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Preprint REVIEW | doi:10.20944/preprints202404.1199.v1

Clinical Correlation of Transcription Factor SOX3 in Cancer: Unveiling Its Role in Tumorigenesis

Helen Lima Del Puerto, Ana Paula G.S. Miranda, Dinah Qutob, Enio Ferreira, Felipe H.S. Silva, Bruna M. Lima, Barbara A. Carvalho, Bruna R. Souza, Eduardo Gutseit, Diego C. Castro, Emanuele T. Pozzolini, Nayara O. Duarte, Thacyana B.G. Lopes, Daiana Y.O. Taborda, Stella M. Quirino, Ahmed Elgerbi, John S. Choy, Adam Underwood

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: SOX3; Cancer; Apoptosis; EMT; Invasion; Migration; Cell cycle; Proliferation; Prognosis; Therapeutics

Online: 18 April 2024 (13:42:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.1106.v1

α‐Catenin and Piezo Mediate Cell Mechanical Communication through Cell Adhesions

Qingyu Zhang, Bing Bu, Yiming Zhu, Jingjing Li, Mingzhi Luo, Linhong Deng, Mingxing Ouyang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cell mechanical communication; α-Catenin, Piezo; directional migration; focal adhesion; mechanotransduction

Online: 17 April 2024 (11:24:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202402.1729.v2

Epithelial-Mesenchymal Transition: A Groundless Skyscraper

Ying Cao

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: epithelial– mesenchymal transition, mesenchymal-epithelial transition, endothelial-mesenchymal transition, epithelial state, mesenchymal state, controversy, developmental biology, fibrosis, cancer, neural stemness, tumorigenicity, pluripotency

Online: 16 April 2024 (15:50:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0749.v1

LSD2 is an Epigenetic Player in Multiple Types of Cancer and Beyond

Hyun-Min Kim, Zifei Liu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: LSD2; KDM1B; AOF1; LSD1; KDM1A; histone demethylase; cancer; DNA repair

Online: 11 April 2024 (07:57:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0711.v1

Unlocking glioblastoma vulnerabilities with CRISPR-based genetic screening

Yitong Fang, Xing Li, Ruilin Tian

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: glioblastoma; CRISPR-based genetic screening; therapy resistance; genetic determinants; therapeutic targets

Online: 10 April 2024 (08:14:36 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0654.v1

Tumor Evolution Is Directed by Cellular Responses to Survival Challenges

Helen Zhang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: tumor evolution; tumor heterogeneity; oncogene addiction; target therapy

Online: 9 April 2024 (10:33:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202404.0621.v1

Targeting the Hippo Pathway in Melanoma

Urszula Kazimierczak, Anna Przybyła, Marianna Smielowska, Tomasz Kolenda, Andrzej Mackiewicz

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Hippo signaling; melanoma; therapy

Online: 9 April 2024 (08:52:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202404.0173.v1

Cell Recruitment and the Origin of Anterior-Posterior Asymmetry in the Drosophila Wing

Rosalio Reyes, Rafael Rodriguez-Muñoz, Marcos Nahmad

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Drosophila wing; Anterior-Posterior; cell recruitment; asymmetry; morphometrics

Online: 2 April 2024 (16:19:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.0828.v2

Illuminating Intercellular Autophagy: A Comprehensive Review of Cell Non-Autonomous Autophagy

Karan Selarka, Bhupendra V. Shravage

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: autophagy; cell non-autonomous; disease; regulation; cell-autonomous; intercellular/organ

Online: 2 April 2024 (09:55:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202403.1664.v1

Nitric Oxide in the Mechanisms of Cell Death and Cytoprotection

Igor Belenichev, Olena Popazova, Nina Bukhtiyarova, Dmytro Savchenko, Valentyn Oksenych, Oleksandr Kamyshnyi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Nitric oxide; NO; inducible nitric oxide synthases; iNOS; endothelial nitric oxide synthases; eNOS; heat shock proteins; HSP

Online: 27 March 2024 (10:05:35 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.1586.v1

The Role of Regulatory T Cells and Their Therapeutic Potential in Hypertensive Disease of Pregnancy; A Literature Review

Kyle Headen, Vaidile Jakaite, Vita Andreja Mesaric, Cristiano Scottà, Giovanna Lombardi, Kypros H Nicolaides, Panicos Shangaris

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Tregs; preeclampsia; hypertensive disorders; immune tolerance; semi allogenic fetus

Online: 26 March 2024 (11:34:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1293.v1

Algebraic Nexus of Fibonacci Forms and 2-Simplex Topology in Multicellular Morphogenesis

William E. Butler Hoyos, Héctor Andrade Loarca, Kristopher Kahle, Ziv Williams, Elizabeth G. Lamb, Julio Alcantara, T. Bernard Kinane, Luis Turcio

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mitosis; Fibonacci; Golden Ratio; epigenomics; simplex topology; cell adhesion; organogenesis; morphogenesis

Online: 22 March 2024 (11:34:34 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.1200.v1

On Mast Cells as Protectors of Life, Reproduction, and Progeny

Klas Carl Norrby

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Mast cells; Sentinel cell; Health; Ovulation; Pregnancy; Inflammation; Wound healing; Cell prolif-eration; Angiogenesis; bFGF; VEGF-A; Tissue remodeling; Histamine; Serotonin; Heparin; Proteases; IL-1-alpha; IL-8; TNF-alpha; Nitric oxide; Survival; Progeny

Online: 20 March 2024 (09:50:02 CET)

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Preprint BRIEF REPORT | doi:10.20944/preprints202403.1184.v1

Effect of M694V mutation in the MEFV gene, associated with Familial Mediterranean fever, on the morphology of iPSC-derived macrophages

Elena V. Grigor’eva, Lana V. Karapetyan, Anastasia A. Malakhova, Sergey P. Medvedev, Julia M. Minina, Varduhi H. Hayrapetyan, Valentina S. Vardanyan, Suren M. Zakian, Arsen Arakelyan, Roksana Zakharyan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Familial Mediterranean fever; macrophages; patient-specific induced pluripotent stem cells; differentiation; MEFV gene

Online: 20 March 2024 (08:24:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1072.v1

Cytokine Receptor Like Factor 1 (CRLF1) and its Role in Osteochondral Repair

Fenglin Zhang, Andrew J. Clair, John F. Dankert, You Jin Lee, Kirk A. Campbell, Thorsten Kirsch

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: osteochondral defect repair; CRLF1; Mesenchymal stem cells; chondrogenic differentiation; chondrocytes

Online: 19 March 2024 (05:24:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.1042.v1

The Role of Vimentin in Human Corneal Fibroblast Spreading and Myofibroblast Transformation

Miguel Miron-Mendoza, Kara Poole, Sophie DiCesare, Emi Nakahara, Meet P Bhatt, John D Hulleman, W. Matthew Petroll

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cornea; vimentin; cytoskeleton; fibroblasts; myofibroblasts; wound healing; TGFb; PDGF

Online: 18 March 2024 (16:11:17 CET)

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Preprint REVIEW | doi:10.20944/preprints202403.0828.v1

Illuminating Intercellular Autophagy: A Comprehensive Review of Cell Non-Autonomous Autophagy

Karan Selarka, Bhupendra V. Shravage

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: autophagy; cell non-autonomous; disease; regulation; cell-autonomous; intercellular/organ

Online: 15 March 2024 (14:40:02 CET)

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Preprint ARTICLE | doi:10.20944/preprints202309.2006.v2

Equine Endothelial Cells Show Pro-angiogenic Behaviours in Response to FGF2 but Not VEGF-A

Elizabeth JT Finding, Ashton Faulkner, Caroline PD Wheeler-Jones

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: equine; endothelial; angiogenesis; comparative

Online: 14 March 2024 (11:12:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0693.v1

miR-1 as a Key Epigenetic Regulator in Early Differentiation of Cardiac Sinoatrial Region

Carlos García-Padilla, Estefanía Lozano-Velasco, Virginio García-López, Amelia Aránega, Diego Franco, Virginio García-Martínez, Carmen López-Sánchez

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: miR-1; epigenetic regulator; cardiac sinoatrial region; molecular mechanisms; HDAC4; Calmodulin; pErk2/MAPK1; Mef2c activity; retinoic acid signaling; cardiac development

Online: 12 March 2024 (10:22:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0151.v1

Functional Analysis of Forkhead Transcription Factor Fd59a in Spermatogenesis of Drosophila melanogaster

Ting Tang, Mengyuan Pei, Yanhong Xiao, Yingshan Deng, Yuzhen Lu, Xiao-Qiang Yu, Liang Wen, Qihao Hu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Spermatogenesis; Forkhead; FoxD; Testis; Apoptosis

Online: 5 March 2024 (05:01:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0073.v1

Intraphagosomal Free Ca2+Changes during Phagocytosis

Maurice B. Hallett, Sharon Dewitt, Iraj Laffafian, Kimberly J Lewis, Joanna Green

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: intra-phagosomal Ca2+, phagocytosis; Ca2+channels; phagosome; neutrophil

Online: 4 March 2024 (10:32:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202403.0068.v1

Identification, Expression and Evolutional Analysis of Two cyp19-Like Genes in Amphioxus

Yajun Wang, Jingyuan Lin, Wenjin Li, Guangdong Ji, Zhenhui Liu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Amphioxus; Invertebrates; Sex differentiation; Cyp19

Online: 1 March 2024 (18:54:16 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1729.v1

The Unknown Epithelial and Mesenchymal States Cannot Support Epithelial-Mesenchymal Transition as a Meaningful Concept

Ying Cao

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: epithelial– mesenchymal transition; mesenchymal-epithelial transition; endothelial-mesenchymal transition; epithelial state; mesenchymal state; history; controversy; developmental biology; fibrosis; cancer; neural stemness; tumorigenicity; pluripotency

Online: 29 February 2024 (10:50:14 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.1653.v1

The Evolutionary Foundations of Animal Transcriptional Regulatory Mechanisms

Maxwell Coyle, Nicole King

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: transcription, transcription factors, animal origins, enhancers, choanoflagellates, Myc:Max, RFX, Brachyury, chromatin, distal regulation

Online: 28 February 2024 (15:59:48 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1581.v1

Identification and Characterization of the Gene responsible for 2 the O3 Mating Type Substance in Paramecium caudatum

Yuta Chiba, Yasuhiro Takenaka, Nobuyuki Haga

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: mating-type substance; kinase-C domain; EF hand motif; Phylogenic tree; RNAi; silent mutations; microinjection; Paramecium caudatum

Online: 28 February 2024 (00:09:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1549.v1

Cell Cycle Kinetics and Sister Chromatid Exchange in Mosaic Turner Syndrome

Miriam Beatriz Beatriz Goulart, Eduardo Vieira Neto, Daniela R. Ney Gracia, Marília Martins Guimarães, Isaías Soares De Paiva, Karina De Ferran, Nathalia Correia Krause Dos Santos, Luciana Santos Barbosa, Amanda F. De Figueiredo, Maria Cecília Menks Ribeiro, Márcia Gonçalves Ribeiro

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Turner syndrome; chromosomal mosaicism; cell lineages; proliferation index; sister chromatid exchange; chromosomal instability

Online: 27 February 2024 (12:34:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1517.v1

Visualization of dimeric protein complex association on specific enhancers in salivary gland nuclei of the Drosophila larva

Solène Vanderperre, Samir Merabet

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: protein-protein interaction, BiFC, ANCHOR, ParB-INT, Hox

Online: 27 February 2024 (09:20:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0993.v1

In Vivo-Matured Oocyte Resists Post-ovulatory Aging through the Post-transcriptional Regulation in Pigs

Cheng-Lin Zhan, Dongjie Zhou, Ming-Hong Sun, Wen-Jie Jiang, Song-Hee Lee, Xiao-Han Li, Qin-Yue Lu, Ji-Dam Kim, Gyu-Hyun Lee, Jae-Min Sim, Hak-Jae Chung, Eun-Seok Cho, Soo-Jin Sa, Xiang-Shun Cui

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: post-ovulatory aging; oocyte quality; mitochondrial function; post-transcriptional regulation

Online: 19 February 2024 (16:01:18 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.1027.v1

TRIM2 Selectively Regulates Inflammation-Driven Pathological Angiogenesis without Affecting Physiological Hypoxia-Mediated Angiogenesis

Nathan K.P. Wong, Emma L. Solly, Richard Le, Victoria A. Nankivell, Jocelyne Mulangala, Peter J. Psaltis, Stephen J. Nicholls, Martin K.C. Ng, Christina A. Bursill, Joanne T.M. Tan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Inflammation; Ischemia; Neovascularization; HIF-1α ; eNOS

Online: 19 February 2024 (08:33:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0907.v1

PI 3-Kinase and the Histone Methyl-Transferase KMT2D Collaborate to Induce Arp2/3-Dependent Migration of Mammary Epithelial Cells

Karina D Rysenkova, Julia Gaboriaud, Artem I Fokin, Raphaëlle Toubiana, Alexandre Bense, Camil Mirdass, Mélissa Jin, Minh Chau N Ho, Elizabeth Glading, Sophie Vacher, Laura Courtois, Ivan Bièche, Alexis M Gautreau

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: KMT2D; MLL2; MLL4; PIK3CA; Arp2/3

Online: 16 February 2024 (10:12:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0766.v1

The Effect of Stress Hormone Signaling on Epithelial Wound Healing

Hannah Smid, Juhi Shah, Riadh Cheddadi, Rotimi Kukoyi, Brandon Horn, Colin Martin

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Wound healing; Necrotizing enterocolitis; Stress signaling; Intestinal Injury

Online: 14 February 2024 (09:35:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0665.v1

Spatial Computational Hepatic Molecular Biomarker Reveals LSECs Role in Mid Lobular Liver Zonation Fibrosis in DILI and NASH Liver Injury

Munish Puri

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: NASH; DILI; LSEC; DGE; liver zonation marker; bridging fibrosis; spatial transcriptomics; liver injury; computational biomarker

Online: 12 February 2024 (09:44:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0568.v1

The Sarcoplasmic/Endoplasmic Reticulum Ca2+-ATPase (SERCA) Activator CDN1163 Exerts Complex Time-Dependent and SERCA Isoform-Specific Effects on T Lymphocyte Ca2+ Store Functions

MD Nasim Uddin, David W. Thomas

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Ca2+ Stores; SERCA Regulation; ER Ca2+ Pools; T Cell Activation

Online: 9 February 2024 (10:06:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202402.0403.v1

CXCL1/CXCR2 and CCL5/CCR1/CCR5 Promote Radiation-Induced Reprogramming of Breast Non-cancer Stem Cells into Cancer Stem Cells and Predict Patient Clinical Outcome

Justine Bailleul, Mathilde Brulé, Nadège Bidan, Marie Denoulet, Raphaelle Mouttet Audouard, Sophie Cousin, Emmanuel Bouchaert, Jerome Benoit, Robert-Alain Toillon, Pascal Finetti, Daniel Birnbaum, François Bertucci, Eric Adriaenssens, Xuefen Le Bourhis, Chann Lagadec

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cancer Stem Cells; Breast Cancer; Reprogramming; Chemokines; Radiotherapy

Online: 7 February 2024 (06:27:18 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0346.v1

Subepithelial Stromal Cells: Their Roles and Interactions with Intestinal Epithelial Cells during Gut Mucosal Homeostasis and Regeneration

Hammed Ayansola, Edith J Mayorga, Younggeon Jin

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Intestinal stem cell niche; mesenchymal stromal cells; sub-epithelial gradient factors; epithelial-mesenchymal interactions

Online: 6 February 2024 (09:40:19 CET)

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Preprint REVIEW | doi:10.20944/preprints202402.0083.v1

Hedgehog on the Move: Glypican-Regulated Transport and Gradient Formation in Drosophila

Carlos Jiménez-Jiménez, Kay Grobe, Isabel Guerrero

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: glypicans; heparan sulfate proteoglycans; Dally; Dally-Like; Hedgehog.

Online: 1 February 2024 (15:28:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1821.v1

Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile

Jeniffer Acosta-Cárdenas, Luis Felipe Jiménez-García, Sarai de Jesús Cruz-Gómez, Ana Paulina Mendoza-von der Borch, María de Lourdes Segura-Valdez

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cell nucleus; nuclear speckles; reptile; splicing factors; SR proteins; ribonucleoproteins

Online: 25 January 2024 (14:08:37 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1738.v1

Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B Cells through Tunnelling Nanotubes

Henriett Halász, Viktória Tárnai, János Matkó, Miklós Nyitrai, Edina Szabó-Meleg

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: actin; microtubule; mitochondria; transport; membrane nanotube; motor proteins

Online: 24 January 2024 (11:04:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1726.v1

Spontaneous Overactivation of Xenopus Frog Eggs Triggers Necrotic Cell Death

Alexander A. Tokmakov, Ryuga Teranishi, Ken-Ichi Sato

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Xenopus laevis; eggs; spontaneous activation; mechanical stress; necrosis

Online: 24 January 2024 (07:29:21 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1661.v1

Harnessing NKG2D CAR-T Cell Therapy to Target Solid Tumors: Advancements and Applications

Vitaly Chasov, Albert Rizvanov

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Immunotherapy; cell therapy; CAR-T cells; chimeric antigen receptor; NKG2D

Online: 23 January 2024 (07:43:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1450.v1

A Pharmacological Investigation of the TMEM16A Currents in Murine Skeletal Myogenic Precursor Cells

Marina Sciancalepore, Asja Ragnini, Paola Zacchi, Violetta Borelli, Paola D’Andrea, Paola Lorenzon, Annalisa Bernareggi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: TMEM16A; Piezo1; Ani9; TMEM16inh-A01; Yoda1; skeletal muscle; myogenesis

Online: 19 January 2024 (04:26:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1437.v1

A Keratin 12 Expression-Based Analysis of Stem-Precursor Cells and Differentiation in The Limbal-Corneal Epithelium Using Single-Cell RNA-Seq Data

J. Mario Wolosin

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: scRNA-Seq, cornea, limbus, epithelium, Krt12, stem cells, differentiation, gene expression

Online: 18 January 2024 (15:54:32 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.1118.v1

Lessons from the Thioredoxin-1 Knockout Mouse Models

Tetiana Shcholok, Eftekhar Eftekharpour

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Redox balance, thiol antioxidants, transgenic models, brain, heart, glutaredoxin, thioredoxin reductase

Online: 15 January 2024 (10:39:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.1007.v1

Tumor-Associated Macrophages (TAMs) Polarization by Single-Cell Sequencing Combined with Bulk Data in Hepatocellular Carcinoma Cell

Liusheng Wu, Xinye Qian, Shuang Wang, Shuang Wang, Xiaoqiang Li, Jun Yan

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Hepatocellular carcinoma; Macrophage activation; Single-cell sequencing; Bulk data analysis; ALDH2

Online: 15 January 2024 (02:24:58 CET)

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[Objective] To investigate the expression of ALDH2 in hepatocellular carcinoma and its correlation with macrophage infiltration and with the activation of specific subtypes of macrophages. [Methods] Transcriptome and clinical data from patients with hepatocellular carcinoma were downloaded from The Cancer Genome Atlas (TCGA) and the International Cancer Genome Consortium (ICGC) databases and analyzed and collated using R and Perl software. In the TCGA hepatocellular carcinoma dataset, the Wilcoxon test was used to identify macrophage activation-related gene sets that show differential expression between hepatocellular carcinoma and normal tissues. Single-cell sequencing combined with bulk data was used to analyze macrophage infiltration of hepatocellular carcinoma immune cells and the activation and recruitment of different subtypes of macrophages. Single-factor Cox regression and minimum absolute convergence and selection operator (Lasso) were used to identify survival-related genes and construct prognostic models of HCC. The patients were divided into two groups based on their model scores. The Wilcoxon test was used to identify differentially expressed genes (DEGs) between the two groups, and gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses of the DEGs were performed. Single-sample gene set enrichment analysis (ssGSEA) was performed to explore the possible mechanism underlying the differences in prognosis. The reliability of the model was verified using the ICGC hepatocellular carcinoma dataset. A Kaplan‒Meier curve was constructed and used to analyze the influence of macrophage activation-related gene expression on survival and prognosis in patients with hepatocellular carcinoma. The log-rank test and the independent t test were used to analyze survival time data and to compare immune cell infiltration in the two groups. [Results] In a comparison of hepatocellular carcinoma and paracancerous tissues, the expression of six of 137 macrophage activation-related genes (ALDH2, CCNB2, CYP2C9, CYP3A4, F9 and KLKB1) was found to be significantly correlated with overall survival time (OS) in patients with hepatocellular carcinoma. Based on this finding, a prognosis assessment model for hepatocellular carcinoma was constructed. The OS of the patients in the high-scoring group was significantly shorter than the OS of the patients in the low-scoring group (P<0.05), and the model predicted the prognosis of patients with hepatocellular carcinoma independently of sex, age, tumor grade and stage. The area under the receiver operating characteristic (ROC) curve of the model used to predict 1-, 2-, and 3-year survival was greater than 0.7 in both the test set and the validation set. GO enrichment of DEGs between the high- and low-scoring groups of the model suggested that the DEGs are mainly related to immune function. GSEA suggested that the tumor-infiltrating immune cells in the high-scoring group were macrophages, Th2 cells and Treg cells and showed that the number of tumor-infiltrating immune cells, including NK cells, was lower in the high-scoring group than in the low-scoring group. With respect to immune function, the immune checkpoint of the high-scoring group was higher than that of the low-scoring group. Single-cell sequencing data indicated the recruitment of B cells, M0 cells, M1 cells and M2 cells to the hepatocellular carcinoma microenvironment and suggested that ALDH2 regulates related signaling pathways in a way that promotes the differentiation of macrophages into M2-type macrophages. [Conclusion] M2-type macrophages are the dominant type of macrophages associated with immune infiltration in hepatocellular carcinoma. The macrophage-associated activation gene ALDH2 activates the macrophage polarization signaling pathway and negatively regulates the differentiation of macrophages into M2-type macrophages.

Preprint ARTICLE | doi:10.20944/preprints202401.0999.v1

miR-223, but Not miR-195, Modulates Myocardial-Induced Proepicardial Cell Migration

Estefanía Lozano-Velasco, Amelia Aranega, Diego Franco

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: microRNAs; proepicardium; cell migration

Online: 12 January 2024 (09:09:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0637.v1

Triple Silencing of HSP27, cFLIP, and CLU Genes Promotes Sensitivity of Doxazosin-Induced Apoptosis in PC-3 Prostate Cancer Cells

Jeong Man Cho, Sojung Sun, Eunji Im, Hyunwon Yang, Tag Keun Yoo

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Apoptosis; CLU; cFLIP; Doxazosin; HSP27; Prostate cancer

Online: 9 January 2024 (03:07:49 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202401.0590.v1

A Modified Tridecapeptide Probe for Imaging Cell Junction

Li Jingrui, Yuhan Wu, Chunyu Liu, Shu Zhang, Xin Su, Songbo Xie, Fengtang Yang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cell junction; Cx43-derived peptide; Cell-penetrating peptide; ZO-1

Online: 8 January 2024 (09:09:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202401.0520.v1

Autophagy of Glycogen Granules is a Non-Selective Process in Nitrogen Starved Komagataella phaffii

Nimna V. Wijewantha, Ravinder Kumar, Taras Y. Nazarko

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: autophagy; Glg1; glycogen; glycogen granules; glycophagy; Komagataella phaffii; non-selective autophagy; Pichia pastoris; selective autophagy; yeast

Online: 8 January 2024 (06:39:39 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202401.0111.v2

Repair and Misrepair of Telomeric DNA in Dynamic Interactions with PML Nuclear Bodies and Lamin B1—A Gear-Wheel Nuclear Traffic.

Kristine Salmina, Fēlikss Rūmnieks, Dace Pjanova, Jekaterina Erenpreisa

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cancer; cellular senescence; telomere attrition; alternative telomere lengthening; PML nuclear bodies; lamin B1; ALU; surface heterochromatin; nuclear rotation; chromothripsis

Online: 5 January 2024 (10:06:39 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0923.v2

Brain Area-Specific Methylation Signatures Mark Patients With Schizophrenia

Valentina Morgera, Antonia Feola, Teresa Improda, Antonio Pezone

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: DNA methylation in healthy and disease; methyl-CpG's configurations; schizophrenia

Online: 5 January 2024 (07:17:28 CET)

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Preprint HYPOTHESIS | doi:10.20944/preprints202401.0111.v1

Repair and misrepair of telomeric DNA in dynamic interactions with PML nuclear bodies and lamin B1. A gear-wheel nuclear traffic.

Kristine Salmina, Fēlikss Rūmnieks, Dace Pjanova, Jekaterina Erenpreisa

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cancer; cellular senescence; telomere attrition; alternative telomere lengthening; PML nuclear bodies; lamin B1; ALU; surface heterochromatin; nuclear rotation; chromothripsis

Online: 3 January 2024 (05:45:38 CET)

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Preprint REVIEW | doi:10.20944/preprints202401.0019.v1

Telomere Regulation by Metabolism Reprogramming

Maria P. Rubtsova, Denis A Nikishin, Maria S Koriagina, Andrey V Vasiliev, Olga A Dontsova

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: telomere; development; ALT; telomerase; metabolism; glycolysis; OXPHOS

Online: 3 January 2024 (03:54:51 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2314.v1

Identification of a Gene Signature That Predicts Dependence upon YAP/TAZ-TEAD

Ryan Kanai, Emily Norton, Patrick Stern, Richard O. Hynes, John M. Lamar

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: YAP; TAZ; WWTR1; TEAD; metastasis; melanoma; Hippo; Gene Signature

Online: 29 December 2023 (14:38:27 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0250.v3

Membrane-Associated Ubiquitin Ligase RNF152 Orchestrates Melanogenesis via Tyrosinase Ubiquitination

Ryota Ueda, Rina Hashimoto, Yuki Fujii, José C.J.M.D.S. Menezes, Hirotaka Takahashi, Hiroyuki Takeda, Tatsuya Sawasaki, Tomonori Motokawa, Kenzo Tokunaga, Hideaki Fujita

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: lysosome; melanogenesis; melanosome; RNF152; tyrosinase; ubiquitin ligase

Online: 28 December 2023 (07:13:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.2084.v1

Suitability of Gelatin Methacrylate and Hydroxyapatite Hydrogels for 3D Bioprinted Bone Tissue

Paul Stolarov, Jonathan De Vries, Sean Stapleton, Lauren Morris, Kari Martyniak, Thomas Kean

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: 3D Bioprinting; Hydroxyapatite; GelMA; Bone; DoE; Design of Experiments; bone bioprinting

Online: 27 December 2023 (11:01:23 CET)

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Background: Complex bone defects are challenging to treat. Autografting is the gold standard for regenerating bone defects, however limitations include donor site morbidity and increased surgical complexity. Advancements in 3D bioprinting (3DBP) offer a promising alternative for viable bone grafts. Methods: Design of Experiments (DoE) was used to design 12 hydrogel bioinks of various Gelatin meth-acrylate/hydroxyapatite (GelMA/HA) concentrations. These bioinks were assessed for pipettability and equilibrium modulus. An optimal bioink was designed using the DoE data to produce the greatest stiffness while still being pipettable. Two bioinks, the DoE designed maximal stiff-ness and the experimentally defined maximal stiffness vs. a literature based control were then printed using a 3D bioprinter and assessed for print fidelity. The resulting hydrogels were combined with human bone-marrow-derived mesenchymal stromal cells (hMSCs) and evaluated for cell viability. Results: DoE ANOVA analysis indicated that the augmented 3 level factorial design model used was a good fit (p < 0.0001). Using the model, DoE correctly predicted that a composite hydrogel consisting of 12.3% GelMA, 15.7% HA, and 2% Gelatin would produce the maximum equilibrium modulus while still being pipettable. The hydrogel with the most optimal print fidelity was 10% GelMA, 2% HA, and 5% Gelatin. There were no significant differences in cell viability within hydrogels from day 2 to day 7 (p > 0.05). There was however a significantly lower cell viability in the gel composed of 12.3% GelMA, 15.7% HA, and 2% gelatin compared to the other gels with lower HA concentration (p < 0.05), showing that higher HA or print pressure may be cytotoxic within hydrogels. Conclusions: Extrusion-based 3DBP offers significant advantages for bone-tissue implants due to its high customizability. This study demonstrates it is possible to create printable bone-like grafts from GelMA and HA with increased HA levels, favorable mechanical properties (145kPa) and >80% cell viability.

Preprint REVIEW | doi:10.20944/preprints202312.1922.v1

Title: Chromosome Division during Embryogenesis – Is Everything under Control? And Does the Size Really Matter?

Adela Horakova, Marketa Konecna, Martin Anger

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Spindle; chromosome division; segregation errors; Spindle Assembly Checkpoint; embryo; CDK1, cell size; aneuploidy

Online: 26 December 2023 (08:57:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1827.v1

Sex as a critical variable in basic and pre-clinical studies of fibrodysplasia ossificans progressiva

Lorraine N Burdick, Amanda H Delvichio, L. Russell Hanson, Brenden B Griffith, Keith R Bouchard, Jeffrey W Hunter, David J Goldhamer

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: sex dimorphism; fibrodysplasia ossificans progressiva; FOP; ACVR1; ALK2; antibody therapeutics; heterotopic ossification; activin A; fibro-adipogenic progenitors; FAPs; BMP signaling

Online: 25 December 2023 (11:25:24 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.1772.v1

DNA Methylation and MicroRNAs as Biomarkers in Hereditary Nonpolyposis of Colorectal Cancer Patients: A Systematic Review

Gregorio Rangel

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: colorectal cancer; DNA methylation; HNCC; microRNA; Medline; PubMed; scopus; Cochrane; systematic review

Online: 25 December 2023 (11:17:25 CET)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202312.1424.v2

Past Epigenetic Information Archive with Blockchain Data Architecture in a Biological Cellular Substructure

Jung In Choi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: epigenetic information; epigenetic modification; blockchain; cellular network; data archive; evolution; data immutability; distributed ledger; p2p communication; cellular substructure

Online: 25 December 2023 (10:26:10 CET)

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Past epigenetic information refers to the epigenetic modifications that have occurred in the past have since been updated. These modifications may no longer be present and not actively influencing on cellular functions. When past epigenetic modifications disappear, the precise records of those modifications typically may be vanish. If it is indeed true, a critical question arises: Can an organism sustain continuous survival and evolution without the information about past epigenetic modifications up to the present? It is reasonable to assume that among the information about past modifications, there could be crucial data essential for survival and evolution. If all these experiences were to vanish, rendering them unknowable in the present, it would undoubtedly pose significant challenges to survival and evolution. Therefore, the argument that such epigenetic information has been securely stored somewhere within the cell is not merely speculative but likely a factual reality.In this study, a new term “Epigenetic Archive”, is introduced in biology. It refers to a data repository that encompasses all experience data on past epigenetic modifications. The argument presented here underscores the importance of Epigenetic Archive and the need for its secure preservation. According to the argument, the information that cells experience should be continuously and securely stored, and any distortion of this information is said to be inconsistent with the principles of evolution and survival. This information is seen as evidence of a cell's successful existence up to the present, and it is emphasized that this successful record must be preserved. From this perspective, Epigenetic Archive should be designed to safely store past biological experiences and link this information securely to the present data. This study proposes that the data architecture in Epigenetic Archive is indeed akin to the principles of blockchain technology, particularly in terms of immutability and a distributed ledger securely preserving information across generations and ensuring that all cells possess consistent epigenetic information across a biological organism.The applications and implications of such an analogy of blockchain to Epigenetics could be an original contribution for future research on biology and genetics.

Preprint REVIEW | doi:10.20944/preprints202312.1432.v1

Contact Inhibition and Mechanotransduction

Fumihiko Nakamura

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: contact inhibition; contact inhibition of locomotion; contact inhibition of proliferation; mechanotransduction; filamin; mechanical force; YAP; gene expression; proteomics

Online: 19 December 2023 (11:43:53 CET)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202312.1424.v1

Past Epigenetic Information Archive with Blockchain Data Architecture in a Biological Cellular Substructure

Jung In Choi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: epigenetic information; epigenetic modification; blockchain; cellular network; data archive; evolution; immutability; distributed ledger

Online: 19 December 2023 (09:19:38 CET)

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Past epigenetic information refers to the epigenetic modifications that have occurred in the past have since been updated. These modifications may no longer be present and not actively influencing on cellular functions. When past epigenetic modifications disappear, the precise records of those modifications typically may be vanish. If it is indeed true, a critical question arises: Can an organism sustain continuous survival and evolution without the information about past epigenetic modifications up to the present? It is reasonable to assume that among the information about past modifications, there could be crucial data essential for survival and evolution. If all these experiences were to vanish, rendering them unknowable in the present, it would undoubtedly pose significant challenges to survival and evolution. Therefore, the argument that such epigenetic information has been securely stored somewhere within the cell is not merely speculative but likely a factual reality.In this study, a new term “Epigenetic Archive”, is introduced in biology. It refers to a data repository that encompasses all experience data on past epigenetic modifications. The argument presented here underscores the importance of Epigenetic Archive and the need for its secure preservation. According to the argument, the information that cells experience should be continuously and securely stored, and any distortion of this information is said to be inconsistent with the principles of evolution and survival. This information is seen as evidence of a cell's successful existence up to the present, and it is emphasized that this successful record must be preserved. From this perspective, Epigenetic Archive should be designed to safely store past biological experiences and link this information securely to the present data. This study proposes that the data architecture in Epigenetic Archive is indeed akin to the principles of blockchain technology, particularly in terms of immutability and a distributed ledger securely preserving information across generations and ensuring that all cells possess consistent epigenetic information across a biological organism.The applications and implications of such an analogy of blockchain to Epigenetics could be an original contribution for future research on biology and genetics.

Preprint ARTICLE | doi:10.20944/preprints202312.1407.v1

Cardiomyocyte Differentiation from Mouse Embryonic Stem Cells by WNT Switch Method

Isaiah K Mensah, Martin L Emerson, Hern J Tan, Humaira Gowher

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cardiomyocytes; mesoderm; mouse embryonic stem cells; heart; in vitro differentiation; wnt signaling

Online: 19 December 2023 (07:09:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1205.v1

Pro-Fibrotic Effects of CCL18 on Human Lung Fibroblasts Are Mediated via CCR6

Kerstin Höhne, Annett Wagenknecht, Corinna Maier, Peggy Engelhard, Torsten Goldmann, Stephan J Schließmann, Till Plönes, Martin Trepel, Hermann Eibel, Joachim Mueller-Quernheim, Gernot Zissel

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: idiopathic pulmonary fibrosis; fibrogenesis; chemokine receptor; ligand-induced internalization; co-immunoprecipitation; collagen; alpha-smooth muscle actin; FGF2

Online: 15 December 2023 (16:01:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1155.v1

Extracellular Matrix Tunes the Regenerative Potential of Fetal Stem Cells

Yixuan Amy Pei, Jhanvee Patel, Ming Pei

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: fetal stem cells; decellularized extracellular matrix; differentiation; fetal nucleus pulposus cell; fetal synovium-derived stem cell

Online: 15 December 2023 (09:22:50 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.1141.v1

Selenium Compounds Affect Differently the Cytoplasmic Thiol/Disulfide State in Dermic Fibroblasts and Improve Cell Migration by Interacting With the Extracellular Matrix

Christine Kreindl, Sandra Soto-Alarcón, Miltha Hidalgo, Ana Riveros, Carolina Añazco, Rodrigo Pulgar, Omar Porras

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: hyper biosensor; sodium selenite, selenium cysteine; selenium methionine; extracellular matrix; human dermic fibroblast; cellular migration

Online: 15 December 2023 (05:24:44 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0923.v1

Brain Area-Specific Methylation Signatures Mark Patients With Schizophrenia

Valentina Morgera, Antonia Feola, Teresa Improda, Mariella Cuomo, Antonio Porcellini, Antonio Pezone

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: DNA methylation in healthy and disease; methyl-CpG's configurations; schizophrenia

Online: 13 December 2023 (03:27:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0722.v1

Human Tooth Developmental Anomaly: Odontoblastic Differentiation in Hamartomatous Calcifying Hyperplastic Dental Follicles Presenting DSP, Nestin, and HES1

Hiromasa Hasegawa, Katsumitsu Shimada, Takanaga Ochiai, Yasuo Okada

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: odontoblastic differentiation; hyperplastic dental follicle; calcifying whorled nodule; nestin; dentin sialoprotein; hairy and enhancer split 1

Online: 11 December 2023 (15:18:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0250.v2

Membrane-Associated Ubiquitin Ligase RNF152 Orchestrates Melanogenesis via Tyrosinase Ubiquitination

Ryota Ueda, Rina Hashimoto, Yuki Fujii, José C.J.M.D.S. Menezes, Hirotaka Takahashi, Hiroyuki Takeda, Tatsuya Sawasaki, Tomonori Motokawa, Kenzo Tokunaga, Hideaki Fujita

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: lysosome; melanogenesis; melanosome; RNF152; tyrosinase; ubiquitin ligase

Online: 7 December 2023 (07:42:01 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.0420.v1

Acetylation and Phosphorylation in the Regulation of Hypoxia-Inducible Factors Activities: Additional Options to Modulate Adaptations to Changes in Oxygen Levels

Martina Minisini, Emanuele Cricchi, Claudio Brancolini

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Hypoxia; phosphorylation; HIF-1 alpha; HIF-2 alpha; HIF-3 alpha, acetylation; HDACs; KATs

Online: 7 December 2023 (06:16:42 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.0364.v1

Mechanisms of Programmed Cell Death: Structural and Functional Proteomic-Pathways. A Narrative Review.

Diego Fernández-Lázaro, Begoña Sanz, Jesús Seco-Calvo

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Programmed cell death; Apoptosis; Caspases; Caspase inhibitory proteins; Mitochondrial / Intrinsic pathway; Extrinsic Pathway; Necroptosis; Autophagy

Online: 6 December 2023 (08:54:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0250.v1

Membrane-Associated Ubiquitin Ligase RNF152 Orchestrates Melanogenesis via Tyrosinase Ubiquitination

Ryota Ueda, Rina Hashimoto, Yuki Fujii, José C.J.M.D.S. Menezes, Hirotaka Takahashi, Hiroyuki Takeda, Tatsuya Sawasaki, Tomonori Motokawa, Kenzo Tokunaga, Hideaki Fujita

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: lysosome; melanogenesis; melanosome; RNF152; tyrosinase; ubiquitin ligase

Online: 5 December 2023 (14:33:53 CET)

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Preprint REVIEW | doi:10.20944/preprints202312.0206.v1

E3 Ligases Regulate Organelle Inheritance in Yeast

Keisuke Obara, Kohei Nishimura, Takumi Kamura

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: ubiquitin; E3 ligase; proteolysis; vacuole; mitochondria; peroxisome; myosin; yeast

Online: 5 December 2023 (04:48:31 CET)

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Preprint ARTICLE | doi:10.20944/preprints202312.0178.v1

Functional and Multi-Omics Analysis of an Optimized CRISPR-Mediated FURIN Depletion in Cultured Pro-Monocytic Cells

Ruiming Chua, Lijin Wang, Roshni Singaraja, Sujoy Ghosh

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: myeloid cells; FURIN protease; gene editing; functional assays; genomics; cytokine secretion

Online: 4 December 2023 (10:18:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1987.v1

The Multiple Roles of Temporal Collinearity in Hox Gene Clustering for Vertebrates, Invertebrates, Complete and Split Hox Clusters

Spyros Papageorgiou

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Hox gene collinearity; temporal collinearity; Noether Theory; self similarity; double strand break; split Hox clusters; chicken limb growth
;

Online: 1 December 2023 (03:12:17 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1972.v1

Generating Patient-Derived HCC Cell Lines Suitable for Predictive In-Vitro and In-Vivo Drug Screening by Orthotopic Transplantation

Lisa Staffeldt, Gregor Mattert, Kristoffer Riecken, Götz Rövenstrunk, Anika Volkmar, Mohamed Moustafa, Asmus Heumann, Manfred Jücker, Boris Fehse, Udo Schumacher, Stefan Lüth, Janine Kah

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: patient-derived HCC cell lines; in-vitro investigating; therapeutic testing; orthotopic transplantation

Online: 30 November 2023 (10:52:44 CET)

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Hepatocellular carcinoma (HCC) results in high mortality due to ineffective systemic therapy. Human immortalized cell lines are commonly used to study anti-tumour effects in the context of new anti-tumour therapies and tumour biology. As immortalized cell lines have limited biological relevance and heterogeneity compared to primary cells, patient-derived tumour tissues and corresponding immune cells are the gold standards for studying the complexity of individual tumour entities. However, culturing primary HCC cells has a low success rate. Here, we aimed to establish a reproducible approach to preserve the patient-derived liver cancer cells for in vitro and in vivo studies. The underlying study aimed to establish an in vitro pre-screening platform to test treatment options' effectivity and dosage, e.g., for new substances, autologous modified immune cells, or combined therapies in HCC. We initially employed 15 surgical resection specimens from patients with different HCC entities for isolation and preservation. The isolated liver cancer cells from four HCC-diagnosed patients were used for orthotopic transplantation into the healthy liver of immunodeficient mice, allowing them to grow for six months before human liver cancer cells were isolated and cultured. As a result, we generated and characterized four new primary-like liver cancer cell lines. Compared to immortalized HCC cell lines, freshly generated liver cancer cells displayed individual morphologies and heterogenous protein-level characteristics. We assessed their ability to proliferate, migrate, form spheroids, and react to common medications compared to immortalized HCC cell lines. All four liver cancer cell lines exhibit strong migration and colony-forming characteristics in vitro, comparable to extensively investigated immortalized HCC cell lines. Moreover, the four etiological different liver cancer cell lines displayed differences in the response to 5-FU, Sorafenib, Axitinib and Interferon-alpha treatment, ranking from non-responders to responders depending on the applicated medication. In sum, we generated individual patient-derived liver cancer cell lines suitable for predictive in vitro drug screenings and moreover for xenograft transplantations to realize the in vivo investigation of drug candidates. We overcame the low cultivation success rate of liver cancer cells derived from patients and analyzed their potential to serve a pre-clinical model.

Preprint ARTICLE | doi:10.20944/preprints202311.1895.v1

ID2 Promotes Lineage Transition of Prostate Cancer through FGFR and JAK-STAT Signaling

Jinxiong Zhang, Zhihao Chen, Yongxin Mao, Lu Sheng, Jianhong Wu, Yijun He, Xin Wu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: lineage transition; prostate cancer; ID2; NEPC and DNPC

Online: 29 November 2023 (15:21:48 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1805.v1

Role of HDAC6 and HDAC6 Inhibition in Colorectal Cancer

Ana Vuletić, Katarina Mirjačić Martinović, Jelena Spasić

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: HDAC6; histone deacetylases inhibitors; colorectal cancer

Online: 28 November 2023 (10:13:23 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1361.v1

Induced Pluripotent Stem Cells in Drug Discovery and Neurodegenerative Disease Modeling

Daniela Gois Beghini, Tais Hanae Kasai-Brunswick, Andrea Henriques-Pons

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: induced pluripotent stem cells; disease modeling, neurodegenerative diseases

Online: 22 November 2023 (09:39:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1139.v1

PRIMPOL and POLI Deficiencies Differentially Affect DNA Repair and Post-Irradiation Survival of Non-Small Cell Lung Cancer Cell Lines

Margarita Pustovalova, Philipp Malakhov, Zain Nofal, Irina Moroz, Taisia Trubchenkova, Anastasia Gromova, Sergey Leonov

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: non-small cell lung cancer; ionizing radiation; DNA repair; PrimPol; DNA damage tolerance; translesion synthesis; Pol iota (Polι ); clonogenic survival; cancer stem-like cells

Online: 17 November 2023 (07:47:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.1012.v1

LGR5+ Intestinal Stem Cells Display Sex Dependent Radiosensitivity

Ryan C. Zitter, Rishi Man Chugh, Payel Bhanja, Subhrajit Saha

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: LGR5+; radiosensitivity; oxidative-stress; ISCs

Online: 15 November 2023 (13:57:04 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0881.v1

Overcome Resistance in Doxorubicin-Adapted Hodgkin Lymphoma Cells With Acquired Multidrug Resistance and Improved Immunosuppressive Abilities

Naike Casagrande, Cinzia Borghese, Michele Avanzo, Donatella Aldinucci

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Hodgkin lymphoma; doxorubicin; drug resistance; cross-resistance; immunosuppression

Online: 14 November 2023 (09:57:04 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.0897.v1

The Role of Sleep in Multiple Sclerosis

Susanna Cordone, Valentina Alfonsi, Luigi De Gennaro

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: multiple sclerosis; sleep; immune system; neuroinflammation; cytokines; mitochondria; neuro-degeneration; melatonin; vitamin D; sleep-based interventions.

Online: 14 November 2023 (09:44:34 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.1417.v2

Cellular Response to an Act of Surviving Drives Tumor Evolution and Tumor Heterogeneity

Yuntian Zhang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: tumor heterogeneity; cancer progression; oncogene addiction; cancer stem cell

Online: 13 November 2023 (10:39:49 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.0734.v1

Cell Adhesion at the Tight Junctions: New Aspects and New Functions

Nicolina Wibbe, Klaus Ebnet

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: cell-cell adhesion; force sensing; junctional adhesion molecule; JAM-A; phase separation; tight junction

Online: 13 November 2023 (09:24:21 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.0588.v1

Advances of Protein Palmitoylation in Tumor Cell Deaths

Xiangyi Lin, Yuxuan Shi, Yuxin Zhan, Yuying Xing, Yu Li, Zhiqing Zhou, Guoan Chen

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: protein palmitoylation; apoptosis; autophagy; ferroptosis; pyroptosis

Online: 9 November 2023 (07:43:38 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0526.v1

iPSC-Derived Endothelial Cells Reveals LDLR-Dysfunction and Dysregulated Gene Expression Profiles in Familial Hypercholesterolemia

Irina S. Zakharova, Alexander I. Shevchenko, MHD Amin Arssan, Alexei A Sleptcov, Maria S. Nazarenko, Aleksei A. Zarubin, Nina V. Zheltysheva, Vlada A. Shevchenko, Narek A. Tmoyan, Shoraan B. Saaya, Marat V. Ezhov, Valery V. Kukharchuk, Yelena V. Parfyonova, Suren M. Zakian

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: familial hypercholesterolemia; LDLR; patient-specific iPSCs; directed differentiation; endothelial cells

Online: 8 November 2023 (16:14:59 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0533.v1

HIF-1α/MMP-9 Axis Is Required for the Early Phases of Skeletal Myoblast Differentiation under Normoxia Condition In Vitro

Flaminia Chellini, Alessia Tani, Martina Parigi, Francesco Palmieri, Rachele Garella, Sandra Zecchi-Orlandini, Roberta Squecco, Chiara Sassoli

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: electrophysiology; HIF; immunofluorescence; MMP-9; myogenesis; myoblasts; normoxia; regeneration; satellite cells; skeletal muscle.

Online: 8 November 2023 (10:31:12 CET)

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Preprint ARTICLE | doi:10.20944/preprints202311.0069.v1

Unexpected Differences in the Speed of Non-malignant versus Malignant Cell Migration Reveal Differential Basal ATP Levels

Bareun Kim, Anthony T. Lopez, Indhujah Thevarajan, Maria F. Osuna, Monica Mallavarapu, Boning Gao, Jihan K. Osborne

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Cell Migration; Cancer cell migration; normal epithelial cell motility

Online: 1 November 2023 (11:18:02 CET)

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Preprint REVIEW | doi:10.20944/preprints202310.1423.v1

A Mechanistic Discussion on Oxidative Stress and Ferroptosis

Shreya Mukherjee, Chayan Munshi

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Oxidative stress; Ferroptosis; Iron; Cell death; Lipid peroxidation

Online: 23 October 2023 (09:42:29 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.1417.v1

Cellular Response to an Act of Surviving Drives Tumor Evolution and Tumor Heterogeneity

Yuntian Zhang

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: tumor heterogeneity; cancer progression; oncogene addiction; cancer stem cell

Online: 23 October 2023 (08:47:58 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0988.v1

Colorectal Cancer Stem Cells and Targeted Agents

Haobin Zhao, Ruining Han, Zhankun Wang, Junfang Xian, Xiaosu Bai

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Colorectal cancer stem cells; LGR5; Wnt signaling pathway; Single-cell omics technology

Online: 17 October 2023 (05:48:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0839.v1

An Overview of Golgi Membrane-Associated Degradation and Its Detection Methods

Hajime Tajima Sakurai, Satoko Arakawa, Hirofumi Yamaguchi, Satoru Torii, Shinya Honda, Shigeomi Shimizu

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: GOMED; alternative autophagy; Golgi

Online: 13 October 2023 (07:47:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0843.v1

Heavy Metals in the Follicular Fluid Affect the Ultrastructure of the Human Mature Cumulus-Oocyte Complex

Selenia Miglietta, Loredana Cristiano, Ezio Battaglione, Guido Macchiarelli, Stefania Annarita Nottola, Maria Paola Marco, Flavia Costanzi, Mauro Schimberni, Nicola Colacurci, Donatella Caserta, Giuseppe Familiari

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: heavy metals; lead; cadmium; oocyte; cumulus cells; human; electron micros

Online: 13 October 2023 (04:30:41 CEST)

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It is known that exposure to heavy metals such as lead (Pb) and cadmium (Cd) has several adverse effects, particularly on the human reproductive system. Pb and Cd have been associated with infertility in both men and women. In pregnant women, they have been associated with spontaneous abortion, preterm birth, and impairment of the development of the fetus. Since these heavy metals come from both natural and anthropogenic activities and their harmful effects have been observed even at levels of exposure, their exposure remains a public health issue, especially for the reproductive system. Given this, the present study aimed to investigate the potential reproductive effects of Pb and Cd levels in the follicular fluid (FF) of infertile women and no-smokers exposed to heavy metals for professional reasons or living in rural areas near landfills and waste disposal areas, to correlate the intrafollicular presence of these metals with possible alterations in the ultrastructure of human cumulus-oocyte complexes (COCs), probably responsible of infertility. Blood and FF metals were measured using atomic absorption spectrometry. COCs corresponding to each FF analyzed were subjected to ultrastructural analyses by transmission electron microscopy. We demonstrated for the first time that intrafollicular levels of Pb (0.66 µg/dL to 0.85 µg/dL) and Cd (0.26 µg/L - 0.41 µg/L) could be associated with morphological alterations of both the oocyte and CCs ultrastructure. Since blood Cd levels (0.54 µg/L - 1.87 µg/L) were above the current reference values established by the guidelines of the Agency for Toxic Substances and Disease Registry (ATSDR) and the Environmental Protection Agency (EPA), whereas blood Pb levels (1.28 µg/dL - 3.98 µg/dL) were below the ATSDR reference values we believe that these alterations could be due especially to Cd, even if we cannot exclude a possible additional effect of Pb. Our results highlighted that oocytes were affected in maturation and quality, whereas CCs showed scarcely active steroidogenic elements. Regressing CCs, with cytoplasmic alterations, were also numerous. According to Cd's endocrine-disrupting activity, the poor steroidogenic activity of CCs might correlate with delayed oocyte cytoplasmic maturation. So, we conclude that levels of heavy metals in the blood and the FF might negatively affect fertilization, embryo development, and pregnancy, compromising oocyte competence to fertilization both directly and indirectly, impairing CC steroidogenic activity, and inducing CC apoptosis.

Preprint ARTICLE | doi:10.20944/preprints202310.0751.v1

Cyclin B Export to the Cytoplasm via the Nup62 Subcomplex and Its Subsequent Rapid Nuclear Import Are Required for the Initiation of Drosophila Male Meiosis

Kanta Yamazoe, Yoshihiro H. Inoue

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Drosophila; male meiosis; cyclin B; Cyclin-dependent kinase 1; Cyclin-dependent kinase inhibitors

Online: 12 October 2023 (05:32:28 CEST)

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Preprint REVIEW | doi:10.20944/preprints202310.0363.v1

Novel insights into the molecular mechanisms governing embryonic epicardium formation

Rita Carmona, Carmen Lopez-Sanchez, Virginio Garcia-Martinez, Virginio García-López, Ramón Muñoz-Chápuli, Estefanía Lozano-Velasco, Diego Franco

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: proepicardium, epicardium, transcriptional, post-transcriptional

Online: 9 October 2023 (15:04:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0391.v1

Proteomic analysis of human iPSC derived neural stem cells and motor neurons identifies proteasome structural alterations.

Iñaki Álvarez, Adrián Tirado-Herranz, Belén Alvarez Palomo, Jordi Requena Osete, Michael John Edel

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Proteomics, Induced pluripotent stem cells, Differentiation, Neural Stem Cells, Motor Neurons, 26S proteasome, Proteasome.

Online: 9 October 2023 (10:51:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0373.v1

Integrins Can Act As Suppressors of Ras-Mediated Oncogenesis in the Drosophila Wing Disc Epithelium

Maria D. Martín-Bermudo, Ana Martínez-Abarca Millán

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: drosophila integrins; cancer; cell growth; invasion

Online: 9 October 2023 (04:37:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0385.v1

Epigenetic Profiling of PTPN11 Mutant JMML Hematopoietic Stem and Progenitor Cells Reveals an Aberrant Histone Landscape

Roshani Sinha, Mai Dvorak, Ananthakrishnan Ganesan, Larry Kalesinskas, Charlotte M. Niemeyer, Christian Flotho, Kathleen M Sakamoto, Norman Lacayo, Rachana Vinay Patil, Rhonda Perriman, Alma-Martina Cepika, Y. Lucy Liu, Alex Kuo, Paul Utz, Purvesh Khatri, Alice Bertaina

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: histone modifications; pediatric leukemia; chromatin acessibility; epigenetics; mass cytometry; JMML; novel targets

Online: 7 October 2023 (09:49:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0303.v1

Resveratrol Reduces ROS by Increasing GSH in Vitrified Sheep Embryos

Andrea Carolina González-Garzón, Julio Porfirio Ramón-Ugalde, Demetrio Alonso Ambríz-García, José Roberto Vazquez-Avendaño, José Ernesto Hernández-Pichardo, José Luis Rodríguez-Suástegui, César Cortez-Romero, María del Carmen Navarro-Maldonado

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Resveratrol; ROS; GSH; vitrification

Online: 6 October 2023 (05:57:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.0194.v1

SARS-CoV-2 Impact on Red Blood Cell Morphology

Kirill Kondratov, Alexander Artamonov, Vladimir Mikhailovskii, Anastasiya Velmiskina, Sergey Mosenko, Evgeniy Grigoryev, Anna Anisenkova, Yuri Nikitin, Svetlana Apalko, Natalya Sushentseva, Andrey Ivanov, Sergey Shcherbak

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: COVID-19; erythrocyte; red blood cells; cytokine storm; severe COVID-19; low voltage scanning electron microscopy; erythrocyte size

Online: 4 October 2023 (04:10:30 CEST)

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Biology and Life Sciences