This study aimed to characterize the antioxidant, mechanical and physical properties of chicken skin gelatin/CMC/Centella asiatica blended film. The influence of Centella asiatica at 0.3% and 0.7% on antioxidant activities; mechanical properties and physical properties of chicken skin gelatin/CMC/Centella asiatica film were investigated. Characterization of the blended films with 0.7% Centella asiatica extract shows higher antioxidant activities with a total phenolic content of 0.36 mg/g of GAE, DPPH of 89.26%, and reducing power of 0.80 nm compared to 0.3% Centella asiatica extract. The addition of 0.3% of Centella extract provide higher value in tensile strength (5.0 × 10⁻² MPa), elongation at break (281%), melting point (131.31 °C), transparency (0.86) but lower UV-light penetration. While the addition of 0.7% Centella extract contribute to higher value in WVP (1.13 × 10⁻⁴ g m⁻¹s⁻¹Pa⁻¹) and puncture test (0.06 N). There are no significant differences between functional groups obtained from this blended film as evaluated by FTIR analysis (p > 0.05). Furthermore, XRD analysis showed the addition of extract decrease the crystallinity of film. In conclusion, the incorporation of Centella asiatica extracts on film greatly increased antioxidant levels and improved some of the mechanical and physical properties of the film blends.

Keywords: mHealth; peritoneal dialysis; functionality; usability

Based on the principle of Pancharatnam-Berry phase, we propose an approach to construct dual-functional dielectric metasurface doublets. This type of doublet, which is composed of a substrate and two metasurface layers, performs two different functionalities at two different operating wavelengths. The simulated results of the examples show that the designed dual-functional doublet works well as expected. The proposed approach can be used to design dielectric meta-structures with more layers of meta-surface and thereby with more functionalities, and would be of interest for miniaturization and integration.

Saponins are a diverse group of naturally occurring plant secondary metabolites present in a wide range of foods ranging from grains, pulses, green leaves to sea creatures. They consist of a hydrophilic sugar moiety linked to a lipophilic aglycone, resulting in an amphiphilic nature and unique functional properties. The amphiphilic structures enable saponins to exhibit surface-active properties, forming stable foams and complexes with various molecules. In the context of food applications, saponins are utilized as natural emulsifiers, foaming agents, and stabilizers. They contribute to texture and stability in food products and have potential health benefits, including cholesterol-lowering and anticancer effects. Saponins possess additional bioactivities that make them valuable in the pharmaceutical industry as anti-inflammatory, antimicrobial, antiviral, and antiparasitic agents to name a few. Saponins can demonstrate cytotoxic activity against cancer cell lines and can also act as adjuvants, enhancing the immune response to vaccines. Their ability to form stable complexes with drugs further expands their potential in drug delivery systems. However, challenges such as bitterness, cytotoxicity, and instability under certain conditions need to be addressed for effective utilization of saponins in foods and related applications. In this paper, we have reviewed chemistry, functionality, and application aspects of saponins from various plant sources and have summarised the regulatory aspects of food application of Quillaja saponins. Further research to explore the full potential of saponins in improving food quality and human health has been suggested. It is expected that this article will be a useful resource for researchers in food, feed, pharmaceutical and material science.

In this investigation magnetic γ-Al2O3 ceramic nanocomposite particles bearing epoxide functionality are prepared following a multistep process. The ultimate nanocomposite particles are named as γ-Al2O3/Fe3O4/SiO2/poly(glycidyl methacrylate (PGMA). The surface property is evaluated by carrying out the adsorption study of Remazol navy (RN), a model reactive azo dye, on both γ-Al2O3/Fe3O4/SiO2 and γ-Al2O3/Fe3O4/SiO2/PGMA nanocomposite particles. The adsorption is carried out at the point of zero charge (PZC) to neutralize the effect of particle surface charge. The adsorption rate is very fast, reached equilibrium (qe) value within five min. Due to mesoporous structure of silica layer γ-Al2O3/Fe3O4/SiO2 nanocomposite particles possessed relatively higher specific surface area and magnitude of adsorption is dependent on the total specific surface area. The introduction of epoxide functionality favored high adsorption capacity in mass per unit surface area. The adsorption process strictly followed Langmuir model. Thermodynamic equilibrium parameters implied that irrespective of surface functionality the adsorption process is spontaneous and exothermic. Pseudo-second-order rate kinetic model is more appropriate to explain the adsorption kinetics.

Background: Nursing home residents (NHRs) have special needs and may experience reduced functionality, higher sedentary behavior time (SBT) and decreased physical activity (PA). This study aimed to characterize NHRs based on SBT, PA, and functional performance (FP), and to identify associations between these factors. Methods: Cross-sectional study with 65 NHRs aged ≥60, evaluated by accelerometer (SBT, PA) and the following tests: Timed Up and Go (TUG), Short Physical Performance Battery (SPPB), Barthel Index (BI), and Mini-Mental State Examina-tion (MMSE). Health-related variables and anthropometry were assessed. The FP (TUG, SPPB, BI), SBT, PA intensities were used for K-means clustering analysis. Results: The cluster analysis identified: C1 – better FP, shorter SBT, and longer time in PA intensities; C2 - lower FP, longer SBT, and shorter time in PA. In addition, C2 were older, with lower MMSE, lower body mass index, and walking aids use. The SBT showed a negative weak correlation with BI. The moderate PA (MPA) was moderately correlated with the SPPB and strongly correlated with the TUG and BI. Conclusions: MPA is strongly associated with better FP and independence. NHRs have dif-ferent physical and cognitive profiles, which indicates the need for personalized strategies to prevent disability and increase PA.

In this work the seismic resilience of the Emergency Department of a real Hospital complex, located in Tuscany (Italy), has been quantified, with special reference to its nonstructural components and organizational features. Special attention has been paid to the ceilings, whose importance stood out in past earthquakes. A comprehensive meta-model has been set, able to relate all the considered parameters to the assumed response quantity, i.e. the waiting time of the red-code patients arriving to the Emergency Department in the hours immediately after the seismic event. The seismic resilience of the Emergency Department has been measured with reference to earthquakes compatible to the seismic hazard of the area.

Hyperacusis, i.e., an increased sensitivity to sounds, is described in several neurodevelopmental disorders (NDDs), including Fragile X Syndrome (FXS). The mechanisms underlying hyperacusis in FXS are still largely unknown and effective therapies are lacking. Big conductance calci-um-activated potassium (BKCa) channels have been proposed as a therapeutic target to treat several behavioral disturbances in FXS preclinical models, but their role in mediating their au-ditory alterations has not been specifically addressed. Furthermore, studies on the acoustic phe-notypes of FXS animal models have mostly focused on central rather than peripheral auditory pathways. Here we provided an extensive characterization of the peripheral auditory phenotype of the Fmr1-knockout (KO) mouse model of FXS at adulthood. We also assessed whether the acute administration of Chlorzoxazone, a BKCa agonist, could rescue the auditory abnormalities of mutant mice. Fmr1-KO mice both at 3 and 6 months showed a hyperacusis-like startle pheno-type with paradoxically reduced auditory brainstem responses associated with a loss of ribbon synapses in the inner hair cells (IHCs). BKCa expression was markedly reduced in the IHCs of KO mice, but only at 6 months, when Chlorzoxazone rescued mutant auditory dysfunction. Our findings highlight the age-dependent and progressive contribution of peripheral mechanisms and BKCa channels to hyperacusis in FXS, suggesting a novel therapeutic target to treat auditory dysfunction in NDDs.

Machine learning has become widely adopted as a strategy for dealing with a variety of cybersecurity issues, ranging from insider threat detection to intrusion and malware detection. However, by their very nature, machine learning systems can introduce vulnerabilities to a security defence whereby a learnt model is unaware of so-called adversarial examples that may intentionally result in mis-classification and therefore bypass a system. Adversarial machine learning has been a research topic for over a decade and is now an accepted but open problem. Much of the early research on adversarial examples has addressed issues related to computer vision, yet as machine learning continues to be adopted in other domains, then likewise it is important to assess the potential vulnerabilities that may occur. A key part of transferring to new domains relates to functionality-preservation, such that any crafted attack can still execute the original intended functionality when inspected by a human and/or a machine. In this literature survey, our main objective is to address the domain of adversarial machine learning attacks and examine the robustness of machine learning models in the cybersecurity and intrusion detection domains. We identify the key trends in current work observed in the literature, and explore how these relate to the research challenges that remain open for future works. Inclusion criteria were: articles related to functionality-preservation in adversarial machine learning for cybersecurity or intrusion detection with insight into robust classification. Generally, we excluded works that are not yet peer-reviewed; however, the authors include some significant papers that make a clear contribution to the domain. There is a risk of subjective bias in the selection of non-peer reviewed articles; however, this is mitigated by co-author review. We selected the following databases with a sizeable computer science element to search and retrieve literature: IEEE Xplore, ACM Digital Library, ScienceDirect, Scopus, SpringerLink,Google Scholar. The literature search was conducted upto January 2022. We have striven to ensure a comprehensive coverage of the domain to the best of our knowledge. We have performed systematic searches of the literature, noting our search terms and results, and following up on all materials that appear relevant and fit within the topic domains of this review. This research was funded by the Partnership PhD scheme at the University of the West of England in collaboration with Techmodal Ltd.

Asthma is a chronic respiratory disease that impairs breathing. Management of asthma presents a significant challenge due to its inherent variability; that is, its symptoms can substantially differ among individuals, thereby complicating the prediction and management of exacerbations. Furthermore, individuals with asthma often have unique triggers that precipitate symptoms or attacks. The identification of these triggers can often prove to be a challenging, and at times, an impractical attempt. To address this, our research proposes a practical, personalized alert system, predicated on individual lung function tests conducted under varying environmental conditions classified by air-quality sensors. To validate this concept, we conducted an observational pilot study involving healthy individuals. We recruited twelve healthy participants and monitored their responses across a broad spectrum of environments, characterized by varying air quality, temperature, and humidity conditions. The lung function for each participant, assessed using peak expiratory flow (PEF) values, was recorded in each of these environments. Our results highlighted substantial variability in pulmonary responses to different environments. Utilizing these insights, we proposed a personalized alarm system that provides real-time air-quality monitoring and issues alerts when environmental conditions may potentially become unfavorable. We also explored the feasibility of employing basic machine learning techniques to predict PEF values in the aforementioned environmental conditions. This proposed system has the potential to empower individuals in actively safeguarding their respiratory health and mitigating discomfort caused by environmental conditions, especially in cases of asthma patients. By enabling timely and personalized interventions, the system aims to provide individuals with the necessary tools to minimize exposure to asthma’s possible triggers.

Green Infrastructures (GI) are considered key to reconcile ecological and social benefits by providing multiple functions. The concept is increasingly promoted and guidelines for its implementation have been developed in many countries and regions of the Western Hemisphere. However, for other parts of the world, especially for countries with less developed infrastructures, promotion, guidance for decision-making and manuals for GI are often lacking. But the state of infrastructure development and often unplanned character of settlements in the Global South differ and result in specific constraints as well as demands to GI that need to be addressed explicitly. This study presents a methodological approach to explicitly address the specific conditions and physical limitations to GI development in urban areas of the Global South. A four step methodology was developed to assess the implementation potential for retrofitted and multifunctional urban green infrastructure in public areas. An initial site analysis (1) and the definition of design criteria as well as general strategies (2) to achieve the different dimensions of multi-functionality are the basis to derive spatial typologies (3) for GI elements and finally the spatial suitability assessment for potential placements (4). An application of the methodology to a study area in the metropolitan region of San José, Costa Rica, shows exemplarily that the potential to improve the hydrological conditions (up to 34% of surface runoff reduction), ecological conditions (increase of green space by 2,2 %, creation of 1500 m length of roadside greenery and two new habitat types), and social conditions (2.200 m of road type upgrading) of multi-functionality of the site through Green Infrastructures. These assessment results of different multi-functionality dimension can serve as a guidance for GI promotion and implementation in urban areas of the Global South.

COVID-19 patients can recover with a median SARS-CoV-2 clearance of 20 days post initial symptoms (PIS). However, we observed some COVID-19 patients with existing SARS-CoV-2 for more than 50 days PIS. This study aimed to investigate the cause of viral clearance delay and the infectivity in these patients. Demographic data and clinical characteristics of 22 long-term COVID-19 patients were collected. SARS-CoV-2 nucleic acid, peripheral lymphocyte count, and functionality were assessed. SARS-CoV-2-specific and neutralization antibodies were detected, followed by virus isolation and genome sequencing. The median age of the studied cohort was 59.83±12.94 years. All patients were clinically cured after long-term SARS-CoV-2 infection ranging from 53 to 112 days PIS. Peripheral lymphocytes counts were normal. Interferon gamma (IFN-ƴ)-generated CD4+ and CD8+ cells were normal as 24.68±9.60% and 66.41±14.87%. However, the number of IFN-ƴ-generated NK cells diminished (58.03±11.78%). All patients presented detectable IgG, which positively correlated with mild neutralizing activity (ID50=157.2, P=0.05). SARS-CoV-2 was not isolated, and a cytopathic effect was lacking. Only three synonymous variants were identified in spike protein coding regions. In conclusion, decreased IFN-γ production by NK cells and low neutralizing antibodies might favor SARS-CoV-2 long-term existence. Further, low viral load and weak viral pathogenicity was observed in COVID-19 patients with long-term SARS-CoV-2 infection.

The interest in high homogenization pressure technology has grown over the years. It is a green technology with low energy consumption, not generating high CO2 emissions or polluting effluents. The main food applications derive from its effect on particle size, causing a more homogeneous distribution of fluid elements (particles, globules, droplets, aggregates, etc.) and favouring the release of intracellular components; and its effect on the structure and configuration of chemical components such as polyphenols and macromolecules such as carbohydrates (fibres) and proteins (also microorganisms and enzymes). The challenges of the 21st century lead food industry processing towards obtaining food with high nutritional quality and taking advantage of waste to obtain ingredients with specific properties. For this purpose, soft and non-thermal technologies such as high pressures homogenization have a huge potential. The objective of this work is to review how the need to combine safety, functionality and sustainability in food industry has conditioned the last decade applications of high-pressure homogenization technology.

The evolution of human societal dynamics over the course of decades and centuries has led to an ever-increasingly complex digital modern life, one of the hallmark of which is the need for multi-tasking, inclusive of that with media. Is there an empirical mapping between this macroscopic (digital) evolution of the human societal dynamics (in terms of multi-tasking) and the microscopic evolution of the functional molecular units within cells (i.e., proteins) and their manifold cross-talks? Multi-tasking requires a certain degree of intrinsic or adapted flexibility which is as true at the bio-molecular level. Evolutionary diversification of structure-function relationship in proteins emphasizes the functional importance of intrinsically disordered entities (IDPs/IDRs) which are highly dynamic biological soft matters. Multi-functionality is favorably supported by their fluid-like shapes and other new malleable members of the protein family. The new wealth of knowledge coming from recent research on protein intrinsic disorder, fold-switching, moonlighting, hub proteins etc. has given us new insights into protein structure – functional relationship. This has led to a paradigm shift in protein science, clearly diverging from the traditional 'one structure – one function' model applicable to enzyme classes to a more complex understanding of protein functionality. This paradigm shift has caused scientists to delve deeper into the subject, exploring various evolutionary toys and tools to fit the cumulative multi-functional demands in higher organisms. This commentary covers the different evolutionary arsenals to achieve the growing multi-functionality and argues in favor of protein intrinsic disorder as probably the sharpest weapon of all.

Climate change represents a major threat to lotic freshwater ecosystems and their ability to support the provision of ecosystem services. England’s chalk streams are in a poor state of health, with significant concerns regarding their resilience, the ability to adapt, under a changing climate. This paper aims to quantify the effect of climate change on hydroecological response, the health of the river, for the River Nar, a SSSI in the south-east of England. To this end, we apply a coupled hydrological and hydroecological modelling framework, with the UKCP09 probabilistic climate projections serving as input (A1B high emissions scenario). Results show that, from 2021 to the end of the century, hydroecological response becomes more heterogeneous. Despite the limited range of the functional feeding groups on the baseline, the River Nar has been able to adapt to extreme events due to inter-annual variation. In the future, this variation is greatly reduced, raising real concerns over the resilience of the river ecosystem under climate change. These new insights into the health of the River Nar, and chalk streams more generally, highlights the necessity of further study and the real need to for changed river management practices.

Plant-based proteins, like those derived from hemp and rapeseed can significantly contribute to a balanced diet and human daily nutritional requirements by providing essential nutrients such as protein, fiber, vitamins, minerals, and antioxidants. According to numerous recent research papers the consumption of plant-based proteins has been associated with numerous health benefits, including reduced risk of chronic diseases such as heart disease, diabetes, and certain cancers. Plant-based diets are often lower in saturated fat and cholesterol and higher in fiber and phytonutrients, which can support overall health and well-being. Present research investigates the nutritional attributes, functional properties, and potential food applications of hemp and rapeseed protein for a potential use in new food product development, with a certain focus on identifying anti-nutritional factors and bioactive compounds. Through comprehensive analysis, anti-nutritional factors and bioactive compounds were elucidated, shedding light on their impact on protein quality and digestibility. The study also delves into the functional properties of hemp and rapeseed protein, unveiling their versatility in various food applications. Insights from this research contribute to a deeper understanding of the nutritional value and functional potential of hemp and rapeseed protein, paving the way for their further utilization in innovative food products with enhanced nutritional value and notable health benefits.

Aim: The main purpose of this feasibility study was to estimate compliance and acceptability rate of a 9-Steps mobile app preparation program. Subsequently, to assess its impact regarding attitudes towards smoking cessation. Methods: The program was only available on versions above iOS 13.3. The ecological study collected user’s data via the app from 04/07/2021 to 28/07/2021. Results: A total of 2331 ecological users completed onboarding; 1926 users (73.9%) completed the step 0 and only 265 users continued in step 1. On average, participants actively engaged with intra-steps activities (85,1%). Most users were highly satisfied at the end of each step. On average 86% found the content of each step comprehensive and relevant. Mann-Whitney tests demonstrated positive changes in attitudes toward smoking cessation on the less confident users: willing (3 to 5, p <.05); confidence (3 to 5, p <.001) and readiness to quit (3 to 5, p <.001). Conclusions: This ecological study corroborates the expected dropout between first use and regular use of health mobile apps. However, engagement and satisfaction rates support the feasibility and acceptability of a 9-step preparation program. Additional research is needed to better understand determinants of regular use of mobile apps and its impact on the target behavior.

The development of plant-based meat analogues has become a significant challenge for the food industry in recent years due to the increasing demand for sustainable and healthier proteins in the context of a global protein transition. Plant-based meat analogues imitate the visual, textural, and chemical properties of traditional meat products and are required to closely resemble meat to appeal to consumers. In addition, consumers demand natural, clean-label, and nutritional and healthy products. To address these challenges, the food industry must develop highly healthy, nutritious, and E-number-free meat analogue products. Understanding the functionality of each ingredient and its role in the food matrix is crucial to being a key player in the innovation of the meat analogues market. This review provides updated information on the primary ingredients utilized for the development of plant-based burger meat alternatives and their functionality. The key components of meat analogue burgers are outlined, including plant proteins, binding agents, fats and oils, flavorings, colorings, preservatives, fortificants, and clean-label considerations.

This study continued the analysis of immunization with the receptor binding domain (RBD) associated with the adjuvants Dimethyldioctadecylammonium bromide (DDA) and Saponin (Sap) (RBD+DDA/Sap) or Aluminum hydroxide (AH) and outer membrane vesicles (OMVs) of Neisseria meningitidis (RBD+AH/OMV); from adult to old age and assessed maternal-fetal transference of antibodies. Outbred Swiss mice were immunized with 2 intramuscular (IM) doses with the antigenic preparations RBD+DDA/Sap, RBD+AH/OMV or RBD alone. The humoral immune response was evaluated using ELISA, avidity-ELISA, Immunoblotting and a Omicron-surrogate-neutralization assay (BA.1), while the cellular immune response was analyzed by ELISpot. Our previous work studied the IgG response; here, we verified that IgM levels were higher right after the immunization doses, but, as IgG, it persisted until 368 days after the immunization. The avidity of IgM increased from low to intermediate-to-high after the booster dose. RBD+DDA/Sap presented neutralizing indexes against Omicron at days 47 and 176, while RBD+AH/OMV showed neutralization on days 21, 47 and 176. Cellular response, measured 465 days after immunization, revealed IFN-Y and IL-4 secretion. The offspring of RBD+DDA/Sap had detectable antibodies, which decreased around 45 days after birth, the last point we analyzed. The results suggested that DDA/Sap is a promising adjuvant mixture to enhance humoral and cellular immune response against SARS-CoV-2, improving maternal-fetal transference of antibodies; cross-reactivity with Omicron variant; and the maintenance of antibodies for a long period.