Preprint Article Version 3 Preserved in Portico This version is not peer-reviewed

Concerns Raised on Blood Group Determinants in Plasma Membrane Interaction of the SARS-CoV-2

Version 1 : Received: 17 March 2021 / Approved: 18 March 2021 / Online: 18 March 2021 (07:38:11 CET)
Version 2 : Received: 23 March 2021 / Approved: 23 March 2021 / Online: 23 March 2021 (16:53:57 CET)
Version 3 : Received: 12 April 2021 / Approved: 12 April 2021 / Online: 12 April 2021 (14:31:31 CEST)

How to cite: Fiedler, K. Concerns Raised on Blood Group Determinants in Plasma Membrane Interaction of the SARS-CoV-2. Preprints 2021, 2021030460 (doi: 10.20944/preprints202103.0460.v3). Fiedler, K. Concerns Raised on Blood Group Determinants in Plasma Membrane Interaction of the SARS-CoV-2. Preprints 2021, 2021030460 (doi: 10.20944/preprints202103.0460.v3).

Abstract

The SARS-CoV-2 pandemic has resulted in the generation of evolutionary-related variants. The S-protein of the B.1.1.7 variant (deletion N-terminal domain (NTD) His69Val70Tyr144) may contribute to altered infectivity. These mutations may have been presaged by animal mutations in minks housed in mink farms that according to the present analysis by modelling of protein ligand docking altered a high affinity binding site in the S-protein NTD. These mutants likely occurred only sporadically in humans. Tissue-adaptations and the size of the mink relative to the infected human population size back then may have comparatively increased the relative mutation rate. Simple, multi-threaded automated docking that is widely available, assigns increased binding of the blood type II A antigen to the SARS-Cov-2 S-protein NTD of B.1.1.7 with an overall increased docking interaction of blood group A harbouring glycolipids relative to group B or H (H, p=0.04). The top scoring glycan is identified as a DSGG (also classified as sialosyl-MSGG or disialosyl-Gb5) that may compete with heparin, which is similar to heparan sulfate linked to proteinaceous receptors on the tissue surface. Other glycolipids are found to interact with lower affinity, except long ligands that have suitable ligand binding poses to match the curved binding pocket.

Keywords

Glycans; Coronaviridae; COVID-19; Organ Tropism

Subject

CHEMISTRY, Analytical Chemistry

Comments (2)

Comment 1
Received: 12 April 2021
Commenter: Klaus Fiedler
Commenter's Conflict of Interests: Author
Comment: The legends to Supplement Figures were mixed up and were ordered, it is stated, that the visibility of amino acids refers to display of Van der Waals radii of residues (see Supplement Figure).
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Comment 2
Received: 17 May 2021
Commenter:
The commenter has declared there is no conflict of interests.
Comment: Strikingly, cell-free DNA maps have recently alerted towards a significant proportion of erythroblast cf plasma DNA in COVID-19 patients, seemingly increased in severe disease (JCI Insight. 2021;6(7):e147610. https://doi.org/10.1172/jci.insight.147610 and Med 2, 411–422 April 9, 2021 https://doi.org/10.1016/j.medj.2021.01.001).
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