Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Agro-Morphological Traits and Molecular Diversity of Proso Millet (Panicum miliaceum L.) Affected by the Various Colchicine Treatments

Aiym Zeinullina
ORCID logo ,
Meisam Zargar
* ORCID logo ,
Elmira Dyussibayeva
,
Aidyn Orazov
,
Irina Zhirnova
,
Gulzat Yessenbekova
,
Lyudmila Zotova
,
Aiman Rysbekova
* ORCID logo ,
Yin-Gang Hu
Version 1 : Received: 2 November 2023 / Approved: 3 November 2023 / Online: 3 November 2023 (10:59:14 CET)

A peer-reviewed article of this Preprint also exists.

Zeinullina, A.; Zargar, M.; Dyussibayeva, E.; Orazov, A.; Zhirnova, I.; Yessenbekova, G.; Zotova, L.; Rysbekova, A.; Hu, Y.-G. Agro-Morphological Traits and Molecular Diversity of Proso Millet (Panicum miliaceum L.) Affected by Various Colchicine Treatments. Agronomy 2023, 13, 2973. Zeinullina, A.; Zargar, M.; Dyussibayeva, E.; Orazov, A.; Zhirnova, I.; Yessenbekova, G.; Zotova, L.; Rysbekova, A.; Hu, Y.-G. Agro-Morphological Traits and Molecular Diversity of Proso Millet (Panicum miliaceum L.) Affected by Various Colchicine Treatments. Agronomy 2023, 13, 2973.

Abstract

. Colchicine (C22H25NO6) is a substance used for inducing mutations in order to regulate important agronomic traits. The objective of this study was to investigate the effect of different concentrations (0.0, 0.04, 0.06, 0.08 and 0.1%) and treatment time (6, 12 and 24 h) of colchicine on agronomic traits of proso millet (Panicum miliaceum L.) and to assess the genetic diversity of M2 generations using inter simple sequence repeat markers (ISSR). The experiment was conducted in 2021 for the M1-generation and in 2022 for the M2-generation, from May to September. The percentage of the field germination was decreased with the increasing colchicine concentration and exposure duration. The chlorophyll-defective M1-M2 plants were obtained using various concentrations and exposure periods of colchicine. The highest amount of mutational modifications was attained at 0.08-0.1% concentrations colchicine. A total of 248 plant families with chlorophyll-defective mutations based on the research results were selected from 2214 families. The growing season of M1- and M2 plants were diminished under higher colchicine concentration (0.08-0.1%) combined with soaking time. Thus, the highest indicator of growing season (84 days) was observed at six h treatment time for PI 289324, while the fewest (78 days) was recorded at 12 and 24 h. The possibility of obtaining morphological mutations using colchicine has been confirmed. The ISSR primers amplified a total of 1333 fragments, 1281 bands were found to be polymorphic and 52 bands monomorphic. The percentage of polymorphism varied from 80 to 100 % with an average of 96.11%. Our results showed that most of the bands were found at 0.08% colchicine concentration. These positive variations are a great opportunity to use colchicine as a tool for improving agronomic traits in plant breeding.

Keywords

proso millet; mutagenesis; colchicine; chlorophyll deficient; agronomic traits; ISSR markers

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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