Biomarkers are objective medical signals and can facilitate the diagnosis and monitoring of a multitude of diseases, including those whose symptomatology is largely subjective.Electrochemical biosensors offer an ideal platform for the application of emerging knowledge resulting from biomarker research. They combine the sensitivity of electrochemical detection techniques with the specificity of a biochemical reaction.In this review article, an introductory reference was made to the usefulness of biomarkers and the importance of their validation, to the problems encountered in the diagnosis of diseases, as well as to the structural characteristics and role of biosensors.ubsequently, applications of electrochemical biosensors for the determination of biomarkers from recent literature were presented, which were classified based on the biological mechanism of recognition of the sensor into enzymatic, immunochemical, DNA, other (biomimetic) and multipotent.