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Comparative Analysis of Plant Morphological Features and Molecular Genetic Data for Polymorphism Study in Genus Tulipa L. and Determining the Tulip Species
Version 1
: Received: 30 May 2023 / Approved: 1 June 2023 / Online: 1 June 2023 (03:06:34 CEST)
How to cite:
Semenova, M.V.; Olekhnovich, L.S.; Enina, O.L. Comparative Analysis of Plant Morphological Features and Molecular Genetic Data for Polymorphism Study in Genus Tulipa L. and Determining the Tulip Species. Preprints2023, 2023060008. https://doi.org/10.20944/preprints202306.0008.v1
Semenova, M.V.; Olekhnovich, L.S.; Enina, O.L. Comparative Analysis of Plant Morphological Features and Molecular Genetic Data for Polymorphism Study in Genus Tulipa L. and Determining the Tulip Species. Preprints 2023, 2023060008. https://doi.org/10.20944/preprints202306.0008.v1
Semenova, M.V.; Olekhnovich, L.S.; Enina, O.L. Comparative Analysis of Plant Morphological Features and Molecular Genetic Data for Polymorphism Study in Genus Tulipa L. and Determining the Tulip Species. Preprints2023, 2023060008. https://doi.org/10.20944/preprints202306.0008.v1
APA Style
Semenova, M.V., Olekhnovich, L.S., & Enina, O.L. (2023). Comparative Analysis of Plant Morphological Features and Molecular Genetic Data for Polymorphism Study in Genus<em> Tulipa</em> L. and Determining the <em>Tulip</em> Species. Preprints. https://doi.org/10.20944/preprints202306.0008.v1
Chicago/Turabian Style
Semenova, M.V., Ludmila S. Olekhnovich and Olga L. Enina. 2023 "Comparative Analysis of Plant Morphological Features and Molecular Genetic Data for Polymorphism Study in Genus<em> Tulipa</em> L. and Determining the <em>Tulip</em> Species" Preprints. https://doi.org/10.20944/preprints202306.0008.v1
Abstract
Morphological features and composition of ISSR DNA fragments were studied in species of the genus Tulipa L. A comparative analysis of the data obtained was carried out and an attempt was made to clarify the species belonging of plants. Plants obtained from various sources were studied. It was found that representatives of the genus Tulipa L. from the two subgenera Tulipa and Eriostemones are well separated not only morphologically, but also by the composition of ISSR fragments. At the intra- and interspecific level, the results for morphological traits and molecular data differed. Interspecific and intraspecific differences were more clearly traced in the complex analysis of morphological features and ISSR PCR data. All samples obtained in the form of bulbs and renewed vegetatively had an identical set of ISSR fragments. Plants grown from seeds were characterized by a significant variety of molecular markers and had both species-specific and in-dividual genetic variability. It was found that the samples obtained and registered as the Tulipa urumiensis Stapf are the yellow-flowered form of the Tulipa tarda Stapf, and a sample received as the Tulipa turkestanika Regel is the Tulipa bifloriformis Vved. Thus, a comprehensive study of both the data of fragmentary DNA analysis and morphological features, provided a sufficient number of DNA samples, makes it possible to clarify the species of tulip plants, and also allows us to assess the genetic diversity of the genus Tulipa.
Keywords
Tulipa; tulip; species; identification; DNA; PCR; ISSR analysis; morphological features
Subject
Biology and Life Sciences, Horticulture
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
