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Preprint ARTICLE | doi:10.20944/preprints202404.0994.v1

Tea Friščić, Edvard Galić, Domagoj Vidović, Petrana Brečić, Igor Alfirević

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: plasminogen activator inhibitor-1; obstructive sleep apnea; continuous positive airway pressure; atherogenesis; fibrinolytic capacity

Online: 16 April 2024 (07:41:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202312.1993.v1

Roles of Glutathione and AP-1 in the Enhancement of Vitamin D-induced Differentiation by Activators of the Nrf2 Signaling Pathway in Acute Myeloid Leukemia Cells

Yasmeen Jramne-Saleem, Michael Danilenko

Subject: Medicine And Pharmacology, Oncology And Oncogenics Keywords: acute myeloid leukemia; buthionine sulfoximine; carnosic acid; monomethyl fumarate; vitamin D receptor; activator protein-1.

Online: 26 December 2023 (12:15:44 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.0148.v1

Microstructure Evaluation of Fly Ash Geopolymers Alkali-Activated by Binary Composite Activators

Jiangping Zhao, Yachao Wang

Subject: Chemistry And Materials Science, Materials Science And Technology Keywords: Alkali-activated; Microstructure; Fly ash; Binary activator; Crosslinking

Online: 2 June 2023 (07:28:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201709.0143.v1

A Marine Fibrinolytic Compound FGFC1 Stimulating Enzymatic Kinetic Parameters of a Reciprocal Activation System Based on a Single Chain Urokinase-Type Plasminogen Activator and Plasminogen

Guo Ruihua, Duan Dong, Shaotong Hong, Yu Zhou, Fang Wang , Shujun Wang, Wenhui Wu, Bin Bao

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: fibrinolytic acticity; FGFC1; plasminogen activator; plasminogen; enzymatic kinetic

Online: 28 September 2017 (09:17:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202205.0280.v1

Analysis of the Structural Protein Effects Caused by the PURA p.Phe233del Mutation Associated to Cognitive Developmental Delay Using Artificial Intelligence and Hybrid Quantum Mechanics-Molecular Mechanics Modelling

Juan Javier Lopez-Rivera, Luna Rodríguez-Salazar, Alejandro Soto-Ospina, Carlos Estrada-Serrato, David Serrano, Henry Mauricio Chaparro-Solano, Olga Londoño, Paula A. Rueda, Geraldine Ardila, Andrés Villegas-Lanau, Marcela Godoy-Corredor, Mauricio Cuartas, Jorge I. Vélez, Oscar M. Vidal, Mario A. Isaza-Ruget, Mauricio Arcos-Burgos

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: purine-rich element binding protein A gene; PURA; MIM: 600473; transcriptional activator protein Pur-alpha; cognitive developmental delay; mental retardation; Mutation c.697_699del p.Phe233del

Online: 20 May 2022 (16:58:04 CEST)

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A whole-exome capture and next-generation sequencing applied to an 11 y/o patient with a clinical history of congenital hypotonia, generalized motor and cognitive neurodevelopmental delay, severe cognitive deficit, without any identifiable Syndromic pattern, and to her parents, disclosed a de novo heterozygous pathogenic mutation, c.697_699del p.Phe233del (rs786204835)(ACMG classification PS2, PM1, PM2, PP5), harbored in the PURA gene (MIM*600473) (5q31.3), associated to Autosomal Dominant Mental Retardation 31 (MIM # 616158). We used the significant improvement in the accuracy of protein structure prediction recently implemented in AlphaFold that incorporates novel neural network architectures and training procedures based on the evolutionary, physical, and geometric constraints of protein structures. The wild-type (WT) sequence and the mutated one, missing the Phe233, were reconstructed. The predicted local Distance Difference Test (lDDT) for the PURA WT and the PURA-Phe233del showed that the occurrence of the Phe233del affects between 220-320 amino acids. The distortion in the PURA structural confor-mation in the ~5Å surrounding area after the p.Phe233del produces a conspicuous disruption of the repeat III, where the DNA and RNA helix unwinding capability occurs. PURA Protein-DNA Docking corroborated these results in silico Analysis that showed a loss of the contact of the PURA-Phe233del III repeat domain model with the DNA. Together, i) the energetic and stereochemical, ii) the hydropathic indices and polarity surfaces, and iii) the hybrid Quantum Mechanics-Molecular Mechanics (QM-MM) analyses of the PURA molecular models demarcate at the atomic resolution the specific surrounding region affected by these mutations and paves the way for future cell-based functional analysis. To the best of our knowledge, this is the first report of a de novo mutation underpinning a PURA syndrome in a Latin American patient and highlights the importance of predicting the molecular effects in protein structure using artificial intelligence algorithms and molecular and atomic resolution stereochemical analyses.

Preprint REVIEW | doi:10.20944/preprints202310.1953.v1

Hydrogen: A Rising Star in Gas Medicine as a Mitochondria-Targeting Nutrient via Activating Keap1-Nrf2 Antioxidant System

Danyu Cheng, Jiangang Long, Lin Zhao, Jiankang Liu

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Hydrogen; gas medicine; antioxidant; mitochondria; Keap1-Nrf2; Nrf2 activator

Online: 31 October 2023 (03:00:32 CET)

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Preprint ARTICLE | doi:10.20944/preprints202107.0611.v1

Doxycycline and Minocycline act as Positive Allosteric Modulators of the PAC1 Receptor and Induce Plasminogen Activators in RT4 Schwann Cells

Sarah Thomas Broome, Giuseppe Musumeci, Alessandro Castorina

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: pituitary adenylate cyclase-activating peptide (PACAP); PAC1 receptor; doxycycline; minocycline; positive allosteric modulator; Schwann cells; tissue plasminogen activator (tPA); urokinase plasminogen activator (uPA)

Online: 27 July 2021 (21:51:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202110.0293.v1

Safety and Efficacy of Adjunctive Catheter-Directed Thrombolysis During Primary PCI for ST-segment Elevation Myocardial Infarction With High Thrombus Burden

Satsuki Noma, Hideki Miyachi, Isamu Fukuizumi, Junya Matsuda, Hideto Sangen, Yoshiaki Kubota, Yoichi Imori, Yoshiyuki Saiki, Yusuke Hosokawa, Shuhei Tara, Yukichi Tokita, Koichi Akutsu, Wataru Shimizu, Takeshi Yamamoto, Hitoshi Takano

Subject: Medicine And Pharmacology, Cardiac And Cardiovascular Systems Keywords: high coronary thrombus burden; tissue plasminogen activator; catheter-directed thrombolysis

Online: 20 October 2021 (12:53:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201906.0048.v1

Clinical Risk Factors that Predict Functional Recovery in Ischemic Stroke Patients with Pre-Stroke Depression

Leah Wormack, Brice Blum, Benjamin Bailes, Thomas Nathaniel

Subject: Medicine And Pharmacology, Clinical Medicine Keywords: Ambulation, Ischemic stroke, pre-stroke depression, recombinant tissue plasminogen activator (rtPA)

Online: 6 June 2019 (10:37:13 CEST)

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Preprint REVIEW | doi:10.20944/preprints202312.0917.v1

New-Generation Glucokinase Activators: Potential Game-Changers in Type 2 Diabetes Treatment

Dania Haddad, Vanessa Sybil Dsouza, Fahd Al-Mulla, Ashraf Al Madhoun

Subject: Medicine And Pharmacology, Medicine And Pharmacology Keywords: Dorzagliatin; TTP399; glucokinase activator; Type 2 Diabetes; clinical trials; safety; efficacy

Online: 13 December 2023 (04:55:36 CET)

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Preprint ARTICLE | doi:10.20944/preprints202304.0871.v1

Using Biostimulants and Soil Additives to Improve Corn Yield in South Texas

W. James Grichar, Travis W. Janak, Joshua A. Mcginty, Michael J. Brewer

Subject: Environmental And Earth Sciences, Soil Science Keywords: Fungicide; inoculant; insecticide; microbial enhancer; soil activator; soil conditioner; soil stimulant

Online: 25 April 2023 (03:26:11 CEST)

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Preprint REVIEW | doi:10.20944/preprints202002.0458.v1

Tuning up Transcription Factors for Therapy

Attila Becskei

Subject: Biology And Life Sciences, Biophysics Keywords: LacI; Lac repressor; TetR; TAL-Effector; transcription activator-like effector; Cas9d; dissociation rate constant

Online: 29 February 2020 (09:13:12 CET)

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Preprint REVIEW | doi:10.20944/preprints202311.1691.v1

Synephrine and Its Derivative CpdA: Common and Specific Biological Effects

Svetlana A. Dodonova, Ekaterina Zhidkova, Alexey A. Kryukov, Timur Valiev, Kirill I. Kirsanov, Evgeny P. Kulikov, Irina V Budunova, Marianna G. Yakubovskaya, Ekaterina A. Lesovaya

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: synephrine; glucocorticoids; selective glucocorticoid receptor activator (SEGRA); inflammation; cancer; CpdA; metabolic disorders; cardiovascular system; beta-adrenergic receptors

Online: 27 November 2023 (09:16:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202004.0165.v2

The Covid-19 Virus Double Pathogenic Mechanism. A New Perspective

Carlo Brogna

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: Covid-19; Blastn; toxins protein; ACh; aChE; BuChE; CBP; Zero Point; bungaro-toxins, phospholipase A2; similar prothrombin activator protein

Online: 19 April 2020 (06:51:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1368.v2

Activation of the Immune System against the Background of Its Imbalance Caused by Agents of Radiation Nature

Konstantin Vagin, Timur Gaynutdinov, Gulnaz Rakhmatullina, Rustam Nizamov, Marina Gallyamova, Konstantin Sychev, Vladimir Semenov

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: activator of the immune system; subpopulations of lymphocytes; polyglobulins; T-helpers (CD4); T-suppressors (CD8); radioprotector; hemoprotector; immunohemopoiesis

Online: 1 November 2023 (05:25:53 CET)

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Preprint ARTICLE | doi:10.20944/preprints202306.1455.v1

Nebulized recombinant tissue plasminogen activator (rt-PA) for acute COVID-19-induced severe respiratory failure: an exploratory proof of concept trial

Pratima Chowdary, Banwari Agarwal, Maria Rita Peralta, Sanjay Bhagani, Simon Lee, James Goldring, Marc Lipman, Emal Waqif, Mark Phillips, Helen Philippou, Jonathan H Foley, Nicola J Mutch, Robert Ariens, Kathleen A Stringer, Federico Ricciardi, Marie Watissée, Derralynn Hughes, Amit Nathwani, Anne Riddell, David Patch, Jim Buckley, Mark De Neef, Rahul Dimber, Cecilia Diaz-Garcia, Honey Patel, Aarti Nandani, Upuli Dissanayake, Nick Chadwick, Ahmed Alkhatip, Peter Watkinson, Eamon Raith, Suveer Singh, Tony Wolff, Rajeev Jha, Simon E Brill, Ameet Bakhai, Alison Evans, Farhat Gilani, Keith Gomez

Subject: Medicine And Pharmacology, Pulmonary And Respiratory Medicine Keywords: Acute respiratory illness; critical care; recombinant tissue plasminogen activator; nebulization, fibrinolytics, COVID-19 pandemic, inhaled medication, targeted therapy

Online: 20 June 2023 (14:59:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202308.0382.v1

New Immunohistochemical Markers for Pleural Mesothelioma Subtyping

Iosè Di Stefano, Greta Alì, Anello Marcello Poma, Rossella Bruno, Agnese Proietti, Cristina Niccoli, Carmelina Cristina Zirafa, Franca Melfi, Maria Giovanna Mastromarino, Marco Lucchi, Gabriella Fontanini

Subject: Medicine And Pharmacology, Pathology And Pathobiology Keywords: pleural mesothelioma; subtypes; immunohistochemistry; Mesothelin; Claudin-15; Complement Factor B (CFB); Plasminogen Activator Inhibitor 1 (PAI1); p21-activated kinase 4 (PAK4).

Online: 4 August 2023 (12:34:31 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202310.1322.v1

Drynaria fortunei-Derived Polysaccharides Attenuated RANKL-Induced Osteoclast Differentiation by Modulating NFATc1

Jin Ah Ryuk, Ami Lee, Taesoo Kim, Youn-Hwan Hwang, Hyunil Ha

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Drynaria fortunei-derived polysaccharides; bone marrow-derived macrophages; osteoclastogenesis; receptor activator of nuclear factor-κB ligand; nuclear factor of activated T cells 1

Online: 20 October 2023 (12:08:32 CEST)

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Preprint ESSAY | doi:10.20944/preprints202312.1863.v1

Renaming ‘Chemosensory’ Proteins (CSPs): Lipid/Nucleotide-Binding Proteins — Molecular Nomenclature, Structure, Expression, Function, Evolutionary Networks, Clinical Diseases and Associated Molecular Medicine

Guoxia Liu, Botong Sun, Wei Fan, Shousong Yue, Qiuxia He, Jean-François Picimbon

Subject: Biology And Life Sciences, Insect Science Keywords: 4 cysteines soluble proteins; transcription initiation factor; actin skeleton regulatory complex; nuclear pore complex protein; rho gtpase activator; mucin; lipid metabolism; immune response

Online: 27 December 2023 (05:39:13 CET)

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Preprint ARTICLE | doi:10.20944/preprints202002.0003.v1

Antiparkinson Drug Benztropine Suppresses Tumor Growth, Circulating Tumor Cells, and Metastasis by Acting on SLC6A3 and Reducing STAT3

Chiharu Sogawa, Manh Tien Tran, Masayuki Ishige, Kilian Trin, Yuka Okusha, Eman Ahmed Taha, Yanyin Lu, Hotaka Kawai, Norio Sogawa, Masaharu Takigawa, Stuart K. Calderwood, Kuniaki Okamoto, Ken-ichi Kozaki, Takanori Eguchi

Subject: Medicine And Pharmacology, Pharmacology And Toxicology Keywords: drug repositioning/repurposing; dopamine transporter (DAT); benztropine; tumoroids; signal transducer and activator of transcription (STAT); circulating tumor cells (CTC); three-dimensional (3D) culture

Online: 3 February 2020 (03:16:54 CET)

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Preprint ARTICLE | doi:10.20944/preprints201808.0463.v1

Molecular Docking, Computational and Antithrombotic Studies of Novel 1,3,4-oxadiazole Derivatives

Majda Batool, Affifa Tajammal, Firdous Farhat, Francis Verpoort, Zafar A. K. Khattak, Mehr-un- Nisa, Muhammad Shahid, Hafiz Adnan Ahmed, Munawar Ali Munawar, Muhammad Zia ur. Rahman, Muhammad Asim Raza Basra

Subject: Chemistry And Materials Science, Medicinal Chemistry Keywords: Factor Xa ( F-Xa), Cardiovascular diseases (CD), Coronary heart disease (CHD), Tissue plasminogen activator (t-PA), Urokinase (UK), Streptokinase (SK), N,N-Dimethyl formamide (DMF)

Online: 27 August 2018 (14:54:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints201802.0165.v1

The Importance and Effectiveness of Carotid Endarterectomy in Ischaemic Stroke or TIA with Significant Carotid Stenosis

Muhammad Shohel Almamun, Mobashshereen Khan

Subject: Medicine And Pharmacology, Neuroscience And Neurology Keywords: Cerebral autosomal dominant arteriopathy with subcortical infarcts and leuco encephalopathy (CADASIL); Carotid Endarterectomy (CEA); Modified Rankin Scale (MRS); Computed Tomography(CT); Tissue plasminogen activator (tPA); Diffusion weighted Imaging (DWI); Recognition of Stroke in the Emergency Room (ROSIER) scale; Magnetic resonance Imaging (MRI); Internal Carotid Artery (ICA)

Online: 26 February 2018 (11:46:47 CET)

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