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Dual RNA-Seq Analysis Pinpoints to a BalancedRegulation between Symbiosis and Immunity in the Medicago truncatula-Sinorhizobium meliloti Symbiotic Interactions
Zhang, D.; Wu, Q.; Zhao, Y.; Yan, Z.; Xiao, A.; Yu, H.; Cao, Y. Dual RNA-Seq Analysis Pinpoints a Balanced Regulation between Symbiosis and Immunity in Medicago truncatula-Sinorhizobium meliloti Symbiotic Nodules. Int. J. Mol. Sci.2023, 24, 16178.
Zhang, D.; Wu, Q.; Zhao, Y.; Yan, Z.; Xiao, A.; Yu, H.; Cao, Y. Dual RNA-Seq Analysis Pinpoints a Balanced Regulation between Symbiosis and Immunity in Medicago truncatula-Sinorhizobium meliloti Symbiotic Nodules. Int. J. Mol. Sci. 2023, 24, 16178.
Zhang, D.; Wu, Q.; Zhao, Y.; Yan, Z.; Xiao, A.; Yu, H.; Cao, Y. Dual RNA-Seq Analysis Pinpoints a Balanced Regulation between Symbiosis and Immunity in Medicago truncatula-Sinorhizobium meliloti Symbiotic Nodules. Int. J. Mol. Sci.2023, 24, 16178.
Zhang, D.; Wu, Q.; Zhao, Y.; Yan, Z.; Xiao, A.; Yu, H.; Cao, Y. Dual RNA-Seq Analysis Pinpoints a Balanced Regulation between Symbiosis and Immunity in Medicago truncatula-Sinorhizobium meliloti Symbiotic Nodules. Int. J. Mol. Sci. 2023, 24, 16178.
Abstract
Legume-rhizobial symbiosis leads to to the formation of root nodules, where rhizobia reside and develop into bacteroids to reduce nitrogen into ammonium for plant growth, which leaves an opening question as how plant immunity is attenuated in nodules in the presence of large number of foreign bacteria. In Medicago truncatula, mutation in NAD1 (Nodules with Activated Defense 1) only produces necrotic nodules with overactivated immunity, indicating NAD1 is an indispensable component required for suppressing immunity in nodules. In this study, a dual RNA-seq transcriptomic technology was performed to extensively analyze gene expressions in nad1-1 mutant nodules. We indenifited 89 DEGs in symbiotic nitrogen fixation and 89 DEGs in immunity in Medicago truncatula at 10 dpi. Simultaneously, we indenifited 27 DEGs in fix and nif genes in Sinorhizobium meliloti. Then we identified 56 DEGs related to stress, including ROS, NO, and NCR, most of which were upregulated in Sinorhizobium meliloti. Our analyses of nitrogen fixation-defective plant nad1-1 mutants with overactivated defense indicate that host use plant immunity to control massive bacterial colonization during early and late symbiotic stages. Our findings provide insight into the function of NAD1 in improvement or inhibition of plant immune response to maintain symbiosis during rhizobial endosymbiosis.
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