells Differentiation and Bone Regeneration

Anastasia Ariano, Francesca Posa, Giuseppina Storlino, Giorgio Mori

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Dental stem cells; Mesenchymal Stem Cells; Dental Pulp Stem Cells; Dental Bud Stem Cells; bone regeneration; osteogenic differentiation

Online: 8 May 2023 (05:33:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202305.0154.v1

Cell Aggregation Capability of Clinical Isolates from Candida auris and Candida haemulonii Species Complex

Lívia S. Ramos, Claudia M. Parra-Giraldo, Marta H. Branquinha, André L. S. Santos

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cell-cell interaction; emerging yeasts; drug-resistance; virulence; physicochemical conditions

Online: 3 May 2023 (12:56:06 CEST)

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Preprint REVIEW | doi:10.20944/preprints202305.0034.v1

Gut-on-a-Chip Research for Drug Development: Implications of Chip Design on Preclinical Oral Bioavailability or Intestinal Disease Studies

Joanne M. Donkers, Jamie I. van der Vaart, Evita Van de Steeg

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: organ-on-a-chip; gut-on-a-chip; intestine; in vitro; ex vivo; ADME; oral bioavailability; drug development; microbiome; IBD

Online: 2 May 2023 (01:15:08 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202304.1227.v1

The Putative Neurotransmitter Transporter Blot Regulates F-actin Organization in Drosophila

Guoqing Wang, Zhaoxuan Zhang, Chuanhao Yin, Zhiyi Lv

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Drosophila embryogenesis; SLC; cellularization; Blot; ELMO/DOCK complex

Online: 29 April 2023 (08:02:54 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.1044.v1

Phosphorylation of the CDK Substrate MSCDK Regulates Synaptonemal Complex Disassembly and Meiosis Metaphase I Transition

Mengjing Li, Mingyu Zhang, Hanzhen Li, Gang Lu, Wai-yee Chan, Jinlong Ma, Hongbin Liu, Tao Huang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Meiosis prophase I; Synaptonemal complex (SC) disassembly; Metaphase I transition

Online: 27 April 2023 (04:55:50 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0989.v1

An Emerging Animal Model for Querying the Role of Whole Genome Duplication in Development, Evolution, and Disease

Mara Schvarzstein, Fatema Alam, Muhammad Toure, Judith L Yanowitz

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Whole genome duplication; tetraploidy; polyploidy; aneuploidy; Caenorhabditis; WGD model (List three to ten pertinent keywords specific to the article yet reasonably common within the subject discipline.)

Online: 26 April 2023 (12:43:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0982.v1

Molecular Mechanisms Driven by MT4-MMP in Cancer Progression

Emma Muñoz-Sáez, Natalia Moracho, Ana I. R. Learte, Alice Collingnon, Alicia G. Arroyo, Agnés Noel, Nor Eddine Sounni, Cristina Sánchez-Camacho

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: MT4-MMP; MMP-17; development; vasculature; tumor; cancer cell; malignancy; therapy; bi-omarker

Online: 26 April 2023 (11:19:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0969.v1

Insulin-Degrading Enzyme Targeted to Mitochondria: A Proteomic Study

Ayse Yilmaz, Chiara Guerrera, Emmanuelle Waecke-Enée, Joanna Lipecka, Barbara Bertocci, Peter van Endert

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Mitochondrion; respiratory chain; mitochondrial translation; chaperone

Online: 26 April 2023 (07:40:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202304.0790.v1

A Glimpse into Chromatin Organization and Nuclear Lamina Contribution in Neuronal Differentiation

Salvatore Martino, Pietro Salvatore Carollo, Viviana Barra

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: chromatin organization; nuclear lamina; epigenetics; neuronal differentiation

Online: 23 April 2023 (08:09:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0681.v1

Functional Activity of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes on a Mouse Renal Subcapsular Xenograft Model

Elena Chepeleva, Sophia Pavlova, Nataliya Bgatova, Alexander Volkov, Galina Kazanskaya, David Sergeevichev

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cardiomyocytes; induced pluripotent stem cells; cell therapy; calcium imaging; fluorescence microscopy; transmission electron microscopy

Online: 21 April 2023 (08:10:14 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0432.v1

TRAPPC9 Modulation Regulates IL-1β-Induced Chondrocyte Inflammation

Nazar J Hussein, Andrew L Alejo, Rachel Prior, Hope C. Ball, Fayez F. Safadi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Osteoarthritis; TRAPPC9; Inflammation; Chondrocytes; IL-1β

Online: 17 April 2023 (09:38:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0384.v1

Rapid Generation of Pulmonary Organoids from Induced Pluripotent Stem Cells by Co-Culturing Endodermal and Mesodermal Progenitors for Pulmonary Disease Modelling

Adam Mitchell, Chaowen Yu, Xiangjun Zhao, Laurence Pearmain, Rajesh Shah, Karen Piper Hanley, Timothy Felton, Tao Wang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: pulmonary organoids; induced pluripotent stem cells (iPSCs); anterior foregut endoderm; mesoderm; alveoli epithelial cells; SARS-Cov2; iPSC disease modelling

Online: 17 April 2023 (03:33:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0345.v1

Regulation of Primary Cilia Length by O-GlcNAc during Neuronal Development in Human Neuron Model

Jie L. Tian, Chia-Wei Huang, Farzad Eslami, Michael Philip Mannino, Rebecca Lee Mai, Gerald W. Hart

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: O-GlcNAc; primary cilia; neuronal development; cortical neurons; human induced-pluripotent stem cells

Online: 14 April 2023 (04:03:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0241.v1

Jak2 and Jaw Muscles Are Required for Mouth Development

Amanda J. G. Dickinson

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: buccopharyngeal membrane; choanal atresia; mouth; Xenopus laevis

Online: 12 April 2023 (04:47:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202301.0150.v2

Comparison of Culture Media for in-vitro Expansion of Oral Epithelial Keratinocytes: Implications for Testing E-liquid Flavors

Giancarlo Cuadra, Abrar Shamim, Raivat Shah, Joey Morgan, Dominic L Palazzolo

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: oral; mucosa; mucins; tight junctions; wound-healing; E-liquids; cytotoxicity; viability; confluency.

Online: 3 April 2023 (03:33:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202304.0003.v1

Knockdown of DOM/Tip60 Complex Subunits Impairs Male Meiosis of Drosophila melanogaster

Yuri Prozzillo, Gaia Fattorini, Diego Ferreri, Manuela Leo, Patrizio Dimitri, Giovanni Messina

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: TIP60; DOMINO; ATPase; Drosophila male meiosis; chromatin remodeling; cell division; epigenetics

Online: 3 April 2023 (02:33:04 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202303.0449.v1

Preliminary Examination of the Principle of Self-Organization in Multicellular Organisms: The Difference in Size Between Yao Ming and Myself

Zheming Cao

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: unstable state cells, growth limit, stem cells, traits

Online: 27 March 2023 (07:50:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0203.v2

Neural Induction, Embryogenesis, and Tumorigenesis

Ying Cao

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: neural induction; embryogenesis; tumorigenesis; neural stemness; tumorigenicity; pluripotency; epithelial-mesenchymal transition; tumor microenvironment

Online: 9 March 2023 (06:57:01 CET)

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Characterization of cancer cells and neural stem cells indicates that tumorigenicity and pluripotency are coupled cell properties determined by neural stemness, and tumorigenesis represents a process of progressive loss of original cell identity and gain of neural stemness. This reminds of a most fundamental process required for the development of the nervous system and body axis during embryogenesis, i.e., embryonic neural induction. Neural induction is that, in response to extracellular signals that are secreted by the Spemann-Mangold organizer in amphibians or the node in mammals and inhibit epidermal fate in ectoderm, the ectodermal cells lose their epidermal fate and assume the neural default fate and consequently, turn into neuroectodermal cells. They further differentiate into the nervous system and also some non-neural cells via interaction with adjacent tissues. Failure in neural induction leads to failure of embryogenesis, and ectopic neural induction due to ectopic organizer or node activity or activation of embryonic neural genes causes a formation of secondary body axis or a conjoined twin. During tumorigenesis, cells progressively lose their original cell identity and gain of neural stemness, and consequently, gain of tumorigenicity and pluripotency, due to various intra-/extracellular insults in cells of a postnatal animal. Tumorigenic cells can be induced to differentiation into normal cells and integrate into normal embryonic development within an embryo. However, they form tumors and cannot integrate into animal tissues/organs in a postnatal animal because of lack of embryonic inducing signals. Combination of studies of developmental and cancer biology indicates that neural induction drives embryogenesis in gastrulating embryos but a similar process drives tumorigenesis in a postnatal animal. Tumorigenicity is the manifestation of aberrant occurrence of pluripotent state in a postnatal animal. Pluripotency and tumorigenicity are both but different manifestations of neural stemness in pre- and postnatal stage, respectively, of animal life. The unique property of neural stemness is derived from the evolutionary advantage of neural genes and the neural-biased state of the last common unicellular ancestors of metazoan. Based on these findings, I discuss about some confusion in cancer research, propose to distinguish the causality and associations and discriminate causal and supporting factors involved in tumorigenesis, and suggest revisiting the focus of cancer research.

Preprint REVIEW | doi:10.20944/preprints202303.0131.v1

Salinity Stress Mitigation by Polyploidy Induction in Soybean (Glycine max L. Merrill)

Phetole Mangena

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Biochemical traits, Colchicine; Glycine max; Morphology; Oryzalin; Polyploidy; Salinity stress; Soybean

Online: 7 March 2023 (09:29:33 CET)

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Preprint REVIEW | doi:10.20944/preprints202302.0448.v1

Aidan McGinnis, Ru-Rong Ji

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: dorsal root ganglia; fatty acids; gliopathy; nerve injury; spinal cord

Online: 27 February 2023 (06:35:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202302.0387.v1

Emerging Biomarkers and Potential Therapeutics of the BCL-2 Protein Family: The Apoptotic and Anti-Apoptotic Context

Shamrat Kumar Paul, Md. Saddam, Mohammad Ahsan Habib, Md. Abrar Fahim, Afsana Mimi, Saiful Islam, Bristi Paul, Md Mostofa Uddin Helal

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: BCL-2 family protein; BCL-2 Domains; Apoptosis; Cancer; Biomarkers; Therapeutic Agents

Online: 22 February 2023 (14:56:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0369.v1

Structure-Function of the Human WAC Protein in GABAergic Neurons: Towards an Understanding of Autosomal Dominant DeSanto-Shinawi Syndrome

Hannah C Rudolph, April M Stafford, Hye-Eun Hwang, Cheol-Hee Kim, Jeremy W Prokop, Daniel Vogt

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cell biology; protein sorting; nuclear translocation; protein domain; WAC

Online: 22 February 2023 (02:37:35 CET)

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Preprint REVIEW | doi:10.20944/preprints202302.0321.v1

Application of Human Stem Cells to Model Genetic Sensorineural Hearing Loss and Meniere Disease

Mar Lamolda, Lidia Frejo, Alvaro Gallego-Martinez, Jose Antonio Lopez-Escamez

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: human induced pluripotent stem cells; inner ear disorders; disease modeling; sensorineural hearing loss; Meniere disease; biomedical applications

Online: 20 February 2023 (06:17:44 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0284.v1

Cbfβ Is a Novel Modulator Against Osteoarthritis by Maintaining Articular Cartilage Homeostasis Through TGF-Ꞵ Signaling

Xiangguo Che, Xian Jin, Narae Park, Hee-June Kim, Hee-Soo Kyung, Hyun-Ju Kim, Jane B. Lian, Janet L. Stein, And Je-Yong Choi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: articular cartilage; Runx1/Cbfβ complex; Osteoarthritis; TGF-β signaling; proteasomal degradation

Online: 16 February 2023 (09:40:30 CET)

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Preprint REVIEW | doi:10.20944/preprints202302.0238.v1

Unravelling the Effects of Berberine on Metabolic Activity, Apoptosis and Proliferation of Transformed Cells

Syed Benazir Alam, Marianna Kulka, Yanwen Wang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: nanoparticles; berberine; metabolism; mitochondria; apoptosis

Online: 14 February 2023 (07:52:01 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0230.v1

Prolonged Piezo1 Activation Induces Cardiac Arrhythmia

Laura Rolland, Angelo Giovanni Torrente, Emmanuel Bourinet, Dounia Maskini, Aurelien Drouard, Philippe Chevalier, Chris Jopling, Adele Faucherre

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cardiac arrhythmia; mechano-electric feedback; Piezo1 channel

Online: 14 February 2023 (03:30:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0161.v1

Impact of Translocator Protein 18 kDa (TSPO)-Deficiency on Mitochondrial Function and Inflammatory State of Human C20 Microglia Cells

Stefanie Bader, Thea Würfel, Tatjana Jahner, Caroline Nothdurfter, Rainer Rupprecht, Vladimir M. Milenkovic, Christian H. Wetzel

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: translocator protein 18 kDa; mitochondria; inflammation; CRISPR/Cas9; respirometry

Online: 9 February 2023 (09:25:55 CET)

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Preprint REVIEW | doi:10.20944/preprints202302.0123.v1

A Glimpse of Ocean of Abundant Discoveries: Two-Way Cross Family Analysis of In-Silico Ranked 2nd Order Unexplored, ETC-1922159 Affected, Synergistic Combinations in CRC Cells

Shriprakash Sinha

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: WNT; NF-κB, Ion Channels; Anthrax toxin recep- tors; Poliovirus receptors; Porcupine inhibitor ETC-1922159; Sensitivity analysis; Colorectal cancer; Unknown biological hypotheses; Combinatorial search space; Support vector ranking

Online: 7 February 2023 (06:16:12 CET)

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Often, in biology, we are faced with the problem of exploring relevant unknown biological hypotheses in the form of myriads of combination of factors that might be affecting the pathway under certain conditions. For example, Brancati et al.1 observe that mutations in poliovirus receptor related protein 4 (PVRL4), encoding cell adhesion molecule nectin-4, causes Ectodermal dysplasia-syndactyly syndrome. Interaction with cad- herins also implies an influence of nectin-4 on Wnt signaling, which plays a relevant role in limb development (Brancati et al.1). However, not much work has been done to explore the relation of Wnts and PVR family. In CRC cells treated with ETC-1922159, both were found up regulated. In a recent unpublished work in Open Science Framework, Sinha2, we had the opportunity to rank these unknown biological hypotheses for both up and down regulated genes at 2nd order level after drug administration. The search engine alloted high nu- merical valued rankings to some combinations of PVR-WNT, thus indicating a possibility of high combinatorial synergy also. The in-silico derived influences can be represented graphically as - PVR w.r.t WNT with PVR <- WNT9A; and WNT w.r.t PVR with WNT-7B/9A <- PVR and WNT4 <- PVRL2; In the light of the recent findings of PVR with IFN (interferon) and the known interactions between IFN and Wnts, there might be a possibilty to explore the bridge of PVR, IFN and WNTs. The 3 fold (PVR - IFN; IFN - WNT; WNT - PVR), 2 way cross family analysis might shed light on the possible combinations that might be of import. Here, we present a 2-way cross family analysis of multiple, such in-silico ranked 2nd order synergistic combinations, after ETC-1922159 treatment of CRC cells. Via this 2-way cross family analysis, we are able to discover through majority voting, the combinations that might of interest to biologists and also derive plausible influences of components of combinations among themselves. Note that these form biological hypotheses which indicate whether a particular combination and the direction of influence within the combination, exist synergistically in CRC cells. Wet lab tests will indicate the veracity of these combinations and if proven true, will lead to further study of mechanism between the components.

Preprint ARTICLE | doi:10.20944/preprints202302.0071.v1

Role of Calcium-Activated Potassium Channels in Proliferation, Migration and Invasion of Human Myeloid Leukemia Cells

Valeria Y. Vasileva, Anastasia V. Sudarikova, Zuleikha M. Khairullina, Vladislav I. Chubinskiy-Nadezhdin

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Calcium-activated potassium channels; SK channels; IK channels; human leukemia cells; cell migration; cancer cell invasion

Online: 3 February 2023 (10:31:24 CET)

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Preprint ARTICLE | doi:10.20944/preprints202302.0002.v1

Osteocyte-Derived CaMKK2 Regulates Osteoclasts and Bone Mass in a Sex-Dependent Manner Through Secreted Calpastatin

Justin N Williams, Mavis Irwin, Yong Li, Anuradha Valiyakambrath, Brett T Mattingly, Sheel Patel, Mizuho Kittaka, Rebecca N Collins, Nicholas Clough, Emma H Doud, Amber L Mosley, Teresita M Bellido, Angela Bruzzaniti, Lilian I. Plotkin, Jonathan C. Trinidad, William R Thompson, Lynda F. Bonewald, Uma Sankar

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Extracellular calpastatin, Ca2+/ calmodulin (CaM)-dependent protein kinase kinase 2, osteocytes, osteoclasts, bone remodeling

Online: 1 February 2023 (01:11:27 CET)

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Preprint REVIEW | doi:10.20944/preprints202301.0555.v1

The Extracellular Matrix: Composition, Function, Remodeling and Its Role in Tumorigenesis

Kevin Dzobo, Collet Dandara

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Extracellular matrix; Tissues; Organs; Development; Tumor progression; Collagens; Fibronectin; Integrins; Metastasis; Matrix metalloproteases; Cell Adhesion; Signaling

Online: 30 January 2023 (11:16:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202301.0269.v1

Comparison Two Methods between Injection Saline and External Fixator in Rat Tail Disc Degeneration

Md Abdul Khaleque, Eun-Yong Choi, Young-Yul Kim, Sang-Eun Park, Hwang-Yong You, Woo-Jin Lee

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Intervertebral disc degeneration; nucleus pulposus; autophagy; apoptosis; external fixator; saline injection

Online: 16 January 2023 (04:44:40 CET)

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Purpose: Intervertebral disc is a leading avascular organ in the body. Hence, it may utilize autophagy and apoptosis to alive in unfavorable condition like stress and mechanical force. But excessive force and stress is a key cause to intervertebral disc degeneration (IVDD). Purpose of this study is to identify, external fixator (EF) and saline injection (SI) may induce autopahgy and apoptosis mediated nucleus pulposus (NP) cells death in rat tail’s disc and which method is better for studying autophagy and apoptosis. Methods: Sixteen 9-week-old male Sprague–Dawley rat tails were treated with 0.9% saline and EF (two-cross kirschner wires) for 6 weeks and 12 weeks as well. Treated discs were dissected for checking out participation of autopahgy and apoptosis to intervertebral disc degradation (IVDD). For identifying them, we performed H&E staining, Masson’s trichrome staining, and Immunohistochemistry (IHC) for LC3, Beclin-1and P62 as well as MMP-2, MMP-3 and TIMP-1.Furthermore, we performed quantitative polymerase chain reaction (qPCR)to check out autophagy related gene expression (beclin-1, LC3 and P62) and apoptosis related gene expression (MMP-2, MMP-3 and TIMP-1) respectively. Results: More insidious nucleus pulposus (NP) cell degeneration was indentified in (EF) group than in (Ctrl) control group. And rate of degradation was elevated with increasing duration of compression, but SI group cannot distinguish the margin of AF and NP cells. Highest along with lateral expression was found for LC3, Beclin-1, and P62 of both groups. Up-regulated and central expression was observed for MMP-2, MMP-3 and TIMP-1 of both groups, although SI group cannot recognize the margin between NP and AF cells. Utmost autophagy related gene expression was identified in EF group than in SI group. Besides, maximum autophagy accumulating materials was found in EF group than in SI group. And it was amplified along with increasing the duration of compression. Highest, apoptotic gene expression was observed in SI group, whereas, EF group showed lowest expression. Conclusion: External fixator (EF) method was better to study autophagy and apoptosis because it enhanced IVDD after compression which is actively linked with autophagy and apoptosis. Degeneration process was conversely proportional along with duration of compression. On contrary, saline injection (SI) could not distinguish AF and NP cell border.

Preprint REVIEW | doi:10.20944/preprints202301.0239.v1

The Nerve Growth Factor Receptor (NGFR/P75^ntr): A Major Player in Alzheimer’s Disease

Francesco Bruno, Paolo Abondio, Alberto Montesanto, Donata Luiselli, Amalia Cecilia Bruni, Raffaele Maletta

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Alzheimer’s disease; Nerve growth factor receptor; NGFR; p75NTR; amyloid-beta; expression; signaling pathways; neuropathology; diagnosis; treatment.

Online: 13 January 2023 (07:37:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202301.0238.v1

Light Sheet Microscopy and 3D Analysis of Human iPSC-derived Embryoid Bodies

Mohammed Yusuf, Jahan Salma, Safana Farooq, Alessia Candeo, Archana Bhartiya, Atiqa Sajid, Marta Szynkiewicz, Ian Robinson, Stan Botchway, El-Nasir Lalani

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: induced pluripotent stem cells; embryoid bodies; light-sheet microscopy; multicolor fluorescence in situ hybridization; chromosomes

Online: 13 January 2023 (07:10:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202301.0159.v1

Altered Mesenchymal Stem Cells Mechanotransduction from Oxidized Collagen. Morphological and Biophysical Observations

Regina Komsa-Penkova, Adelina Yordanova, Pencho Tonchev, Stanimir Kyurkchiev, Svetla Todinova, Velichka Strijkova, Mario Iliev, Borislav Dimitrov, Altankov George

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mesenchymal stem cells; mechanotransduction; collagen; oxidation; YAP/TAZ; focal adhesion

Online: 9 January 2023 (09:53:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202301.0129.v1

Collagen Alpha 1(XI) Is Required for Chondrocyte Differentiation through the AKT/GSK3 β/β-Catenin Signaling Axis in ATDC5 Cells

Jonathon C. Reeck, Julia Thom Oxford

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: chondrogenesis; cartilage; col11a1; collagen α1(XI), AKT; GSK3b; Wnt; ATDC5

Online: 7 January 2023 (13:00:05 CET)

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Preprint REVIEW | doi:10.20944/preprints202301.0099.v1

Ubiquitin-binding Autophagic Receptors in Yeast: Cue5 and beyond

Theresah Nana Ama Mensah, Ankit Shroff, Taras Y. Nazarko

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Atg8; autophagic receptor; Cue5; SAR; selective autophagy; selective autophagy receptor; ubiquitin; ubiquitin-binding domain; ubiquitin-binding protein; ubiquitin-binding receptor

Online: 5 January 2023 (04:33:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202301.0094.v1

An Unexpected Role for GPR143 in Retinal Homeostasis

Dorothy Tung, Nicole R. Congrove, Anna Gabriela Figueroa, Remington M Bliss, Tawanda Zvavamwe, Brian S. McKay

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Retinal pigment epithelium of the eye; RPE; AMD (Age-related Macular Degeneration); GPR143; L-DOPA; Melanin; Ocular albinism; Exosome; Photoreceptor outer segment; Cathepsin D.

Online: 5 January 2023 (02:58:31 CET)

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Age-related macular degeneration is one of the most common causes of blindness, and the incidence exhibits profound racial bias, occurring most frequently in Caucasians. A primary cell type affected in the disease is the retinal pigment epithelium, but the etiology is unclear. The end 10% of the photoreceptor outer segments are shed each day, and the underlying retinal pigment epithelium engulfs, digests, and recycles molecules back to the sensory retina. In previous work, we showed that GPR143 signaling in response to L-DOPA may be effective in the prevention or delay of age-related macular degeneration. In this study, we explore a novel potential effector of GPR143 signaling, that of outer segment uptake and digestion. Using isolated outer segments, labelled with a pH-sensitive marker to fluoresce in lysosomes, we show that GPR143 signaling does not impact outer segment uptake, but does have a significant effect on subsequent degradation. Interestingly, GPR143 signaling did not affect the digestive capacity of the cells, marked by total proteolytic capacity and cathepsin D activity. Rather, our data suggest GPR143 improved endosomal trafficking efficiency of the phagocytosed outer segments to the lysosome. This result is similar to the effect we previously reported for GPR143 on exosome release from the endosomal compartment. Our data illustrate that GPR143 is active in endosomal traffic A single paragraph of about 200 words maximum. For research articles, abstracts should give a pertinent overview of the work. We strongly encourage authors to use the following style of structured abstracts, but without headings: (1) Background: Place the question addressed in a broad context and highlight the purpose of the study; (2) Methods: briefly describe the main methods or treatments applied; (3) Results: summarize the article’s main findings; (4) Conclusions: indicate the main conclusions or interpretations. The abstract should be an objective representation of the article and it must not contain results that are not presented and substantiated in the main text and should not exaggerate the main conclusions.

Preprint BRIEF REPORT | doi:10.20944/preprints202301.0017.v1

Recovery in Transplanted Rat after Stopping the Injected of Immunosuppressant Agent ‘Cyclosporine’

Maryam Naghynajadfard, Ying Li

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: AS rat, xenotransplant, cyclosporine, GFP

Online: 3 January 2023 (08:04:14 CET)

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Preprint REVIEW | doi:10.20944/preprints202212.0520.v1

Ovarian Pregnancy: A Systematic Review and 2 Case Reports

Zukaa Ahmed Almahloul, Bedaya Yasser Amro, Zuhdi Khalid Nagshabandi, Iman Zein Alkiumi, Zeinabsadat Tabatabaei Hakim, Arnaud Wattiez, Muna Abdul Razzaq Tahlak, Philippe R. Koninckx

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: ectopic pregnancy; heterotopic pregnancy; laparoscopy; methotrexate; corpus luteum

Online: 28 December 2022 (01:16:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0391.v1

Genetic and Chemical Controls of Sperm Fate and Spermatocyte Dedifferentiation in Caenorhabditis elegans

Youngyong Park, Matthew Gaddy, Moonjung Hyun, Mariah E Jones, Hafiz M Aslam, Myon Hee Lee

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: PUF-8; MPK-1; sperm fate; dedifferentiation; resveratrol; C. elegans germline

Online: 21 December 2022 (07:08:56 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0254.v1

KDEL Receptor Trafficking to the Plasma Membrane is Regulated by ACBD3 and Rab4-GTP

Chuanting Tan, Yulei Du, Lianhui Zhu, Shuaiyang Jing, Jingkai Gao, Xihua Yue, Yi Qian, Intaek Lee

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: plasma membranes; secretion; ACBD3; KDEL receptor; Rab4; Rab11

Online: 14 December 2022 (09:12:18 CET)

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Preprint REVIEW | doi:10.20944/preprints202212.0212.v1

The Involvement of Krüppel-like Factors in Cardiovascular Diseases

Michelle Giovanna Santoyo-Suarez, Jimena D. Mares-Montemayor, Gerardo R. Padilla-Rivas, Juan L. Delgado-Gallegos, Adriana G. Quiroz-Reyes, Jorge A. Roacho-Perez, Diego F. Benitez-Chao, Lourdes Garza-Ocañas, Gilberto Arevalo-Martinez, Elsa N. Garza-Treviño, Jose Francisco Islas

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cardiovascular diseases; Krüppel -like factors; Transcription factor regulation

Online: 13 December 2022 (01:13:14 CET)

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Preprint REVIEW | doi:10.20944/preprints202212.0153.v1

Crosstalk of Airway Smooth Muscle and Epithelial Cells in Chronic Lung Diseases

Reshed Abohalaka

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: epithelial; smooth muscle; interaction; pulmonary disease; airway; asthma; COPD; bronchial re-modeling

Online: 8 December 2022 (09:46:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202212.0011.v1

MST4: a Potential Oncogene and Therapeutic Target in Breast Cancer

Ritu Arora, Jin-Hwan Kim, Ayechew A. Getu, Anusha Angajala, Yih-Lin Chen, Bin Wang, Andrea G. Kahn, Hong Chen, Latif Reshi, Jianrong Lu, Wenling Zhang, Ming Zhou, Ming Tan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: MST4; EMT; AKT; Apoptosis; Breast cancer

Online: 1 December 2022 (04:46:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202211.0225.v1

Coordination of LMO7 with FAK Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure

Yen-Yi Zhen, Chien-Hsing Wu, Hung-Chun Chen, Eddy Essen Chang, Jia-Jung Lee, Jer-Ming Chang, Wei-Yu Chen, Pei-Yun Tseng, Yue-Fang Wang, Chi-Chih Hung

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: LMO7, secretome, FAK. hypertonicity, osmotic stress, epithelial integrity, epithelial barrier, cortical stress fiber

Online: 14 November 2022 (01:16:06 CET)

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In kidney, epithelial barrier has diverse functions in body fluid and electrolyte homeostasis, and urine production. Maintaining epithelial integrity fundamentally builds up physiological functionality of the renal epithelial barrier (REB). Specially, the REB) states regularly in osmotic dynamics. The osmotic dynamics gives rise of osmotic pressure that is a physical force. Overloading of osmotic pressure can crack epithelial integrity and damage REB. How REB endures the osmotic pressure force yet remains enigmatic. LMO7 (LIM domain only 7) is a protein associated with cell-cell junctional complex and cortical F-actin. LMO7 upregulation was observed in cells cultured in hypertonic condition. In kidney, LMO7 predominantly distributes in epithelial cells in renal tubules. Hypertonic stimulation leads to assembly of LMO7 and F-actin in cortical stress fibers in renal epithelial cells. Hypertonic-isotonic alternation as pressure force pushing plasma membrane inward/outward was set as osmotic disturbance and was applied to test FAK signaling and LMO7 functioning in maintaining junctional integrity. Along with junctional integrity, LMO7 depleted cells resulted in loss of junctional integrity in the epithelial sheet cultured hypertonic medium or hypertonic-isotonic alternation. On the other hand, FAK inhibited by PF-573228 leads to failure in robust cortical F-actin assembly and association of LMO7 with cortical F-actin in epithelial cells responding upon hypertonic stress. Epithelial integrity in context of osmotic stress, LMO7 and FAK signaling both involves in assembling robust cortical F-actin and maintaining junctional integrity. The LMO7 elaborately manages FAK activation in renal epithelial cells, which was evidently demonstrated in NRK-52E cells who have excessive FAK activation and lost epithelial integrity when cells with LMO7 depletion exposed to hypertonic environment. Our data suggests that LOM7 manages FAK activation and is responsible for maintaining REB under osmotic disturbance.

Preprint ARTICLE | doi:10.20944/preprints202211.0074.v1

Oxygen-sensitive TERT Promoter Methylation Regulates Telomerase Activity

Nicholas Robert Forsyth, Fatma Dogan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: telomerase; TERT promoter; DNMT3B; pluripotent stem cells; characterization; epigenetic; meth-ylation; physiological oxygen; DNA methyltransferase

Online: 3 November 2022 (09:16:20 CET)

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Telomere repeats at the ends of human chromosomes protect chromosomes from degradation, and telomerase has a prominent role in telomere maintenance. Telomerase also affects cell proliferation, DNA replication, differentiation, and tumourigenesis. TERT (telomerase reverse transcriptase enzyme) is the catalytic subunit of telomerase and is critical for enzyme activity. TERT promoter mutations and promoter methylation are strongly associated with increased telomerase activation in cancer cells. Notably, TERT and telomerase are downregulated in stem cells during their differentiation. Therefore, the link between differentiation and telomerase provides a valuable tool for studying the epigenetic regulation of TERT enzyme. Oxygen tension affects several cellular behaviours including proliferation, metabolic activity, stemness, and differentiation. The role of oxygen tension in driving promoter modifications of the TERT gene in embryonic stem cells (ESCs) is poorly understood either in vitro or in vivo. We adopted a monolayer ESCs differentiation model to explore the role of low, physiological, oxygen (physoxia) in the epigenetic regulation of telomerase and associated genes, including TERT, DNMTs, and HDACs. Cells were cultured in either air, a 2% O2 incubator, or a 2% O2 oxygen workstation to provide a fully defined 2% O2 environment. Pre-gassed media (pre-conditioned to 2% O2 in a HypoxyCool unit) was used in all 2% O2 experimentation. As anticipated, physoxia culture increased the proliferation rate and stemness of ESCs and a slower onset of differentiation in physoxia was evident. Further, downregulated TERT expression was correlated to reduced telomerase activity during differentiation. TERT expression and telomerase activity remained significantly elevated in physoxia during differentiation. A substantial increase in TERT promoter methylation levels was noted during differentiation. Chemical inhibition of DNMT3B reduced TERT promoter methylation and was associated with increased TERT gene and telomerase activity during differentiation. DNMT3B CHiP demonstrated that downregulated TERT expression and increased proximal promoter methylation were associated with DNMT3B binding to the promoter. In conclusion, we have demonstrated that DNMT3B can directly bind TERT promoter, change its methylation levels, and contribute to regulation of telomerase activity.

Preprint ARTICLE | doi:10.20944/preprints202210.0453.v1

Impact of Metabolic Stress on BeWo Syncytiotrophoblast Function

Lisa Rogers, Marissa Huggins, Ryan Doster, Joel Omage, Alison Eastman, David M. Aronoff

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Gestational diabetes; obesity; placenta; syncytiotrophoblast; matrix metalloprotease

Online: 28 October 2022 (10:04:45 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202210.0435.v1

The Flavonoid Rich Black Currant (Ribes nigrum) Gemmotherapy Extract Prevents Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study

Tímea Téglás, Emőke Mihok, Zoltán Cziáky, Neli-Kinga Oláh, Csaba Nyakas, Endre Máthé

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Ribes nigrum; Gemmotherapy; Neuroinflammation

Online: 27 October 2022 (11:17:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0300.v1

Spatio-Temporal and Cell Type-Specific Expression of Nrf2 Following an Acute Epileptic Seizure in Rats

Sereen Sandouka, Aseel Saadi, Rhoda Olowe, Prince Kumar Singh, Tawfeeq Shekh-Ahmad

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Nrf2; Oxidative stress; Antioxidants; Pentylenetetrazol; Epilepsy; Seizure

Online: 20 October 2022 (08:29:58 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0145.v1

Oxidative Stress-Induced Overactivation of Frog Eggs Triggers Calcium-Dependent Non-Apoptotic Cell Death

Alexander A. Tokmakov, Yudai Morichika, Ryuga Teranishi, Ken-Ichi Sato

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: oxidative stress; Xenopus laevis; eggs; overactivation; cell death

Online: 11 October 2022 (07:08:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202210.0046.v1

Phenotypic Plasticity during the Dissemination of Circulating Tumour Cell Clusters: A Model Involving TGFβ1-Mediated Cluster Dissociation, Adherence and Single-Cell Extravasation

Jorian D. Hapeman, Caroline S. Carneiro, Aurora M. Nedelcu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cancer; circulating tumour cell clusters; metastasis; TGF-β1; extravasation; phenotypic plasticity

Online: 5 October 2022 (16:35:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0476.v1

Degree Days as a Method to Estimate the Optimum Harvest Date of ‘Conference’ Pears

Grzegorz P. Łysiak

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Sum of active temperatures; SAT; CA storage; physiological disorders; TSS; length of growth; harvest date estimation

Online: 30 September 2022 (07:03:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0285.v1

The Chick Embryo Xenograft Model for Malignant Pleural Mesothelioma: A Cost and Time Efficient 3Rs Model for Target and Compound Evaluation

Sarah E Barnett, Anne Herrmann, Elisabeth Gash, Harish Poptani, Joseph J Sacco, Judy M Coulson

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mesothelioma; chick embryo; CAM; xenograft; bioluminescence; fluorescence; histology; MRI; preclinical; 3Rs

Online: 20 September 2022 (02:48:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0160.v2

NIH SenNet Consortium: Mapping Senescent Cells in the Human Body to Understand Health and Disease

Patty J Lee, Philip Blood, Katy Börner, Judith Campisi, Feng Chen, Heike Daldrup-Link, Phil De Jager, Li Ding, Francesca E. Duncan, Oliver Eickelberg, Rong Fan, Toren Finkel, Vesna Garovic, Nils Gehlenborg, Carolyn Glass, Ziv Bar-Joseph, Pragati Katiyar, So-Jin Kim, Melanie Königshoff, George Kuchel, Haesung Lee, Jun H. Lee, Jian Ma, Qin Ma, Simon Melov, Kay Metis, Ana L. Mora, Nicolas Musi, Nicola Neretti, João F. Passos, Irfan Rahman, Juan Carlos Rivera-Mulia, Paul Robson, Mauricio Rojas, Ananda L. Roy, Birgit Schilling, Pixu Shi, Jonathan Silverstein, Vidyani Suryadevera, Jichun Xie, Jinhua Wang, An-Kwok Ian Wong, Laura Niedernhofer

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cellular Senescence Network; Normal Aging; Senescence; Senescence-associated secretory phenotype; SenNet

Online: 15 September 2022 (12:52:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0452.v1

Evolutionary View on Lactate-Dependent Mechanisms of Maintaining Cancer Cells Stemness and Reprimitivization

Petr V. Shegay, Anastasia A. Zabolotneva, Olga P. Shatova, Aleksandr V. Shestopalov, Andrei D. Kaprin

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: lactate; lactic acid; glycolysis; carcinogenesis; malignant tumor; evolutionary oncology; Warburg effect

Online: 26 August 2022 (07:13:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0364.v1

Telomerase Inhibitors TMPyP4 and Thymoquinone Decreased Cell Proliferation and Induced Cell Death in NSCLC Cell Line LC‐HK2, Modifying the Pattern of Focal Adhesion

A.M. Bernabe Garnique, P. Rezende‐Teixeira, G.M. Machado‐Santelli

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: TMPyP4 1; Thymoquinone 2; cell viability 3; cell adhesion 4; telomerase

Online: 19 August 2022 (10:46:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0298.v1

Morphological and Quantitative Evidence for the Altered Mesenchymal Stem Cells Remodeling of Collagen in Oxidative Environment – Peculiar Effect of Epigalocatechin Gallate

Regina Komsa-Penkova, Svetoslava Stoycheva, Pencho Tonchev, Galya Stavreva, Svetla Todinova, Galya Georgieva, Adelina Yordanova, Stanimir Kyurkchiev, George Altankov

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Adipose tissue-derived mesenchymal stem cell; collagen type I; EGCG; oxidation; remodeling

Online: 17 August 2022 (04:11:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0242.v1

Mechanisms of Basement Membrane Micro-Perforation During Cancer Cell Invasion Into a 3D Collagen Gel

Shayan S. Nazari, Andrew D. Doyle, Kenneth M. Yamada

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: hydrogel; 3D-culture; Imaging; Cell-matrix; proteases; matrix metalloproteinases; actin polymerization; contractility

Online: 12 August 2022 (12:55:21 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0203.v1

Neural Induction: the General Principle for Embryogenesis and Tumorigenesis

Ying Cao

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: neural induction; embryogenesis; tumorigenesis; conjoined twin; Spemann organizer; node; neural default model; neural stemness; tumorigenicity; pluripotency; epithelial-mesenchymal transition; tumor microenvironment

Online: 10 August 2022 (12:10:36 CEST)

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Some concepts/hypotheses have been proposed to explain the general rules behind the complexity of tumorigenesis. Characterization of the property of cancer cells and neural stem cells indicates that neural stemness underlies tumorigenicity and pluripotency, leading to the proposal that tumorigenesis represents a process of progressive loss of original cell identity and gain of neural stemness. This reminds of a most fundamental process required for the development of the nervous system and body axis during embryogenesis, i.e., embryonic neural induction. The principle of neural induction is that, in response to extracellular signals that are secreted by the Spemann-Mangold organizer in amphibians or the node in mammals and inhibit epidermal fate in ectoderm, the ectodermal cells assume the neural default fate and turn into neuroectodermal cells. These cells further differentiate into the nervous system and also some non-neural cells via interaction with adjacent tissues. Failure in neural induction leads to failure of embryogenesis, and ectopic neural induction due to ectopic organizer or node activity or activation of embryonic neural genes causes a formation of secondary body axis or conjoined twins. A similar principle underlies tumorigenesis. Increasing evidence has demonstrated that the core property of cancer cells is neural stemness. Therefore, cancer cells are cells with the loss of original cell identity and gain of neural stemness, and consequently tumorigenicity and pluripotency, due to various intra-/extracellular insults in postnatal animals. Unlike that pluripotent cells (embryonic pluripotent cells, neural stem cells and cancer cells) can differentiate and integrate into embryonic development, cancer cells are capable of self-renewal and differentiation, but cannot integrate into normal tissues in a 2 postnatal animal, ultimately leading to tumor formation. Neural induction and the unique property of neural stemness provide an inclusive explanation for embryogenesis, conjoined twin formation and tumorigenesis. Based on these findings, I discuss about some confusion in cancer research, e.g., epithelial-mesenchymal transition, and propose to distinguish the causality and associations, and the causal and supporting factors involved in tumorigenesis, and suggest revisiting the focus of cancer research. Integration of evidence from developmental and cancer biology indicates that neural stemness determines tumorigenicity and pluripotency, and neural induction drives embryogenesis in gastrulating embryos but a similar process drives tumorigenesis in a postnatal animal.

Preprint ARTICLE | doi:10.20944/preprints202208.0122.v1

Formation of Three-Dimensional Spheres Enhances the Neurogenic Potential of Stem Cells from Apical Papilla

Mohammed Basabrain, Jialin Zhong, Haiyun Luo, Junqing Liu, Baicheng Yi, Ahmed Zaeneldin, Junhao Koh, Ting Zou, Chengfei Zhang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: neurogenic potential; neurites; SCAPs; sphere; stem cells

Online: 5 August 2022 (09:59:07 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202208.0025.v1

Next-Generation Sequencing Comparative Analysis of DNA Mutations between Blood-derived Extracellular Vesicles and Matched Cancer Tissue in Patients with IV Grade Glioblastoma

Paolo Rosa, Elena De Falco, Luca Pacini, Amedeo Piazza, Paolo Ciracì, Luca Ricciardi, Francesco Fiorentino, Sokol Trungu, Massimo Miscusi, Antonino Raco, Antonella Calogero

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: glioblastoma; extracellular vesicles; Next Generation Sequencing; pathogenic mutations; NF1

Online: 1 August 2022 (15:07:04 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0423.v1

Is “Cellular Senescence” a Misnomer?

David Gems, Carina Kern

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Aging; cellular senescence; fibroblast; osteoarthritis; remodeling-associated secretory phenotype (RASP); remodeling activation

Online: 27 July 2022 (13:38:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0376.v1

Information Theory as an Experimental Tool for Integrating Disparate Biophysical Signaling Modules

Patrick McMillen, Michael Levin

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: information theory; embryogenesis; regeneration; cell biology; morphogenesis; calcium

Online: 25 July 2022 (12:35:28 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0353.v1

Cancer Stem Cells and Competing Endogenous RNAs

Hamid Aria, Mahdieh Azizi, Shima Nazem, Maryam Bahmanyar, Ali Moravej, Mohammad Hosein Pourjafarian, Babak Pezeshki, Alireza Tavassoli, Mohammad Kazem Vakil, Yaser Mansoori

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cancer stem cells; competing endogenous RNAs; ceRNA; lncRNA; micro-RNA; miRNA

Online: 25 July 2022 (06:18:30 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202207.0106.v2

MRTF May Be the Missing Link in a Multiscale Mechanobiology Approach toward Macrophage Dysfunction in Space

Rocky An

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mechanobiology; microgravity; macrophage; multiscale; MRTF; radiation

Online: 18 July 2022 (10:59:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0167.v1

Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

Alexandra Calle, Miguel Ángel Ramírez

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: European mink; ex situ conservation; biobank; mesenchymal stem/stromal cells

Online: 12 July 2022 (04:04:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0160.v1

NIH SenNet Consortium: Mapping Senescent Cells in the Human Body to Understand Health and Disease

Patty Lee, Philip Blood, Katy Börner, Judith Campisi, Feng Chen, Heike Daldrup-Link, Phil De Jager, Li Ding, Francesca E. Duncan, Oliver Eickelberg, Rong Fan, Toren Finkel, Vesna Garovic, Nils Gehlenborg, Carolyn Glass, Ziv Bar-Joseph, Pragati Katiyar, So-Jin Kim, Melanie Königshoff, George Kuchel, Haesung Lee, Jun H. Lee, Jian Ma, Qin Ma, Simon Melov, Kay Metis, Ana L. Mora, Nicolas Musi, Nicola Neretti, João F. Passos, Irfan Rahman, Juan Carlos Rivera-Mulia, Paul Robson, Mauricio Rojas, Ananda L. Roy, Birgit Schilling, Pixu Shi, Jonathan Silverstein, Vidyani Suryadevera, Jichun Xie, Jinhua Wang, An-Kwok Ian Wong, Laura Niedernhofer

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cellular Senescence Network; Normal Aging; Senescence; Senescence-associated secretory phenotype; SenNet

Online: 11 July 2022 (12:12:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0152.v1

Deregulation of Mitochondrial Calcium Handling Due to Presenilin Loss Disrupts Redox Homeostasis and Promotes Neuronal Dysfunction

Kerry C. Ryan, Jocelyn T. Laboy, Kenneth R. Norman

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Alzheimer’s disease; oxidative stress; presenilin; mitochondria; calcium; neuronal dysfunction; Nrf2

Online: 11 July 2022 (08:03:08 CEST)

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Preprint HYPOTHESIS | doi:10.20944/preprints202207.0106.v1

MRTF May Be the Missing Link in a Multiscale Mechanobiology Approach toward Macrophage Dysfunction in Space

Rocky An

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mechanobiology; microgravity; macrophage; multiscale; MRTF; radiation

Online: 7 July 2022 (04:08:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0234.v1

Alcohol Dependence Induces CRF Sensitivity in Female Central Amygdala GABA Synapses

Larry Rodriguez, Dean Kirson, Sarah A. Wolfe, Reesha R. Patel, Florence P. Varodayan, Angela E. Snyder, Pauravi J. Gandhi, Sophia Khom, Roman Vlkolinksy, Michal Bajo, Marisa Roberto

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: corticotropin releasing factor (CRF); patch-clamp electrophysiology; sex difference; alcohol use disorder (AUD); Gamma-Aminobutyric Acid (GABA); central amygdala (CeA); spontaneous inhibitory post synaptic currents (sIPSCs)

Online: 16 June 2022 (08:41:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0165.v1

A New Transgenic Tool to Study the Ret Signaling Pathway in the Enteric Nervous System

Ashoka Bandla, Ellie Melancon, Charlotte Taylor, Judith S Eisen, Julia Ganz

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Hirschsprung Disease; neuronal development; enteric neuron; enteric progenitor cell; zebrafish; ENS neuropathies

Online: 13 June 2022 (03:48:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0095.v1

Optimization of Mechanosensitive Cross-talk between Matrix Stiffness and Protein Density: Independent Matrix Properties Regulate Spreading Dynamics of Myocytes

Judith Brock, Julia Erhardt, Stephan A. Eisler, Marcel Hörning

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mechanosensitivity; proliferation; myocyte; actincytoskeleton; extracellular matrix; L-DOPA

Online: 7 June 2022 (08:05:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0256.v1

Enhancement of Neuroglial Extracellular Matrix Formation and Physiological Activity of Dopaminergic Neural Cocultures by Macromolecular Crowding

Srikanya Kundu, Andy N. Vo, Caroline Strong, Olive Jung, Emily Lee, Min Jae Song, Molly E. Boutin, Michael Raghunath, Marc Ferrer

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Extracellular matrices; Macromolecular crowding; human iPSC derived astrocytes; human iPSC derived dopaminergic neurons; drug testing

Online: 19 May 2022 (08:50:28 CEST)

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Preprint REVIEW | doi:10.20944/preprints202205.0217.v1

Longevity, Aging and Cancer: Thermodynamics and Complexity

J.M. Nieto-Villar, R. Mansilla

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: longevity; aging; cancer; complex systems; non-equilibrium thermodynamics; biological phase transition; ferroptosis

Online: 17 May 2022 (03:16:18 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0089.v1

HSPA1B is the Dominant Partner in the HSPA1A/HSPA1B Mediated Proteotoxic Stress Response in Placenta-Derived Stem Cells

Bothina Mohammed Alharbi, Aisha Bugshan, Azhaar Almozel, Reem Alenzi, Abderrezak Bouchama, Tanvir Khatlani, Sameer Mohammad, Shuja Shafi Malik

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: placenta-derived stem cells; placenta; stem cells; proteostasis; heat-shock; chaperones; HSPA1B; HSPA1A

Online: 7 May 2022 (03:37:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0033.v1

Clpp-Deletion Causes Azoospermia, with Meiosis-I Delay and Insufficient Biosynthesis of Spermatid Factors, Due to Mitochondrial Dysfunction with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2

Suzana Gispert, Jana Key, Aneesha Kohli, Sylvia Torres-Odio, Gabriele Koepf, Shady Amr, Marina Reichlmeir, Patrick N. Harter, A. Phillip West, Christian Münch, Georg Auburger

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: meiosis-I; zygotene-pachytene; homologous recombination; H3K9ac; acetyl-tubulin; Twinkle helicase; RMND1; tRNA /rRNA processing; cGAS-STING signaling

Online: 5 May 2022 (10:41:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0307.v1

Novel Cell Receptor System of Eukaryotes Formed by Previously Unknown Nucleic Acid-Based Receptors

Victor Tetz, George Tetz

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: receptors; extracellular DNA; cell-surface bound DNA; cell-surface bound RNA

Online: 29 April 2022 (15:04:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0150.v1

Genetic interaction of Thm2 and Thm1 Shapes Postnatal Craniofacial Bone

Erin Bumann, Portia Hahn Leat, Henry Wang, Brittany Hufft-Martinez, Wei Wang, Pamela Tran

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Ttc21a; Ttc21b; mouse knock-out; jaw; facial bone; viscerocranium; Hh signaling

Online: 16 April 2022 (03:36:54 CEST)

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Preprint REVIEW | doi:10.20944/preprints202204.0084.v1

How to Heal the Gut’s Brain: Regeneration of the Enteric Nervous System

Helen Rueckert, Julia Ganz

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: enteric progenitor cell; zebrafish; inflammation; Hirschsprung Disease; neural crest cell; ENS neuropathies

Online: 9 April 2022 (01:49:53 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202204.0070.v1

Single Ion Channel Recording in 3D Culture of Stem Cells Using Patch-Clamp Technique

Vladislav I. Chubinskiy-Nadezhdin, Anastasia V. Sudarikova, Mariia A. Shorokhova, Valeria Y. Vasileva, Zuleikha M. Khairullina, Yuri A. Negulyaev

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: 3D cell culture; patch-clamp; spheroids; single-channel recording; ion channels; mesenchymal stem cells

Online: 8 April 2022 (03:29:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0143.v1

Physiological Oxygen Causes the Release of Volatile Organic Compounds from Human Pluripotent Stem Cells with Possible Roles in Maintaining Self-Renewal and Pluripotency

Sara Barreto, Mohammed A. Al-Zubaidi, Tina P. Dale, Amy J. Worrall, Zoher Kapacee, Susan J. Kimber, Josep Sulé-Suso, Nicholas R. Forsyth, Abigail V. Rutter

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: human pluripotent stem cells; human embryonic stem cells; human induced pluripotent stem cells; cellular metabolism; oxygen; self-renewal; pluripotency; differentiation; volatile organic compounds; Selected ion flow tube-mass spectrometry

Online: 10 March 2022 (10:23:46 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0133.v1

A New Technique to Repair Large Bone Defects

Takaaki Tanaka, Hirokazu Komaki, Naoya Inagaki, Shoshi Akiyama, Mitsuru Saito

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: bone formation; BMP-2; FGF-2; beta-TCP; osteoblast-osteoclast communication

Online: 9 March 2022 (10:58:33 CET)

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Biology and Life Sciences