Wang, Q.; Xue, N.; Sun, C.; Tao, J.; Mi, C.; Yuan, Y.; Pan, X.; Gui, M.; Long, R.; Ding, R.; Li, S.; Lin, L. Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.. Genes2023, 14, 1396.
Wang, Q.; Xue, N.; Sun, C.; Tao, J.; Mi, C.; Yuan, Y.; Pan, X.; Gui, M.; Long, R.; Ding, R.; Li, S.; Lin, L. Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.. Genes 2023, 14, 1396.
Wang, Q.; Xue, N.; Sun, C.; Tao, J.; Mi, C.; Yuan, Y.; Pan, X.; Gui, M.; Long, R.; Ding, R.; Li, S.; Lin, L. Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.. Genes2023, 14, 1396.
Wang, Q.; Xue, N.; Sun, C.; Tao, J.; Mi, C.; Yuan, Y.; Pan, X.; Gui, M.; Long, R.; Ding, R.; Li, S.; Lin, L. Transcriptomic Profiling of Shoot Apical Meristem Aberrations in the Multi-Main-Stem Mutant (ms) of Brassica napus L.. Genes 2023, 14, 1396.
Abstract
Rapeseed (Brassica napus L.) is a globally important oilseed crop with various uses, including consumption of its succulent stems as a seasonal vegetable, but its uniaxial branching habit limits the stem yield. Therefore, developing a multi-stem rapeseed variety has become increasingly crucial. In this study, a nature mutant of wild type from germplasm resources with stable inheritance of the multi-stem trait (MS) was obtained and showed abnormal shoot apical meristem (SAM) development and increased main stem number compared to WT. Histological and scanning electron microscopy analyses revealed multiple SAMs in the MS mutant, while only a single SAM was found in WT. Compared to WT, the mutant exhibited increased accumulation of cytokinins (CKs), transcriptome and RT-qPCR analyses showed the expression of genes involved in CK biosynthesis and metabolism pathways were altered in MS mutant. These findings provide insight into the mechanism of multiple main stems formation in Brassica napus L. and lay a theoretical foundation for breeding multi-main stem rapeseed vegetable varieties.
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