Hayashida, K.; Ogino, R.; Suda, S.; Yamakawa, S. Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model: A Preliminary Study. Lymphatics2023, 1, 237-243.
Hayashida, K.; Ogino, R.; Suda, S.; Yamakawa, S. Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model: A Preliminary Study. Lymphatics 2023, 1, 237-243.
Hayashida, K.; Ogino, R.; Suda, S.; Yamakawa, S. Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model: A Preliminary Study. Lymphatics2023, 1, 237-243.
Hayashida, K.; Ogino, R.; Suda, S.; Yamakawa, S. Scanning Electron Microscopy Analysis of Lymphatic Regeneration in a Secondary Lymphedema Mouse Model: A Preliminary Study. Lymphatics 2023, 1, 237-243.
Abstract
Under inflammatory conditions including lymphatic disorders, bone marrow-derived myeloid cells often express lymphatic endothelial cell (LEC) markers called LEC progenitor cells, which extend lymphatic vessels by fusing with existing lymphatic vessels. However, studies on the mechanism of lymphatic regeneration using three-dimensional images of lymphatic structures are limited. In this study, scanning electron microscopy (SEM) was used to observe the three-dimensional structure of lymphangiogenesis in a mouse model of secondary lymphedema. The model was established in C57BL/6J mice via circumferential incision in the inguinal region of the left hind limb. Skin samples were obtained from the lymphedema region on days 2, 5, and 8 after surgery. To determine lymphatic vessel positions using SEM analysis, we detected anti-lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) immunoreactivity in serial sections and overlaid them during SEM observation. On days 2 and 5, spherical cells, probably myeloid cells, were attached and fused to the LYVE-1-positive lymphatic vessel walls. On day 8, spherical cells were converted to string-shaped cells, forming a new lymphatic vessel wall resembling an intraluminal pillar. Our results show the three-dimensional lymphatic structures formed during lymphatic regeneration.
Keywords
lymphatic vessel; scanning electron microscopy; irradiated tissue; intussusceptive lymphangiogenesis; lymphedema
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
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