Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biological Activity of Beauveria bassiana and Chemical Profile of Its Volatile Secondary Metabolites Using Spme-Gc/Ms Analysis

Version 1 : Received: 29 May 2023 / Approved: 30 May 2023 / Online: 30 May 2023 (09:02:51 CEST)

How to cite: Camele, I.; Sadeek, S.A.; Racioppi, R.; Elshafie, H.S. Biological Activity of Beauveria bassiana and Chemical Profile of Its Volatile Secondary Metabolites Using Spme-Gc/Ms Analysis. Preprints 2023, 2023052095. https://doi.org/10.20944/preprints202305.2095.v1 Camele, I.; Sadeek, S.A.; Racioppi, R.; Elshafie, H.S. Biological Activity of Beauveria bassiana and Chemical Profile of Its Volatile Secondary Metabolites Using Spme-Gc/Ms Analysis. Preprints 2023, 2023052095. https://doi.org/10.20944/preprints202305.2095.v1

Abstract

The genus Beauveria include important entomopathogenic and endophytic fungi, among them, Beauveria bassiana is the most studied species. However, there are few knowledge regarding their antimicrobial activity. The current research has been conducted to evaluate in vitro antagonistic activity of B. bassiana and the antimicrobial efficacy of its Exo and Endo metabolites against Bacillus cereus, B. megaterium, Clavibacter michiganensis (gram positive bacteria, G+ve), Xanthomonas campestris, Pseudomonas aeruginosa and P. fluorescence (gram negative bacteria, G-ve). In addition, Solid-phase microextraction (SPME) was coupled to Gas Chromatography-Mass Spectrometry (GC/MS) to qualitatively measure the volatile organic compounds (VOCs) metabolic profile of the most efficient studied isolate of B. bassiana. The obtained results showed that, the isolate UniB2439-3 has promising antibacterial effect against most of studied target bacteria. SPME-GC/MS analysis of VOCs revealed the presence of ethanol; butanal,2-methyl; 2,4-dimethyl-1-heptene; octane, 4-methyl and β-elemene as the main dominant bioactive compounds. The outgoing results explicated that the efficient isolate of B. bassiana can be potentially used as a biocontrol agent against several bacteria especially G+ve ones.

Keywords

biocontrol; natural products; phytopathogens; microbial metabolites; entomopathogens; endophytic fungi.

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

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