Abstract: Chronic allograft dysfunction (CLAD) is the main cause of graft loss after lung transplantation (LTR). Within the immunological factors involved in CLAD development, the antibody-mediated rejection (ABMR) has the most impact. However, ABMR diagnosis is difficult due to the limited sensitivity of histopathological, immunhistochemical, and immunological criteria currently used. Growing evidence is demonstrating the impact of molecular mismatch in ABMR; here, we ought to assess the potential role of molecular mismatch in CLAD development. A total of 457 LTR were recruited for the study, with HLA type from donors and recipients to assess molecular mismatch, and with a minimum follow-up of 180 days. The combination of molecular mismatch in class-II (HLA-EMMA and HLA-Matchmaker algorithms) with EMMA DR score >12 and antibody verified eplet mismatch in DRB1345 (AbV DRB1345) > 3 predicts CLAD development independently of ex-smoker, prolonged period of hospitalization (>33 days), acute cellular rejection (ACR), and ABMR. The HR of the prediction model for molecular mismatch in class-II was 1.52 (1.01-2.56, p=0.045). This observation could point to a potential role of poor molecular mismatch in class-II to fill the gap of underdiagnosis of ABMR, previous to CLAD development. Prospective studies should be addressed to confirm the utility of molecular mismatch in the identification of patients at risk of CLAD development.

Abstract: The management of asymptomatic bacteriuria (ASB) and candiduria (ASC) in kidney transplant recipients during the early post-transplant period is controversial. This study aimed to evaluate whether treating, versus not treating, ASB and ASC episodes in the first two months after kidney transplantation influences clinical outcomes and the emergence of multidrug resistant (MDR) infections. We conducted a single-center retrospective cohort study enrolling patients with ASB or ASC occurring in two first two month after kidney transplantation between January 2019 and July 2024. Patients were classified into treated and untreated groups. The primary endpoint was 30-day mortality. Secondary endpoints included mortality at 90, 180 and 360 days; incidence of sepsis or septic shock; bacteremia/candidemia, hospi-talization, graft loss; decline in renal function, urinary tract infections (UTIs), recur-rent UTI and rate of MDR colonization/infection. We enrolled 59 kidney transplant recipients and observed 147 episodes of ASB/ASC. Of the 147 episodes, 95 were untreated and 52 were treated. No significant differences were observed between treated and untreated patients in 30-day (2.1% vs. 3.8%) or 90-day mortality (2.1% vs. 1.9%), nor in any of the secondary clinical outcomes. How-ever, patients who received treatment tended to have a higher rate of MDR coloniza-tion/infection (63% vs. 46%). MDR pathogen isolation was significantly associated with increased risks of septic shock (OR 4.639,p=0.04), bacteremia/candidemia (OR 3.734,p=0.01), hospitalization (OR 2.183,p=0.03), and renal function deterioration (OR 3.93,p=0.03). Antimicrobial treatment of ASB and ASC in the early post-transplant period would seem not to confer clinical benefit and may increase the risk of MDR coloniza-tion/infection.

Abstract: Kidney transplantation activity at the University Hospital of Guadeloupe was briefly interrupted at the onset of the COVID-19 pandemic. This study assessed patient and graft survival in 335 recipients transplanted between 2013 and 2023, comparing those transplanted before 2020 and after the resumption of activity. Most patients (70%) received transplants before 2020, with 30% after-ward. ABO compatibility was observed in 98.2% of grafts from brain-dead do-nors. Peak transplant activity occurred in 2018, followed by a decline until 2021 and recovery from 2022. After 2020, recipients were more likely to be el-derly (≥70 years), immunized, obese, have heterozygous sickle cell disease, or polycystic kidney disease (p < 0.05). Mean cold ischemia time decreased (p = 0.009), while warm ischemia time increased (p < 0.001). Graft survival re-mained stable, with 97.5% at 6 months and 89.8% at 4 years for transplants before 2020, versus 100% and 96.9%, respectively, after 2020 (p = 0.160). Patient survival did not differ between periods (p = 0.199). Independent factors associ-ated with mortality included recipient age ≥ 60 years, diabetes, graft failure, transplantation before 2020, cold ischemia time ≥ 1200 minutes, and graft py-elonephritis. Despite an increased proportion of transplants with expanded criteria after 2020, graft and patient survival were not adversely affected.

Abstract: Background: Chyle leakage is known to be a rare postoperative complication following liver transplantation (LT), and continuously leaked large quantities of chyle leakage can worsen prognosis. However, there is no explicit understanding of its mechanism and no existing reports showing the influence of chyle leakage after LT on blood concentration of the drug tacrolimus. Case presentation: Despite escalating tacrolimus daily dose to 4.0 mg with CYP3A5 inhibitor, blood concentrations remained subtherapeutic (1.7–2.5 ng/mL) during active leakage. Conservative treatments failed, prompting intraperitoneal injection of erythromycin (0.75 g)-25% glucose solution (40 mL every other day). Following three treatments, chylous drainage diminished significantly, and tacrolimus concentrations abruptly increased to 14.7 ng/mL 2 days after leakage resolved. Dose adjustments subsequently stabilized levels at 4.6–6.2 ng/mL with daily dose of 2.0mg. Conclusions: Intraperitoneal injection of erythromycin hypertonic solution may promote lymphatic fistula closure via chemical stimulation. Chyle leakage may reduce tacrolimus blood concentration through drug loss in chylous fluid. This case highlights the need for clinical attention to the association between chyle leakage and immunosuppressant concentrations, though further studies are required for validation.

Abstract: Polyomavirus-associated nephropathy was first reported more than 50 years ago, however it still represents a cause of renal injury, with occurrence rates of 1%-10%, particularly in the firs two years following kidney transplantation. The role played by immunesuppression in viral reactivation is well aknowledged and the modulation of immunesuppression level is the main strategy for the management. Viral and immu-nological evaluation are fundamental for optimizing the diagnostic and clini-cal-therapeutic pathway. In this review, main features of polyomavirus BK and asso-ciated nephropathy will be addressed from a virological point of view.

Abstract: Background: The accurate evaluation of anti-ABO antibodies is essential for risk stratification in ABO-incompatible (ABOi) transplantation. Historically, hemaggluti-nation-based titration has been the cornerstone of such an assessment; however, dif-ferent tools are being evaluated in this context. In recent years, several novel methods have been reported. Methods: A narrative review was conducted using PubMed, Scopus, and Google Scholar, focusing on recent studies evaluating anti-ABO antibody measurement tech-niques in the context of ABOi organ transplantation. Results: In addition to the conventional tube method, techniques such as column ag-glutination technology, flow cytometry, and enzyme-linked immunosorbent assay are utilized for anti-ABO antibody assessment. However, any particular technique, sig-nificant interinstitutional and interoperator variabilities have been reported due to differences in the detailed protocols and the inherently subjective nature of some techniques. Moreover, these assays are based on the antibody binding to ABO anti-gens expressed on red blood cells, which might not accurately reflect the clinical con-text of organ transplantation. In recent years, technological advances have enabled the development of novel assays evaluating antibody responses specifically against the ABO antigens expressed on vascular endothelial cells. These include glycan mi-croarrays, which differentiate responses by ABO antigen subtypes, and CD31-based microarrays, wherein recombinant CD31 proteins expressing ABO antigens are im-mobilized. These approaches are applied to assess clinically relevant anti-ABO anti-bodies in the context of ABOi organ transplantation. Conclusions: The objective evaluation of antibody titers against ABO antigens on vascular endothelial cells might not only enable a more accurate risk assessment but also facilitate meaningful comparisons between institutions.

Abstract: Selecting donor organs is both a science and an art. The calculus is often complex evaluating recipients’ acute needs and the quality and risks of the donor organs. Sarcoidosis is usually a disease of exclusion and considered an idiopathic inflammatory granulomatous process frequently in the lung but often with multiorgan involvement. New insights into the interplay between the environmental or occupational antigens and the susceptible genetic host in sarcoidosis have come to light. One such occupational antigen with mixed data regarding the risk of developing sarcoidosis after exposure is silica dust. Despite strict exposure limitations enforced in the United States and several other governing bodies worldwide, clusters of silicosis are on the rise due to engineered stone (quartz), which contain silica particles that when cut can create a dust that can exceed exposure limitations. Data on the quartz-sarcoidosis relationship is minimal, and thus questions exist regarding the risk of silica dust exposure and the risk of sarcoidosis. Silicosis tends to only affect the lungs, preventing them usually from being transplanted while sarcoidosis is recognized as having a high probability of multiple organ involvement. An index case discovered during organ recovery highlights the possible association between silica exposure and sarcoidosis. This case also examples the complexity of donor organ evaluation and the challenges in organ procurement from exposed individuals.

Abstract: BK virus-associated hemorrhagic cystitis (BKV-HC) is a significant complication following alloge-neic hematopoietic cell transplantation (HCT), typically resulting from the reactivation of latent BK polyomavirus in the context of profound immunosuppression. This review addresses three core re-search questions: (1) What are the most accurate and practical diagnostic strategies for identifying BKV-HC in HCT recipients? (2) Which therapeutic interventions demonstrate the greatest efficacy and safety in clinical practice? (3) How can emerging immunological insights inform future indi-vidualized treatment approaches? Current diagnostic approaches rely on clinical assessment combined with quantitative PCR to detect active viral replication in urine or plasma, with exclusion of alternative causes of hematuria and renal injury. BKV-HC presents with a wide spectrum of clinical severity and contributes substan-tially to morbidity, prolonged hospitalization, and healthcare resource utilization. While reduction of immunosuppression remains the primary intervention, antiviral agents such as cidofovir, leflunomide, and fluoroquinolones have shown inconsistent benefits. Supportive strategies, in-cluding bladder irrigation, and novel immunotherapies targeting virus-specific immune responses are under active investigation. Given the disease’s complex pathogenesis and the absence of standardized treatment protocols, future research should prioritize prospective trials to establish validated viral load thresholds, op-timize therapeutic algorithms, and explore the utility of immune monitoring to inform personalized management.

Abstract: The reactivation of BK polyomavirus (BKPyV) during severe immunosuppression plays a crucial role in two significant syndromes observed in transplant recipients: BK polyomavirus-associated nephropathy (BKPyVAN) in kidney transplant patients and BK polyomavirus-associated hemorrhagic cystitis (BKPyV-HC) in hematopoietic cell transplant (HCT) recipients. This review aims to summarize current understanding and lingering ambiguity by looking at three primary questions: (1) In cases with BKPyV-related illnesses in transplant patients, which diagnostic methods have the best track record of accuracy and success? (2) Which therapy approaches have the best track records of safety and efficacy in real-world clinical settings? (3) What can immunological research teach us about the development of future tailored treatments? Diagnosis involves the patient's appearance, ruling out other potential causes, and employing quantitative PCR to identify active viral replication in urine or plasma. BKPyV-HC can vary from self-limited hematuria to potentially fatal bleeding, while BKPyVAN may lead to loss and dysfunction of the allograft. Reducing immunosuppression remains the key aspect of treatment. However, the effectiveness of antivirals (such cidofovir and leflunomide) is not always the same, and supporting measures depend on the syndrome. Researchers are looking into new immunotherapies, such as virus-specific cytotoxic T cells. Due to the intricate viro-immunopathology and lack of defined treatment regimens, future initiatives should focus on prospective studies to establish validated thresholds, enhance management algorithms, and integrate immune surveillance into individualized therapy.

Abstract: Background/Objectives: The extended-release once-daily tacrolimus (LCP-Tac) formulation demonstrates enhanced bioavailability and a sustained pharmacokinetic profile compared to the immediate-release twice-daily tacrolimus (IR-Tac) formulation. Switching from IR-Tac to LCP-Tac is emerging as a viable immunosuppressive strategy in clinical settings. This study aimed to examine the influence of genetics and other covariates on the general conversion ratio of 1:0.7. Methods: A population pharmacokinetic model was developed using rich sampling data from 30 stable renal transplant patients who received both IR-TAC and LCP-TAC formulations. Results: The pharmacokinetic (PK) profile was best described by a two-compartment model with first-order absorption and linear elimination for both formulations. The model effectively captured differences in the absorption phases of the two formulations by estimating distinct absorption rate constants and lag times. It also accounted for circadian rhythm effects on apparent elimination clearance and absorption rate constants for IR-Tac. A key finding was the significant impact of the CYP3A5 genetic polymorphism on apparent clearance and the dose conversion rate from IR-Tac to LCP-Tac. CYP3A5*1 expressers had higher clearance and achieved lower exposure with the same IR-Tac dose regimen compared to non-expressers. Conclusion: The enhanced oral bioavailability of LCP-Tac necessitates dose reductions across the patient cohort following conversion from IR-Tac. However, CYP3A5*1 expressers require a more pronounced reduction (1:0.6 conversion ratio) compared to non-expressers (1:0.7) to maintain steady-state concentrations achieved initially with IR-Tac.

Abstract: In renal transplant recipients (RTRs), kidney graft failure and cardiovascular disease are prevalent and associated with morbidity and mortality. Objectives:The objective of the study was to evaluate biomarkers, such as cardiac troponin T (cTnT) and N-terminal pro-B-type natriuretic peptide (NT-proBNP), to identify RTRs who are at greater risk of experiencing unfavorable outcomes, including all-cause mortality, renal, and cardiovascular (CV) events, as well as to assess the influence of these biomarkers on overall and death-censored graft survival. Patients and methods: A total of 342 stable RTRs were enrolled in this study, with a mean follow-up time of 48.4 months and a median follow-up time of 54 months. The probability of death, CV, and renal events at 4.9 years was calculated using Kaplan-Meier analysis for the group defined by cTnT and NT-proBNP levels above the cutoff values. Results: The probability of death and CV events was 23%, 20%, and 29%, 19%, for cTnT and NT-proBNP levels above the cutoff values, respectively. The probability of renal events, overall graft survival, and death-censored graft survival was 68%, 38%, 25%, and 63%, 41%, 27%, respectively. Conclusion: These data suggest that cTnT and NT-proBNP could potentially identify patients at high risk for death, CV, and renal events, as well as for overall graft survival and death-censored graft survival.

Abstract: Historically, colorectal liver metastases (CRLM) have been considered a contraindication for liver transplantation (LT), given the limited organ supply and poor oncologic efficacy. Recent studies, however, have demonstrated that highly selected patients with unresectable CRLM can achieve remarkable long-term survival following LT, often surpassing outcomes seen with conventional systemic therapies. This review explores the evolving role of LT for the treatment of unresectable CRLM, examining patient selection criteria, overall survival, disease-free survival, recurrence patterns, and emerging biomarkers that may guide transplant eligibility. Additionally, we discuss innovations in organ utilization, including living donor LT and machine perfusion strategies, to expand access while mitigating ethical concerns surrounding organ allocation. As LT for CRLM transitions from experimental protocols to clinical application, this review highlights key challenges and opportunities that will shape its future role in the field of transplant oncology.

Abstract: A 51-year-old gentleman with complications from long-standing type 1 diabetes mellitus underwent simultaneous pancreas and pre-emptive kidney transplant at our center, with rabbit anti-thymocyte globulin and methylprednisolone induction. Three months post-transplant he presented for evaluation of a diffuse lichenoid cutaneous eruption. An extensive infectious workup was unrevealing, and skin punch biopsy showed interface vacuolar dermatitis, suggestive of graft-versus-host disease (GVHD) of the skin. The patient then developed acute transaminitis and neutropenia, and biopsies of the liver and bone marrow done subsequently also confirmed GVHD of these organs, with microchimerism assay on the bone marrow showing 43% of the T-cells were of donor origin. The patient improved with initiation of systemic and topical corticosteroids. Although a rare entity in solid organ transplantation, this case highlights the importance of considering solid organ transplant graft-versus-host disease (SOT-GVHD), particularly in patients receiving organs with a larger volume of passenger donor T-lymphocytes, such as is seen with intestinal, liver, and pancreas transplants.

Abstract: Background/Objectives: Hip fractures in elderly patients are associated with high morbidity and mortality, often worsened by perioperative anemia. Although restric-tive transfusion strategies are recommended, the role of preoperative intravenous iron, particularly ferric carboxymaltose (FCM) remains unclear. This study aimed to investigate whether preoperative IV FCM reduces mortality and transfusion requirements in geriatric hip fracture patients managed under a restrictive transfusion strategy. Methods: In this prospective, randomized controlled trial, 220 patients aged ≥65 years undergoing surgery for hip fractures were allocated to receive either a single 1000 mg dose of intravenous FCM approximately 12 hours before surgery or no iron supplementation. All patients were managed with a standardized restrictive transfusion strategy. Primary outcome was all-cause mortality at 6 and 12 months. Secondary outcomes included perioperative transfusion requirement, hemoglobin trends, and length of hospital stay. Results: The FCM group demonstrated significantly lower mortality at both 6 months (22.9% vs. 39.0%, p = 0.011) and 12 months (28.4% vs. 42.9%, p = 0.028) compared to the control group. Multivariate logistic regression identified preoperative FCM administration as an independent protective factor for mortality. Transfusion rates (30.9% vs. 45.5%, p = 0.02) and transfusion index were significantly lower in the FCM group. Discharge hemoglobin levels were higher in the FCM group, although no significant difference was observed at 6-week follow-up. The difference in hospital stay duration did not reach statistical significance. Conclusions: Preoperative intravenous FCM administration could reduce both short- and long-term mortality and transfusion needs in geriatric hip fracture patients managed under a restrictive transfusion protocol. These findings support further investigation of FCM as a component of perioperative blood management in this high-risk population.

Abstract: Allogeneic hematopoietic stem cell transplantation (HSCT) offers curative potential for various hematologic malignant and non-malignant disorders. Improvements in supportive care, conditioning regimens and graft versus host disease (GVHD) prophylaxis has resulted in a reduction in non-relapse mortality and improved overall survival (OS). Despite this the early-post transplant period is fraught with complications that can significantly impact morbidity and mortality. This review adopts a symptom-based approach using clinical vignettes to highlight five critical early complications of allogeneic HSCT: neutropenic fever, GvHD, posterior reversible encephalopathy syndrome (PRES), transplant associated thrombotic microangiopathy (TA-TMA) and sinusoidal obstructive syndrome (SOS). Each of these conditions presents with non-specific symptoms – such as fever, hypertension, mental status changes or jaundice- requiring high clinical suspicion and timely intervention. Using clinical vignettes, we explore the pathophysiology, clinical presentation, diagnostic challenges and evidence-based management strategies for these complications. The goal is to equip clinicians with a practical framework for recognizing and managing these high-risk syndromes occurring in the early post-transplantation period.

Abstract: Ischemia reperfusion injury (IRI) is the primary cause of early complications in kidney transplantation and contributes to impaired graft function. Mitochondrial DNA (mtDNA), which is implicated in IRI-induced organ damage, has shown promise as a biomarker for assessing and predicting organ function, though studies have focused primarily on deceased donor transplantation. This post-hoc analysis of the VAPOR-1 study aimed to explore the presence, dynamics, and prognostic value of urinary mtDNA in a cohort of 57 living donor kidney transplantation (LDKT) recipients. Surprisingly, higher mtDNA gene levels in the first urine after transplantation were associated with higher estimated glomerular filtration rate (eGFR) at 12 as well as with an increase in eGFR between month 1 and month 24 after transplantation. These findings suggest that, contrary to prior assumptions of mtDNA as a damage marker, early urinary mtDNA levels may reflect graft function rather than injury. This study underscores the potential of urinary mtDNA as a prognostic tool for renal allograft function and advocates for a re-evaluation of the way early posttransplantation renal damage markers are interpreted in LDKT.

Abstract: Ojectives: This article explores the use of ECMO therapy in patients with end-stage respiratory failure who need lung transplantation (LTx). ECMO has several roles: it can bridge patients to transplantation, condition the lung graft in case of primary graft dysfunction after transplantation, condition the the left ventricle after transplantation in patients with primary pulmonary hypertension, and manage COVID-19 patients who are awating LTx or undergoing LTx. Methods: We present 6-year results from high volume lung transplant center (219 cases/6years, >50 cases/2022). We used ECMO in 56 cases (25.6%) of all lung transplants be- tween 2018 and 2023. Results: The one-year survival rate of patients transplanted on ECMO was 85.7% (95%CI: 77.0-95.4%). We applied all advanced ECMO techniques, such as bridging to transplantation on ECMO (n=15, early survival 66.7%), left ventricular conditioning after LTX with VA-ECMO protocol (n=12, 60-day and one-year survival 85.1% and 53% respectively) and the most advanced variant of ECMO such as Sport-ECMO (n=7). We also bridged to transplantation patients with COVID-19 and were among the first in Europe to transplant them from ECMO (n=9, early survival 55%). Conclusions: This article shows possible applications of ECMO therapy for various indications in lung transplant patients and along with data from other publications it demonstrates that ECMO can improve survival and outcomes in patients with end-stage respiratory failure, primary pulmonary hypertension, and COVID-19. The COVID-19 pan- demic highlighted new utilization of ECMO,demonstrating its usefulness and importance in critical care medicine. Further research into the capabilities of the ECMO system may expand knowledge about its role in lung transplantation and future treatments.

Abstract: Background/Objectives: Liver transplantation (LT) is often complicated by severe bleeding and coagulopathy. Viscoelastic testing (VET) offers real-time, bedside assessment of coagulation and may improve transfusion management compared to standard tests. This study evaluates the clinical impact of VET implementation during liver transplantation on bleeding, transfusion requirements, complications, and mortality. Methods: We conducted a single-center before-and-after study comparing patients undergoing LT before and after the implementation of VET. All procedures were performed by the same surgical and anesthetic team using a standardized protocol. Data were collected retrospectively for the Before VET group and prospectively for the After VET group. We compared transfusion requirements, bleeding, complications, and mortality. Results: A total of 59 patients were included, 22 in the After VET group and 37 in the Before VET group. VET implementation was associated with lower intraoperative blood loss (median 4000 mL vs. 6000 mL, p = 0.017) and reduced allogeneic blood transfusion (670 mL vs. 1000 mL, p = 0.008). FFP (0.23 vs. 1.59 units, p = 0.007) and platelet use (0.68 vs. 1.81 units, p = 0.035) were also significantly lower in the VET group, while fibrinogen use was higher (3.00 g vs. 2.00 g, p = 0.036). No differences were observed in complication rates or mortality at 30 days and 1 year. Conclusions: VET significantly improved transfusion precision and individualized coagulation management during LT, leading to reduced use of blood products. These findings support the adoption of VET as a standard of care in LT protocols, as it may enhance patient safety, even though no differences in postoperative complications or mortality were observed.

Abstract: Kidney transplantation significantly improves outcomes in patients with end-stage renal disease; however, postoperative complications remain a substantial concern. This review summarizes the incidence, risk factors, and management strategies for common complications after kidney transplantation. Reported incidence varies widely due to differences in definitions, diagnostic methods, and study designs. Ureteral stenosis occurs in 2.8–18.0% of recipients, vesicoureteral reflux in 0.5–86%, and urinary leakage in 1.1–7.2%. Lymphatic complications, including lymphocele and lymphorrhea, range from 0.6% to 35.2%, with one-third requiring intervention. The incidence of urinary tract infections ranges from 20–43%, while asymptomatic bacteriuria is reported in up to 53% of recipients. Surgical site infections have a median incidence of 3.7%, and incisional hernias develop in 2.5–10% of cases, depending on follow-up duration. Vascular complications affect approximately 10% of recipients, with renal artery stenosis and thrombosis being the most prevalent. Neurologic complications, such as femoral nerve palsy and immunosuppression-related neurotoxicity, though less frequent, can impair recovery. Management strategies vary depending on severity, ranging from observation to surgical intervention. Preventive measures—including optimized ureteral stenting protocols, early catheter removal, careful immunosuppression, and appropriate antimicrobial use—play a crucial role in reducing complication risk. Despite advances in transplantation techniques and perioperative care, these complications continue to affect graft survival and patient outcomes. Further research is needed to standardize definitions and establish evidence-based protocols.

Abstract:

In the application of the digital twin model of the digging face, the data transmission will be transmitted to the remote control platform through the physical hardware via the gateway, and this cross-system and cross-software data transmission mode will inevitably generate the transmission delay, which leads to a certain spatial-temporal deviation between the virtual scene of the remote control platform and the physical digging site. In this paper, by analyzing the operation process of roadheading equipment, a state evolution dynamics model construction method for roadheading equipment is proposed, which includes three stages, namely, discretization of positional state based on cutting path planning, event-driven construction of cutting state evolution map of roadheading equipment and real-time data-driven dynamics evolution of roadheading equipment, and the construction of roadheading equipment state evolution dynamics model provides the best solution for the roadheading equipment. The construction of the model provides theoretical basis and technical support for the construction and alignment of the digital twin multidimensional model of the roadheading equipment.