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Review
Computer Science and Mathematics
Artificial Intelligence and Machine Learning

Maryam Assafo

,

Peter Langendoerfer

Abstract: Tool condition monitoring (TCM) is essential for ensuring good quality products, machining reliability, efficiency, and sustainability. Machine learning (ML), including deep learning (DL), has been extensively used in the literature to address different TCM tasks such as tool state recognition, tool wear prediction, and remaining useful life prediction. Nevertheless, the adoption of existing methods in real-world manufacturing is still hurdled by different practical challenges. This paper focuses on two key challenges facing ML-based TCM: 1) Variability of operating conditions; 2) Data scarcity. The first challenge arises from the variations in data distributions (domain shift) caused under cross operating conditions, which leads a trained ML model to generalize poorly on data from operating conditions unseen during training. The second challenge stems from the impracticality of collecting sufficient data on real-world shop floors, especially when labeled data is needed. Addressing simultaneously both challenges inherently leads to problem and evaluation settings different from those concerning only one single challenge without having the constraints imposed by the other (e.g., addressing the domain shift assuming the availability of sufficient data, or addressing the data scarcity under single-operating conditions). This paper presents a review focused on TCM considering both different operating conditions and data scarcity scenarios. The works reviewed are based on adaptation- and/or generalization oriented solutions leveraging prior knowledge across various related-yet-distinct learning settings, namely transfer learning, domain adaptation, domain generalization, meta-learning, and hybrid settings. Future research opportunities are also presented. This review can serve as a guide for both researchers and practitioners, presenting state-of-the-art practices and concrete insights to tackle and advance the challenging industry application of TCM.

Article
Environmental and Earth Sciences
Other

Janyne Soares Braga Pires

,

Francine Bonomo Crispim Silva

,

Maria Eduarda da Silva Barbosa

,

Geovana Ribeiro Cavilha

,

Mateus Moura Coelho

,

Samile Mardegan Otilia

,

Fernando Gomes Hoste

,

Ana Júlia Câmara Jeveaux-Machado

,

Lúcio de Oliveira Arantes

,

Vinicius de Souza Oliveira

+2 authors

Abstract: Water deficit is one of the main limiting factors for crop establishment and productivity, particularly affecting seed germination and early seedling growth. This study aimed to evaluate the biostimulant effect of Ascophyllum nodosum extract on maize (Zea mays L.) seeds subjected to osmotic stress induced by PEG-6000.Three independent bioassays were conducted under controlled conditions. First, osmotic potentials ranging from 0 to −0.8 MPa were tested to determine stress levels. In the second assay, seeds were treated with increasing doses (0 to 2 mL kg⁻¹) of a commercial seaweed extract and its isolated mineral fraction. In the third assay, selected doses were evaluated under no stress, moderate stress, and severe stress conditions. Germination percentage, normal and abnormal seedlings, radicle and epicotyl length, and vigor index were assessed. Osmotic stress significantly reduced germination and seedling growth, particularly at −0.6 and −0.8 MPa. Seed treatment with A. nodosum did not affect final germination but improved seedling growth and vigor, showing a dose-dependent response. Maximum efficiency was observed at intermediate doses (~0.45–0.66 mL kg⁻¹), which increased the percentage of normal seedlings and promoted root and shoot development. Under water stress conditions, the complete extract outperformed the mineral fraction, indicating that the beneficial effects are mainly associated with bioactive organic compounds. These findings demonstrate that A. nodosum extract is a promising strategy to mitigate water stress effects during maize seed germination, provided that optimal doses are used.

Article
Arts and Humanities
Archaeology

Robert Jan Duchateau

Abstract: Single-coin finds are increasingly valued as a source for understanding patterns of human activity in the Early Middle Ages. This pilot study examines whether the chronological evenness of single coins can serve as a quantitative proxy for persistent human occupation and landscape stability. Six sites in the Netherlands (450–1200 CE) are compared: three located near relatively stable Pleistocene topographic features (Noardeast-Fryslân/Dokkum, Nijmegen, and Maastricht) and three in dynamic coastal or near-coastal Holocene landscapes (Waadhoeke/Franeker, Katwijk, and Veere/Domburg). All single finds were assigned to five standardised 150-year periods. Chronological evenness was measured using three complementary indices: standard deviation of percentage shares, Shannon entropy, and cosine similarity to a uniform distribution. Sites were classified as “balanced” when at least two metrics met predefined thresholds. The results demonstrate a clear distinction between the two groups. Assemblages from stable landscape positions show relatively balanced chronological profiles, while those from dynamic coastal zones exhibit strongly peaked distributions dominated by the 600–750 CE period (χ² = 347.00, df = 4, N = 1,702, p < 0.001, Cramér’s V = 0.452). Greater chronological evenness appears linked to proximity to stable geomorphological settings. These findings suggest that single-coin evenness can function as a useful proxy for long-term landscape persistence when combined with geo-archaeological evidence. Limitations include recovery biases and variable sample sizes. The study advocates the development of standardised, open-access single-coin datasets to facilitate broader comparative research.

Article
Environmental and Earth Sciences
Space and Planetary Science

Pauline Teysseyre

,

Carine Briand

Abstract: A significant fraction of the HF waves is absorbed by the lowest ionospheric layer, the D-region. This region is perturbed by solar flares, which notably cause fast increases in the Sun’s X-ray flux. We present here a new chemistry model, the Lower Ionosphere Region — Absorption and Chemistry Modelling (LIR-ACheM), to study the D-region behaviour. It is based on the Mitra-Rowe [] scheme, and takes into account four distinct sources (EUV, Lyman-α, X-rays and cosmic rays) and seven species (electrons, NO+, O2+, O4+, positive cluster ions, O2− and other negative ions). It thus offers a compromise between accuracy and computing time. The D-region sluggishness and its recovery time after a flare are analysed, highlighting the importance of detachment at low altitudes and soft X-ray fluxes above 80 km.

Hypothesis
Computer Science and Mathematics
Computer Networks and Communications

Robert Campbell

Abstract: Anthropic’s April 2026 release of Claude Mythos Preview, and the subsequent emergence of “Mythos-class” as a descriptor for frontier autonomous offensive cyber capability, has prompted institutional response across financial regulation, but no blockchain-specific analytical or policy framework. This paper develops one. We define Mythos-class as a vendor-neutral capability profile comprising five primitives — autonomous discovery at codebase scale, multi-step exploit chaining, agentic execution with tool use, sub-day weaponization, and generality across target classes — and we engage the contested boundary between maximalist and distributional framings of the capability through analysis of independent evaluations by AISI and AISLE. The central thesis the paper defends is friction inversion: the patch primitives, segmentation, vendor-coordinated disclosure, and credential rotation that constrain Mythos-class capability in conventional IT environments are not reduced on-chain but structurally absent, making blockchain systemic exposure differently positioned in kind, not in degree, from enterprise IT exposure. We instantiate the thesis against Bitcoin and Ethereum/L2 architectures and four bridge case studies (Ronin, Wormhole, Nomad, Poly Network) totaling over $1.74 billion in losses. Vendor-neutral defensive and governance frameworks defined against the capability profile rather than against any specific model release are the correct unit of analysis. Recommendations follow for protocol governance, audit cadence, and regulatory posture.

Article
Public Health and Healthcare
Nursing

Kuralai Utzhanova

,

Dinara Makhanbetkulova

,

Gulshara Aimbetova

,

Aurelija Blazeviciene

,

Nargiza Nassyrova

,

Akmaral Khalelova

,

Aizat Aimakhanova

,

Zhenis Mukhamedkerim

Abstract: Background: Adverse event reporting is a critical component of patient safety systems; however, nurses’ engagement in reporting is influenced not only by reporting procedures but also by broader organizational characteristics of the nursing practice environment. Understanding how these organizational factors shape nurses’ perceptions of reporting systems remains insufficiently explored, particularly in post-Soviet healthcare contexts. Objective: This study aimed to examine how characteristics of the nursing professional practice environment are associated with nurses’ perceptions of the benefits of adverse event reporting in healthcare institutions in Kazakhstan. Methods: A cross-sectional survey was conducted among 468 nurses working in healthcare organizations across six major cities in Kazakhstan. The professional practice environment was assessed using the Revised Professional Practice Environment (RPPE) scale, while attitudes toward adverse event reporting were measured using the Reporting of Clinical Adverse Events Scale (ROCAES), specifically the “perceived benefits of reporting” dimension. Exploratory factor analysis, Spearman correlation analysis, and binary logistic regression were applied. Results: Exploratory factor analysis identified three key dimensions of the professional practice environment: professional motivation and teamwork, interprofessional conflict and communication, and staffing adequacy. Correlation analysis showed that several dimensions of the practice environment were negatively associated with perceived benefits of reporting. However, multivariable regression analysis revealed that cultural sensitivity, internal work motivation, and control over practice were positive predictors of perceived reporting benefits. This contrast between negative bivariate correlations and positive multivariable predictors highlights the complex organizational dynamics underlying nurses’ reporting attitudes. Conclusions: The findings indicate that nurses’ perceptions of adverse event reporting are embedded within a broader organizational ecology of nursing practice. Strengthening supportive professional environments—particularly those promoting motivation, autonomy, and culturally responsive care—may enhance nurses’ engagement in patient safety activities and improve the effectiveness of reporting systems.

Article
Computer Science and Mathematics
Artificial Intelligence and Machine Learning

Krish Mithra Nagamothu

,

Sasank Mahadev

,

M. Tharun Sai

Abstract: This paper presents an adaptive Threat Severity Assessment System (TSAS) designed to support real-time decision-making in defense environments. Existing systems primarily focus on detection and tracking, often lacking a dedicated layer for severity evaluation and prioritization. This limitation can lead to inefficient response strategies in time-critical scenarios.The proposed TSAS framework operates as an intermediate intelligence layer that processes heterogeneous sensor inputs and classifies threats into multiple severity levels using a feed-forward neural network. The system integrates entropy-based concept drift detection and incremental learning to maintain performance under evolving threat conditions. A composite severity score is generated by combining model predictions with proximity-based heuristics.The framework is evaluated using a high-fidelity synthetic dataset, achieving an overall classification accuracy of 96.4% and a millisecond-level end-to-end latency of approximately 4.5 ms under controlled hardware conditions. Experimental results demonstrate improved prioritization capability and robustness compared to traditional rule-based, fuzzy logic, and static machine learning approaches.These findings suggest that TSAS provides a computationally efficient and adaptable solution for real-time threat severity assessment, with potential applicability in defense systems, drone monitoring, and critical infrastructure protection.

Article
Engineering
Electrical and Electronic Engineering

Zhiqiang Gao

,

Bing Ren

,

Jing Han

,

Jie Li

,

Jing Liu

,

Huihui Bai

Abstract: Multimodal sensors can collect multiple signals and have great potential in robotics and other technical fields. However, such sensors often encounter challenges of signal crosstalk and insufficient real-time performance, particularly in the detection of pressure and temperature, which significantly affect measurement accuracy. To address this issue, a multimodal PCSC sensor was developed. This sensor reduces signal crosstalk by separating force and temperature signals. It uses the pressure-resistance variation of carbon quantum dots (CQDs) to detect force and the thermochromic properties of spiropyran (SP) to detect temperature. When pressure and temperature act on the sensor simultaneously, the resistance increases with pressure and stabilizes when the pressure becomes constant. The response time is 0.4 s. As the temperature rises, the resistance decreases, and the color becomes deeper. Both resistance and color stabilize within 7.5 s. To improve temperature sensing accuracy, a lightweight ResNet-Transformer network (LRTNet) was proposed. This algorithm combines ResNet’s ability to extract features and Transformer’s ability to model sequences. It efficiently fuses color and resistance signals for temperature detection. Tests on a robotic manipulator for dual recognition of temperature and force showed that LRTNet achieved a runtime of 152.08 ms and a temperature sensing accuracy of 95%. LRTNet improved overall performance by at least 11% compared to traditional algorithms. The sensor and algorithm improved the performance and reliability of multimodal sensors.

Review
Medicine and Pharmacology
Dermatology

Ryoji Tanei

,

Yasuko Hasegawa

Abstract: The role of IgE‑mediated allergy in atopic dermatitis (AD) has been progressively downplayed as contemporary models emphasize barrier dysfunction, type‑2 cytokine–driven inflammation, pruritus pathways, immune dysregulation, and microbial imbalance. This shift, however, has obscured a defining feature of extrinsic AD: a functional IgE‑dependent amplification loop operating across the epidermal and dermal immune network and extending into the draining lymph node. Emerging evidence shows that Langerhans cells, inflammatory dermal dendritic cells, inflammatory dendritic epidermal cells (IDECs), mast cells, and basophils can acquire environmental allergens through FcεRI‑bound IgE, enabling efficient antigen capture, processing, and T‑cell activation. Among these, IDECs appear central to IgE‑mediated delayed‑type hypersensitivity and the development of spongiosis following epicutaneous allergen exposure. Integrating these findings, we propose a mechanistic model in which IgE‑bearing antigen‑presenting cells initiate and sustain a positive feedback circuit that reinforces type‑2 inflammation and contributes to the chronicity of extrinsic AD. Re‑positioning this IgE‑dependent circuit within the broader pathophysiology of AD provides a revised framework that reconciles classical atopy with modern immunologic insights and highlights new therapeutic opportunities targeting IgE–FcεRI signaling, IDEC biology, and allergen‑driven epidermal immune activation.

Abstract: This study aimed to evaluate the extra-phosphoric effect of increasing doses of bacte-rial phytase (RONOZYME HiPhos) in corn and soybean meal-based diets on perfor-mance, carcass yield, and meat quality in pigs during the nursery, growing, and fin-ishing phases (GT). Two hundred and fifty pigs, castrated males and females, with an initial weight of 6.08 ± 0.748 kg and 21 days of age, were subjected to 5 treatments: PC: positive control diets, supplemented with inorganic phosphorus (P) and calcium (Ca), meeting their full nutritional requirements; NC: negative control diets, with re-duced available phosphorus (-0.18%) and calcium (-0.16%); 1000FYT: NC + 1,000 FYT/kg of feed; 2000FYT: NC + 2,000 FYT/kg of feed; 3000 FYT: NC + 3000 FYT/kg of feed. Average daily gain (ADG) in the nursery phase did not differ between the groups supplemented with 1,000; 2,000 and 3,000 FYT/kg (0.430 kg, 0.441 kg and 0.428 kg respectively) and PC (0.481 kg), but was higher (P< 0.05) than NC (0.398 kg). Feed conversion ratio (FCR) in the same phase was similar between PC (1.546) and the groups supplemented with phytase (1.516; 1.535; 1.519), all being better (P< 0.05) than NC (1.676). The quadratic effect for phytase was verified for FCR in the phase, with the best inclusion of 2,320 FYT/kg of feed. In the GF phases and in the overall experi-mental period (21 to 156 days), the results for daily feed intake (DFI), ADG and FCR favored PC and the groups supplemented with phytase compared to the NC (P< 0.05). A quadratic effect was observed for FCR considering the entire GF phase, with the best inclusion of 1,923 FYT/kg of feed. Groups supplemented with phytase and PC obtained better carcass results compared to NC (P< 0.05). Linear effects were observed to percentage and quantity of lean meat in the carcass. There was no difference be-tween treatments for meat quality. Supplementation with phytase in corn and soy-bean meal-based diets with severely reduced inorganic P and Ca improved pig per-formance at all stages, with optimized inclusion values of approximately 2,200 FYT/kg of feed, and dose-dependent benefits on carcass characteristics.

Review
Biology and Life Sciences
Cell and Developmental Biology

In Young Jo

,

Jin-Woo Kim

,

Beomjong Song

,

Yujeong Song

,

Jae Kyeom Kim

,

Jeong-Oh Shin

Abstract: Taste buds are continuously renewed sensory organs in which development, adult maintenance, and repair share overlapping molecular circuitry. During embryogenesis, WNT/β-catenin signaling promotes taste placode formation and placodal Shh expression, whereas SHH refines papilla spacing and restricts neighboring papilla formation. SOX2 functions as a taste-competence and progenitor-maintenance factor. In adults, LGR5/LGR6-RSPO-WNT signaling sustains progenitor activity, and gustatory neurons provide RSPO2 as a niche signal that maintains epithelial renewal. HH signaling from epithelial and neuronal sources further supports SOX2-dependent progenitor homeostasis. Lineage allocation is controlled by transcriptional programs that include POU2F3/SKN-1a for sweet, umami, and bitter type II taste receptor cells and ASCL1 with posterior-field NKX2-2 for type III presynaptic/sour cells. After denervation or irradiation, regeneration depends primarily on LGR5+/KRT14+ progenitors and may be supplemented, in specific injury contexts, by plasticity of a subset of K8-lineage taste receptor cells that acquire KRT14/SOX2/PCNA progenitor-like features. Key unresolved issues include the direct chromatin targets of taste lineage regulators (which remain to be defined by ChIP-seq in native taste progenitors), the identity of the type I cell selector, the contribution of dedifferentiation across injury models, and the extent to which mouse-derived networks are conserved in human taste biology.

Review
Biology and Life Sciences
Life Sciences

Vikrant Shinde

,

Nilima Harname

,

Pranal Sonawane

,

Gaurav Sangle

,

Jayesh Patil

Abstract: Quantum dots (QDs) are tiny semiconductor particles with unique light and electronic properties that can be adjusted by changing their size. They are widely usedin drug delivery, bioimaging, and theranostic applications. However, designing the best QDs is difficult because there are many possible combinations, makingtraditional trial-and-error methods slow and inefficient. Artificial intelligence (AI) and machine learning (ML) have improved this process by helping scientistspredict properties, design better QDs, and automate experiments. This review explains how various AI methods, including supervised learning, graph neuralnetworks, generative models, Bayesian optimisation, and active learning, are applied to QD-based drug delivery. These approaches have helped improve QDsynthesis, control drug release, and target specific areas such as tumours and the brain. AI has also supported applications in cancer treatment, neurological diseases,infections, and gene delivery. Despite these benefits, there are still challenges, such as a lack of reliable data, difficulty applying models to real-world conditions,and a limited understanding of how AI models make decisions. New technologies such as self-driving labs, advanced AI models, and quantum computing areexpected to further advance this field. Overall, combining AI with nanotechnology is making drug delivery faster, smarter, and more precise.

Article
Physical Sciences
Astronomy and Astrophysics

Mikhail Trofimov

Abstract: This paper presents Timeflow Gravity (TG), a framework in which gravity emerges as a thermodynamic, entropic force driven by the wave mechanics of a continuous $U(1)$ spacetime medium. Building on T. Jacobson’s derivation of Einstein’s equations from the Clausius relation, we derive the Einstein field equations as an emergent macroscopic equation of state. Within this framework, we interpret dark matter and dark energy effects as wave-mechanical projections of the underlying phase space. At galactic scales, constructive phase interference between baryonic matter and the vacuum recovers MOND-like dynamics. By incorporating kinematic phase decoherence, the theory offers a potential resolution to MOND’s mass discrepancy in galaxy clusters, as well as the spatial offset observed in the Bullet Cluster. On cosmological scales, conservation of the one-dimensional topological phase boundary yields parameter-free matter and vacuum density parameters, offering a possible resolution to the cosmological coincidence problem. This result yields a dynamical dark energy equation of state (\( w \approx -0.84 \)) consistent with recent DESI observations. Finally, we establish a falsifiability criterion: the intrinsic scatter of the Radial Acceleration Relation (RAR) should systematically anti-correlate with the local macroscopic kinematic entropy of the system.

Article
Social Sciences
Law

Francesco Alessi Longa

Abstract: This article presents a doctrinal analysis of the way restorative justice has entered the Italian criminal system through Legislative Decree No. 150 of 10 October 2022, the so-called Cartabia reform, as later integrated by Legislative Decree No. 216 of 27 December 2024. The central theme of the paper is the model of complementarity between restorative programs and the ordinary criminal proceeding, considered in the light of Directive 2012/29/EU and Recommendation CM/Rec(2018)8 of the Committee of Ministers of the Council of Europe. After reviewing the notion, the models and the application mechanisms of restorative justice, the article focuses, on the doctrinal plane, on three areas of friction within the new regulatory architecture. They concern the access of restorative programs to all stages of the proceeding, the question of safeguards in cases of intimate partner violence and gender-based crime, and the institutional design of the new Centres for restorative justice. For the third issue, the article keeps its claims at the level of the legislative text and treats any proposition on territorial variation, on the functioning of the Centres or on the implementation deficit as a hypothesis for future empirical research. On the whole, the Italian regulatory intervention looks relevant, albeit with some critical issues, and to be kept under observation for future application developments. In particular, it seems possible to assert that the reform has formally opened the doors to a relational paradigm of justice, but the cultural transition, from a criminal-centric system towards a model of relational justice, will depend, in fact, on the practical choices of judges, mediators and local authorities in the coming years.

Article
Engineering
Mechanical Engineering

Mattia Pelosin

,

Gianluca D’Errico

,

Tommaso Lucchini

,

Paolo Albertelli

Abstract: Heat removal by spray impingement is widely used in different industrial processes. A cooling regime of particular interest occurs when the temperature of the cooled surface exceeds the Leidenfrost temperature of the spray. An accurate numerical model of this cooling regime could help to optimise many industrial applications where spray cooling is used, such as cryogenic machining and spray quenching. In this paper, an Eulerian-Lagrangian Conjugate Heat Transfer (CHT) model designed for spray impingement above the Leidenfrost temperature is proposed. Two different sub-models are implemented to quantify the heat transfer between the droplet and the solid. The heat transfer models are validated through a literature experimental campaign, showing accurate and flexible prediction of heat transfer characteristics across diverse operating conditions, temperature levels, and spray configurations.

Article
Physical Sciences
Theoretical Physics

Jau Tang

Abstract: We present a framework for gravity in which the effective interaction is described by a dynamically generated Yukawa-type potential arising from nonlinear field self-interactions. In this approach, the characteristic scale is not imposed but emerges directly from the field equations, leading to a scale-dependent gravitational interaction. The resulting potential is intrinsically non-perturbative and reduces to standard General Relativity in high-density regimes. We show that this framework naturally reproduces flat galaxy rotation curves and the Tully–Fisher relation, while also providing enhanced gravitational lensing consistent with cluster observations. Using representative fits to dwarf and spiral galaxies, as well as cluster convergence profiles, we demonstrate that a single dynamical mechanism can account for both kinematic and lensing phenomena without invoking dark matter or empirical acceleration scales. These results suggest that gravity may be fundamentally a self-interacting field with an emergent, environment-dependent range.

Article
Computer Science and Mathematics
Artificial Intelligence and Machine Learning

Tanya Khanna

,

Zarak Khan

,

Udit Goel

,

Jim Samuel

,

Julia Esguerra

,

Radha Jaganathan

,

Soumitra Bhuyan

Abstract: Artificial Intelligence (AI) is accelerating societal transformation at an unprecedented pace, generating both utopian aspirations and dystopian anxieties. Human civilization has undergone fundamental changes through every technological revolution starting with the Industrial Age and continuing through the digital era as AI emerges as the next paradigm shift. This paper studies the public discourse on AI by analyzing extensive news headlines on AI using natural language processing (NLP) methods. Our research applies sentiment analysis and topic modeling to a global dataset across education, healthcare, robotics, careers, and society to identify the dominant narratives shaping public perception. Media coverage presents AI as a dual force that brings human benefits and existential dangers according to our research findings. By moving beyond the utopia-dystopia dichotomy, we show that AI's social effects will emerge from the dynamic relationship between governance systems, ethical protections, and human-enhancive AI (HEAI) frameworks. We provide practical insights about AI's future impact and present strategies for maximizing AI benefits while mitigating its risks.

Article
Biology and Life Sciences
Agricultural Science and Agronomy

Sachidanand Nayak

,

Prasad Gandham

,

T. Swaroopa Rani

,

Srinivas Vadlamudi

,

Pradeep Ruperao

,

Rachit Saxena

,

Abhishek Rathore

,

Vivek Thakur

,

Subramanium Gopalakrishnan

Abstract: A rice rhizosphere Streptomyces strain SAI-25 was previously reported to exhibit bicontrol activity against a limited range of agricultural pests, leaving its broader agricultural potential unexplored. In this study, we performed whole genome sequencing, untargeted metabolomics and in-vitro assays to examine its full agricultural potential. Genome similarity confidently re-assigned SAI-25 as a new strain of S. cavourensis. The comparative genome analysis revealed the presence of unique proteins and genomic islands with diverse functions highlighting its genomic novelty. Among the predicted Biosynthetic Gene Clusters (BGCs) for secondary metabolites, majority were annotated having biocontrol and plant growth promoting (PGP) activities. Three of them were detected in untargeted metabolomics of secretome on Iron deficiency or Salinity stress, which includes a siderophore (desferrioxamine B), an osmoprotectant (ectoine), and a broad-spectrum antimicrobial (valinomycin). Beyond the annotated BGCs, at least eight additional agriculturally relevant secondary metabolites were also detected. For the previously reported insecticidal diketopiperazine derivative produced by SAI-25, two key enzymes capable of diketopiperazine core biosynthesis were predicted. Finally, the in-vitro assays revealed its broad range PGP activities. Overall, the SAI-25’s versatile secondary metabolites and potent PGP enzymes highlight its potential as a promising biopesticide/biofertilizer candidate.

Short Note
Chemistry and Materials Science
Organic Chemistry

Nathan Long

,

Emanuela Paval

,

Joseph C. Bear

,

Jeremy K. Cockcroft

,

Stephen P. Wren

Abstract: The title compound 3-(diphenylamino)-4-ethoxycyclobut-3-ene-1,2-dione (6), was prepared by reaction of diphenylamine (2) with diethyl squarate (DES; 5) as part of our ongoing studies on monosquarate-amides. Following purification and recrystallisation, the product was isolated as a green crystalline solid. Its structure was established by spectroscopic methods including: FTIR, 1H NMR, 13C NMR and HRMS and was unambiguously confirmed by single crystal X-ray diffraction. This work provides access to a previously unreported diphenylamino substituted squaric acid derivative.

Article
Chemistry and Materials Science
Physical Chemistry

Fathi Elashhab

,

Lobna Sheha

,

Nada Elzawi

Abstract: Heparin is a highly sulfated polyelectrolyte, and its properties depend a lot on its shape in solution. In this study, we closely examined the structural behaviour of UVC-irradiated low-molecular-weight heparin. By using controlled photodegradation, we created native, small, and ultra-small molar mass fractions, which allowed us to study how structural properties change with molecular weight. We examined how molar mass, radius of gyration, second virial coefficient, and critical overlap concentration are related to one another to understand different conformational states. Our results showed that as molar mass decreased, the chain diameter and persistence length also dropped, while the overlap concentration increased. This means the hydrodynamic volume went down and the chains became more flexible. The positive second virial coefficient values showed that polymer–solvent interactions remained favourable after photo-tailing. The scaling exponents suggest that degraded heparin behaves as a semi-flexible polyelectrolyte and adopts an extended-coil shape in water with electrolytes. Further analysis showed that the characteristic ratio and stiffness of the chains decreased as the chains were broken by irradiation. Overall, UVC phototailing provides a reliable way to modify the structure of these molecules while maintaining solution stability. These findings show a clear link between reduced molecular weight and changes in shape, which is useful for developing better low-molecular-weight heparins for pharmaceutical and medical use.

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