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Preprint REVIEW | doi:10.20944/preprints202209.0208.v1

Artificial Intelligent-Powered Detection of Tuberculosis

Abdullahi Umar Ibrahim, Manal Salah Babiker, Yeni Wahyuni Hartati, Yeni Wahyuni Hartati

Subject: Life Sciences, Microbiology Keywords: Tuberculosis; Artificial Intelligence; Machine Learning; Deep Learning; Transfer Learning; Computer-aided Diagnosis

Online: 14 September 2022 (12:00:44 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202209.0147.v1

Discovery, Biosynthesis and Biological Activity of a Succinylated Myxochelin from the Myxobacterial strain MSr12020

Dorothy A. Okoth, Joachim J. Hug, Ronald Garcia, Rolf Müller

Subject: Life Sciences, Microbiology Keywords: Myxochelin; Myxobacteria; Biosynthesis; Natural Products; Secondary Metabolites; Succinylation; Siderophore; Succinyl-coenzyme A

Online: 12 September 2022 (12:22:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0143.v1

Cannabis sativa CBD Extract Exhibits Synergy with Broad-Spectrum Antibiotics against Salmonella typhimurium

Logan Gildea, Joseph Ayariga, Junhuan Xu, Robert Villafane, Robertson Boakai, Michelle Samuel-Foo, Olufemi Ajayi

Subject: Life Sciences, Microbiology Keywords: Salmonella; novel antibacterial agents; cannabidiol; co-therapy; bacterial genetics

Online: 12 September 2022 (09:55:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202209.0098.v1

Comparison of Two Bacterial Characterization Techniques for the Genomic Analysis of Aquatic Environments

Roberto Bonelly, Victor V. Calderón, Confesora Pinales, Irene Ortiz, Argenys Ovando, Willy Lara, Santo E. Mateo-Perez, Oscar Cardenas-Alegría, Rommel T. Jucá Ramos, Edian F. Franco, Luis O. Maroto

Subject: Life Sciences, Microbiology Keywords: MALDI-TOF; DNA Sequencing; environment; bacteria; Microbiome; bioinformatics; Dominican Republic

Online: 7 September 2022 (04:26:40 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0519.v1

Historic and Prehistoric Epidemics: An Overview of Sources Available for the Study of Ancient Pathogens

Antoinette Cornelia Van der Kuyl

Subject: Life Sciences, Microbiology Keywords: prehistory; history; archeobiology; paleovirology; EVEs; ancient DNA; paleogenomics; pathology collections; historic publications

Online: 30 August 2022 (10:29:39 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0478.v1

Purification of High Molecular-Weight Antibacterial Proteins of Insect Pathogenic Brevibacillus Laterosporus Isolates

Tauseef K. Babar, Travis R. Glare, John G. Hampton, Mark R. H. Hurst, Josefina O. Narciso, Amy Beattie

Subject: Life Sciences, Microbiology Keywords: antibacterial proteins; encapsulating protein; high molecular-weight bacteriocins; insect patho-genic bacterium; phage tail-like protein; purification methods

Online: 29 August 2022 (09:00:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0466.v1

Analysis of the virulence and inflammatory markers elicited by Enteroaggregative Escherichia coli isolated from clinical and non-clinical sources in an experimental infection model, India

Vinay Modgil, ChanderDeo Narayan, Harpreet Kaur, Vivek kumar Yadav, Naveen Chaudhary, Vishal Kant, Balwinder Mohan, Alka Bhatia, Neelam Taneja

Subject: Life Sciences, Microbiology Keywords: Keywords: Enteroaggregative Escherichia coli, virulence, inflammatory markers, Phylogeny, Multiplex PCR.

Online: 29 August 2022 (04:49:31 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0457.v1

Metagenomics Next Generation Sequencing (mNGS): An Exciting Tool for Early and Accurate Diagnosing Plant Fungal Pathogens

F. Şeyma Gökdemir, Özlem D. İşeri, Abhishek Sharma, Premila Achar, Füsun Eyidoğan

Subject: Life Sciences, Microbiology Keywords: mNGS; Metabarcoding; ITS; Biyoinformatic Analysis; Fungal Pathogenesis

Online: 26 August 2022 (09:50:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0425.v1

Mycobacterium Bovis Strain Ravenel is Attenuated in Cattle

Syeda Anum Hadi, Evan P Brenner, Mitchell V Palmer, Wade Ray Waters, Tyler C Thacker, Catherine Vilcheze, Michelle H Larsen, William R Jacobs, Jr., Srinand Sreevatsan

Subject: Life Sciences, Microbiology Keywords: bovine tuberculosis; TB; attenuation; genomes; immunopathogenesis; SNPs

Online: 25 August 2022 (07:18:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0381.v1

Impact of the Temperature in Endophytic Ascomycota Isolated from Antarctic Hair-Grass

Fabíola Lucini, Guilherme Afonso Kessler De Andrade, Filipe de Carvalho Victoria, Margeli Pereira De Albquerque

Subject: Life Sciences, Microbiology Keywords: Endophitic Fungi; Phylopgeny; Biodiversity; Molecular Biology; Growth experiments

Online: 22 August 2022 (11:00:55 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0236.v1

How to Shut Down Transcription in Archaea During Virus Infection

Simona Pilotto, Finn Werner

Subject: Life Sciences, Microbiology Keywords: Archaea; transcription inhibition; RNA polymerase; viruses; evolution; antibiotics

Online: 12 August 2022 (11:25:05 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0145.v1

Interspecies and Intrastrain Interplay Among Leishmania spp. Parasites

Bruna Dias Das Chagas, Thaís Martins Pereira, Lilian Motta Cantanhêde, Gabriela Pereira Da Silva, Mariana Côrtes Boité, Luiza de Oliveira Ramos Pereira, Elisa Cupolillo

Subject: Life Sciences, Microbiology Keywords: Leishmania; co-infections; mixed infections; co-culture; hybrid; intercellular communication

Online: 8 August 2022 (10:20:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0143.v1

Molecular Epidemiology and Virulence of Non-Typhoidal Salmonella in Armenia

Anahit Sedrakyan, Zhanna Ktsoyan, Karine Arakelova, Zaruhi Gevorgyan, Magdalina Zakharyan, Shoghik Hakobyan, Alvard Hovhannisyan, Arsen Arakelyan, Rustam Aminov

Subject: Life Sciences, Microbiology Keywords: non-typhoidal Salmonella; ERIC-PCR typing; WGS; virulence-related genes; SPIs; Salmonella virulence plasmids; prophages

Online: 8 August 2022 (10:07:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0133.v1

Arbuscular Mycorrhizal Fungi and Biofilm Forming Bacteria Act Synergistically to Modulate Proline Metabolism, Antioxidant Defense System and Aquaporin Genes Expression Under Drought Stress

Iqra Siddique, Tariq Shah, Ahmad Ali, Ijaz Ahmad, Roberto D'amato, Fazal Munsif

Subject: Life Sciences, Microbiology Keywords: Abscisic acid biosynthetic genes; aquaporins; Biofilm forming bacteria; growth traits; oxidative injury; Tomato

Online: 8 August 2022 (05:48:17 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202208.0132.v1

Mechanism-based Approach to New Antibiotic Producers Screening among Actinomycetes in the Course of Civil Science

Inna A. Volynkina, Yuliya V. Zakalyukina, Vera A. Alferova, Albina R. Belik, Daria K. Yagoda, Arina A. Nikandrova, Yuliya A. Buyuklyan, Andrei V. Udalov, Evgenii V. Golovin, Maxim A. Kryakvin, Dmitrii A. Lukianov, Mikhail V. Biryukov, Petr V. Sergiev, Olga A. Dontsova, Ilya A. Osterman

Subject: Life Sciences, Microbiology Keywords: civil science; antibiotic producers screening; actinomycetes; reporter systems; chartreusin; pikromycin

Online: 8 August 2022 (05:27:03 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0036.v1

Virulence Characteristics and Distribution of the Pathogen Listeria Ivanovii in the Environment and in Food

Franca Rossi, Valerio Giaccone, Giampaolo Colavita, Carmela Amadoro, Francesco Pomilio, Paolo Catellani

Subject: Life Sciences, Microbiology Keywords: Listeria ivanovii; pathogenicity; virulence characters; environmental distribution; occurrence in food

Online: 2 August 2022 (04:41:19 CEST)

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Preprint REVIEW | doi:10.20944/preprints202208.0032.v1

Microbiota in a Long Survival Discourse with the Human Host

Joseph Ayariga, Iddrisu Ibrahim, Logan Gildea, James Abugri, Robert Villafane

Subject: Life Sciences, Microbiology Keywords: Microbiota; Microbiome; Adhesions; Pili; Curli; Enterotoxins

Online: 2 August 2022 (04:07:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0459.v1

Associated Risk Factors and Pathogen Burden among ICU Patients in a Tertiary Care Hospital in Hail Saudi Arabia with Particular Reference to β-Lactamases Profile

Saleem Mohd, Azharuddin Sajid Syed Khaja, Ashfaque Hossain, Fahaad Alenazi, Kamaleldin B Said, Soha Abdallah Moursi, Homoud Abdulmohsin Almalaq, Hamza Mohamed, Ehab Rakha, Mohammed Salem Alharbi, Kauser Usman

Subject: Life Sciences, Microbiology Keywords: ICU; VAP; ESBL; Klebsiella pneumoniae; Acinetobacter baumannii

Online: 29 July 2022 (10:15:38 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0425.v1

Anti-quorum Sensing Potential of Ethanolic Extracts of Aromatic Plants From Cyprus Flora

Tolis Panayi, Yiannis Sarigiannis, Elena Mourelatou, Evroula Hapeshi, Christos Papaneophytou

Subject: Life Sciences, Microbiology Keywords: quorum sensing; autoinducer; organic plant extracts; biofilms; swimming motility; swarming motility

Online: 27 July 2022 (15:07:29 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0365.v1

Advances in Enteropathogen Control Throughout the Meat Chicken Production Chain

Jack G Stewart, Anthony Pavic

Subject: Life Sciences, Microbiology Keywords: Salmonella; Campylobacter; poultry; review; vaccines; processing; farm-to-fork; broilers; meat birds; production

Online: 25 July 2022 (09:24:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0320.v1

Fine Epitope Mapping of the Vibrio cholera Toxins A, B, and P and an ELISA Assay

Salvatore Giovanni De-Simone, Paloma Napoleão-Pêgo, Priscilla Simone Gonçalves, Guilher Curty Lechuga, Sergian Vianna Cardozo, David William Provance, Carlos Meicis Morel, Flavio Rocha Silva

Subject: Life Sciences, Microbiology Keywords: Cholera toxin; B-cell epitope; peptide microarray; synthetic peptide; MAPs; ELISA

Online: 21 July 2022 (10:32:40 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0286.v1

Impact of COVID-19 Vaccination on Healthcare Worker Infection Rate and Outcome during SARS-CoV-2 Omicron Variant Outbreak in Hong Kong

Sze Tsing Jonpaul Zee, Lam Fung Kwok, Carmen Ka Man Kee, Ling Hiu Fung, Luke Wing Pan Luk, Chris Tsun Leung Chan, Alex Chin Pang Leung, Bella Pik Wa YU, Jhan Raymond L Hung, Kit Ying SzeTo, Queenie Wai Leng Chan, Bone Siu Fai Tang, Ada Wai Chi Lin, Edmond Shiu Kwan Ma, Koon Hung Lee, Chor Chiu Lau, Raymond Wai Hung Yung

Subject: Life Sciences, Microbiology Keywords: SARS-CoV-2; Omicron variant of concern; homologous boosting; heterologous boosting; Coro-naVac; BNT162b2; healthcare worker; return-to-work

Online: 19 July 2022 (10:15:39 CEST)

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Preprint REVIEW | doi:10.20944/preprints202207.0245.v1

Biological and Cosmetical Importance of Fermented Raw Materials: An Overview

Weronika Majchrzak, Ilona Motyl, Krzysztof Śmigielski

Subject: Life Sciences, Microbiology Keywords: bioferments; natural cosmetics; fermentation; bioactive compounds

Online: 18 July 2022 (03:38:33 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0204.v1

The Sinorhizobium Meliloti Nsps-Mbaa System affects Biofilm Formation, Exopolysaccharide Production and Motility in Response to Specific Polyamines

Víctor M. Chávez-Jacobo, Víctor A. Becerra-Rivera, Gabriela Guerrero, Michael F. Dunn

Subject: Life Sciences, Microbiology Keywords: cyclic di-GMP; polyamine sensing; NspS-MbaA. S. meliloti; biofilm; exopolysaccharides; motility

Online: 14 July 2022 (03:59:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0157.v1

Description of Methylomonas Aquatica Sp. Nov., Isolated from a Tropical Indian Rice Rhizosphere

Monali Rahalkar

Subject: Life Sciences, Microbiology Keywords: Methylococcaceae; methanotroph; Indian rice field; Methylmonas; novel species

Online: 11 July 2022 (09:56:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202207.0113.v1

Functional Diversity of Microbial Communities in the Soybean (Glycine max L.) Rhizosphere from Free State, South Africa

Titilope Tinu Ajiboye, Ayansina Segun Ayangbenro, Olubukola Oluranti Babalola

Subject: Life Sciences, Microbiology Keywords: microbial communities; plant-microbe interractions; rhizodeposition; SEED subsystem; shotgun metagenomics

Online: 7 July 2022 (06:05:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0389.v1

Dynamics of Gut Microbiome and Transcriptome in Korea Native Ricefish (Oryzias latipes) during Chronic Antibiotics Exposure

Ju Bin Yoon, Sungmin Hwang, Jun Hyeok Yang, Seungki Lee, Woo Young Bang, Ki Hwan Moon

Subject: Life Sciences, Microbiology Keywords: Ricefish; Microbiome; Ampicillin; Erythromycin; Immune and Stress-Related Genes

Online: 29 June 2022 (03:05:46 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202206.0374.v1

Highly Virulent and Multidrug-Resistant Escherichia Coli Sequence Type 58 from a Sausage in Germany

Elias Eger, Marielle Domke, Stefan E. Heiden, Madeleine Paditz, Veronika Balau, Christiane Huxdorff, Dirk Zimmermann, Timo Homeier-Bachmann, Katharina Schaufler

Subject: Life Sciences, Microbiology Keywords: antimicrobial resistance; CTX-M-1; Enterobactericeae,; Escherichia coli; extended-spectrum β-lactamase; food safety; IncI1; next-generation sequencing; One Health; virulence

Online: 28 June 2022 (04:18:22 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0260.v1

Canine Fecal Microbiota Transplantation- Current Application and Possible Mechanisms

Gulbahar Tursun, Mihrigul Mattursun, Maimaiti Tuniyazi

Subject: Life Sciences, Microbiology Keywords: canine; fecal microbiota transplantation; treatment; mechanism

Online: 20 June 2022 (05:49:24 CEST)

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Preprint REVIEW | doi:10.20944/preprints202206.0229.v1

It Takes Two to Tango; A Review of Oncogenic Virus and Host Microbiome Associated Inflammation on Head and Neck Cancer

Mallory G. McKeon, Jean-Nicolas Gallant, Young J. Kim, Suman R. Das

Subject: Life Sciences, Microbiology Keywords: pharynx; oncogenic virus; oropharyngeal cancer; nasopharyngeal cancer; human papillomavirus (HPV); Epstein–Barr virus (EBV); microbiome; infection; inflammation; carcinogenesis

Online: 16 June 2022 (04:27:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0196.v1

Phenotypic and Genotypic Detection of Biofilm-Forming Staphylococcus aureus from Different Food Sources in Bangladesh

Fatimah Mohammad Ballah, Md. Saiful Islam, Md. Liton Rana, Farhana Binte Ferdous, Rokeya Ahmed, Pritom Kumar Pramanik, Jarna Karmoker, Samina Ievy, Md. Abdus Sobur, Mahbubul Pratik Siddique, Mst. Minara Khatun, Marzia Rahman, Md. Tanvir Rahman

Subject: Life Sciences, Microbiology Keywords: biofilm formation; S. aureus; CRA plating test; CVMP test; ica genes; bap gene; foods; public health; Bangladesh

Online: 14 June 2022 (06:10:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0152.v1

Catheter-associated Urinary Tract Infection in Intensive Care Unit Patients at a Tertiary Care Hospital, Hail, Kingdom of Saudi Arabia

Saleem Mohd, Azharuddin Sajid Syed Khaja, Ashfaque Hossain, Fahaad Alenazi, Kamaleldin B. Said, Soha Abdallah Moursi, Homoud Abdulmohsin Almalaq, Hamza Mohamed, Ehab Rakha, Sunit Kumar Mishra

Subject: Life Sciences, Microbiology Keywords: Catheter-associated urinary tract infections (CAUTI); Klebsiella pneumoniae; Hospital-acquired infections; Proteus mirabilis; Pseudomonas aeruginosa; antibiogram

Online: 10 June 2022 (07:53:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202206.0141.v1

Molecular Characterization and Antibiogram of Acinetobacter Baumannii Clinical Isolates Recovered From the Patients With Ventilator-Associated Pneumonia

Saleem Mohd, Azharuddin Sajid Syed Khaja, Ashfaq Hossain, Fahaad Alenazi, Kamaleldin B. Said, Soha Abdallah Moursi, Homoud Abdulmohsin Almalaq, Hamza Sharif Mohamed, Ehab Rakha

Subject: Life Sciences, Microbiology Keywords: Acinetobacter baumannii; XDR; IMP-1; VIM-2; NDM-1; VAP; ICU; Hospital-acquired infections

Online: 9 June 2022 (10:58:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0362.v1

Development of a Biotechnology Platform for the Fast-Growing Cyanobacterium Synechococcus sp. PCC 11901

David John Lea-Smith, Lauren A Mills, Jose Angel Moreno-Cabezuelo, Artur Wlodarczyk, Angelo J Victoria, Rebeca Mejias, Anja Nenninger, Simon Moxon, Paolo Bombelli, Tiago Toscano Selao, Alistair McCormick

Subject: Life Sciences, Microbiology Keywords: Synechococcus sp. PCC 11901; CodA selection; SacB selection; Vitamin B12; Photosynthesis, Photoinhibition; Comparative genomics; Cellular metabolism

Online: 26 May 2022 (10:30:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0003.v2

Overexpression of a Membrane Transport System MSMEG_1381 and MSMEG_1382 Confers Multidrug Resistance in Mycobacterium smegmatis

Salini S., Balaji Muralikrishnan, Sinchana Bhat, Sudeep Ghate, Shyama Prasad Rao, R. Ajay Kumar, Krishna Kurthkoti

Subject: Life Sciences, Microbiology Keywords: Drug resistance; Efflux pump; TetR transcriptional regulator; Mycobacteria; MmpL

Online: 24 May 2022 (11:32:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0321.v1

Pneumococcal Carriage in Infants Post-PCV10 Introduction in Pakistan: Results from Serial Cross-Sectional Surveys

Amala Khan, Shahira Shahid, Muhammad Imran Nisar, Farah Khalid, Muhammad Farrukh Qazi, Sheraz Ahmed, Furqan Kabir, Aneeta Hotwani, Sahrish Muneer, Syed Asad Ali, Cynthia G. Whitney, Anita K.M. Zaidi, Fyezah Jehan

Subject: Life Sciences, Microbiology Keywords: Streptococcus pneumoniae; 10-valent Pneumococcal Conjugate Vaccine; Pakistan

Online: 24 May 2022 (04:50:14 CEST)

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Preprint REVIEW | doi:10.20944/preprints202205.0199.v1

The Role of Early Life Gut Microbiota Composition in the Development of Allergic Diseases

Maimaiti Tuniyazi, Shuang Li, Xiaoyu Hu, Yunhe Fu, Naisheng Zhang

Subject: Life Sciences, Microbiology Keywords: allergy; microbiome; early life

Online: 16 May 2022 (08:05:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0186.v1

Fungi in Microbial Culture Collections and Their Metabolites

Alexander Vasilenko, Natalya Ivanushkina, Galina Kochkina, Svetlana Ozerskaya

Subject: Life Sciences, Microbiology Keywords: collections of microorganisms; data bases; fungal diversity; metabolites

Online: 13 May 2022 (09:42:11 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0175.v1

Heteroaryl-Ethylenes as New Effective Agents for High Priority Gram-Positive and Gram-Negative Bacterial Clinical Isolates

Dalida Angela Bivona, Alessia Mirabile, Carmelo Bonomo, Paolo Giuseppe Bonacci, Stefano Stracquadanio, Andrea Marino, Floriana Campanile, Carmela Bonaccorso, Stefania Stefani, Stefania Stefani, Nicolò Musso, Dafne Bongiorno

Subject: Life Sciences, Microbiology Keywords: ESKAPE; heteroaryl-ethylenes; clinical strains; antimicrobial activity

Online: 13 May 2022 (03:30:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0070.v1

Taxonomic Re-Evaluation and Genomic Comparison of Novel Extracellular Electron Uptake-Capable Rhodovulum visakhapatnamense and Rhodovulum sulfidophilum isolates

Emily J Davenport, Arpita Bose

Subject: Life Sciences, Microbiology Keywords: phototrophic bacteria; phototrophic extracellular electron uptake; comparative genomics; transcriptomics; environmental microbiology

Online: 6 May 2022 (09:35:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0311.v1

Microbial Consortia and Mixed Plastic Waste: Pangenomic Analysis Reveals Potential for Degradation of Multiple Plastic Types via Previously Identified PET Degrading Bacteria

Sabrina Edwards, Rosa Leon-Zayas, Riyaz Ditter, Helen Laster, Grace Sheehan, Oliver Anderson, Toby Beattie, Jay L Mellies

Subject: Life Sciences, Microbiology Keywords: biodegradation; poly(ethylene)terephthalate (PET); low-density polyethylene (LDPE); plasticizers; mixed-plastics; pangenomes

Online: 30 April 2022 (04:22:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202204.0309.v1

Bacteriophages as Biocontrol Agents in Food Microbiology

Logan Gildea, Joseph Ayariga, Roberson Boakai K.

Subject: Life Sciences, Microbiology Keywords: bacteriophages; biocontrol; food production; bacterial resistance

Online: 29 April 2022 (15:49:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0298.v1

Intra-Species Genomic Variation in the Pine Pathogen Fusarium Circinatum

Mkhululi Maphosa, Emma T. Steenkamp, Aquillah Kanzi, Stephanie Van Wyk, Lieschen De Vos, Quentin C Santana, Tuan Duong, Brenda D Wingfield

Subject: Life Sciences, Microbiology Keywords: genome; accessory; core genome; Fusarium circinatum; structural variants; inversions; indels; pangenome

Online: 29 April 2022 (10:47:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0293.v1

Application of Two Field Methods for Monitoring Microbiological Water Quality in a Polluted Tropical Environment - A Pilot Study

Fabian Taube, Folke Cerenius, Karl Kristian Faksvåg, Hillary Limo, Amir Khorram-Manesh

Subject: Life Sciences, Microbiology Keywords: Austere; Healthcare; Microbiological; Military; Safety; Water

Online: 29 April 2022 (08:23:45 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0265.v1

Rhizosphere-Associated Microbiome Profile of Agriculture Reclaimed Lands in Egypt

Mohamed Hassan Korkar, Mahmoud Magdy, Samah Mohamed Rizk, Yosur Gamal Fiteha, Aiman Hanafy Atta, Mohamed Abdel-Salam Rashed

Subject: Life Sciences, Microbiology Keywords: Microbiome; Rhizosphere; Metabarcoding; 16S rDNA; Agriculture lands; Reclaimed lands

Online: 28 April 2022 (03:24:56 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0173.v1

Novel Mobile Integrons and Strain-Specific Integrase Genes within Shewanella spp. Unveil Multiple Lateral Genetic Transfer Events within the Genus

Teolincacihuatl Ayala Nuñez, Gabriela N. Cerbino, María Florencia Rapisardi, Cecilia Quiroga, Daniela Centrón

Subject: Life Sciences, Microbiology Keywords: Shewanella; chromosomal integron; mobile integron; integrase,gene cassette; lateral genetic transfer

Online: 19 April 2022 (03:52:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0114.v1

Elexacaftor-Tezacaftor-Ivacaftor As A Final Frontier in The Treatment of Cystic Fibrosis: Definition of The Clinical and Microbiological Implications in A Case-Control Study

Giuseppe Migliorisi, Mirella Collura, Francesca Ficili, Tiziana Pensabene, Dafne Bongiorno, Antonina Collura, Francesca Di Bernardo, Stefania Stefani

Subject: Life Sciences, Microbiology Keywords: Cystic Fibrosis; elexacaftor-tezacaftor-ivacaftor; microbiology; airway colonization

Online: 13 April 2022 (04:06:36 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0201.v2

Insights Into the Mechanisms of Lactobacillus acidophilus Activity Against Entamoeba histolytica by Using Thiol-Redox-Proteomics

Lotem Sarid, Eva Zanditenas, Jun Ye, Meirav Trebicz-Geffen, Serge Ankri

Subject: Life Sciences, Microbiology Keywords: Entamoeba histolytica; Lactobacillus acidophilus; probiotic; redoxomics; cysteine proteases

Online: 12 April 2022 (10:22:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0087.v1

Brown Dog Tick (Rhipicephalus sanguineus sensu lato) Infection with Endosymbiont and Human Pathogenic Rickettsia spp., Northern Mexico

Jordan Salomon, Nadia Angelica Fernandez Santos, Italo B Zecca, Jose G Estrada-Franco, Edward Davila, Gabriel L Hamer, Mario Alberto Rodriguez Perez, Sarah Hamer

Subject: Life Sciences, Microbiology Keywords: Rickettsia parkeri; Rhipicephalus sanguineus; dogs; tick-borne disease; rickettsiosis

Online: 11 April 2022 (03:37:16 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0029.v1

Variability of Genetic Characters Associated with Probiotic Functions in Lacticaseibacillus Species

Franca Rossi, Carmela Amadoro, Maria Pallotta, Giampaolo Colavita

Subject: Life Sciences, Microbiology Keywords: Lacticaseibacillus species; probiotic potential; genetic traits; presence in genomes

Online: 6 April 2022 (04:54:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202204.0015.v1

SARS-CoV-2 in Wastewater: Occurrence, Detection and Implications

Anshika Billa, Sanjeev Balda, Aarjoo Sharma, Rupali Dhingra, Shiv Nandan Sah, Neena Capalash, Prince Sharma

Subject: Life Sciences, Microbiology Keywords: Wastewater; Surveillance; SARS-CoV-2; Wastewater based epidemiology; COVID-19; Detection; Sewage

Online: 4 April 2022 (11:04:30 CEST)

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Preprint REVIEW | doi:10.20944/preprints202204.0004.v1

Zoliflodacin: A Hope to Treat Antibiotic-Resistant Neisseria gonorrhea

Mohammad Reza Mohammadi

Subject: Life Sciences, Microbiology Keywords: Neisseria gonorrhea; Zoliflodacin; Covid-19; Antibiotic-resistance; Treatment

Online: 1 April 2022 (07:26:28 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202204.0003.v1

Overexpression of a Membrane Transport System MSMEG_1381 and MSMEG_1382 Confers Multidrug Resistance in Mycobacterium smegmatis

Salini S, Balaji Muralikrishnan, Sinchana Bhat, R Ajay Kumar, Krishna Kurthkoti

Subject: Life Sciences, Microbiology Keywords: Drug resistance; Efflux pump; TetR transcriptional regulator; Mycobacteria; MmpL

Online: 1 April 2022 (07:22:46 CEST)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202111.0488.v2

Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids

Gency Gunasingh, Alexander Browning, Nikolas Haass

Subject: Life Sciences, Microbiology Keywords: spheroid; tumour spheroid; mounting; clearing; microscopy; structure; confocal

Online: 31 March 2022 (09:57:33 CEST)

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Preprint REVIEW | doi:10.20944/preprints202203.0376.v1

Immunity Escape of SARS-CoV-2 Variants: Omicron Leads the Way

Rupali Dhingra, Aarjoo Sharma, Sanjeev Balda, Anshika Billa, Shiv Nandan Sah, Neena Capalash, Prince Sharma

Subject: Life Sciences, Microbiology Keywords: SARS-CoV-2; Mutations; Omicron; Delta; Variants of Concern; Variants of Interest; Immunity Escape; Mechanisms

Online: 29 March 2022 (08:44:09 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202203.0367.v1

Cannabis sativa CBD Extract Shows Promising Antibacterial Activity against S. typhimurium and S. newington

Logan Gildea, Joseph Ayariga, Olufemi Ajayi, Junhaun Xu, Robert Villafane, Michelle Samuel-Foo

Subject: Life Sciences, Microbiology Keywords: Salmonella; Novel Antibacterial Agents; Cannabinoids

Online: 28 March 2022 (14:30:09 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202203.0348.v1

First Molecular Identification of Three Clinical Isolates of Fungi Causing Mucormycosis in Honduras

Bryan Ortiz, Isis Laínez-Arteaga, Celeste Galindo-Morales, Lilia Acevedo-Almendárez, Kateryn Aguilar, Diana Valladares, Miriam López, Gustavo Fontecha

Subject: Life Sciences, Microbiology Keywords: Mucormycosis; Honduras; Rhizopus oryzae; Apophysomyces ossiformis

Online: 25 March 2022 (15:24:39 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202203.0336.v1

Looking Forward: The Role of Academic Researchers in Building Sustainable Wastewater Surveillance Programs

Catherine Hoar, Jill McClary-Gutierrez, Aaron Bivins, Marlene Wolfe, Kyle Bibby, Andrea Silverman, Sandra McLellan

Subject: Life Sciences, Microbiology Keywords: Covid-19; SARS-CoV-2; Wastewater; research; surveillance

Online: 25 March 2022 (03:30:23 CET)

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Background: In the span of just two years, tracking the COVID-19 pandemic through wastewater surveillance has advanced from early reports of successful SARS-CoV-2 RNA detection in untreated wastewater to implementation of programs in at least 60 countries. Early wastewater monitoring efforts primarily originated in research laboratories and are now transitioning into more formal surveillance programs run in commercial and public health laboratories. A major challenge in this progression has been to simultaneously optimize methods and build scientific consensus while implementing surveillance programs, particularly during the rapidly changing landscape of the pandemic. Translating wastewater surveillance results for effective use by public health agencies also remains a key objective for the field. Objectives: We examine the evolution of wastewater surveillance to identify model collaborations and effective partnerships that have created rapid and sustained success. We propose needed areas of research and key roles academic researchers can play in the framework of wastewater surveillance to aid in the transition of early monitoring efforts to more formalized programs within the public health system. Discussion: Wastewater surveillance has rapidly developed as a public health tool Clinical testing programs are ramping down and home testing is on the rise, making wastewater monitoring important for future surveillance of COVID-19. Our experience in initiating and implementing wastewater surveillance programs in the United States has allowed us to reflect on key barriers and organizational challenges and draw useful lessons. As wastewater surveillance programs are formalized, the working relationships developed between academic researchers, commercial and public health laboratories, and data users should continue and should promote knowledge co-development. While wastewater surveillance has demonstrated utility for tracking COVID-19, there remain technical challenges and open scientific questions that researchers are equipped to address, which will contribute to building robust surveillance programs that provide public health practitioners with new insights into population health.

Preprint ARTICLE | doi:10.20944/preprints202203.0267.v1

Bacteria-Derived Protein Aggregates Contribute to the Disruption of Host Proteostasis

Alyssa C. Walker, Rohan Bhargava, Autumn S. Dove, Amanda S. Brust, Ali A. Owji, Daniel M. Czyz

Subject: Life Sciences, Microbiology Keywords: Caenorhabditis elegans; proteostasis; bacteria; neurodegenerative diseases; protein aggregates; protein conformational disease; butyrate; aminoglycoside; Pseudomonas aeruginosa

Online: 18 March 2022 (09:21:45 CET)

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Preprint REVIEW | doi:10.20944/preprints202203.0262.v1

Augmenting Azoles with Drug Synergy to Expand The Antifungal Toolbox

Aidan Kane, Dee A. Carter

Subject: Life Sciences, Microbiology Keywords: Keywords: antifungal; azole; synergy; mycosis; resistance; Candida; dermatophytes; natural products

Online: 18 March 2022 (07:04:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0201.v1

Insights Into the Mechanisms of Lactobacillus acidophilus Activity Against Entamoeba histolytica by Using Thiol-Redox-Proteomics

Lotem Sarid, Eva Zanditenas, Jun Ye, Meirav Trebicz-Geffen, Serge Ankri

Subject: Life Sciences, Microbiology Keywords: Entamoeba histolytica; Lactobacillus acidophilus; probiotic; redoxomics; cysteine proteases

Online: 15 March 2022 (10:38:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0108.v1

Intestinal Parasitic Infections among the Pediatric Patients in a Metropolitan City of Bangladesh with Emphasis on Cryptosporidiosis

Nusrat Jahan Nipa, Nasima Akter, Hasina Momotaj Hira, Farhana Akter, Dilshad Jahan, Salequl Islam, Ayukafangha Etando, Kona Chowdhury, Rahnuma Ahmad, Mainul Haque

Subject: Life Sciences, Microbiology Keywords: intestinal parasitic; contagions; diseases; pediatric patients; Metropolitan City of Bangladesh; cryptosporidiosis

Online: 7 March 2022 (15:51:25 CET)

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Preprint ARTICLE | doi:10.20944/preprints202203.0005.v1

Helicobacter Pylori in Native Americans in Northern Arizona

Fernando P Monroy, Heidi E. Brown, Priscilla R. Sanderson, Gregory Jarrin, Mimi Mbegbu, Shari Kyman, Robin H Harris

Subject: Life Sciences, Microbiology Keywords: Helicobacter pylori; gastric cancer; Navajos; Native Americans; American Indians; virulence factors

Online: 1 March 2022 (06:52:14 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0321.v1

Enteroinvasive Escherichia Coli is Inefficient to Escape from Autophagy during Caco-2 Cellular Infection

Silvia Yumi Bando, Ana Carolina Ramos Moreno, Hadassa Cristhina de Azevedo Soares dos Santos, Fernanda Bernardi Bertonha, Lucas Gonçalves Ferreira, Marina Baquerizo Martinez, Carlos Alberto Moreira-Filho

Subject: Life Sciences, Microbiology Keywords: Enteroinvasive Escherichia coli; Shigella flexneri; Caco-2 cells; icsB; autophagy; WGCNA; gene co-expression network

Online: 25 February 2022 (03:16:26 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0286.v1

Assay for Evaluating the Abundance of Vibrio Cholerae and Its O1 Serogroup Subpopulation Directly from Water Without DNA Extraction

Tania Nasreen, Nora A.S. Hussain, Jia Yee Ho, Vanessa Zie Jie Aw, Munirul Alam, Stephanie K Yanow, Yann Felix Boucher

Subject: Life Sciences, Microbiology Keywords: cholera; Vibrio cholerae; Vibrio cholerae O1; endemic; toxigenic; abundance and qPCR

Online: 23 February 2022 (06:45:35 CET)

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Preprint REVIEW | doi:10.20944/preprints202202.0248.v1

Lactobacilli Infection Case Reports in the Last Three Years and Safety Implications

Franca Rossi, Carmela Amadoro, Giampaolo Colavita

Subject: Life Sciences, Microbiology Keywords: lactobacilli infections; update; case reports; virulence traits; safety implications

Online: 21 February 2022 (08:54:37 CET)

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Preprint REVIEW | doi:10.20944/preprints202202.0200.v1

Review and Comparison of Antimicrobial Resistance Gene Databases

Márton Papp, Norbert Solymosi

Subject: Life Sciences, Microbiology Keywords: antibiotic resistance genes; antibiotic resistance gene database; annotation of antibiotic resistance genes

Online: 17 February 2022 (04:52:10 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0189.v1

Genomic Insights on the Functional Capabilities of the Cyano-Sphere of Edible Andean Nostoc Macrocolonies (Llayta)

Claudia Vilo, Alexandra Galetovic, Qunfeng Dong, Benito Gómez-Silva

Subject: Life Sciences, Microbiology Keywords: cyanosphere; cyanobacteria; Llayta; Nostoc macrocolonies; metagenomics; microbiome

Online: 15 February 2022 (20:25:40 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0166.v1

Characterization of Bifidobacterium asteroides Isolates

Alessandra Pino, Bachir Benkaddour, Rosanna Inturri, Pietro Amico, Susanna Vaccaro, Nunziatina Russo, Amanda Vaccalluzzo, Gianluigi Agolino, Cinzia Caggia, Cinzia L. Randazzo

Subject: Life Sciences, Microbiology Keywords: microbiological characterization; safety; VanZ; isolation; vancomycin resistant gene; genome; bee; honey

Online: 11 February 2022 (21:17:45 CET)

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Preprint ARTICLE | doi:10.20944/preprints202202.0003.v1

Potent Activity of High Concentration Ozone Therapy Against Antibiotic-Resistant Bacteria

Karyne Rangel, Fellipe O Cabral, Guilherme Curty Lechuga, João P.R.S. Carvalho, Maria H.S. Villas-Bôas, Vitor Midlej, Salvatore Giovanni De-Simone

Subject: Life Sciences, Microbiology Keywords: ozone; detrimental effect; pathogenic bacteria; antimicrobial resistance; SEM; ESKAPE pathogens

Online: 1 February 2022 (11:02:26 CET)

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Preprint REVIEW | doi:10.20944/preprints202201.0320.v1

Biochemical and Metabolical Pathways Associated with Microbiota-Derived Butyrate in Colorectal Cancer and Omega-3 Fatty Acids Implications: A Narrative Review

Adelina Silvana Dragomir, Șerban Mircea Negru, Mădălina Preda, Raluca Ioana Mihăilă, Isabela Anda Komporaly, Elena Adriana Mateianu, Cristian Virgil Lungulescu, Lidia Anca Kajanto, Bogdan Georgescu, Emanuel Alin Radu, Dana Lucia Stănculeanu

Subject: Life Sciences, Microbiology Keywords: Butyrate; Colorectal cancer; Gut microbiota; Diet; omega-3 PUFAs

Online: 21 January 2022 (11:33:23 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0225.v2

Misdiagnosis of Bacterial Pathogens by the Diagnostic Centers: A Potential Route for Antibiotic Resistance

Shanaz Fatema Bristy, Md Reaz Uddin, Bushra Ayat Meghla, Taslima Akter Tisha, Md. Aftab Uddin, Mohammad Tarequl Islam, Masayasu Mie, Eiry Kobatake, Mohib Ullah Khondoker, Taslin Jahan Mou, Md. Ahsanul Haq, Md. Fokhrul Islam, Zinia Islam, Sharif Hossain, Nafisa Azmuda, Mohd. Raeed Jamiruddin, Mainul Haque, Nihad Adnan

Subject: Life Sciences, Microbiology Keywords: Misdiagnosis; Random Amplification of Polymorphic DNA; 16S rRNA Sequencing; Loop-Mediated Isothermal Amplification; Antibiotic Resistance

Online: 19 January 2022 (11:39:41 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0263.v1

Novel ABC Transporter Associated with Fluconazole Resistance in Aging of Cryptococcus neoformans

Natalia Kronbauer Oliveira, Somanon Bhattacharya, Rina Gambhir, Manav Joshi, Bettina C Fries

Subject: Life Sciences, Microbiology Keywords: drug resistance; ABC transporter; efflux pump; cryptococcosis; aging

Online: 18 January 2022 (15:56:02 CET)

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Preprint REVIEW | doi:10.20944/preprints202201.0255.v1

Invasive Trichosporonosis in Neonates and Pediatric Patients with Malignancies or Hematologic Disorders

Maria Kourti, Emmanuel Roilides

Subject: Life Sciences, Microbiology Keywords: Trichosporon; trichosporonosis; neonate; hematologic disorder; malignancy

Online: 18 January 2022 (12:35:06 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0234.v1

Antimicrobial Resistance Surveillance System Mapping in Different Countries

Ramendra Pati Pandey, Riya Mukherjee, Chung-Ming Chang

Subject: Life Sciences, Microbiology Keywords: AMR, Surveillance; One Health Approach; Alternative Antibiotics; Comparative Medicine; Phage Therapy

Online: 17 January 2022 (14:46:22 CET)

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Antibiotics are in excessive use that has extensively increased antimicrobial resistance worldwide which has become the major public concern among the countries. To control this threat proper monitoring of the antimicrobial usage along with the increasing rate of antimicrobial resistance (AMR) is required. Further, surveillance of both the parameters is highly recommended for comparing the differences in distinct countries. Moreover, alternatives for antibiotics are also surveyed and are being researched for quick use in the near future. AMR is an issue that needs immense attention from various sectors. Thus, intervention of multisector is highly encouraged for better outcomes. One Health is one of the approaches that play a vital role in resolving this issue. In this research paper, six different European countries are discussed in terms of antimicrobial usage and AMR in the human and livestock sectors with the help of literature study and various reports published by different organizations. Data study has been conducted to collect the data for comparison study. Data sources of AMR and antimicrobial usage are analyzed and a thorough comparison of both antimicrobial use and AMR are conducted. Also, the application of One Health is studied for a balanced system. This article provides about various surveillance systems that are formed only to keep a track on the upcoming situation of AMR and the consumption of antimicrobials by the humans as well as animals. The article does not provide about all the details required to monitor the AMR issue but firmly allow the readers to get acknowledged with the broad information about the antimicrobial resistance across the six countries of Europe. The regular data collected by the different organizations play a vital role in monitoring the status of AMR and antimicrobial usage by humans and in live stocks. These annual reports have highly helped the government to decide for alternatives and have focused in many training activities to combat the AMR situation globally. AMR prevention is linked to the One Health concept. As antibiotic resistance genes persist on an interface between environment and animal and animal health, an approach is required in all three areas that stress the concept of 'One Approach to Health.'

Preprint ARTICLE | doi:10.20944/preprints202201.0181.v1

One Health Approach for combating Antimicrobial Resistance in Animals

Chung-Ming Chang, Ramendra Pati Pandey, Riya Mukherjee

Subject: Life Sciences, Microbiology Keywords: One Health Strategies; Antimicrobial Resistance; Salmonella isolates; Poultry Farms, Turkey Poults

Online: 12 January 2022 (18:12:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0156.v1

Comprehensive analysis of imipenemase (IMP)-type metallo-Beta-lactamase showing global distribution threating Asia

Pisut Pongchaikul, Paninee Mongkolsuk

Subject: Life Sciences, Microbiology Keywords: beta-lactamase; carbapenemase; antimicrobial resistance

Online: 12 January 2022 (00:10:49 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0045.v1

Regulation of Gene Expression of phiEco32-like Bacteriophage 7-11

Daria Lavysh, Vladimir Mekler, Evgeny Klimuk, Konstantin Severinov

Subject: Life Sciences, Microbiology Keywords: bacteriophage; alternative sigma factor; transcription regulation

Online: 5 January 2022 (15:43:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints202201.0037.v1

Broad-spectrum Antimicrobial ZnMintPc Encapsulated in Magnetic-nanocomposites with Graphene Oxide/MWCNTs Based on Bimodal Action of Photodynamic and Photothermal Effect

Coralia Fabiola Cuadrado, Antonio Díaz-Barrios, Kleber Orlando Campana, Eric Cardona Romani, Francisco Javier Quiroz, Stefania Nardecchia, Alexis Debut, Karla Vizuete, Dario Niebieskikwiat, Camilo Ernesto Ávila, Mateo Alejandro Salazar, Cristina Cecibel Garzón-Romero, Ailín Amira Blasco-Zúñiga, Miryan Rosita Rivera, María Paulina Romero

Subject: Life Sciences, Microbiology Keywords: Antimicrobial nanomaterials; Carbon Nanotubes; Graphene; Magnetic Nanoparticles; hydrogel; Photodynamic Therapy; Photothermal Therapy; Nanocarrier

Online: 5 January 2022 (12:02:34 CET)

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Preprint REVIEW | doi:10.20944/preprints202112.0433.v1

Anti-cancer Activity of Human Gastrointestinal Bacteria

Sameer Quazi

Subject: Life Sciences, Microbiology Keywords: Lactobacillus spp, Bacillus spp., Anti-cancer, Probiotics, Gastrointestinal, Dysbiosis.

Online: 27 December 2021 (14:50:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0342.v1

Airborne Alt a 1 Dynamic and Its Relationship With the Airborne Dynamics of Alternaria Conidia and Pleosporales Spores

Concepción De Linares, David Navarro, Rut Puigdemunt, Jordina Belmonte

Subject: Life Sciences, Microbiology Keywords: Allergens; Alt a 1; Alternaria; Pleosporales; fungal spores; aerobiological samplers.

Online: 21 December 2021 (13:55:09 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0283.v1

Snapshot of Mycobacterium tuberculosis Phylogenetics from an Indian State of Arunachal Pradesh Bordering China

Rashmi S Mudliar, Umay Kulsum, Syed Beenish Rufai, Mika Umpo, Moi Nyori, Sarman Singh

Subject: Life Sciences, Microbiology Keywords: DST; MGIT-960; WGS; cgMLST; SNP barcoding; phylogeny

Online: 17 December 2021 (08:20:58 CET)

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Preprint ARTICLE | doi:10.20944/preprints202112.0024.v1

New Strategies for Efficient Expression of Heterologous Sugar Transporters in Saccharomyces cerevisiae

Marilia M. Knychala, Angela A. dos Santos, Leonardo G. Kretzer, Fernanda Gelsleichter, Maria José Leandro, César Fonseca, Boris U. Stambuk

Subject: Life Sciences, Microbiology Keywords: xylose; hxt-null; ubiquitinylation; lysine; truncated permease; endocytosis; XUT1; ROD1, ROG3

Online: 2 December 2021 (09:54:07 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0560.v1

Extended ɛ34 Phage TSP Renatures After Urea-acid Unfolding

Joseph Ayariga, Logan Gildea, Robert Villafane

Subject: Life Sciences, Microbiology Keywords: Renaturation; Denaturation; Phage; Fusion protein; Podoviruses

Online: 30 November 2021 (11:35:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0558.v1

Peptidoglycan-Free Bacterial Ghosts Confer Enhanced Protection against Yersinia Pestis Infection

Svetlana V. Dentovskaya, Anastasia S. Vagaiskaya, Mikhail E. Platonov, Alexandra S. Trunyakova, Sergei A. Kotov, Ekaterina A. Krasil'nikova, Galina M. Titareva, Elizaveta M. Mazurina, Tat'yana V. Gapel'chenkova, Rima Z. Shaikhutdinova, Sergei A. Ivanov, Tat'yana I. Kombarova, Vladimir N. Gerasimov, Vladimir N. Uversky, Andrey P. Anisimov

Subject: Life Sciences, Microbiology Keywords: Yersinia pestis; vaccine; guinea pigs; bubonic plague; inactivated vaccine; phage; bacterial ghost; protection; protein-E-mediated lysis, holin-endolysin system

Online: 30 November 2021 (11:08:18 CET)

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Preprint TECHNICAL NOTE | doi:10.20944/preprints202111.0488.v1

Rapid Optical Clearing for High-Throughput Analysis of Tumour Spheroids

Gency Gunasingh, Alexander Browning, Nikolas Haass

Subject: Life Sciences, Microbiology Keywords: spheroid; tumour spheroid; mounting; clearing; microscopy; structure; confocal

Online: 25 November 2021 (16:15:34 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0474.v1

Effects of a Direct-Fed Microbial and Feedlot Pen Environmental Change on Phenotypic Resistance among Enterococci Isolated from Beef Cattle Feces

Sarah A. Murray, Ashlyn C. Holbert, Keri N. Norman, Sara D. Lawhon, Jason E. Sawyer, Harvey Morgan Scott

Subject: Life Sciences, Microbiology Keywords: probiotic; Enterococcus faecium; antimicrobial resistance; environmental change

Online: 25 November 2021 (12:49:29 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0426.v1

Reoccurring Bovine Anthrax in Germany on the Same Pasture after 12 Years

Peter Braun, Wolfgang Beyer, Matthias Hanczaruk, Julia Riehm, Markus Antwerpen, Christian Otterbein, Jacqueline Oesterheld, Gregor Grass

Subject: Life Sciences, Microbiology Keywords: Bacillus anthracis; anthrax; outbreak; phylogenetics; detection assay

Online: 23 November 2021 (14:44:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0376.v1

Mast Cell Response to Leishmania mexicana and Sand Fly Salivary Proteins is Modulated by Androgens

Laura Sánchez-García, Armando Perez-Torres, Samira Muñoz-Cruz, Jorge Morales-Montor, Ingeborg Becker

Subject: Life Sciences, Microbiology Keywords: Mast cells; Leishmania mexicana; sand fly salivary proteins; sexual hormones

Online: 22 November 2021 (10:51:24 CET)

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Preprint SHORT NOTE | doi:10.20944/preprints202111.0374.v1

Do It Yourself! – Experiences with Self-Synthesized CsTFA for RNA-SIP Analyses

Markus Egert, Severin Weis, Magnus S. Schmidt

Subject: Life Sciences, Microbiology Keywords: Stable Isotope Probing; RNA; density gradient; ultracentrifugation; microbial ecology

Online: 22 November 2021 (10:41:51 CET)

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Preprint REVIEW | doi:10.20944/preprints202111.0358.v1

Nanotechnological Advancement in Antimicrobial Drug Designing

Mohammad Oves, Mohd Ahmar Rauf

Subject: Life Sciences, Microbiology Keywords: multidrug-resistant (MDR); Nanotechnology; Antimicrobial

Online: 19 November 2021 (14:31:33 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0347.v1

Inactivation of MrPigH in Monascus ruber M7 Results in its Monascus Pigments Increase and Citrinin Decrease with mrpyrG Selection Marker

Li Li, Na Xu, Fusheng Chen

Subject: Life Sciences, Microbiology Keywords: Monascus ruber; mrpigH; Monascus pigments; citrinin

Online: 19 November 2021 (11:29:21 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0287.v1

Benzaldehyde Attenuates the Fifth Stage Larval Excretory-Secretory Product of Angiostrongylus cantonensis-Induced Injury in Mouse Astrocytes via Regulation of Endoplasmic Reticulum Stress and Oxidative Stress

Kuang-Yao Chen, Yi-Ju Chen, Chien-Ju Cheng, Kai-Yuan Jhan, and Lian-Chen Wang

Subject: Life Sciences, Microbiology Keywords: Excretory-secretory products; Angiostrongylus cantonensis; Astrocytes; Benzaldehydes; endoplasmic reticulum stress; oxidative stress

Online: 16 November 2021 (11:34:13 CET)

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Preprint CASE REPORT | doi:10.20944/preprints202111.0284.v1

Orientia tsutsugamushi and Epstein-Barr Virus in a Young Adult with Acalculous Cholecystitis: A Case Report and Epidemiology of Scrub Typhus in the Maldives

Hisham Ahmed Imad, Aishath Azna Ali, Mariyam Nahuza, Rajan Gurung, Abdulla Ubaid, Aishath Maeesha, Sariu Ali Didi, Rajib Kumar Dey, Abdullah Isneen Hilmy, Aishath Hareera, Ibrahim Afzal, Wasin Matsee, Wang Nguitragool, Emi E. Nakayama, Tatsuo Shioda

Subject: Life Sciences, Microbiology Keywords: acute acalculous cholecystitis; Orientia tsutsgugamushi; scrub typhus; eschar; Epstein-Barr virus; re-activation; clinical manifestation; Maldives

Online: 16 November 2021 (09:34:22 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0212.v1

Survey of Freshly-Harvested Oat Grains from Southern Brazil Reveals High Incidence of Type B Trichothecenes and Associated Fusarium Species

Mariana Pinheiro, Caio Henrique Tadashi Iwase, Elem Caramês, BRUNO G. BERTOZZI, Lorena Carnielli-Queiroz, Nadia C. Lângaro, Eliana B. Furlong, Benedito Correa, Liliana O. Rocha

Subject: Life Sciences, Microbiology Keywords: oats; fusarium sambucinum species complex; deoxynivalenol; nivalenol; mycotoxin

Online: 11 November 2021 (13:05:00 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0176.v1

Mobile Colistin Resistance Genetic Determinants of Non-typhoid Salmonella enterica Isolates from Russia

K. V. Kuleshov, A. S. Pavlova, E. D. Shedko, Y. V. Mikhaylova, G. Margos, S. Hepner, I. V. Chebotar, E. V. Korneenko, A. T. Podkolzin, V. G. Akimkin

Subject: Life Sciences, Microbiology Keywords: whole genome sequencing; antibiotic resistance; Salmonella Enteritidis; Salmonella Typhimurium; Salmonella Bovismorbificans; colistin resistance; mcr-1; mcr-9

Online: 9 November 2021 (13:46:05 CET)

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Preprint REVIEW | doi:10.20944/preprints202111.0157.v1

Healthy Cattle Microbiome and Dysbiosis in Diseased Phenotypes

Alaa Khalil, Aamina Batool, Sania Arif

Subject: Life Sciences, Microbiology Keywords: Microbiome; Cattle; Johne’s disease; Dysbiosis; Mastitis

Online: 8 November 2021 (15:17:16 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0058.v1

Detrimental Effect of Ozone on Pathogenic Bacteria

Karyne Rangel, Felipe O Cabral, Guilherme Curty Lechuga, João P.R.S. Carvalho, Maria H.S. Villas-Bôas, Victor Midlej, Salvatore Giovanni De-Simone

Subject: Life Sciences, Microbiology Keywords: ozone; detrimental effect; pathogenic bacteria; antimicrobial resistance; ROS; Acinetobacter baumannii; Pseudomonas aeruginosa

Online: 3 November 2021 (07:55:57 CET)

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Preprint COMMUNICATION | doi:10.20944/preprints202111.0055.v1

The Effect of Natural Falling and Dipping of House Fly (Musca domestica) on the Microbial Contamination of Water and Milk: A Short Communication Report

Nabih Abdulrahman Baeshen, Nagwa Thabet Elsharawy, Naseebh Nabih Baeshen, Mohammed Nabih Baeshen

Subject: Life Sciences, Microbiology Keywords: hosue fly,; falling,; dipping,; antimicrobial,; milk,; water

Online: 2 November 2021 (22:48:42 CET)

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Preprint ARTICLE | doi:10.20944/preprints202111.0014.v1

Dietary Exposure and Risk Assessment of Aflatoxin M1 for Children Aged 1 to 9 Years Old in Serbia

Dragan R Milićević, Jelena Milešević, Mirjana Gurinovic, Saša Janković, Jasna Đinović, Milica Zekovic, Maria Glibetic

Subject: Life Sciences, Microbiology Keywords: aflatoxin M1; milk; dairy products; risk assessment; children

Online: 1 November 2021 (12:26:43 CET)

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