Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transcriptome Analysis Revealed Potential Genes of Skeletal Muscle Thermogenesis in Mashen Pigs and Large White Pigs under Cold Stress

Wenxia Li
ORCID logo ,
Yufen Chen
,
Yunting Zhang
,
Ning Zhao
,
Wanfeng Zhang
,
Mingyue Shi
,
Yan Zhao
,
Chunbo Cai
,
Chang Lu
,
Pengfei Gao
ORCID logo ,
Xiaohong Guo
,
Bugao Li
ORCID logo ,
Sung Woo Kim
ORCID logo ,
Yang Yang
* ,
Guoqing Cao
*
Version 1 : Received: 9 September 2023 / Approved: 11 September 2023 / Online: 12 September 2023 (04:55:16 CEST)

A peer-reviewed article of this Preprint also exists.

Li, W.; Chen, Y.; Zhang, Y.; Zhao, N.; Zhang, W.; Shi, M.; Zhao, Y.; Cai, C.; Lu, C.; Gao, P.; Guo, X.; Li, B.; Kim, S.-W.; Yang, Y.; Cao, G. Transcriptome Analysis Revealed Potential Genes of Skeletal Muscle Thermogenesis in Mashen Pigs and Large White Pigs under Cold Stress. Int. J. Mol. Sci. 2023, 24, 15534. Li, W.; Chen, Y.; Zhang, Y.; Zhao, N.; Zhang, W.; Shi, M.; Zhao, Y.; Cai, C.; Lu, C.; Gao, P.; Guo, X.; Li, B.; Kim, S.-W.; Yang, Y.; Cao, G. Transcriptome Analysis Revealed Potential Genes of Skeletal Muscle Thermogenesis in Mashen Pigs and Large White Pigs under Cold Stress. Int. J. Mol. Sci. 2023, 24, 15534.

Abstract

Pigs are susceptible to cold stress due to the absence of brown fat caused by the partial deletion of uncoupling protein 1 during their evolution. Some local pig breeds in China, exhibit potential cold adaptability, but research has primarily focused on fat and intestinal tissues. Skeletal muscle plays a key role in adaptive thermogenesis in mammals, yet the molecular mechanism of cold adaptation in porcine skeletal muscle remains poorly understood. This study investigated the cold adaptability of two pig breeds, Mashen pigs (MS) and Large White pigs (LW), in a 4-day cold (4°C) or room temperature (25°C) environment. We recorded phenotypic changes and collected blood, and longissimus dorsi muscle for transcriptome sequencing. Finally, a candidate gene was randomly selected for functional exploration in porcine skeletal muscle satellite cells. A decrease in body temperature and body weight in both LW and MS pigs under cold stress, accompanied by increased shivering frequency and respiratory frequency. However, MS pigs demonstrated stable physiological homeostasis, indicating a certain level of cold adaptability. LW pigs primarily responded to cold stress by regulating heat production and Glycolipid energy metabolism. MS pigs exhibited a distinct response to cold stress, involving regulation of heat production, energy metabolism pathways, robust fatty acid oxidation ability, and stronger immune response. Furthermore, the functional exploration of PRSS8 in porcine skeletal muscle satellite cells revealed it affected cellular energy metabolism and thermogenesis by regulating ERK phosphorylation. These findings shed light on the diverse transcriptional responses of skeletal muscle in LW and MS pigs under cold stress, offering valuable insights into the molecular mechanisms underlying cold adaptation in pigs.

Keywords

cold stress; skeletal muscle; transcriptome; Large White pig; Mashen pig; PRSS8

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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