Altmetrics
Downloads
318
Views
325
Comments
0
A peer-reviewed article of this preprint also exists.
This version is not peer-reviewed
Submitted:
06 February 2024
Posted:
06 February 2024
You are already at the latest version
Field of Application | Edited Gene(s) | Trait Category | Trait | Reference |
Abiotic stress tolerance | BnCUP1 (Cd uptake-related gene) | Cadmium tolerance | Reduced Cadmium (Cd) accumulation without a distinct compromise in yield, also for agricultural production in Cd-contaminated soils. | https://doi.org/10.3390/cells11233888 |
Abiotic stress tolerance | CUP1 (Cd uptake-related) | Cadmium tolerance | Reducing Cd accumulation. Displayed superior growth and longer roots. | https://doi.org/10.3390/cells11233888 |
Abiotic stress tolerance | BnPUB18 and BnPUB19 (Plant U-box) | Drought tolerance | Significant improvements to drought tolerance. | https://doi.org/10.1016/j.indcrop.2023.116875 |
Abiotic stress tolerance. Harvesting processing | BraRGL1 (DELLA protein) | Changed flowering time. Changed plant architecture | Early maturing varieties. Promotes the flower bud differentiation without affecting the stalk quality. Improved breeding of early maturing varieties (bolting and flowering). | https://doi.org/10.1093/hr/uhad119 |
Biotic stress tolerance | BnIDA (Inflorescence Deficient in Abscission) | Changed flowering time. Fungal resistance | Floral abscission-defective phenotype in which floral organs remained attached to developing siliques, and dry and colourless senesced floral parts remained attached to mature siliques. Enhanced resistance against Sclerotinia sclerotiorum (Sclerotinia stem rot (SSR)). Longer flowering period. | https://doi.org/10.1093/plphys/kiac364 |
Biotic stress tolerance | WRKY70 (WRKY transcription factors) | Fungal resistance | Enhanced resistance to Sclerotinia sclerotiorum (Sclerotinia stem rot (SSR)). | https://doi.org/10.3390/ijms19092716 |
Biotic stress tolerance | BnCRT1a (calreticulin) | Fungal resistance | Activation of the ethylene signalling pathway, which may contribute to reduced susceptibility towards Verticillium longisporum (Vl43). | https://doi.org/10.1111/pbi.13394 |
Biotic stress tolerance. Harvest properties. Storage properties | BnF5H (Ferulate-5-hydroxylase gene) | Fungal resistance. Changed plant architecture. | Decreased S/G lignin compositional ratio (ratio of syringyl (S) and guaiacyl (G) units in lignin). Stem strength dependence on lignin composition / stem lodging. More tightly packed stem structure, probably leading to a lower stem lodging index. Improves Sclerotinia sclerotiorum resistance. | https://doi.org/10.1111/pce.14208 |
Biotic stress tolerance. Yield | BnaIDA (inflorescence deficient in abscission) | Fungal resistance. Changed flowering time. | Reduced floral organ abscission, silique dehiscence (diverge), and disease severity caused by S. sclerotiorum. Improved yield by reducing seed loss due to premature silique dehiscence during mechanical harvesting and losses due to stem rot. Longer flowering period. | https://doi.org/10.1016/j.xplc.2022.100452 |
Breeding processing | BnS6-Smi2 (S locus) | Avoiding self-fertilization | Self-incompatibility to prevent inbreeding in hermaphrodite angiosperms via the rejection of self-pollen. | https://doi.org/10.1111%2Fpbi.13577 |
Breeding processing | BnaDMP (domain of unknown function 679 membrane protein) | Doubled haploid induction | Establishment of maternal haploid induction. | https://doi.org/10.1111/jipb.13244 |
Breeding properties | BnaDMP (domain of unknown function 679 membrane protein) | Doubled haploid induction | Higher haploid induction rate. | https://doi.org/10.1111/jipb.13270 |
Breeding properties | BnCYP704B1 (cytochrome P450) | Male sterility | Establishment of male sterility: pollenless, sterile phenotype in mature anthers. | https://doi.org/10.3390/plants12020365 |
Breeding properties | BnARC1 (E3 ligaseARM-Repeat-Containing protein) | Enables self-fertilization | Complete breakdown of self-incompatibility response. Promoting outcrossing and genetic diversity. | https://doi.org/10.1016/j.xplc.2022.100504 |
Food quality | BnaSAD2 | Changed fatty acid content | Higher stearic acid content. | https://doi.org/10.1007/s00122-023-04414-x |
Food quality. Feed quality | BnITPK (inositol tetrakisphosphate kinase) | Changed protein value | Reduced phytic acid, increase of free phosphorus, increase in protein value and no adverse effects on oil contents. | https://doi.org/10.1111/pbi.13380 |
Food quality. Feed quality. Industrial properties | BnFAD2 (fatty acid desaturase 2) | Changed fatty acid composition | Increased oleic acid content. | https://doi.org/10.1016/j.plaphy.2018.04.025 |
Food quality. Feed quality. Industrial properties | BnTT8 (basic helix-loop-helix, bHLH) | Changed fatty acid composition | Modification of fatty acid composition, including increases in palmitic acid, linoleic acid and linolenic acid and decreases in stearic acid and oleic acid. | https://doi.org/10.1111/pbi.13281 |
Food quality. Feed quality. Industrial properties | BnFAD2 (fatty acid dehydrogenase 2) | Changed fatty acid composition | Modification of fatty acid composition. The oleic acid content in the seed increased significantly, while linoleic and linolenic acid contents decreased accordingly. | https://doi.org/10.1007/s00122-020-03607-y |
Food quality. Feed quality. Industrial properties | BnFAD2 (fatty acid desaturase 2) and BnFAE1 (fatty acid elongase1) | Changed fatty acid composition | Increased content of oleic acid, reduced erucic acid levels and slightly decreased polyunsaturated fatty acids content. | https://doi.org/10.3390/genes13101681 |
Food quality. Feed quality. Industrial properties | KASII (canolaβ-ketoacyl-ACP synthase II) | Changed fatty acid composition. Changed oil content | Decreased palmitic acid content, increased total C18 and reduced total saturated fatty acid contents. | https://doi.org/10.1111/j.1467-7652.2012.00695.x |
Food quality. Feed quality. Industrial properties | BnaTT7, BnaTT18, BnaTT10, BnaTT1, BnaTT2 or BnaTT12 (transparent testa) | Changed fatty acid composition. Changed oil content | Elevated seed oil content and decreased pigment and lignin accumulation. Decreased oleic acid and increased linoleic and linolenic acid contents. Down-regulation of key genes in flavonoid synthesis. | https://doi.org/10.1111/pbi.14197 |
Food quality. Feed quality. Industrial properties | BnTT2 (transparent testa 2) | Changed fatty acid composition. Changed oil content | Reduced flavonoids and improved fatty acid composition with higher linoleic acid and linolenic acid. | https://dx.doi.org/10.1021/acs.jafc.0c01126 |
Food quality. Feed quality. Industrial properties | BnaFAE1 (fatty acid elongase 1) | Changed fatty acid composition. Changed oil content | Deacreased erucic acid content. | https://doi.org/10.3389/fpls.2022.848723 |
Food quality. Feed quality. Industrial properties | BnCIPK9 (Calcineurin B-like (CBL)-interacting protein kinase 9) | Changed fatty acid composition. Changed oil content | Regulate seed oil metabolism. Increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids. | https://doi.org/10.1093/plphys/kiac569 |
Food quality. Feed quality. Industrial properties | BnSFAR4 and BnSFAR5 (seed fatty acid reducer) | Changed oil content | Increased seed oil content without pleiotropic effects on seed germination, vigour and oil mobilization. Improving oil yield. | https://doi.org/10.1111/pbi.13381 |
Food quality. Feed quality. Industrial properties | BnLPAT2 and BnLPAT5 (Lysophosphatidic acid acyltransferase) | Changed oil content | Increased seed oil content. | https://doi.org/10.1186/s13068-022-02182-2 |
Food quality. Feed quality. Industrial properties | BnFAD2 (fatty acid desaturase 2) | Changed fatty acid composition. Changed oil content | Enhanced seed oleic acid content. | https://doi.org/10.3389/fpls.2022.1034215 |
Food quality. Feed quality. Industrial properties | BnKANT3, BnGIF1, BnAGP11 or BnEDA32 | Changed fatty acid composition. Changed oil content | Increased linoleic or linolenic acid content. | https://doi.org/content/33/5/798.full |
Food quality. Feed quality. Industrial properties | BnaSBE (starch branching enzymes) | Changed plant architecture. Changed carbohydrate composition. | Higher starch-bound phosphate content and altered pattern of amylopectin length pattern. Thick main stem. | https://doi.org/10.1093/plphys/kiab535 |
Harvest properties | BnaCOL9 (CONSTANS-like 9) | Changed flowering time | Early-maturing breeding. | https://doi.org/10.3390/ijms232314944 |
Harvest properties | BnBRI1 (leucine-rich repeat receptor-like protein kinase) | Changed plant architecture | Semi-dwarf lines without decreased yield in order to increase harvest index. | https://doi.org/10.3389/fpls.2022.865132 |
Harvest properties. | BnJAG (jagged) | Changed plant architecture | Changes in pod dehiscence zone with potential to increase shatter resistance. | https://doi.org/10.3390/biom9110725 |
Harvest properties. | BnIND (INDEHISCENT) | Changed plant architecture | Increased shatter resistance to avoid seed loss during mechanical harvest. | https://doi.org/10.1007/s00122-019-03341-0 |
Harvest properties. Yield | BnALC (ALCATRAZ) | Changed plant architecture | Increased shatter resistance to avoid seed loss during mechanical harvest. | https://doi.org/10.1104/pp.17.00426 |
Harvesting processing | BnaSVP (Short Vegetative Phase) | Changed flowering time | Early-flowering phenotypes. | https://doi.org/10.1016/j.cj.2021.03.023 |
Seed quality | BnPAP2 (production of anthocyaninpigment 2) | Changed seed pigments | Yellow seed coat and reduced proanthocyanidins. Reduced expression of various flavonoid biosynthesis genes. | https://doi.org/10.1016/j.jia.2023.05.001 |
Visual appearance | BnaCRTISO (carotenoid isomerase) | Changed ornamental plant properties | Altered colour of petals and leaves in order to improve the ornamental value of rapeseed and promote the development of agriculture and tourism. | https://doi.org/10.3389/fpls.2022.801456 |
Yield | BnaSDG8 (Methyltransferase SDG8) | Changed flowering time | Early-flowering varieties influenced by epigenetic modification. | https://doi.org/10.1111/tpj.13978 |
Yield | BnCLV3 (CLAVATA3) | Changed plant architecture | Increased silique and seed number and higher seed weight. | https://doi.org/10.1111/pbi.12872 |
Yield | BnaMAX1 (more axillary growth (max)) | Changed plant architecture | Increased branching phenotypes with more siliques in order to increased yield. | https://doi.org/10.1111/pbi.13228 |
Yield | BnD14 (strigolactone receptor BnD14) | Changed plant architecture | Shoot architectural changes. Increase of total flowers. | https://doi.org/10.1111/pbi.13513 |
Yield | BnaA03.BP (BREVIPEDICELLUS) | Changed plant architecture | Optimizing rapeseed plant architecture, semi-dwarf and compact architecture. | https://doi.org/10.1111/pbi.13703 |
Yield | BnaEOD3 (ENHANCER OF DA1) | Changed plant architecture | Shorter siliques, smaler seeds, and an increased number of seeds per siliques. Increased seed weight per plant. | https://doi.org/10.1002/jcp.29986 |
Yield | BnEOD1 (Enhancer of DA1) | Changed plant architecture | Increased seed size and weight. | https://doi.org/10.21203/rs.3.rs-3204656/v1 |
Field of Application | Edited Gene(s) | Trait Category | Trait | Reference |
Food quality. Industrial properties | CsFAD2 (fatty acid desaturase 2) | Changed fatty acid composition. Changed oil content | Increased oleic acid content (proportional decrease in linoleic and linolenic acid content). | https://doi.org/10.1111/pbi.12671 |
Food quality. Industrial properties | CsFAD2 (fatty acid desaturase 2) | Changed fatty acid composition | Increased oleic acid content (proportional decrease in linoleic and linolenic acid content). | https://doi.org/10.1111/pbi.12663 |
Industrial properties | CsFAD2 (fatty acid desaturase 2) | Changed fatty acid composition | Enhanced monounsaturated fatty acid levels, partially bushy phenotype. | https://doi.org/10.3389/fpls.2021.702930 |
Food quality. Feed quality.Industrial properties | CsCRUC (cruciferin C) | Changed protein composition. Changed fatty acid composition | Changed seed amino acid content (increased proportion of alanine, cysteine and proline, and decrease of isoleucine, tyrosine and valine). Increased relative abundance of all saturated fatty acids. | https://doi.org/10.1186/s12870-019-1873-0 |
Industrial properties | CsDGAT1 or CsPDAT1 (acyl-CoA:- or phospholipid:diacylglycerol acyltransferase) | Changed triacylglycerols content. Changed fatty acid composition. Changed oil content | Produce triacylglycerols (TAGs) that are valuable as industrial feedstocks. Reduced oil content, partially higher levels of linoleic acid. | https://doi.org/10.1093/pcp/pcx058 |
Food quality | FAE1 (fatty acid elongase 1) | Changed fatty acid composition | Decreased erucic acid content, increased levels of omega-3 fatty acids such as linolenic acid as well as eicosapentaenoic and docosahexaenoic acid in transgenic camelina. | https://doi.org/10.1111/pbi.13876 |
Food quality | FAE1 (fatty acid elongase 1) | Changed fatty acid composition | Increased oleic and linolenic acid content by blocking eicosenoic and erucic acid synthesis in transgenic camelina. | https://doi.org/10.1038/s41598-023-34364-9 |
Food quality | FAE1 (fatty acid elongase 1) | Changed fatty acid composition | Reduction of C20-C24 very long-chain fatty acids (VLCFAs). | https://doi.org/10.1016/j.plaphy.2017.11.021 |
Yield | FLC (flowering locus C), SVP (short vegetative phase), LHP1 (like heterochromatin protein 1), TFL1 (terminal flower 1) and EFL3 (early flowering locus 3) | Changed flowering time | Early-flowering, shorter stature and/or basal branching. Different combinations of mutations had a positive or negative impact on yield. | https://doi.org/10.3390/agronomy12081873 |
Food quality. Feed quality | CsGTR1 and CsGTR2 (glucosinolate transporter) | Changed glucosinolate content | Decreased and eliminated glucosinolate content in order to improve quality of oil and press cake. | https://doi.org/10.1111/pbi.13936 |
Field of Application | Edited Gene | Trait Category | Trait | Reference |
Food quality. Feed quality. Industrial properties | FAD2 (fatty acid desaturase 2), ROD1 (reduced oleate desaturation 1) and FAE1 (fatty acid elongation 1) | Changed fatty acid composition. Changed oil content. | Increased oleic acid amount in seed oil. Reduction of PUFAs. | https://doi.org/10.3389/fpls.2021.652319 |
Food quality | FAE1 (fatty acid elongation 1) | Changed fatty acid composition. | Abolishing erucic acid production and creating an edible seed oil comparable to that of canola. | https://doi.org/10.1111/pbi.13014 |
Industrial properties | FAE1-3 (fatty acid elongation) | Changed fatty acid composition. | Abolishing erucic acid production, further crossing with transgenic pennycress. | https://doi.org/10.3389/fenrg.2021.620118 |
Year of Publication | Notification Number | Company/Institute | Title | Plant | Country | Period of Release |
2023 | 23/Q02 | Rothamsted Research | Field assessment of gene edited Oilseed Rape with pod shatter resistance | Brassica napus | UK | 2023-2028 |
2023 | 23/R08/01 | Rothamsted Research | Synthesis and accumulation of seed storage compounds in Camelina sativa | Camelina sativa | UK | 2023-2026 |
2023 | B/SE/23/4198 | Umeå University | Arabidopsis - photosynthesis and hormone biology | Arabidopsis thaliana | Sweden | 2023-2027 |
2019 | B/GB/19/R08/01 | Rothamsted Research | Synthesis and accumulation of seed storage compounds in Camelina sativa | Camelina sativa | United Kingdom | 2019-2023 |
2019 | B/GB/19/52/01 | John Innes Centre | Genetic regulation of Sulphur metabolism in Brassica oleracea | Brassica oleracea (broccoli) | United Kingdom | 2019-2021 |
Year of Application | Plant | Applicant | Method | Trait* |
2022 | Brassica juncea | Pairwise Plant Services, Inc | CRISPR/Cas9 | Reduced pungency and reduced trichome production |
2022 | Pennycress | Hjelle Advisors (CoverCress) | CRISPR/Cas9 | Lowered erucic acid and lowered fiber in seeds |
2020 | Canola | Corteva Agriscience | CRISPR/Cas9 | Altered Meal Qualities |
2020 | Pennycress | CoverCress | CRISPR/Cas9 (?) | CBI |
2020 | Pennycress | Illinois State University | CRISPR/Cas9 (?) | Development of pennycress as an oilseed-producing cover crop |
2020 | Canola | Yield10 Bioscience | CRISPR/Cas9 | Altered Oil Content |
2020 | Pennycress | CoverCress Inc. | CRISPR/Cas9 | CBI |
2020 | Camelina | Yield10 Bioscience | CRISPR/Cas9 | CBI |
2020 | Pennycress | CoverCress Inc | CRISPR/Cas9 | CBI |
2019 | Pennycress | Illinois State University | CRISPR/Cas9 | CBI |
2018 | Camelina | Yield10 Bioscience | CRISPR/Cas9 | CBI |
2018 | Pennycress | Illionois State University | CRISPR/Cas9 | Altered Oil Content |
2018 | Camelina | Yield 10 | CRISPR/Cas9 | Altered Oil Content |
2017 | Camelina | Yield 10 | CRISPR/Cas9 | Altered Oil Content |
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2024 MDPI (Basel, Switzerland) unless otherwise stated