Preprint Review Version 1 This version is not peer-reviewed

Fast Neutron Mutagenesis in Plants: Advances, Applicability and Challenges

Version 1 : Received: 9 May 2019 / Approved: 10 May 2019 / Online: 10 May 2019 (14:38:21 CEST)

A peer-reviewed article of this Preprint also exists.

Kumawat, S.; Rana, N.; Bansal, R.; Vishwakarma, G.; Mehetre, S.T.; Das, B.K.; Kumar, M.; Kumar Yadav, S.; Sonah, H.; Sharma, T.R.; Deshmukh, R. Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement. Plants 2019, 8, 164. Kumawat, S.; Rana, N.; Bansal, R.; Vishwakarma, G.; Mehetre, S.T.; Das, B.K.; Kumar, M.; Kumar Yadav, S.; Sonah, H.; Sharma, T.R.; Deshmukh, R. Expanding Avenue of Fast Neutron Mediated Mutagenesis for Crop Improvement. Plants 2019, 8, 164.

Journal reference: Plants 2019, 8, 164
DOI: 10.3390/plants8060164

Abstract

Fast neutron (FN) radiation mediated mutagenesis is a unique approach among the several induced mutagenesis methods being used in plant science in terms of impacted mutations. The FN mutagenesis usually creates deletions from a few bases to several million bases (Mb). A library of random deletion generated using FN mutagenesis lines can provide indispensable resources for the reverse genetic approaches. In this review, information from several efforts made using FN mutagenesis has been compiled to understand the type of induced mutations, frequency, and genetic stability. Concerns regarding the utilization of FN mutagenesis technique for a plant with different level of ploidy and genome complexity are discussed. We have highlighted the utility of next-generation sequencing techniques which can be efficiently utilized for the characterization of mutant lines as well as for the mapping of causal mutations. Pros and cons of Mut-map, MutChromSeq, exon capture, whole genome sequencing, MutRen-Seq, and different tilling approaches can be used for the detection of FN-induced mutation has also been discussed. Genomic resources developed using the FN mutagenesis have been catalogued wooing to meaningful utilization of the available resources. The information provided here will be helpful for the efficient exploration for the crop improvement programs and for better understanding of genetic regulations.

Subject Areas

induced mutagenesis; fast neutron irradiation; mutation mapping; deletagene; next-generation sequencing technologies; reverse genetics

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.