Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Eco-Friendly Microencapsulation of Lacticaseibacillus Paracasei Using Cissampelos pareira Leaf Extract as Natural Encapsulating Materials

Sasi Vimon
,
Chonticha Romyasamit
,
Rawiwan Chanpakdee
,
Sumaree Boonplu
,
Chackrit Nuengjamnong
,
Fonthip Makkliang
,
Suthinee Sangkanu
,
Veeranoot Nissapatorn
,
Phirabhat Saengsawang
,
Tina S. Dalgaard
,
Watcharapong Mitsuwan
* ORCID logo
Version 1 : Received: 4 March 2024 / Approved: 4 March 2024 / Online: 4 March 2024 (15:33:44 CET)

How to cite: Vimon, S.; Romyasamit, C.; Chanpakdee, R.; Boonplu, S.; Nuengjamnong, C.; Makkliang, F.; Sangkanu, S.; Nissapatorn, V.; Saengsawang, P.; Dalgaard, T.S.; Mitsuwan, W. Eco-Friendly Microencapsulation of Lacticaseibacillus Paracasei Using Cissampelos pareira Leaf Extract as Natural Encapsulating Materials. Preprints 2024, 2024030178. https://doi.org/10.20944/preprints202403.0178.v1 Vimon, S.; Romyasamit, C.; Chanpakdee, R.; Boonplu, S.; Nuengjamnong, C.; Makkliang, F.; Sangkanu, S.; Nissapatorn, V.; Saengsawang, P.; Dalgaard, T.S.; Mitsuwan, W. Eco-Friendly Microencapsulation of Lacticaseibacillus Paracasei Using Cissampelos pareira Leaf Extract as Natural Encapsulating Materials. Preprints 2024, 2024030178. https://doi.org/10.20944/preprints202403.0178.v1

Abstract

Microencapsulation using polymer materials is a potent process to protect and prolong the survival of probiotics. Cissampelos pareira leaf contains natural gelling agents that possess solidifying properties. This study aimed to investigate the development of microencapsulation containing Lacticaseibacillus paracasei using C. pareira extract as a natural encapsulating material. The absorption bands near 1603 cm−1 and 1725 cm−1 detected by Infrared spectroscopy (FTIR) were identified as pectin in C. pareira structure. The L. paracasei-C. pareira microcapsules (LP-CP) showed high encapsulation efficiency by 90.5% which was confirmed by the evaluation of their survival rate. Under thermal conditions (85oC), bacterial viability detected in the microcapsules was 69% as opposed to non-encapsulated bacteria where viability was as low as 5%. Furthermore, the microcapsule presented 75% bacterial viability whereas the free cells showed 30% under acidic conditions (pH 2). During storage conditions, LP-CP viability remained at 50% when the storage time was extended to 90 days whereas, the survival rate of free cells significantly decreased by 100% after 90 days. This information suggests that C. pareira is a potent polymer which can be used as an eco-friendly material for the microencapsulation of probiotics relevant as dietary supplementation for humans or animals.

Keywords

Cissampelos pareira; Lacticaseibacillus paracasei; microencapsulation; natural encapsulating material; probiotic supplementation

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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