Yang, C.; Wang, L.-Y.; Li, Y.-K.; Lin, J.-T.; Chen, D.-K.; Yao, N. Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome. Plants2024, 13, 299.
Yang, C.; Wang, L.-Y.; Li, Y.-K.; Lin, J.-T.; Chen, D.-K.; Yao, N. Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome. Plants 2024, 13, 299.
Yang, C.; Wang, L.-Y.; Li, Y.-K.; Lin, J.-T.; Chen, D.-K.; Yao, N. Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome. Plants2024, 13, 299.
Yang, C.; Wang, L.-Y.; Li, Y.-K.; Lin, J.-T.; Chen, D.-K.; Yao, N. Arabidopsis Leaf Chloroplasts Have a Specific Sphingolipidome. Plants 2024, 13, 299.
Abstract
Sphingolipids are ubiquitous in eukaryotes and certain prokaryotes, where they serve as vital components of biological membranes and as bioactive molecules. Chloroplasts have complex membrane structures that play crucial roles in photosynthesis, but their specific sphingolipidome remains unreported. In this study, we used high-performance liquid chromatography-mass spectrometry (HPLC-MS/MS) to analyze the sphingolipidome of purified Arabidopsis thaliana chloroplasts. We detected 92 chloroplast sphingolipids. The chloroplast sphingolipidome differed from total leaf (TL) samples, having a higher content of free long-chain bases and hydroxyceramides, and a greater proportion of complex sphingolipids with 16C fatty acid (FA) forms. Notably, chloroplast glucosylceramides were predominantly the d18:1 h16:0 and t18:1 h16:0 forms, rather than the 24C FA form found in TL and other cellular structures. Comparing the sphingolipidomes of different cellular structures underscores the inhomogeneity of intracellular distribution of sphingolipids. This provides a robust reference for further elucidating the function of sphingolipids in plant cells.
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