Abstract: Hysteresis is normally unavoidable in hydrogels under complex external loading conditions due to the intermolecular friction, which usually leads to fatigue. Here, we develop a sarcomere-inspired double-network hydrogel made from polyacrylamide, alginate and phytic acid, whose hysteresis can be precisely modulated by preloading. Particularly, due to the synergy of micellization, fibrillation and micro-lubrication, the as-prepared hydrogel displays an ultra-low hysteresis (≤ 0.02 %) after it experiences a pre-tensile process at a specific amplitude and strain rate, or even possesses negative hysteresis in the case of low tensile amplitudes or high strain rates. Interestingly, smart responses of the developed hydrogel to cyclic tensile loadingare similar to the mechanical behaviors of sarcomeres in vivo. Likewise, the derived hydrogel with ultra-low hysteresis performs reliably even at temperatures as low as -20 ℃. The ultra-low hysteresis presented by the biomimetic hydrogel with ultra-low hysteresis makes it suitable for many engineering fields like electrical sensing with superior reliability (the corresponding electrical signal (ΔR/R0) is stable even after 1000 stretching-unstretching cycles). Moreover, the design strategy of hydrogels with programmable hysteresis provides an innovative methodology for the future development of smart high-performance hydrogels.

Abstract: Technology plays a vital role in the way teacher’s work, communicate, and share their knowledge particularly after COVID pandemic. Thus, it is a matter of great importance both from theoretical and practical point of view to understand the factors that govern knowledge sharing through technologies. This study integrates Teo’s composite model of Technology Acceptance(TA) and Knowledge Sharing(KS) construct of Van den Hooff & Van Weenen, to empirically examine the relationship between technology acceptance and knowledge sharing among teachers. A study used a descriptive-correlational cross-sectional research approach. 225 participants responded to the survey. The study used Technology Acceptance Questionnaire, Teo( 2011) with 20 items(α=0.84) and Knowledge Sharing Questionnaire by Van den Hooff and Van Weenen ( 2004), with 12 items(α=0.96). One- sample t-test was used to find out the degree to which Technology Acceptance and Knowledge Sharing is practised by teachers. Pearson’s correlation was used to identify if there is any positive relationship between Technology Acceptance components and Knowledge Sharing elements. Structural equation modelling (SEM) was used to study whether any of the factors of Technology Acceptance can significantly predict the two types of Knowledge Sharing.Perceived Usefulness (PU) and Facilitating Conditions (FC) emerged as the most influential factors of Technology Acceptance in driving teachers’ Knowledge Sharing.

Abstract: Coronary heart disease (CHD) is a leading cause of morbidity and mortality, driven by metabolic remodeling, vascular inflammation, and perivascular adipose tissue (PVAT) dysfunction. We integrated bulk transcriptomic datasets to develop a machine learning–based diagnostic model, evaluated 113 algorithms, and identified a seven-gene signature (PYGL, PTGS2, PFKFB3, MMP9, CYP1B1, CXCR1, ABCB1) with robust predictive performance. Single-cell RNA sequencing of coronary PVAT revealed substantial cellular heterogeneity and prioritized PFKFB3 as a hub linking glycolytic activity to NF-κB regulon activity. Macrophage-centered communication via SPP1, MIF, and other pathways was enhanced in disease conditions. Virtual knockout of PFKFB3 induced transcriptional changes enriched in immune activation, phagocytosis, and oxidative stress, while molecular dynamics simulations indicated stable salidroside binding within PFKFB3. Together, these analyses provide a multi-layered framework connecting glycolytic remodeling, inflammatory transcriptional activity, and intercellular signaling in CHD. The findings support PFKFB3 as a potential biomarker and mechanistic hub, and suggest that salidroside may modulate its activity. This study offers an integrative computational foundation for future experimental validation and mechanistic exploration of PVAT dysfunction in CHD.

Abstract: NHEJ is one of the preferred DNA-damage repairing mechanisms for human cells, due to its rapid onset and activity throughout the cell cycle. It is, nevertheless, error prone and highly relevant in numerous oncological malignancies. In the recent years, it gained a lot of attention as a target either for cancer treatment or synergetic strategies, as pharmacological- and radio-sensitization approaches. This study evaluated the genotoxicity and radiosensitization potential of three DNA-PK inhibitors (PIK-75, M3814, and CC-115) in breast cancer by comparing MDA-MB-231 (NHEJ reliant) and MCF-7 (NHEJ and HR reliant) cell lines. Cells were preincubated with the DNA-PK inhibitors, using four different concentrations either in monotherapy or in combination with a DNA damage-inducer (doxorubicin or [64Cu]CuCl2). Viability was measured by MTS assays at 24, 48, and 72 h, while the DNA damage by γH2AX and flow cytometry. DNA-PK blockade preferentially sensitized NHEJ-reliant breast cancer cells to doxorubicin and 64Cu effect. Combination treatments generally reduced viability relative to inhibitor’s monotherapy, with clearer concentration dependence and stronger effects in MDA-MB-231. In the case of [64Cu]CuCl2 alone, MDA-MB-231 viability was reduced to 70–75% at 24 h, whereas several inhibitor combinations reduced it further; MCF-7 showed weaker or inconsistent potentiation. Delayed cytotoxicity was most obvious at 72 h, showing persistence of unrepaired DNA damage after DNA-PK inhibition. Overall, the results highlight the potential of exploiting repair-pathway dependence to improve targeted radiotherapy in breast cancer.

Abstract: The introduction of sustainable practices into waste management can have favorable environment impact, increase resource value, and economic gains. Hydrothermal technologies have strong potential for the production of up-cycled ingredients from biowaste (amino acids, sugars, phenols, pharmacologically-active compounds, etc.), enabling additionally high energy recovery (50-80%) from biowaste with net-negative carbon emission. This review discusses the use of subcritical and supercritical water technologies for sustainable valorization of biowaste and conversion of biomass into high-value chemicals and biofuels. The potential for the extraction/generation of bioactive compounds from plant and animal waste is presented, emphasizing the efficiency, compound stability, and bioactivity of fractions obtained. The possibilities of simultaneous extraction of added-value compounds and hydrolysis of feedstock biopolymers by said technologies are elaborated. The review further addresses the production of biofuels through hydrothermal carbonization for solid fuels, hydrothermal waste liquefaction for liquid fuels, and supercritical water gasification for gaseous fuels. The paper highlights the environmental and economic advantages of technologies based on sub- and supercritical water, over conventional chemical and fermentative routes, emphasizing their contribution to circular bioeconomy by converting biowaste into value-added products and sustainable energy sources.

Abstract: Cycling is one of the most popular sports and recreational activities. Millions of new people start to integrate bicycling into their daily routines every year. Fitness and activity trackers are the most powerful motivation tools for cycling novices and serious cycling enthusiasts. For this purpose, we present LaserFit, a laser-based direct force power meter for fitness and activity tracking during cycling. We developed embedded hardware to collect the torque and the wheel rotation data, which is produced by a laser-based position sensing system mounted on the rear wheel to precisely record the power output produced by the rider during cycling. The sensor data transmits to a smartphone via Bluetooth/ANT+ for data acquisition and analysis. Our device can be produced at low costs and deliver a level of accuracy similar to that obtained with the most expensive systems available on the market. To evaluate the accuracy of our system extensive experiments were conducted. The results of the present study suggest that the LaserFit power meter provides a strong relationship (r = 0.97) across a range of trials in laboratory and field conditions when compared with the SRM power meter. The LaserFit is therefore considered a valid alternative for training and performance measurement during continuous cycling.

Abstract: Antibody–drug conjugates (ADCs) are a pivotal technology for precision cancer therapy, harnessing the synergistic effects of antibody targeting and toxin delivery. However, traditional ADCs encounter limitations in efficacy that stem from tumor resistance, heterogeneity, and intense target competition. Dual-payload ADCs (DP-ADCs) represent a promising solution to these challenges, as they leverage dual mechanisms of action that mitigate acquired drug resistance and enhance adaptability to tumor heterogeneity. The complex structure of DP-ADCs presents substantial quality control hurdles. In this manuscript, we review the current payload selection and conjugation strategies of DP-ADCs and examine recent advances in quality control research. Specifically, we analyze the analytical challenges related to the quantification of free toxins, the determination of the total antibody content, and the characterization of the drug-to-antibody ratio and its distribution. Ultimately, the aim of this work is to provide valuable guidance for future DP-ADC quality control analyses to facilitate their clinical translation and application.

Abstract: Lignin-cellulose mixtures (LCMs) generated as intermediates in wood biorefineries are commonly separated into lignin and cellulose. However, using ultrasound (US) to pro-cess these mixtures could create novel, valuable materials not possible with conven-tional methods. This study looked at how lignin affects the US modification of these mixtures. Crude and partially delignified LCMs were successfully prepared using aqueous solutions of EtOH, THF and dilute NaOH and then subjected to short, high-power US treatment. The resulting materials were characterised using FT-IR spectroscopy, particle size analysis, water retention value analysis, SEM and XRD. Sonication rapidly reduced the mean particle size, generating cellulose nanofibril-like structures in all samples according to SEM. The response depended strongly on lignin content, with samples containing lower amounts of lignin exhibiting substantially higher hydration capacity and stronger US responsiveness. At the molecular level, lig-nin removal exposes cellulose surfaces and enhances hydrophilic interface formation, increasing water uptake and suspension stability. Thus, results show that lignin limits accessible hydrophilic cellulose surface area rather than preventing fragmentation by sonication. US is therefore a chemical-lean strategy to tune the physicochemical prop-erties of partly delignified LCMs and expand the product portfolio of integrated wood biorefineries towards novel advanced lignocellulosic materials.

Abstract: This study analyzes the relationship between digital access and knowledge-economy practices among young Ecuadorian adults, with emphasis on their implications for sustainable digital inclusion and knowledge-based development. The study is based on the premise that access to the Internet, devices, and technological tools does not necessarily ensure critical, productive, collaborative, or knowledge-generating uses of information. A quantitative, non-experimental, cross-sectional, and descriptive-correlational design was applied to a sample of 441 young Ecuadorian adults aged 18 to 30. Data were collected through an online questionnaire and analyzed using descriptive statistics, reliability analysis, KMO and Bartlett indicators, the Mann–Whitney U test, effect sizes, and Spearman’s rho with 95% confidence intervals. The instrument showed very high internal consistency for knowledge-economy practices (α=.978; ω=.978) and digital access and technological resources (α=.963; ω=.964). The KMO values were also adequate for both variables (.963 and .902, respectively), and Bartlett’s tests were statistically significant (p<.001). The results showed that digital access received more favorable ratings than knowledge-economy practices. A very strong, positive, and statistically significant association was found between digital access and knowledge economy practices (ρ=.822, 95% CI [.765, .870], p<.001). Information management and collaboration was the dimension most strongly associated with digital access (ρ=.820, 95% CI [.764, .868], p<.001). Women reported higher scores than men in knowledge-economy practices, although the effect size was small (r=.158; rrb=.184). These findings suggest that digital access is a necessary but insufficient condition for sustainable digital inclusion. The study contributes empirical evidence from a developing-country context and highlights the need for educational and public-policy strategies that transform connectivity into critical learning, collaboration, innovation, and knowledge creation.

Abstract: This prospective case series evaluated the short-term outcomes following percutaneous cementoplasty as the sole palliative treatment for appendicular osteosarcoma in 10 dogs. Synthetic self-hardening calcium-phosphate bone substitute was injected into the osseous defect under fluoroscopic guidance after curettage of the bone tumor. Clinician assessment included a numerical rating score for lameness, offloading, and ease of lifting the contra-lateral limb as well as the 4A-VET post-operative pain scale. Owner assessment was obtained using three descriptive questionnaires, the Helsinki Chronic Pain Index (HCPI), the Canine Brief Pain Inventory (CBPI) and the Canine Symptom Assessment Scale (CSAS). Measures were recorded preoperatively and at 2-, 4-, 8-, and 12-weeks following surgery. Early improvement in the 4A-Vet score was noted at the 2-, 4-, 8-, and 12-week time points for all major pain and function metrics. Similarly, the CBPI pain severity and interference scores demonstrated early postoperative improvement during the 2- and 4-week time points with partial attenuation by 8- and 12-weeks. Panting, difficulty sleeping, whining/moaning, and lack of appetite were significantly reduced when assessed via the CSAS. Cementoplasty as a monotherapy, affording early pain relief and improved structural integrity, supports its role as a limb-sparing option for dogs unable to undergo amputation.

Abstract: The accelerating accumulation of atmospheric carbon dioxide (CO₂) from fossil fuel combustion represents one of the foremost environmental challenges of the twenty-first century. This paper presents the design, theoretical basis, and experimental framework of a novel artificial photosynthesis system capable of capturing CO₂ from combustion flue gases and converting it into oxygen (O₂) and energy-rich compounds, directly mimicking the biochemical process performed by trees. The proposed system integrates a sodium carbonate (Na₂CO₃) absorption tower for CO₂ capture, a thermal desorption unit for solvent regeneration, and a cobalt oxide-catalyzed photosynthetic reactor for CO₂-to-O₂ conversion. System performance is quantified using non-dispersive infrared (NDIR) sensors for CO₂ measurement and electrochemical oxygen sensors for O₂ detection. Stoichiometric analysis indicates that 1 kg of captured CO₂ yields approximately 0.73 kg of O₂, and national-scale deployment projections suggest energy savings of approximately $200 billion per year by 2030 alongside a potential reduction of 302,600 million metric tons of CO₂ emissions. Comparative analysis with existing decarbonization approaches—including carbon capture and storage (CCS), hydrogen production, and enhanced oil recovery (EOR)—demonstrates that artificial photosynthesis offers a fundamentally superior outcome by permanently transforming CO₂ into life-sustaining O₂ rather than merely sequestering or displacing it. This work establishes a laboratory-scale proof of concept and a systematic experimental roadmap for scaling the technology to industrial application.

Abstract: The growing demand for high-efficiency, high-power-density converters in data centers, electric vehicle chargers, and renewable energy systems has accelerated the adoption of wide bandgap (WBG) devices. Gallium nitride (GaN) transistors offer superior switching speed, lower losses, and higher power density compared with Silicon (Si) devices. Accurate characterization of GaN switching dynamics is essential due to parasitic effects and transient phenomena affecting performance and reliability. The Double Pulse Test (DPT) is widely used to quantify critical parameters, including switching energy losses, dynamic RDS(on) and transient voltage and current waveforms. This paper reviews DPT techniques for GaN devices, focusing on measurement methodologies, parasitic mitigation, and reliability considerations, providing practical guidance for optimizing high-frequency GaN-based power converters.

Abstract: Expanding renewable energy capacity in sub-Saharan transmission systems is a cornerstone of sustainable development, yet weak grid infrastructure and the absence of flexible storage remain principal barriers to reliable and low-carbon energy access. This paper addresses the economic and environmental dimensions of that challenge by proposing a hierarchical multi-objective framework for the optimal siting and sizing of Battery Energy Storage Systems (BESS), applied to the 130-bus Mali transmission network within the EMERGE project. The upper level employs the NSGA-II evolutionary algorithm to simultaneously maximize daily price-arbitrage revenue—the economic sustainability indicator—and minimize active power losses—the environmental efficiency indicator. For each candidate design, the lower level solves a multi-period DC Optimal Power Flow (DC-OPF) via CasADi/IPOPT, with thermal branch constraints embedded as hard linear inequalities through the Power Transfer Distribution Factor (PTDF) matrix, and voltage-corrected loss estimates recovered via a vectorized Extended DC Power Flow (EDCPF) model. Over 500 NSGA-II generations, the framework identifies Bus 91 (SIRAKORO II, 150 kV) as the dominant storage location, achieving maximum daily revenue of approximately € 10,033 at a marginal loss increment of 6.7×10⁻³ MWh. The Pareto front provides Mali system planners with a quantitative tool for balancing private investment returns against grid-level environmental impact, demonstrating that rigorous network-constrained BESS planning is both technically tractable and economically viable in the resource-constrained context of sub-Saharan sustainable energy transitions.

Abstract: This study evaluates the feasibility of using asphalt concrete as an impermeable core material for rockfill dams under tropical conditions. Laboratory testing and numerical modeling were conducted to assess the hydraulic and mechanical performance of asphalt concrete mixtures produced with locally available aggregates in Thailand. Asphalt mixtures were designed using the Marshall method with asphalt binder contents of 6% and 7% and target air void contents between 1-4%. Laboratory testing included permeability testing, Marshall stability testing, and triaxial compression tests to determine hydraulic conductivity, shear strength parameters, and deformation characteristics. Results show that asphalt concrete mixtures with air void contents below 1% exhibit extremely low permeability, with hydraulic conductivity on the order of 10⁻¹¹–10⁻¹² m/s, satisfying requirements for impervious dam cores. Triaxial compression tests yielded cohesion values between 97-572 kPa and friction angles ranging from 31° to 52°, indicating adequate shear resistance. Numerical simulations performed using GeoStudio compared rockfill dams with asphalt concrete cores and conventional clay cores. The results demonstrate that a 0.5‑m‑thick asphalt concrete core provides comparable seepage control and slope stability while requiring significantly smaller material volume. The findings suggest that asphalt concrete cores represent a technically feasible and economically advantageous alternative to clay cores, particularly in regions where suitable clay materials are limited.

Abstract: Walking is not merely locomotion but a window into the nervous system, integrating cortical, subcortical, cerebellar, spinal, and peripheral networks into a unified motor behavior. Across neurological diseases—including Parkinson’s disease, atypical parkinsonism, cerebellar ataxias, stroke, multiple sclerosis, neuropathies, neuromuscular disorders, and functional gait syndromes—gait disturbances are among the most disabling clinical features, contributing to falls, loss of independence, institutionalization, and premature mortality. Traditional bedside observation remains indispensable, but it lacks the sensitivity and reproducibility needed to capture subtle, episodic, or prodromal abnormalities. Over the past decade, advances in wearable sensors, marker-based and markerless motion capture, pressure-sensitive walkways, force plates, artificial intelligence, and machine learning have positioned digital mobility outcomes as promising, ecologically valid biomarkers of neurological function. These measures can support differential diagnosis, provide prognostic information on falls and survival, and serve as sensitive endpoints in therapeutic trials. They may also detect early abnormalities, such as increased stride-to-stride variability or prolonged double-support time, before overt clinical deterioration becomes evident. Clinical applications are increasingly evident across disorders, including distinguishing Parkinson’s disease from atypical parkinsonism, quantifying treatment response in normal-pressure hydrocephalus, tracking progression in ataxia and multiple sclerosis, predicting functional decline in motor neuron disease, and guiding rehabilitation after stroke. Integration with neuroimaging, electrophysiology, and molecular biomarkers is beginning to reveal the circuits underlying variability, instability, and freezing, positioning gait as a systems-level marker of neural integrity. Nevertheless, methodological heterogeneity, limited disease-specific validation, insufficient longitudinal data, and lack of consensus on clinically meaningful parameters continue to constrain translation. Cognitive, affective, and environmental influences also remain insufficiently represented in digital frameworks, while equity, accessibility, algorithmic bias, and privacy require careful ethical governance. Reconceptualizing gait as a “sixth vital sign” reframes mobility as a multidimensional biomarker of neural and systemic health. With harmonized protocols, robust validation, multimodal integration, and appropriate ethical frameworks, gait analysis could become a cornerstone of precision neurology.

Abstract: Moisture content is a critical parameter influencing the durability, structural performance, and maintenance of timber structures. However, current building inspection practices often rely on subjective interpretation, resulting in inconsistent assessment outcomes and ineffective maintenance decision-making. Despite the availability of various moisture measurement techniques, a standardized framework for interpreting moisture levels in relation to timber condition is still lacking. This study presents a structured review and synthesis of moisture content thresholds reported in the literature and proposes a standardized classification framework for timber defect assessment. The findings indicate that moisture content levels can be systematically categorized into four condition states dry, moderate, poor, and critical for each associated with specific maintenance actions. The proposed framework provides a practical linkage between moisture measurements and condition-based maintenance strategies, enabling more consistent and reliable inspection practices. The study contributes by transforming dispersed moisture-related data into a unified and actionable classification system, serving as a decision-support tool for building inspectors and maintenance practitioners. The proposed framework enhances the implementation of condition-based maintenance by reducing subjectivity and improving the accuracy of timber condition assessment.

Abstract: Rainfall and groundwater fluctuations may influence the migration or dilution of contaminants from US Superfund sites. This pilot study was designed to provide empirical data on relationships between rainfall and contaminant concentrations in water near Superfund sites. Four Superfund sites on Long Island, New York were selected to represent a range of contaminant profiles and hydrogeologic conditions. Baseline samples were collected in December 2022, with subsequent samples obtained following major storms and seasonally through September 2023. Samples were collected on 11–24 different days across the sites and measured for metals and 1,4-dioxane. Overall, we observed few consistent associations between rainfall and contaminant concentrations across sites. Two trends, however, were noted: (1) At Gowanus Canal, a surface water site, there was a negative association between rainfall and contaminant concentrations using same-day or prior-day rainfall; and (2) in a treatment well near Lawrence Aviation, negative associations were observed between prior month’s rainfall and contaminant concentrations. Rainfall appeared to dilute contaminant concentrations at two of the four Superfund sites. Given the increased frequency and intensity of extreme rainfall events, it is important to continue probing links between rainfall and contaminants near Superfund sites.

Abstract: Agentic artificial intelligence systems increasingly combine language models, memory, retrieval, tool use, orchestration, and human oversight. For software engineering, this creates a variability problem that feature-oriented product line methods only partly address: organizations are configuring not only functions or components, but permitted patterns of agency. Unlike MAS-SPL, which mainly structures families of agent roles and interactions, the proposed approach targets LLM-based agents whose prompts, retrieval sources, tool authority, runtime monitoring, and governance boundaries vary together. This paper proposes Agency-Centric Product Line Engineering for governed agentic AI systems. It defines agency variability as systematic variation in a system's capacity to pursue goals, perceive context, reason and plan, use tools, exercise authority, interact with humans or other agents, remain observable, and evolve under governance constraints. It also defines semantic observability as runtime monitoring of whether semantic behavior—goals, tool choices, evidence use, proposed actions, and escalation decisions—remains consistent with the granted agency profile. The paper contributes a nine-dimension Agency Variability Model, an Agency Configuration Schema, a constraint-based validation algorithm, and a prototype-style derivation of portfolio-management agent variants. The case shows that five variants can share assets while differing in perception, authority, autonomy, human control, and topology.

Abstract: The hexane extract of Psoralea drupacea Bunge fruits was initially evaluated for antivi-ral activity against SARS-CoV-2 based on GC-MS data indicating high bakuchiol con-tent (87.74%). Unexpectedly, the extract showed no antiviral effect in Vero E6 cells due to cytotoxicity (CC₅₀ = 7.5 μg/mL), while purified bakuchiol demonstrated moderate antiviral activity (IC₅₀ = 6.2 ± 0.8 μg/mL; SI = 2.9). Quantitative NMR revealed that the actual bakuchiol content in the extract was 44.3% — approximately half the GC-MS value — explaining the lack of efficacy at non-cytotoxic concentrations. Both the ex-tract and purified bakuchiol effectively blocked the RBD-ACE2 interaction in a com-petitive ELISA (71.3% inhibition at 50 μM for bakuchiol; IC₅₀ = 18.5 μM). Notably, the extract also inhibited the viral main protease 3CLpro (IC₅₀ = 32.0 ± 3.5 μg/mL), while purified bakuchiol showed no such activity. These findings reveal a dual mechanism: bakuchiol inhibits viral entry via RBD-ACE2 blockade, while other extract components (e.g., angelicin, psoralen) suppress viral replication via 3CLpro inhibition.

Abstract: Background and Objective: Treat-and-extend(TREX) dosing is widely used for anti-VEGF therapy in macular disease but no principled basis exists for choosing interval-change rules. We frame TREX as a threshold-search problem and compare rules in terms of search efficiency and overshoot.Patients and Methods: A deterministic model represented TREX as a threshold search for the true maximum dry interval(T_max) on a 1-week grid(4–16 weeks). Four rules were evaluated:+2/−2,+4/−2,+4/−4 with midpoint refinement, and a midpoint binary-like rule. Visits to maintenance interval and overshoot metrics were calculated for each rule and T_max.Results: No single rule was optimal. The +2/−2 rule minimised overshoot (maximum 2 weeks) but required most visits. The +4/−2 rule was fastest. In long-durability eyes, midpoint search matched +4/−2 in speed with overshoot comparable to +2/−2.Conclusion: Framing TREX as a threshold search makes the speed-overshoot trade-off explicit and provides a principled basis for choosing and justifying interval-change rules.