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Preprint HYPOTHESIS | doi:10.20944/preprints202409.1467.v1

Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (Me/Cfs) Hypothesis: Three Subtypes of Noradrenergic Neuron Dysfunction

Tamara Carnac

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Myalgic Encephalomyelitis; Chronic Fatigue Syndrome; ME/CFS; Norepinephrine; Insulin; Neuron

Online: 19 September 2024 (04:31:27 CEST)

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Myalgic Encephalomyelitis (ME), sometimes called Chronic Fatigue Syndrome (CFS), is a complex, debilitating condition characterised by symptoms such as profound fatigue, autonomic dysfunction, gastrointestinal problems, unrefreshing sleep, cognitive problems, exercise intolerance and post-exertional malaise (PEM) (a worsening of symptoms after mild physical or mental effort). The pathophysiology of ME remains poorly understood. This paper proposes a novel hypothesis centred on norepinephrine dysregulation and insulin signaling as the underlying mechanisms contributing to distinct subtypes of ME. I hypothesise that ME can be categorised into three subtypes based on variations in norepinephrine transport and insulin signaling: ME1, ME2, and ME3. In ME1, a higher than normal rate of insulin secretion during the first phase insulin response and/or hypersensitive insulin receptors leads to reduced neuronal norepinephrine reuptake, resulting in elevated extracellular norepinephrine and subsequent down-regulation of beta-2 adrenergic receptors. ME2 is characterised by prolonged insulin release and insulin resistance, which may contribute to the development of postural orthostatic tachycardia syndrome (POTS) and microclot formation. ME3 is marked by reduced norepinephrine synthesis and a resultant deficiency of norepinephrine within neurons. All three subtypes involve a deficiency of norepinephrine inside the noradrenergic neuron which is worsened by physical and mental exertion. There are many similarities between ME and Long COVID. Both have their onset after a virus and have a similar symptom profile, as well as having the same co-occurring conditions such as mast cell activation and POTS. It is quite possible that a subset of Long COVID patients have ME, and so I will be referring to both ME and Long COVID research papers throughout this hypothesis paper. This hypothesis integrates genetic, metabolic, and neuroendocrine data to delineate these subtypes and suggests tailored diagnostic and therapeutic approaches for each. This hypothesis aims to guide future research into the distinct pathophysiological mechanisms of ME and enhance understanding and management of this debilitating condition.

Preprint ARTICLE | doi:10.20944/preprints202409.1463.v1

Hydroxycinnamic Acid Oligomers-Based Biostimulant Nurspray® Enhances Tomato Yield, Fruit Quality, and Reduces Blossom-End Rot

Lien González-Pérez, Eduardo Tejera

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: biostimulant; fruit quality; hydroxycinnamic acid oligomers; tomato

Online: 19 September 2024 (04:29:46 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1461.v1

Effect of Green Cowpea Manure on the Quality Properties of Sandy Soils Under the Specific Conditions of the Dry Climate in Romania

Reta Draghici, Ștefan Nanu, Aurelia Diaconu, Cristina Bîrsoghe, Florentina Netcu, Alina Nicoleta Paraschiv, Milica Dima

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: cowpea; green cowpea manure; crop rotation; fertilization system; soil chemical quality; soil hydrophysical indices

Online: 19 September 2024 (04:28:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1448.v1

Multi-Sensor Soil Probe and Machine Learning Modeling

Sabine Grunwald, MOF Murad, Stephen Farrington, Woody Wallace, Daniel Rooney

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: digital twin, digital soil mapping, soil sensors, multi-sensor system, digital soil core, machine learning, artificial intelligence, soil properties, scale

Online: 18 September 2024 (15:49:20 CEST)

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We present a data-driven, in situ proximal multi-sensor digital soil mapping approach to develop digital twins for multiple agricultural fields. A novel Digital Soil CoreTM (DSC) Probe was engineered that contains seven sensors, each of a distinct modality, including sleeve friction, tip force, dielectric permittivity, electrical resistivity, soil imagery, acoustics, and visible and near-infrared spectroscopy. The DSC System integrates components the DSC Probe, DSC software, and deployment equipment to sense soil characteristics at a high vertical spatial resolution (mm scale) along in situ soil profiles up to a depth of 120 cm in about 60 sec. The DSC Probe in situ proximal data are harmonized into a data cube providing vertical high-density knowledge associated with physical-chemical-biological soil conditions. In contrast, conventional ex situ soil samples derived from soil cores, soil pits, or surface samples analyzed using laboratory and other methods are bound by substantially coarser spatial resolution and multiple compounding errors. Our objective was to investigate the effects of mismatched scale between high-resolution in situ proximal sensor data and coarser resolution ex situ soil laboratory measurements to develop soil prediction models. Our study was conducted in central California soil in almond orchards. We collected DSC sensor data and spatially co-located soil cores that were sliced into narrow layers for laboratory-based soil measurements. Partial Least Squares Regression (PLSR) cross-validation was used to compare results testing four data integration methods. Method A reduced the high-resolution sensor data to discrete values paired with layer-based soil laboratory measurements. Method B used stochastic distributions of sensor data paired with layer-based soil laboratory measurements. Method C allocated the same soil analytical data to each one of the high-resolution multi-sensor data within a soil layer. Method D linked the high-density multi-sensor soil data directly to crop responses (crop performance and behavior metrics) bypassing costly laboratory soil analysis. Overall, the soil models derived from Method C outperformed Methods A and B. Soil predictions derived using Method D were most cost-effective for directly assessing soil-crop relationships, making this method well-suited for industrial-scale precision agriculture applications.

Preprint REVIEW | doi:10.20944/preprints202409.1424.v1

’Seeing is Believing’: How Neutron Crystallography Informs Enzyme Mechanism by Visualizing Unique Water Species

Qun Wan, Brad C. Bennett

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: neutron diffraction; deuterium; hydronium; protein structure; enzyme catalysis; H/D exchange

Online: 18 September 2024 (15:28:49 CEST)

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Hydrogen is the lightest atom and composes roughly half of the atomic content in macromolecules, yet their location can only be inferred or predicted in most macromolecular structures. This is because hydrogen can rarely be directly observed by the most common structure determination techniques (such as X-ray crystallography and electron cryo-microscopy). However, knowledge of hydrogen atom positions, especially for enzymes, can reveal protonation states of titratable active site residues, hydrogen bonding patterns, and the orientation of water molecules. Though we know they are present, this vital layer of information, which can inform a myriad of biological processes, is frustratingly invisible to us. The good news is that, even at modest resolution, neutron crystallography (NC) can reveal this layer and has emerged this century as a powerful tool for elucidating enzyme catalytic mechanisms. Due to its strong and coherent scattering of neutrons, incorporation of deuterium into the protein crystal amplifies the power of NC. This is especially true when solvation and the specific participation of key water molecules are crucial for catalysis. Neutron data allow the modeling of all three atoms in water molecules and have even revealed previously unobserved and unique species such as hydronium (D3O+) and deuteroxide (OD-) ions as well as lone deuterons (D+). Herein, we briefly review why neutrons are ideal probes for identifying catalytically important water molecules and these unique water-like species, limitations in interpretation, and four vignettes of enzyme success stories from disparate research groups. One of these groups was that of Dr. Chris G. Dealwis, who died unexpectedly in 2022. As a memorial appreciation of his scientific career, we will also highlight his interest and contributions to the neutron crystallography field. As both the authors were mentored by Chris, we feel we have a unique perspective on his love of molecular structure and admiration for neutrons as a tool to query those structures.

Preprint REVIEW | doi:10.20944/preprints202409.1422.v1

Accelerating Wound Repair: The Multifaceted Role of Mesenchymal Stem Cells

Abdul Ghani NI, Nurhidayatul MN, Khairul AK, Hasmad HN, Afifa NT, Helmi BS, Aida Juliana AJ, Farisha MN, Balqish MR, Chua KH, BiiChau T, Shamsul BS

Subject: Biology And Life Sciences, Life Sciences Keywords: Mesenchymal Stem Cells (MSCs); Paracrine mechanism; Growth factors; Chronic wounds; Secretome

Online: 18 September 2024 (15:24:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1444.v1

Impaired Development of Collagen Antibody-Induced Arthritis in Rab44-Deficient Mice

Yu Yamaguchi, Tomoko Kadowaki, Eiko Sakai, Mayuko Noguromi, Shun Oyakawa, Takayuki Tsukuba

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Rab44; collagen antibody-induced arthritis; immune cells; osteoclasts

Online: 18 September 2024 (15:11:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1426.v1

Improving the Efficiency and Safety of Sentinel Stink Bug Eggs Using X-Rays

Evelyne Hougardy, Ronald P. Haff, Brian N. Hogg

Subject: Biology And Life Sciences, Insect Science Keywords: stink bug; egg parasitism; field monitoring; sentinel eggs; x-ray irradiation; egg sterilization

Online: 18 September 2024 (13:57:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1416.v1

Cultivating Health: Advancing Medicinal Plant Management Strategies in Iraq

Mahir Essa Jumaa Alsalahi

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Traditional Medicine; Mesopotamian Civilization; Biodiversity; Ethno botany; Environmental Challenges; Herbal Remedies; Plant Compounds; International Trade

Online: 18 September 2024 (13:04:23 CEST)

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Preprint SHORT NOTE | doi:10.20944/preprints202409.1406.v1

Advances in Compounds Targeting Regulatory Mechanisms of Biofilm Formation in Unicellular Eukaryotes: Insights from 2024 Research and Applications in Vietnam

Dang Anh Tuan, Pham Vu Nhat Uyen, Jan Masak

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: biofilm formation; quorum sensing inhibitors; cyclic di-GMP; betulinic acid; pulmonarin B analogues; antimicrobial resistance; unicellular eukaryotes; Vietnam

Online: 18 September 2024 (12:50:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1404.v1

The Impact of the Lighting Regimen and Feeding Program during Rearing on Hy-Line Brown Pullets at the End of Rearing and, During Early Lay

Wendy Isabelle Muir, Yeasmin Akter, Sebastian K.Y. Kho, Kenneth Bruerton, Peter John Groves

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: food restriction; reduced lighting; egg quality; egg production; body weight; feed intake; age of first egg; growth; photoperiod

Online: 18 September 2024 (12:43:15 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202409.1379.v1

A Retrospective Report of Carprofen Administration as Post-Operative Analgesia Reveals Negative Effects of Recommended Doses

Zoë Jäckel, Ahmed Adžemović, Benedikt Kloos, Stefanie Hardung, Rita Sanchez-Brandelik, Philippe Coulon, Ilka Diester

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: analgesia; laboratory animals; animal welfare; multimodal drug administration; stereotactic surgery; refinement

Online: 18 September 2024 (11:59:13 CEST)

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Preprint COMMUNICATION | doi:10.20944/preprints202409.1377.v1

Tellurium-Doped Bioactive Glassinduces Ferroptosis in Osteosarcoma Cells Regardless of FSP1

Elzbieta Pańczyszyn, Mari Lallukka, Mara Gagliardi, Valentina Saverio, Romina Monzani, Marta Miola, Enrica Verné, Marco Corazzari

Subject: Biology And Life Sciences, Life Sciences Keywords: Ferroptosis; Tellurium; osteosarcoma; FSP1; biomaterials

Online: 18 September 2024 (11:55:52 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1351.v1

Functional Morphology of the Mouthparts of Ladybeetle Vibidia duodecimguttata (Coleoptera: Coccinellidae), with Emphasis on Their Sensilla

Long Chen, Yaping Shi, Ke Wang, Yuanxing Sun, Yanan Hao

Subject: Biology And Life Sciences, Insect Science Keywords: mouthparts; sensilla; Vibidia duodecimguttata; scanning electron microscopy

Online: 18 September 2024 (11:37:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1360.v1

Urban Forestry in Sub-Saharan Africa: Challenges, Contributions, and Future Directions for Combating Climate Change and Restoring Forest Landscape

Sawaba ALE, Kossi ADJONOU, Kossi Novigno SEGLA, Kossi KOMI, Jean-Bosco Benewinde ZOUNGRANA, Coffi AHOLOU, Kouami Kokou

Subject: Biology And Life Sciences, Forestry Keywords: Urban forestry; green space; urban heat islands; forest landscape restoration; city planning

Online: 18 September 2024 (11:37:46 CEST)

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Since the 1960s, the countries of sub-Saharan Africa have been affected by an urban crisis due to the demographic explosion in cities and the resulting land pressures. These have resulted in the proliferation of spontaneous housing areas which question the future of urban vegetation and its degree of inclusion in city planning. To analyze the state of knowledge on the research already carried out on the development of urban landscape, particularly in sub-Saharan Africa, a literature review on urban forestry covering the period 1998-2022 was carried out in order to better orient future research works for a greater consideration to urban forestry and their contributions in combating the adverse effects of climate change. Out of a set of 110 scientific papers selected, 50 were considered relevant because of their direct link with urban forestry. From the analysis of this papers, a classification of the articles according to 05 themes was carried out. These are: management of green spaces in urban areas, functions of trees in an urban environment, development of urban forestry, characterization of urban tree flora and financing of urban forestry. Thus, “function of trees in an urban environment and the characterization of urban tree flora” are the most addressed respectively at 42% and 28%. “Financing urban forestry” is practically not addressed (2%). The analysis of the 50 selected articles reveals a significant geographic concentration of urban forestry research. Countries like South Africa, Kenya, and Ghana are more represented in the literature, while vast regions in Central and Western Africa (e.g., Democratic Republic of Congo, Chad, and Niger) have minimal or no documented research on urban forestry. Future research should address these underrepresented themes, as they are crucial for the long-term sustainability of urban forestry projects. Particularly, research on financing mechanisms is essential to unlock sustainable funding sources for urban forestry, a critical barrier to its expansion.

Preprint REVIEW | doi:10.20944/preprints202409.1391.v1

FGFR2 in the Development and Progression of Cutaneous Squamous Cell Cancer

Ethan M. Kallenberger, Alok Khandelwal, Priyatosh Nath, Shaun A. Nguyen, John DiGiovanni, Cherie-Ann O. Nathan

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cutaneous Squamous Cell Carcinoma; Fibroblast Growth Factor Receptor 2; Treatment Resistance; Epithelial Mesenchymal Transition; Metastasis; Immunosuppression

Online: 18 September 2024 (11:16:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1393.v1

Bioprospecting of Metsnikowia pulcherrima Strains, Isolated from the Vineyard Ecosystem, as Novel Starter Cultures for Craft Beer Production

Julian Karaulli, Nertil Xhaferaj, Francesca Coppola, Bruno Testa, Francesco Letizia, Onejda Kyçyk, Renata Kongoli, Mamica Ruci, Fatbardha Lamçe, Kapllan Sulaj, Massimo Iorizzo

Subject: Biology And Life Sciences, Food Science And Technology Keywords: craft beer; Metschnikowia pulcherrima; Saccharomyces cerevisiae; sequential inoculation

Online: 18 September 2024 (10:06:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1342.v1

Heat Stress in Japanese Quails (Coturnix coturnix japonica): Benefits of Phytase Supplementation

Apolônio Gomes Ribeiro, Raiane dos Santos Silva, Dayane Albuquerque da Silva, Júlio Cézar dos Santos Nascimento, Lilian Francisco Arantes de Souza, Edijanio Galdino da Silva, José Evangelista Santos Ribeiro, Danila Barreiro Campos, Clara Virgínia Batista de Vasconcelos Alves, Edilson Paes Saraiva, Fernando Guilherme Perazzo Costa, Ricardo Romão Guerra

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Antinutritional factors; Calcium absorption; Calbindin-D28K; Exogenous enzymes; TRPV6.

Online: 18 September 2024 (08:53:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1233.v1

Immobilization of Phospholipase D on Magnetic Graphene Oxide for Efficient Phosphatidylserine Production

Huiyi Shang, Bishan Guo, Juntan Wang, Huijuan Li, Haihua Zhu

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: phospholipase D; immobilization; phosphatidylserine

Online: 18 September 2024 (05:26:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1335.v1

Morphological Characterization, Polyphenolic Profile, and Bioactive Properties of Limoncella, an Ancient Mediterranean Variety of Sweet Citrus

Lucia Potenza, Roberta Saltarelli, Francesco Palma, Laura Di Patria, Sabrina Burattini, Giosuè Annibalini, Pietro Gobbi, Laura Valentini, Giovanni Caprioli, Agnese Santanatoglia, Sauro Vittori, Elena Barbieri

Subject: Biology And Life Sciences, Food Science And Technology Keywords: albedo; flavedo; bioactive compound; functional food

Online: 17 September 2024 (14:17:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1333.v1

Astrocyte Morphological Adaptations in a Mouse Model of Social Anxiety Disorder

Laura Boi, Rohit Menon, Barbara Di Benedetto

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Astrocytes, astrocyte morphology, social fear, lateral septum, mice

Online: 17 September 2024 (14:08:48 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1318.v1

Cortical Organotypic Brain Slice Cultures to Examine Sex- and Age-Dependent Astrocyte-Mediated Synaptic Phagocytosis

Anastasiia Nagumanova, Lea R. Seeholzer, Barbara Di Benedetto

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Organotypic brain slice culture; astrocyte; phagocytosis; synaptic elimination; prefrontal cortex; critical period; major depressive disorder

Online: 17 September 2024 (11:42:15 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1316.v1

The Utilization of PRAME in the Diagnosis, Prognosis, and Treatment of Melanoma

Samuel L Blount, Xiaochen Liu, Jeffrey Don McBride

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: melanoma; PRAME; cell biology; cancer; immunotherapy

Online: 17 September 2024 (11:39:00 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1289.v1

The Sixth Mass Extinction and Amphibian Species Sustainability Through Reproduction Biotechnologies, Biobanking, Species Restoration, and Conservation Breeding Programs

Robert K. Browne, Qinghua Lou, Pei Wang, Nabil Mansour, Svetlana A Kaurova, Edith N Gakhova, Natalia V Shishova, Victor K Uteshev, Lyudmilla I Kramarova, Govindappa Venu, Mikhail F Bagaturov, Somaye Vaissi, Pouria Heshmatzad, Peter Janzen, Aleona Swegen, Julie Strand, Dale McGinnity

Subject: Biology And Life Sciences, Life Sciences Keywords: Mass extinction; COP 15; COP 28; reproduction technologies; intergenerational justice; de-extinction; climate catastrophe; assisted evolution; terraforming; space colonisation

Online: 17 September 2024 (11:33:48 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1298.v1

Tackling Infectious Diseases in the Caribbean and South America: Epidemiological Insights, Antibiotic Resistance, and Immunological Advances

Angel Justiz-Vaillant, Darren Gopaul, Rodolfo Arrozarena-Fundora, Odalis Asin-Milan, Patrick Akpaka

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Tuberculosis; HIV; Nosocomial infections; Antibiotic resistance; Salmonella; Blood transfusion safety; Immunology; West Indies; Mycobacterium tuberculosis; Gram-negative bacteria; IgM antibodies

Online: 17 September 2024 (11:31:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1311.v1

An In Vitro Assay to Measure Astrocyte-Dependent Synaptic Phagocytosis in Health and Major Depressive Disorder

Diego Julian Vesga-Jimenez, Eugenia Vivi, Barbara Di Benedetto

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: Confocal microscopy; astrocytes; phagocytosis; prefrontal cortex; major depressive disorder; Synaptic pruning

Online: 17 September 2024 (10:53:17 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1271.v1

Exploring the Impact of Environmental Conditions and Bioreactors on Microalgae Growth and Applications

Sally Do, Zhi-Yan Du

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: bioreactors; photobioreactors; microalgae; cultivation factors; incubation factors

Online: 17 September 2024 (09:56:35 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1281.v1

The Intersection of Cancer Biomarkers and Superficial Acral Fibromyxoma: A Review of Literature for Pathologists and Dermatologists

Michelle Robert Anthony, Christopher Farkouh, Parsa Abdi, Faiza Amatul-Hadi, Amritpal Kooner, Conor Dolehide

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: superficial acral fibromyxoma; Rb 1 deletion; ICH; sclerosing perineuroma; dermatofibrosarcoma protuberans

Online: 17 September 2024 (08:45:20 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1280.v1

The Role of Copper in Alzheimer’s Disease Etiopathogenesis: An Updated Systematic Review

Angela Sabalic, Veronica Mei, Giuliana Solinas, Roberto Madeddu

Subject: Biology And Life Sciences, Toxicology Keywords: Alzheimer's disease; Copper; Ceruloplasmin; Neurotoxicity; Metals

Online: 17 September 2024 (08:43:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1278.v1

A Circle-Drawing Task for Studying Reward-Based Motor Learning in Children and Adults

Nina M. van Mastrigt, Jeroen B.J. Smeets, A. Moira van Leeuwen, Bernadette C.M. van Wijk, Katinka van der Kooij

Subject: Biology And Life Sciences, Behavioral Sciences Keywords: motor learning; adaptation; reward; development

Online: 17 September 2024 (08:43:32 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1268.v1

Is Water the Engine of Protein Folding?

Marco Campanile, Giuseppe Graziano

Subject: Biology And Life Sciences, Biophysics Keywords: water; globular proteins; folding; hydrophobic effect

Online: 17 September 2024 (08:16:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1166.v1

Comparative Study of a Compound Antioxidant and Ethoxyquin on Feed Oxidative Stability, Broiler Performance, Antioxidant Capacity, and Intestinal Health

Yong Xiao, Xuyang Gao, Jianmin Yuan

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: compound antioxidant; ethoxyquin; broiler; antioxidant capacity; intestinal health

Online: 17 September 2024 (04:59:06 CEST)

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Concerns regarding the safety of ethoxyquin (EQ) have gradually increased, highlighting the necessity of identifying safer and more effective antioxidants for use in animal feed. This study investigates the effects of a compound antioxidant (CA) as an alternative to ethoxyquin on feed oxidative stability, growth performance, antioxidant capacity, and intestinal health in broilers. A total of 351 one-day-old Arbor Acres Plus male broilers were randomly assigned to three treatments with nine replicates of 13 birds each. The experimental treatments included: CON group (basal diet), EQ group (basal diet + 200 g/ton EQ at 60% purity), and CA group (basal diet + 200 g/ton compound antioxidant, which comprises 18% butylated hydroxytoluene, 3% citric acid, and 1% tertiary butylhydroquinone). The experiment lasted for 21 days. Compared to the control group, supplementation with CA significantly reduced the acid value, peroxide value, and malondialdehyde content in the feeds after storage. Moreover, CA supplementation significantly decreased feed intake and the feed conversion ratio (FCR) without affecting weight gain of boilers, while also improving liver antioxidant capacity (increased T-AOC and SOD levels), intestinal antioxidant capacity (increased CAT, HO-1, and GPX7 protein expression), and enhancing the duodenal villus height-to-crypt depth ratio and jejunal villus height. Additionally, CA treatment reduced intestinal barrier permeability (decreased diamine oxidase activity and D-lactate levels), potentially by promoting the expression of Occludin, ZO-1, and Mucin2 protein. Furthermore, the addition of CA elevated the protein levels of NF-κB p50 along with its inhibitory factors, IκB and NF-κB p105, in the intestines of broilers, thereby enhancing immune regulatory capacity. This supplementation also led to an increased abundance of beneficial gut microorganisms, including Lactobacillus, Alistipes, Candidatus Arthromitus, and Turicibacter. In comparison to the EQ group, CA supplementation enhanced feed oxidative stability, decreased the FCR, and improved the intestinal morphology, barrier functions, and inflammatory status in broilers. These findings suggest that this compound antioxidant may serve as a healthier and more effective alternative to ethoxyquin.

Preprint REVIEW | doi:10.20944/preprints202409.1191.v1

G4 Biology and Scope for Potential Therapeutic Intervention against Multiple Disease Adversaries

Saleem Y Bhat

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: replication; G4 DNA; helicases; Drug discovery

Online: 17 September 2024 (04:57:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1263.v1

CFD Simulation of Dynamic Temperature Variations Induced by Tunnel Ventilation in a Broiler House

Lak-yeong Choi, Kehinde Favour Daniel, Se-yeon Lee, Chae-rin Lee, Ji-yeon Park, Jinseon Park, and Se-woon Hong

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Poultry heat production; thermal gradients; microclimate; inlet baffles; CFD validation

Online: 17 September 2024 (00:41:41 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1260.v1

Solanum indicum Linn.: Bridging the Gap Between Traditional Knowledge and Modern Scientific Discoveries

Ritu Yadav, Shashank Tiwari

Subject: Biology And Life Sciences, Life Sciences Keywords: Indian Nightshade, Phytochemistry, Pharmacological properties, Traditional medicine, Antioxidant activity, Anti-inflammatory activity, Anticancer activity, Antimicrobial activity, Analgesic activity, Antipyretic activity, Antidiabetic activity

Online: 16 September 2024 (15:29:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1248.v1

Antifungal Activity of Citrus Essential Oil in Controlling Sour Rot in Tahiti Acid Lime Fruits

Vanessa Santos Moura, Lara Dias Orlandin, Beatriz Saraiva Mariano, Josiane Rodrigues, Fernando Trevizan Devite, FERNANDO ALVES DE AZEVEDO, Marines Bastianel

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: natural fungicides; post-harvest; biocontrol; agricultural sutainaibility

Online: 16 September 2024 (14:35:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1238.v1

Placental Homeostasis of Oxytocin and Its Ability to Affect the Biological Activity of Retinal Cells

Claudette Adegboro, Wenxiang Luo, Meha Kabra, Ryan M. McAdams, Nathaniel York, R I Wijenayake, Kianna Mary Suchla, De-Ann M Pillers, Bikash Ranjan Pattnaik

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: Preterm; retinal vascularization; retinopathy; transcriptomic; connectivity; OXT

Online: 16 September 2024 (14:09:42 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1179.v1

Dysregulated Expression of Selected miRNAs in Human Papillomavirus-Positive Patients with Benign Prostatic Hyperplasia/Prostatitis and Prostate Cancer

Sandra Viridiana Salgado-Hernández, Lucero Martínez-Retamoza, Rodolfo Ocadiz-Delgado, Salvador Pérez-Mora, Gladys Cedeño-Arboleda, María del Consuelo Gómez-García, Patricio Gariglio, David Guillermo Pérez-Ishiwara

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: HPV; prostate cancer; BPH; prostatitis; miRNAs; inflammation; proliferation; apoptosis; angiogenesis; metastasis

Online: 16 September 2024 (12:29:00 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202409.1226.v1

Novel Compound Nonsense Variant in the CYP27B1 Cause an Atypical Form of Vitamin D-Dependent Rickets Type 1A: Case Report in Two Siblings in a Mexican Family

Jaime Toral López, Cesar Candia Tenopala, Daniela Reyes Mosqueda, Miguel Ángel Fonseca Sánchez, Luz María González Huerta

Subject: Biology And Life Sciences, Life Sciences Keywords: Vitamin D-deficient rickets type 1A; CYP27B1 gene; compound nonsense heterozygous; hypophosphatemia; atypical; calcitriol

Online: 16 September 2024 (12:28:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1147.v1

The Metallothionein System in Tetrahymena Thermophila Is Iron Inducible

Davide Gualandris, Davide Rotondo, Candida Lorusso, Antonio Calisi, Francesco Dondero

Subject: Biology And Life Sciences, Ecology, Evolution, Behavior And Systematics Keywords: aquatic system; heavy metals; evolution; oxidative stress; pollution; protist; iron homeostasis

Online: 16 September 2024 (12:20:45 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202409.1189.v1

Comparisons of Distribution of CpG Islands Through the Sex-Chromosomes Between Rat (Rattus norvegicus) and Swine (Sus scrofa)

Shilpa Tewari

Subject: Biology And Life Sciences, Life Sciences Keywords: CpG island; in silico prediction; X-chromosome; Y-chromosome

Online: 16 September 2024 (10:19:05 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1184.v1

Patho-Ecological Distribution and Genetic Diversity of Fusarium oxysporum f. sp. cubense in Malbhog Banana Belts of India

Anisha Baruah, Popy Bora, T. Damodaran, Bishal Saikia, Muthukumar. M., Prakash Patil, Ashok Kumar Bhattacharyya, Ankita Saikia, Alok Kumar, Sangeeta Kumari, Juri Talukdar, Utpal Dey, Shenaz Sultana Ahmed, Naseema Rahman, Bharat Chandra Nath, Ruthy Tabing, Sandeep Kumar

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Fusarium wilt; Malbhog banana; India; Diversity analysis; Molecular characterization; Polymorphism; Race; AMOVA

Online: 16 September 2024 (10:17:02 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1186.v1

The Curtain Model as an Alternative and Complementary to the Classic Turgor Concept of Filamentous Fungi

Igor Mazheika, Olga Kamzolkina

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: basidiomycetes; the cell wall; plasma membrane adhesion and tension; F-actin; plasma membrane macroinvaginations; lomasomes

Online: 16 September 2024 (10:14:57 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1202.v1

Interplay Between the Circadian Clock and Sirtuins

Yan Zhuang, Yantong Zhang, Chao Liu, Yingbin Zhong

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: circadian clock; circadian rhythm; clock gene; sirtuin; metabolism

Online: 16 September 2024 (09:59:47 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1194.v1

Molecular Mechanism of Processing Ubiquitinated Substrates by p97 and Its Main Cofactors

Abolfazl Mirzadeh, Mohsen Kazemi, Isabelle Rouiller

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: p97; cofactors; substrate processing; Single particle cryo_electron microscopy

Online: 16 September 2024 (09:59:19 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1162.v1

Digital Magnetic Sorting for Fractionating Cell Populations with Variable Antigen Expression in Cell Therapy Process Development

Savannah Bshara-Corson, Andrew Burwell, Timothy Tiemann, Coleman Murray

Subject: Biology And Life Sciences, Life Sciences Keywords: Cell Therapy Manufacturing; Digital Magnetic Sorting; Antigen Density

Online: 16 September 2024 (09:54:30 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1151.v1

Whole-Genome Sequencing Analysis of Antimicrobial Resistance, Virulence Factors, and Genetic Diversity of Salmonella Isolated from Wenzhou, China

Yafang Jin, Yi Li, Shaojie Huang, Chengji Hong, Xucong Feng, Huidi Cai, Yanmei Xia, Shengkai Li, Leyi Zhang, Yongliang Lou, Wanchun Guan

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Salmonella; antimicrobial resistance; pathogenicity islands; cgMLST; Whole genome sequencing

Online: 16 September 2024 (03:20:34 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1130.v1

Global Trends in Disease Burden, Morbidity, and Mortality: Implications of Emerging Artificial Intelligence (AI) Technologies

Richard Murdoch Montgomery

Subject: Biology And Life Sciences, Life Sciences Keywords: Disease Burden; NCD; Emerging AI

Online: 15 September 2024 (07:32:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1115.v1

Combined Expression of DNMT3B and PFKFB4 in Hepatoblastoma Predicts Metastatic Outcome

Christophe Desterke, Raquel Francés, Claudia Monge, Agnès Marchio, Pascal Pineau, Jorge Mata-Garrido

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Hepatoblastoma; Metastasis; CHIC Risk; Metabolism; Epigenetics; DNA Methylation; Glycolysis; Transcriptome

Online: 15 September 2024 (07:32:32 CEST)

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(1) Background: Hepatoblastoma is the most common primary liver cancer in children. Nowadays, poor outcome occurs essentially for patients with distant metastases; (2) Methods: Starting from Mammalian Metabolic Enzyme Database over expression of metabolic enzymes was searched in hepatoblastoma tumors as compared to noncancerous liver tissue in transcriptome dataset GSE131329. With overexpressed enzymes, Elasticnet machine learning tuning was performed according to metastasis status outcome. With significant enzymes a metabolic expression score was computed and integrated in multivariate clinical-biological logistic model; (3) Results: Forty-one overexpressed enzymes discriminate hepatoblastoma tumors and noncancerous liver tissues. Eighteen of them predict metastasis status (AUC 0.90) with 85.7% of sensibility and 92.3% of specificity. Elasticnet machine learning model tuning highlighted major importance of DNMT3B and PFKFB4 expression to predict metastasis. Role of these two enzymes was confirmed by univariate analyses with respective p-values: 0.0058 and 0.0091. Metabolic score computed with combined expression of DNMT3B and PFKFB4 discriminates metastasis status and high-risk CHIC score by univariate analysis (p-value=0.005). Meta.score was found more sensitive than C1/C2 classifier to predict metastasis status (accuracy: 0.72 versus 0.55). Integration of meta.score (DNMT3B, PFKFB4) with epidemiological parameters: gender, age at diagnosis, histological types, and clinical PRETEXT stages in a multivariate model confirmed independent adverse role of meta.score to predict metastasis status (multivariate p-value=0.003, odds ratio: 2.12); (4) Conclusions: Based on metabolic enzyme expression program of hepatoblastoma, we characterized a dual overexpression of PFKFB4 and DNMT3B in samples of patients at risk of metastasis (High risk CHIC stratification). With combined tumor expression of DNMT3B and PFKFB4 a meta.score was computed and this parameter was confirmed as an independent adverse score to predict metastatic status during hepatoblastoma.

Preprint ARTICLE | doi:10.20944/preprints202409.1112.v1

Genetic Variation Study of Several Romanian Pepper (Capsicum annuum L.) Varieties Revealed by Molecular Markers and Whole Genome Resequencing

Anca Amalia UDRIȘTE, Mihaela IORDĂCHESCU, Liliana BĂDULESCU

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Capsicum annuum; south-east Romania; molecular markers; Sanger sequencing; Whole Genome Resequencing

Online: 15 September 2024 (07:32:20 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1123.v1

Transcriptomic Profiling Reveals Altered Expression of Genes Involved in Metabolic and Immune Processes in NDV-Infected Chicken Embryos

Malarmathi Muthusamy, Kannaki T Ramasamy, Sunday Olusola Peters, Srinivasan Palani, Gowthaman Vasudevan, Murali Nagarajan, Sivakumar .Karuppusamy, Vasanthakumar Thangavelu, Thiruvenkadan Aranganoor kannan

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: NDV; Chicken; Aseel; Kadaknath; White leghorn; Immune gene; Metabolic genes; HSP

Online: 15 September 2024 (07:32:16 CEST)

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Preprint CASE REPORT | doi:10.20944/preprints202409.1137.v1

Exploring Dengue Infection in a Vaccinated Individual: Preliminary Molecular Diagnosis and Sequencing Insights

Talita Émile Ribeiro Adelino, Sílvia Helena Sousa Pietra Pedroso, Maurício Lima, Luiz Marcelo Ribeiro Tomé, Natália Rocha Guimarães, Vagner Fonseca, Paulo Eduardo de Souza da Silva, Keldenn Melo Farias Moreno, Ana Cândida Araújo e Silva, Náthale Rodrigues Pinheiro, Carolina Senra Alves de Souza, Luiz Carlos Junior Alcantara, Marta Giovanetti, Felipe Campos de Melo Iani

Subject: Biology And Life Sciences, Virology Keywords: DENV monitoring; next-generation sequencing; genomic surveillance; vaccine breakthrough

Online: 14 September 2024 (11:46:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1110.v1

How Accurately Topsoil Texture Is Shown on Agricultural Soil Maps? A Case Study of Eleven Fields Located in Poland

Michał Stępień, Dariusz Gozdowski, Stanisław Samborski

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: agricultural soil maps; soil texture class; agronomic category; purity; averaged agreement

Online: 14 September 2024 (10:13:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1060.v1

Evaluation of the Antifungal Activity of Eugenol against the Candida albicans Genomic DNA

Danyelle Gomes de Sousa, Francisca Alves dos Santos, Dárcio Luiz de Sousa Júnior, Tássia Thaís Al Yafawi, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, Maria Karollyna do Nascimento Silva Leandro

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: cell death; eugenol; fungal DNA

Online: 14 September 2024 (10:08:55 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1114.v1

Assessing the Pathogenicity of Berkeleyomyces rouxiae and Fusarium oxysporum f. sp. vasinfectum on Cotton (Gossypium hirsutum) Using a Rapid and Robust Seedling Screening Method

Andrew Chen, Duy P. Le, Linda J. Smith, Dinesh Kafle, Elizabeth A. B. Aitken, Donald M. Gardiner

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Upland cotton; pathogenicity; fungal effectors; Fusarium wilt; black root rot; Secreted In Xylem effector genes; endophyte; Translation elongation factor-1 alpha subunit

Online: 14 September 2024 (08:49:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1116.v1

Characterization of Biofilm Formation by Staphylococcus aureus Depending on NaCl Concentration

Yusuke Iwabuchi, Hitoyuki Kato, Masanori Saito, Hidenobu Senpuku

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: biofilm; membrane vesicle; S. mutans; NaCl; S. aureus

Online: 14 September 2024 (08:44:10 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1109.v1

Intelligibility Sound Therapy Enhances the Ability of Speech-in-Noise Perception and Pre-perceptual Neurophysiological Response

Takashi Ishino, Kei Nakagawa, Fumiko Higashikawa, Sakura Hirokane, Rikuto Fujita, Chie Ishikawa, Tomohiro Kawasumi, Kota Takemoto, Takashi Oda, Manabu Nishida, Yuichiro Horibe, Nobuyuki Chikuie, Takayuki Taruya, Takao Hamamoto, Tsutomu Ueda, Louis Yuge, Sachio Takeno

Subject: Biology And Life Sciences, Life Sciences Keywords: intelligible-hearing (IH) sound; sensory neural hearing loss; speech-in-noise perception; cortical auditory evoked fields (AEF); N1m-P2m amplitude; magnetic mismatch negativity (MMNm)

Online: 14 September 2024 (07:31:09 CEST)

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Aural rehabilitation with hearing aids can decrease the attentional requirements of cognitive resources by amplifying the deteriorated-frequency sound in hearing loss patients and improve the auditory discrimination ability like speech-in-noise perception. As aural rehabilitation with an intelligible-hearing sound also can be hopeful, the aim of this study was to evaluate the effectiveness of the aural rehabilitation with intelligible-hearing sound for hearing loss patients. Adult native Japanese speakers (17 males and 23 females, 68.43±9.23 years) showed over 30 dB at 125, 250, 500, 1000, 2000, 3000, 4000, 8000, 10000, and 12000Hz on any side, were recruited and underwent Mini-Mental State Examination Japanese. we conducted self-evaluation questionnaire for hearing problems of voice, gap detection test, fast speech test, and speech-in-noise test, pure tone audiogram, speech perception test using Japanese 67-S, cortical auditory evoked fields, and magnetic mismatch negativity before and after the non-intelligible-hearing (N=20) and intelligible-hearing (N=20) sound therapy with listening to music for one hour a day for 35 days. Better hearing ear was defined using a four-frequency pure-tone average at the thresholds of 500, 1000, 2000 and 4000 Hz. After the sound therapy, speech-in-noise test with signal to noise ratio +10 in better hearing ear showed significant improvement (p<0.05), and N1m-P2m amplitudes showed the significant increase in Lt superior temporal gyrus at the stimulus from better hearing ear (p<0.05). The significant enhancement of the magnetic mismatch negativity amplitude at Lt superior temporal gyrus was exhibited after the sound therapy (p<0.01). Intelligible-hearing sound therapy can improve the ability of speech-in-noise perception in better hearing ear and the enhancement of central cortex response, which reflect the ability of working memory, was proved by cortical auditory evoked fields and magnetic mismatch negativity. Intelligible-hearing sound therapy can be a valuable aural rehabilitation method for sensory neural hearing loss as same as hearing aids.

Preprint ARTICLE | doi:10.20944/preprints202409.1105.v1

Urban Ecology of Red-Whiskered Bulbul: Feeding Behavior, Breeding Success and Behavioural Patterns in New Barrackpore, West Bengal, India

Md. Abu Imran Mallick, Nirdesha Biswas

Subject: Biology And Life Sciences, Behavioral Sciences Keywords: bird species; behavioral adaption; conservation; foraging strategies; habitat adaption; Pycnonotus jocosus

Online: 14 September 2024 (05:39:01 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1020.v1

The Effects of Competition on Exercise Intensity and the User Experience of Exercise, during Virtual Reality Bicycling for Young Adults

John L Palmieri, Judith E Deutsch

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Virtual reality; bicycling; aerobic exercise; wearable sensors; visual feedback; competition; eye-tracking; visual attention; motivation; enjoyment

Online: 14 September 2024 (05:29:07 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1025.v1

Diversity and Evolution of NLR Genes in Citrus Species

Zhiwei Xiong, Wanshan Zhang, Hui Yin, Jiaxing Wan, Zhuozhuo Wu, Yuxia Gao

Subject: Biology And Life Sciences, Horticulture Keywords: NLR genes; citrus; diversity; adaptive evolution

Online: 14 September 2024 (05:26:51 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1106.v1

Optimized Polyhydroxybutyrate Production by Neobacillus Niacinii GS1 Utilizing Corn Flour, Wheat Bran, and Peptone: A Sustainable Approach

Shrimali Gaurav, Gangawane Ajit, Rami Esha, Shah Hardik, Thumar Kashyap, Deepak Sahoo, Ashish Patel, Jens Ejbye Schmidt

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: Polyhydroxybutyrate (PHB); Neobacillus niacinii GS1; Response Surface Methodology (RSM); Biodegradable Plastics; Agricultural Residual; Biopolymer Production

Online: 14 September 2024 (05:25:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1017.v1

Optimal Forest Management Using Multi-Objective and Game Theory Techniques: Considering Environmental and Economic Factors

Neda Amiri, Soleiman Mohammadi Limaei

Subject: Biology And Life Sciences, Forestry Keywords: decision making; multi-objective; Pareto optimum; game theory; Nash equilibrium; epsilon-constrain; net present value; carbon sequestration

Online: 14 September 2024 (05:00:25 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1090.v1

Pacemaker Optimization Mechanisms in the Spectrum of Cardiac Disease Rationale to Protocol

Philip Houck

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: pacemaker optimization; protocol for spectrum of cardiac disease; Echocardiogram parameters

Online: 14 September 2024 (04:34:43 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1016.v1

DNA Gene's Basic Structure as a Nonperturbative Circuit Quantum Electrodynamics: Is RNA Polymerase II the Quantum Bus of Transcription?

Raul Riera Aroche, Yveth M. Ortiz García, Esli C. Sánchez Moreno, José S. Enriquez Cervantes, Andrea C. Machado Sulbaran, Annie Riera Leal

Subject: Biology And Life Sciences, Biophysics Keywords: DNA gene's basic structure; circuit quantum electrodynamics; quantum bus of transcription

Online: 14 September 2024 (03:36:16 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1057.v1

Improving Crops Using Omics Databases of Arabidopsis (Arabidopsis Thaliana) and Other Model Plants

Andrés S. Ortiz-Morazán, Marcela María Moncada

Subject: Biology And Life Sciences, Plant Sciences Keywords: Arabidopsis (Arabidopsis thaliana), Bioinformatics, Genetic improvement, Omics databases, Crops.

Online: 13 September 2024 (14:51:44 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1037.v1

Co-extraction of DNA and RNA from Candida albicans Using a Chemical Method in Conjunction with Silicon Carbide with Few Cells

Elizabeth Cristina Vieira de Freitas, Francisca Alves dos Santos, Maria Raíssa Vieira Lopes, Dárcio Luiz de Sousa Júnior, Tássia Thaís Al Yafawi, Ana Carolina Ferreira Araújo, Priscilla Ramos Freitas, Irwin Rose Alencar de Menezes, Henrique Douglas Melo Coutinho, Maria Karollyna do Nascimento Silva Leandro

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Chloriform-phenol; Extraction; Nucleic acids

Online: 13 September 2024 (11:32:13 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1082.v1

Effect of Temperature Over the Growth and Biofilm Formation of the Thermotolerant Aspergillus flavus

José Alejandro Hernández-Benítez, Brenda Nallely Santos-Ocampo, Daniel Genaro Rosas-Ramírez, Adrian Ramírez-Granillo, Luis Antonio Bautista-Hernández, Víctor Manuel Bautista-de Lucio, Nestor Octavio Pérez, Aída Verónica Rodríguez-Tovar

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: Aspergillus flavus; biofilm; thermotolerant; extracellular matrix; lipid droplets; antifungal susceptibility

Online: 13 September 2024 (11:25:49 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1039.v1

Dereplication of Tetronic Acid Derivatives Produced by Calea pinnatifida (Asteraceae) Endophytes and Their Antimicrobial Properties

Bianca Barna, Lhaís Araújo Caldas, Jackson Monteiro, Augusto Leonardo Santos, Renata Castiglioni Pascon, Marcelo Afonso Vallim, Marcelo José Pena Ferreira, Sarah Santos Gonçalves, Glaucia Queiroz dos Santos, Anderson Messias Rodrigues, Jamile Ambrósio de Carvalho, Suzan Pantaroto de Vasconcellos, Patricia Sartorelli

Subject: Biology And Life Sciences, Life Sciences Keywords: Calea pinnatifida, Endophytic fungi, Hypomontagnella barbarensis, antimicrobial activity, dereplication.

Online: 13 September 2024 (10:43:26 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1066.v1

Nitrogen Assimilation, Biomass and Yield in Response to Application of Algal Extracts, Rhizobium sp. and Trichoderma asperellum, as Biofertilizers in Hybrid Maize

Sandra Pérez-Álvarez, Erick H. Ochoa-Chaparro, Julio César Anchondo-Páez, César M. Escobedo-Bonilla, Joel Rascón-Solano, Marco A. Magallanes-Tapia, Luisa Patricia Uranga-Valencia, Reinier Hernández-Campos, Esteban Sánchez

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: algal extracts; biofertilizers; nitrogen assimilation; Rhizobium sp.; Trichoderma asperellum; Zea mays L.

Online: 13 September 2024 (08:46:08 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.1049.v1

Aging of the Neuromuscular System: What Are the Causes of Age-Related Loss of Strength and Muscle Mass, and Can We Prevent Its Development and Progression?

Thomas Gustafsson, Brun Ulfhake

Subject: Biology And Life Sciences, Aging Keywords: dynapenia; muscle fiber atrophy; motor unit; ageing; senescence

Online: 13 September 2024 (08:25:06 CEST)

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Preprint CONCEPT PAPER | doi:10.20944/preprints202409.1063.v1

Fractal Calculus Facilitates Rethinking 'Hard Problems'; A New Research Paradigm

BRUCE West

Subject: Biology And Life Sciences, Other Keywords: Fractal Paradigm; fractal calculus; heart rate variability (HRV); Levy statistics; control hypothesis; sinoatrial node control

Online: 13 September 2024 (08:24:23 CEST)

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Preprint REVIEW | doi:10.20944/preprints202406.0714.v3

Analysis of Beyfortus® (Nirsevimab) Immunization Campaign: Effectiveness, Biases, and ADE Risks in RSV Prevention

Helene Banoun

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: nirsevimab; beyfortus; ADE; RSV; bronchiolitis; mAbs; therapeutic monoclonal antibody

Online: 13 September 2024 (08:24:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1051.v1

Antioxidant Activity of Zingiber Officinale R. Extract Using Pressurized Liquid Extraction Method

Marlon Saldaña-Olguin, Bernardo Junior Quispe-Ciudad, Elza Berta Aguirre-Vargas

Subject: Biology And Life Sciences, Food Science And Technology Keywords: antioxidant activity; aqueous extract; ginger; phenolic compounds; pressurized liquid extraction

Online: 13 September 2024 (04:23:54 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0951.v1

The Emerging Role of Long Noncoding RNAs’ in Sorafenib Resistance within Hepatocellular Carcinoma

Puneet Vij, Mohammad Shabir Hussain, Sanjaya Kumar Satapathy, Everardo Cobos, Manish Kumar Tripathi

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Long noncoding RNA (LncRNA); Sorafenib Resistance; Hepatocellular Carcinoma (HCC); EGFR; Autophagy; VEGFA; Proteomics; Renal Cell Carcinoma (RCC)

Online: 13 September 2024 (04:17:59 CEST)

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Preprint REVIEW | doi:10.20944/preprints202407.0680.v2

A Systematic Review of Aphantasia: Concept, Measurement, Neural Basis, and Theory Development

Feiyang Jin, Shen-Mou Hsu, Yu Li

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: aphantasia; visual imagery; mental imagery; vividness; cognitive functioning; mental health

Online: 12 September 2024 (16:01:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1006.v1

Effect of De-Husking on Nutritional and Antioxidant Quality of Nigerian Millet Varieties

Abduljalil Muhammad Mode, Umar Faruk Magaji, Salisu Yusuf Yahaya, Nuhu Ginom Zaki, Shamsudeen Umar Dandare

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Millet; De-husking; Antioxidants; Phytochemicals; Minerals; Vitamins

Online: 12 September 2024 (15:43:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0997.v1

Chemical Markers in Italian Propolis: Chrysin, Galangin and Cape as Indicators of Geographic Origin

Elisabetta Miraldi, Giorgio Cappellucci, Giulia Baini, Elia Silvia Pistone, Marika Allodi, Gabriele Costantino, Chiara Spaggiari, Marco Biagi

Subject: Biology And Life Sciences, Food Science And Technology Keywords: Italian propolis; flavonoids; pinocembrin; chrysin; CAPE; geographical diversity.

Online: 12 September 2024 (15:41:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.1030.v1

Unveiling the Nutrient Signatures in Corn (Zea Mays L.) Grains: A Pivotal Indicator of Yield Potential

Nour Ismail, Lotfi Khiari, and Rachid Daoud

Subject: Biology And Life Sciences, Agricultural Science And Agronomy Keywords: Nutrient Diagnosis Norms (NDN), Nutrient signature, Global nutrient imbalance index (GNII), High yielding subpopulation

Online: 12 September 2024 (15:41:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0928.v1

Two Forms of Thick Filament in the Flight Muscle of Drosophila melanogaster

Hosna Rastegarpouyani, Alimohammad Hojjatian, Kenneth A. Taylor

Subject: Biology And Life Sciences, Biophysics Keywords: ageing; flightin; myofilin; paramyosin; stretchin-klp

Online: 12 September 2024 (15:29:31 CEST)

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Preprint BRIEF REPORT | doi:10.20944/preprints202409.0995.v1

Knee Joint Response to Mechanical Loading: Bounding Mechanotransduction with Rehabilitation

Bartłomiej Kacprzak, Mikołaj Stańczak

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: knee joint; molecular biology; cell biology; mechanotransduction

Online: 12 September 2024 (12:56:41 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0922.v1

Biofilm Formation and Anti-Inflammatory Response of a Probiotic Blend

Nicholas L.F. Gallina, Nicole I Tardi, Xilin Li, Alvin Cai, Mandy Horn, Bruce Applegate, Lavanya Reddivari, Arun K. Bhunia

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Probiotics; biofilm; antimicrobial activity; adhesion; pathogens; canine gut health; anti-inflammatory response

Online: 12 September 2024 (11:26:45 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0933.v1

Congenital Radioulnar Synostosis Review: Recommendations and Treatment Outcomes

Sergi Alabau-Rodríguez, Jose Felix Garrido Ferrer, Xavier Mir-Bulló, Lidia Ana Martin Dominguez, Albert Pardo Pol, Francisco Soldado Carrera

Subject: Biology And Life Sciences, Life Sciences Keywords: radioulnar synostosis; radius; ulna; congenital

Online: 12 September 2024 (11:12:59 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0964.v1

Roseto Revisited: Fact or Fiction

Samrachana Adhikari, Olugbenga Ogedegbe, Orrin Devinsky

Subject: Biology And Life Sciences, Life Sciences Keywords: diet; obesity; health; social support; saturated fat

Online: 12 September 2024 (11:07:53 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0996.v1

Effect of Citrus Fruit Constructions on Gas Diffusion Resistance and Internal Gas Concentration of Oxygen and Carbon Dioxide for Various Cultivars of Citrus Fruits

Morimatsu Kazuya, Konagaya Keiji

Subject: Biology And Life Sciences, Horticulture Keywords: citrus fruit; gas diffusion resistance; respiration; internal gas concentration

Online: 12 September 2024 (10:57:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0926.v1

Genome Sequencing Reveals the Potential of Enterobacter sp. strain UNJFSC003 for Hydrocarbon Bioremediation

Gianmarco Castillo, Sergio Eduardo Contreras-Liza, Carlos I. Arbizu, Pedro Manuel Rodriguez-Grados

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: NGS; genomics; microbiology; benzo[a]pyrene; hydrocarbon and bioremediation; Molecular Docking

Online: 12 September 2024 (10:49:15 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0987.v1

3D FEM Modeling of Polish Apple ‘Chopin’: Elastic vs. Elastoplastic Behavior Across Maturity Stages

Monika Słupska, Roman Stopa

Subject: Biology And Life Sciences, Food Science And Technology Keywords: discrete model; FEM; apple; fruit; stage of ripening

Online: 12 September 2024 (09:23:08 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0984.v1

Development of a Sensitive and Reliable Immunoassay to Quantify TDP-43 in Human Biofluids

Jiyan An, Lathika Gopalakrishnan, Vanessa Ortega, Justin Saul, Renu Kadali, Robert Bowser

Subject: Biology And Life Sciences, Neuroscience And Neurology Keywords: TDP-43; immunoassay; MSD; ALS; biomarker; neurodegenerative disease

Online: 12 September 2024 (09:16:06 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0954.v1

Characterization of MSC Growth, Differentiation, and EV Production in CNF Hydrogels under Static and Dynamic Cultures in Hypoxic and Normoxic Conditions

Ilias Nikolits, Farhad Chariyev-Prinz, Dominik Egger, Falk Liebner, Nicolas Mytzka, Cornelia Kasper

Subject: Biology And Life Sciences, Biology And Biotechnology Keywords: mesenchymal stem cells; cellulose; hydrogels; hypoxia; extracellular vesicles; immunomodulation

Online: 12 September 2024 (08:18:03 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0958.v1

Assessment of Bacteriological Quality of Drinking Water in Taiz and Ibb Governorates, Yemen

Ali Ahmed Al-Hadheq, Wadhah Edrees, Bashir Al-Ofairi

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: Escherichia coli; ; Coliform; Total Bacterial Count; Presumptive Test; Taiz; Ibb; Yemen

Online: 12 September 2024 (08:16:18 CEST)

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Preprint REVIEW | doi:10.20944/preprints202409.0914.v1

Timing and Graded BMP Signalling Determines Fate of Neural Crest and Ectodermal Placode Derivatives from Pluripotent Stem Cells

Keshi Chung, Malvina Millet, Ludivine Rouillon, and Azel Zine

Subject: Biology And Life Sciences, Cell And Developmental Biology Keywords: bone morphogenetic proteins; human pluripotent stem cells; human cell models; organoids; preplacodal ectoderm; otic lineage

Online: 12 September 2024 (07:41:12 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0963.v1

CBD-X Extracts in Asthma Management: Reducing Th2-Driven Cytokine Secretion and Neutrophil/Eosonophil Activity

Miran Aswad, Antonina Pechkovsky, Narmeen Ghanayiem, Yaniv Dotan, Igal Louria-Hayon

Subject: Biology And Life Sciences, Immunology And Microbiology Keywords: CBD extracts; asthma; type 2 helper T cells (Th2); neutrophil; Eosonophil; OVA-Induced Asthmatic mouse model; cytokines; migration; IgE

Online: 12 September 2024 (05:35:21 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0935.v1

Changes in Gut Microbiota in a Peruvian Cattle Genetic Nucleus by Breed and Correlations with Beef Quality

Carlos Quilcate, Richard Estrada, Yolanda Romero, Diorman Rojas, Rolando Mamani, Renán Dilton Hañari-Quispe, Mery Aliaga, Walter Galindo, Héctor V. Vásquez, Jorge L. Maicelo, Carlos I. Arbizu

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: gut microbiota; cattle breeds; microbial diversity; meat quality; hematological parameters; host genetics; 16S/18S rRNA

Online: 12 September 2024 (03:28:35 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0870.v1

Prenatal Exposure to a Human Relevant Mixture of EDCs Affects Mandibular Development in Mice

Vagelis Rinotas, Antonios Stamatakis, Athanasios Stergiopoulos, Carl-Gustaf Bornehag, Joëlle Rüegg, Marietta Armaka, Efthymia Kitraki

Subject: Biology And Life Sciences, Endocrinology And Metabolism Keywords: EDCs; mandible; mice; micro-CT

Online: 11 September 2024 (17:08:57 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0859.v1

Comparing the Performance of Automatic Milking Systems through Dynamic Testing Also Helps to Identify Potential Risk Factors for Mastitis

Stefano Milanesi, Dario Donina, Viviana Chierici Guido, Francesca Zaghen, Valerio Massimo Sora, Alfonso Zecconi

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: milking machine; automatic milking system; milking dynamic test; bimodality; mastitis

Online: 11 September 2024 (16:59:22 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0907.v1

PIM Kinases Regulate Estrogen Receptor α Signaling in Luminal A Breast Cancer Cells

William B. Eccleshall, Kwan Long Mung, Iida-Maria Laiho, Cecilia M. Sahlgren, Päivi J. Koskinen

Subject: Biology And Life Sciences, Life Sciences Keywords: PIM kinases; estrogen receptor α; luminal A breast cancer; targeted therapy resistance

Online: 11 September 2024 (16:23:00 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0901.v1

Comprehensive Phylogenetic and Immunogenic Analysis of SARS-CoV-2 and Related Coronaviruses

Emmanuel Owusu Ansah, Kwadwo Fosu, Theophilus Abonyi Mensah, Emmanuel Plas Otwe, Daniel Sakyi Agyirifo

Subject: Biology And Life Sciences, Virology Keywords: Coronavirus; SARS-CoV-2; spike protein; phylogenetics; CTL epitope; antigenic

Online: 11 September 2024 (15:28:29 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0912.v1

Ellagic Acid Mitigates Cadmium Exposure-Induced Apoptosis in HT22 Cells via Inhibiting Oxidative Stress, Mitochondrial Dysfunction, and Activating Nrf2/Ho-1 Pathway

Yue Liu, Chunhong Chen, Zhihui Hao, Jianzhong Shen, Shusheng Tang, Chongshan Dai

Subject: Biology And Life Sciences, Biochemistry And Molecular Biology Keywords: Cadmium; Ellagic acid; JNK pathway; Oxidative stress; Apoptosis; Nrf2/HO-1 pathway

Online: 11 September 2024 (14:12:04 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0825.v1

Ripening Stage in Mechanical Properties of Chopin Apples for FEM Model Construction

Monika Słupska, Roman Stopa

Subject: Biology And Life Sciences, Food Science And Technology Keywords: surface pressure; Micro-Ct; flesh cracking; model validation; fruit

Online: 11 September 2024 (12:34:47 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0833.v1

Exploring Lignin Biosynthesis Genes in Rice: Evolution, Functions, and Expressions

Munsif Ali Shad, Xukai Li, Muhammad Junaid Rao, Zixuan Luo, Xianlong Li, Aamir Ali, Lingqiang Wang

Subject: Biology And Life Sciences, Plant Sciences Keywords: rice; lignin monomers; gene families; cell wall; gene expression

Online: 11 September 2024 (12:32:37 CEST)

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Preprint ARTICLE | doi:10.20944/preprints202409.0892.v1

Revisiting Avian Metapneumovirus Subtype B in Broiler Chickens and Turkeys in Morocco: First Molecular Characterization

Amine Mernizi, Abdeljelil Ghram, Hicham Fathi, Enrique Carceller Sebastiá, Juan-Luis Criado, Martina Dardi, Oumayma Arbani, Mohammed Bouslikhane, Mohammed Mouahid, Caterina Lupini, Elena Catelli, Siham Fellahi, Saadia Nassik

Subject: Biology And Life Sciences, Animal Science, Veterinary Science And Zoology Keywords: Avian Metapneumovirus; Subtype B; Morocco; characterization; sequencing; phylogenetic analysis

Online: 11 September 2024 (11:24:14 CEST)

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