Ryuk, J.A.; Lee, A.; Kim, T.; Hwang, Y.-H.; Ha, H. Polysaccharides Derived from Drynaria fortunei Attenuated Osteoclast Differentiation Induced by Receptor Activator of Nuclear Factor-κB Ligand by Modulating NFATc1 and c-Fos. Appl. Sci.2023, 13, 12201.
Ryuk, J.A.; Lee, A.; Kim, T.; Hwang, Y.-H.; Ha, H. Polysaccharides Derived from Drynaria fortunei Attenuated Osteoclast Differentiation Induced by Receptor Activator of Nuclear Factor-κB Ligand by Modulating NFATc1 and c-Fos. Appl. Sci. 2023, 13, 12201.
Ryuk, J.A.; Lee, A.; Kim, T.; Hwang, Y.-H.; Ha, H. Polysaccharides Derived from Drynaria fortunei Attenuated Osteoclast Differentiation Induced by Receptor Activator of Nuclear Factor-κB Ligand by Modulating NFATc1 and c-Fos. Appl. Sci.2023, 13, 12201.
Ryuk, J.A.; Lee, A.; Kim, T.; Hwang, Y.-H.; Ha, H. Polysaccharides Derived from Drynaria fortunei Attenuated Osteoclast Differentiation Induced by Receptor Activator of Nuclear Factor-κB Ligand by Modulating NFATc1 and c-Fos. Appl. Sci. 2023, 13, 12201.
Abstract
The rhizome of Drynaria fortunei (Kunze ex Mett.) J. Sm., which is known as “Golsebo” in Korea, has been traditionally used to treat various inflammatory conditions, including bone metabolism disorders. It relieves blood extravasation, stops bleeding, repairs broken bone tissue, treats bone fractures, and kills bacteria. Recently, D. fortunei-derived polysaccharides (DFP) have been identified as bioactive compounds that act against numerous human diseases. However, the molecular mechanism underlying the bone metabolism-improving effect of DFP has not been elucidated. In this study, we evaluated the modulatory effects of DFP on the differentiation of bone marrow-derived macrophages into osteoclasts. We performed tartrate-resistant acid phosphatase assays using DFP (different concentrations and molecular weights) to evaluate the degree of bone resorption in the receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis of bone marrow-derived macrophages. DFP significantly inhibited RANKL-induced osteoclastogenesis and suppressed RANKL-mediated overexpression of c-Fos and nuclear factor of activated T cells 1, thereby downregulating osteoclast-specific gene (Atp6v0d2, DC-STAMP, and cathepsin K) expression. DFP has potential as a nutraceutical candidate for treating bone loss diseases, including osteoporosis in postmenopausal women.
Keywords
Drynaria fortunei-derived polysaccharides; bone marrow-derived macrophages; osteoclastogenesis; receptor activator of nuclear factor-κB ligand; nuclear factor of activated T cells 1
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
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