Porcine Skin–Derived Reconstituted Lipid Nanoparticles Intrinsically Boost Fibroblast Proliferation and Migration

Chronic and hard-to-heal wounds remain a major clinical burden, yet many synthetic nanocarriers used in advanced dressings are constrained by limited biomimicry and concerns over inflammatory risk and off-target toxicity. Here we report porcine skin–derived reconstituted lipid nanoparticles (PS-rLNPs) as a tissue-origin, composition-preserving nanoplatform for wound repair. Total lipids extracted from fresh porcine skin were assembled into nanoparticles via a facile solvent-injection process. Lipidomics revealed a triglyceride- and phosphatidylcholine-dominant composition accompanied by minor membrane-relevant lipid species, suggesting a biocompatible, multi-lipid milieu. PS-rLNPs formed a stable nanoscale dispersion and maintained colloidal stability in physiologically relevant and serum-containing media. In vitro, PS-rLNPs showed no cytotoxicity across the tested dose range and exhibited pronounced intrinsic pro-healing bioactivity, significantly enhancing fibroblast viability and accelerating cell motility in both scratch-closure and Transwell migration assays. Collectively, these results establish PS-rLNPs as a biocompatible, serum-stable, and intrinsically pro-regenerative lipid nanoparticle system, providing a scalable route to tissue-derived nanomedicines that may complement next-generation wound-care strategies.