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Genome-Wide Identification of the DGK Gene Family in Kiwifruit (Actinidia valvata Dunn) and Expression Analysis of Their Responses to Waterlogging Stress
Zhang, M.; Liu, C.; Wang, F.; Liu, S.; Gao, J.; Li, J.; Mo, Q.; Ye, K.; Qi, B.; Gong, H. Genome-Wide Identification of the DGK Gene Family in Kiwifruit (Actinidia valvata Dunn) and an Expression Analysis of Their Responses to Waterlogging Stress. Horticulturae2024, 10, 310.
Zhang, M.; Liu, C.; Wang, F.; Liu, S.; Gao, J.; Li, J.; Mo, Q.; Ye, K.; Qi, B.; Gong, H. Genome-Wide Identification of the DGK Gene Family in Kiwifruit (Actinidia valvata Dunn) and an Expression Analysis of Their Responses to Waterlogging Stress. Horticulturae 2024, 10, 310.
Zhang, M.; Liu, C.; Wang, F.; Liu, S.; Gao, J.; Li, J.; Mo, Q.; Ye, K.; Qi, B.; Gong, H. Genome-Wide Identification of the DGK Gene Family in Kiwifruit (Actinidia valvata Dunn) and an Expression Analysis of Their Responses to Waterlogging Stress. Horticulturae2024, 10, 310.
Zhang, M.; Liu, C.; Wang, F.; Liu, S.; Gao, J.; Li, J.; Mo, Q.; Ye, K.; Qi, B.; Gong, H. Genome-Wide Identification of the DGK Gene Family in Kiwifruit (Actinidia valvata Dunn) and an Expression Analysis of Their Responses to Waterlogging Stress. Horticulturae 2024, 10, 310.
Abstract
Diacylglycerol kinase (DGK) is a lipid kinase that phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). Based on converting one important signaling molecule (DAG) to another (PA), DGK plays an important role in plant responses to abiotic stress including waterlogging stress. However, no studies have been reported on the characterization of the DGK gene family in the waterlogging-tolerant kiwifruit germplasm, Actinidia valvata Dunn. Here, we identified 18 AvDGK genes in the A. valvata genome. The phylogenetic analysis showed that AvDGKs can be classified into three clusters, and members within the same cluster have similar domain distribution, exon-intron structures and conserved motif compositions. Chromosome localization analysis revealed that all the AvDGK genes are located across 18 different chromosomes. There were 29 duplicated gene pairs in kiwifruit and all had undergone purifying selection during evolution. Promoter cis-element analysis revealed that the cis-elements within AvDGK genes are associated with multiple functions, including phytohormone signal transduction, stress response, and plant growth and development. The expression pattern analyses indicated that AvDGK play an important role in the fruit development and plant response to waterlogging stress. AvDGKs gene family in the tetraploid A. valvata genome might promote PA synthesis and subsequent signal transduction both under short-term and long-term waterlogging stress. These results provide information regarding the structural characteristics and potential function of DGK genes within kiwifruit and lay a fundamental basis for further research into breeding for enhancing the kiwifruit’s tolerance to waterlogging stress.
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