Article
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Microbiomes of Two Pest Fly Species of Pennsylvania Mushroom Houses
Version 1
: Received: 22 May 2024 / Approved: 23 May 2024 / Online: 24 May 2024 (11:53:58 CEST)
How to cite: Sakamoto, J. M.; Shikano, I.; Rasgon, J. L. Microbiomes of Two Pest Fly Species of Pennsylvania Mushroom Houses. Preprints 2024, 2024051593. https://doi.org/10.20944/preprints202405.1593.v1 Sakamoto, J. M.; Shikano, I.; Rasgon, J. L. Microbiomes of Two Pest Fly Species of Pennsylvania Mushroom Houses. Preprints 2024, 2024051593. https://doi.org/10.20944/preprints202405.1593.v1
Abstract
Mushroom cultivation vastly improves the yield of mushrooms under optimized, controlled conditions, but may be susceptible to opportunistic colonization by pest species that can establish themselves, as well as the pathogens and pests they may transmit. Here we describe our investigation into the bacterial communities of adult Lycoriella ingenua (Diptera: Sciaridae) and Megaselia halterata (Diptera: Phoridae) collected from button mushroom (Agaricus bisporus) production houses in Pennsylvania. We collected adult flies and sequenced the hypervariable v4 region of the bacterial 16S rRNA using the Illumina MiSeq. The most abundant bacterial genus detected in both species was Wolbachia, but phylogenetic analysis revealed that the infections are from different clades. Future studies include the characterization of Wolbachia infections on fly behavior and biology, comparison of microbial diversity of fly species colonizing wild mushrooms, and other microbiota that may contribute to the success of certain pest fly species.
Keywords
Mushroom fly; microbiome; Lycoriella ingenua; Megaselia halterata; Wolbachia
Subject
Biology and Life Sciences, Insect Science
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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