Analysis of Rat Brainstem Transcriptome Changes after Acute Stress

Dmitriy A. Lanshakov; Ekaterina V Sukhareva; Veta V Bulygina; Anna A. Khozyainova; Tatiana S. Gerashchenko; Evgeny V. Denisov; Tatiana S Kalinina

doi:10.20944/preprints202310.1756.v1

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preprints.org > biology and life sciences > neuroscience and neurology > doi: 10.20944/preprints202310.1756.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Analysis of Rat Brainstem Transcriptome Changes after Acute Stress

Dmitriy A. Lanshakov

^* ,

Ekaterina V Sukhareva

Veta V Bulygina

Anna A. Khozyainova

Tatiana S. Gerashchenko

Evgeny V. Denisov

Tatiana S Kalinina

Version 1 : Received: 26 October 2023 / Approved: 27 October 2023 / Online: 27 October 2023 (05:24:47 CEST)

How to cite: Lanshakov, D.A.; Sukhareva, E.V.; Bulygina, V.V.; Khozyainova, A.A.; Gerashchenko, T.S.; Denisov, E.V.; Kalinina, T.S. Analysis of Rat Brainstem Transcriptome Changes after Acute Stress. Preprints 2023, 2023101756. https://doi.org/10.20944/preprints202310.1756.v1 Lanshakov, D.A.; Sukhareva, E.V.; Bulygina, V.V.; Khozyainova, A.A.; Gerashchenko, T.S.; Denisov, E.V.; Kalinina, T.S. Analysis of Rat Brainstem Transcriptome Changes after Acute Stress. Preprints 2023, 2023101756. https://doi.org/10.20944/preprints202310.1756.v1

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MDPI and ACS Style

Lanshakov, D.A.; Sukhareva, E.V.; Bulygina, V.V.; Khozyainova, A.A.; Gerashchenko, T.S.; Denisov, E.V.; Kalinina, T.S. Analysis of Rat Brainstem Transcriptome Changes after Acute Stress. Preprints 2023, 2023101756. https://doi.org/10.20944/preprints202310.1756.v1

APA Style

Lanshakov, D.A., Sukhareva, E.V., Bulygina, V.V., Khozyainova, A.A., Gerashchenko, T.S., Denisov, E.V., & Kalinina, T.S. (2023). Analysis of Rat Brainstem Transcriptome Changes after Acute Stress. Preprints. https://doi.org/10.20944/preprints202310.1756.v1

Chicago/Turabian Style

Lanshakov, D.A., Evgeny V. Denisov and Tatiana S Kalinina. 2023 "Analysis of Rat Brainstem Transcriptome Changes after Acute Stress" Preprints. https://doi.org/10.20944/preprints202310.1756.v1

Abstract

In this work, we tried to emphasize whole transcriptome expression changes in the Wistar rats brainstem two hours after acute stress. Most of the genes were upregulated. We detected massive shift of neuropeptides Crh, Trh,Cga, Tshb, Uts2b, Tac4, Lep and neuropeptide receptors Hcrtr1, Sstr5, Bdkrb2, Crhr2 signaling, as well as Glutamate Grin3b, Grm2 and GABA Gpr156, Acetylcholine Chrm4,Chrne, Adreno- Adra2b receptors expression uprising. Intensive increase in expression of intermediate filaments Krt83/Krt86/Krt80/Krt84/Krt87/Krt4/Krt76 and motor proteins Myo7a, Klc3 was detected. Remarkably, at this time we also observed signs of microglia activation. Both expression of anti-inflammatory cytokines Il13, Ccl24 and proinflammatory cytokin’s receptors Il9r, Il12rb1, Tnfrsf14, Tnfrsf13c, Tnfrsf25, Tnfrsf1b were increased. In the Wnt signaling pathway, we have seen increased expression of ligands-receptors Wnt1, Wnt11, Ror2, and also negative regulators Notum, Sfrp5, Sost. Also we conducted cDNA libraries normalization with duplex specific nuclease and compared results. On the basis of our RNAseq data, we chose reference genes for RT-qPCR best suitable for experiments with acute stress.

Keywords

RNAseq; DSN; FST; acute stress; brainstem; Transcriptome

Subject

Biology and Life Sciences, Neuroscience and Neurology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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