PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test-Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil
Mahdavi, R.; Martinkovic, F.; Shams-Eldin, H.; Pereira, I.E.; Reis, A.B.; Latz, A.; Heinz, D.; Aira, C.; Fresco-Taboada, A.; Abass, E.; Romero-Olmedo, J.; Teixeira, H.C.; Steinhoff, U. Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil. Pathogens2024, 13, 109.
Mahdavi, R.; Martinkovic, F.; Shams-Eldin, H.; Pereira, I.E.; Reis, A.B.; Latz, A.; Heinz, D.; Aira, C.; Fresco-Taboada, A.; Abass, E.; Romero-Olmedo, J.; Teixeira, H.C.; Steinhoff, U. Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil. Pathogens 2024, 13, 109.
Mahdavi, R.; Martinkovic, F.; Shams-Eldin, H.; Pereira, I.E.; Reis, A.B.; Latz, A.; Heinz, D.; Aira, C.; Fresco-Taboada, A.; Abass, E.; Romero-Olmedo, J.; Teixeira, H.C.; Steinhoff, U. Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil. Pathogens2024, 13, 109.
Mahdavi, R.; Martinkovic, F.; Shams-Eldin, H.; Pereira, I.E.; Reis, A.B.; Latz, A.; Heinz, D.; Aira, C.; Fresco-Taboada, A.; Abass, E.; Romero-Olmedo, J.; Teixeira, H.C.; Steinhoff, U. Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil. Pathogens 2024, 13, 109.
Abstract
Control of canine infections with Leishmania infantum (L. infantum), a major zoonotic disease in Brazil and southern Europe is becoming increasingly important due to their close proximity to humans, the increasing import of dogs from endemic regions and impact of climate change on vector spreading. Simple, rapid and reliable diagnostic tests are therefore needed to detect infected dogs.
Here, we re-evaluated different serological methods for the diagnosis of canine leishmaniosis (CanL) in Croatia and Brazil. The diagnostic performance of the indirect fluorescent antibody test (IFAT) and the VetLine® Leishmania ELISA was compared with three rKLi8.3 based diagnostic test systems, the rKLi8.3 ELISA, the INgezim® Leishma CROM lateral flow test (LFT) and the VetBlot® Leishmania LineBlot.
CanL symptomatic dogs were efficiently diagnosed by all tests, except the VetLine® Leishmania ELISA, which is based on whole Leishmania antigens. The advantage of rKLi8.3 was also observed in oligo-and asymptomatic dogs from Brazil and Croatia, although with reduced diagnostic efficiency compared to symptomatic dogs. Similar to IFAT and rKLi8.3 ELISA, the LFT did not cross-react with other common canine pathogens, showed a very high specificity for healthy dogs from endemic regions of both countries and did not react with healthy, vaccinated dogs in Brazil.
In conclusion, sero-diagnostic tests based on the rKLi8.3 antigens are superior to whole parasite antigens and the LFT has the advantage of providing a laboratory independent, rapid and specific diagnosis of CanL.
Keywords
Canine leishmaniosis; CanL; POC diagnostics for leishmaniosis; lateral flow test; line blot; Leishmania improved sero-diagnostics; Leishmania ELISA
Subject
Biology and Life Sciences, Parasitology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
