Preprint Article Version 1 This version is not peer-reviewed

Comparative Plastomics of Ashwagandha (Withania, Solanaceae) and Identification of Mutational Hotspots for Barcoding Medicinal Plants

Version 1 : Received: 10 March 2020 / Approved: 11 March 2020 / Online: 11 March 2020 (10:21:56 CET)

How to cite: Mehmood, F.; ., A.; Ubaid, Z.; Bao, Y.; Poczai, P.; Mirza, B. Comparative Plastomics of Ashwagandha (Withania, Solanaceae) and Identification of Mutational Hotspots for Barcoding Medicinal Plants. Preprints 2020, 2020030181 (doi: 10.20944/preprints202003.0181.v1). Mehmood, F.; ., A.; Ubaid, Z.; Bao, Y.; Poczai, P.; Mirza, B. Comparative Plastomics of Ashwagandha (Withania, Solanaceae) and Identification of Mutational Hotspots for Barcoding Medicinal Plants. Preprints 2020, 2020030181 (doi: 10.20944/preprints202003.0181.v1).

Abstract

Within the family Solanaceae, Withania is a small genus belonging to the Solanoideae subfamily. Here, we report the de novo assembled, complete, plastomed genome sequences of W. coagulans, W. adpressa, and W. riebeckii. The length of these genomes ranged from 154,198 base pairs (bp) to 154,361 bp and contained a pair of inverted repeats (IRa and IRb) of 25,027--25,071 bp that were separated by a large single-copy (LSC) region of 85,675--85,760 bp and a small single-copy (SSC) region of 18,457--18,469 bp. We analyzed the structural organization, gene content and order, guanine-cytosine content, codon usage, RNA-editing sites, microsatellites, oligonucleotide and tandem repeats, and substitutions of Withania plastid genomes, which revealed close resemblance among the species. Both the substitution and insertion and deletion analyses confirmed that the IR region was significantly conserved compared with the LSC and SSC regions. Further comparative analysis among the Withania species highlighted 30 divergent hotspots that could potentially be used for molecular marker development, phylogenetic analysis, and species identification.

Subject Areas

Ashwagandha; Chloroplast Genome; InDels; Medicinal plants; Mutational hotspots; Phylogenomics; Solanaceae; Substitutions; Withania

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