UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of Sarcomphalus joazeiro (Mart.) Hauenschild Leaves

José Galberto Martins da Costa; Natália Kelly Gomes De Carvalho; Mariana Pereira da Silva; Débora Odília Duarte Leite; Gerson Javier Gerson Javier; Johnatan Wellisson Da Silva Mendes; Kirley Marques Canuto Kirley Marques Canuto; Paulo Riceli Vasconcelos Ribeiro; Amanda Barros; Ivana Romão; Hélcio Silva

doi:10.20944/preprints202405.0872.v1

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preprints.org > biology and life sciences > biology and biotechnology > doi: 10.20944/preprints202405.0872.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of Sarcomphalus joazeiro (Mart.) Hauenschild Leaves

José Galberto Martins da Costa

^* ,

Natália Kelly Gomes De Carvalho

Mariana Pereira da Silva

Débora Odília Duarte Leite

Gerson Javier Gerson Javier

Johnatan Wellisson Da Silva Mendes

Kirley Marques Canuto Kirley Marques Canuto

Paulo Riceli Vasconcelos Ribeiro

Amanda Barros

Ivana Romão

Hélcio Silva

Version 1 : Received: 13 May 2024 / Approved: 13 May 2024 / Online: 14 May 2024 (05:07:41 CEST)

How to cite: Costa, J. G. M. D.; De Carvalho, N. K. G.; Pereira da Silva, M.; Duarte Leite, D. O.; Gerson Javier, G. J.; Da Silva Mendes, J. W.; Kirley Marques Canuto, K. M. C.; Vasconcelos Ribeiro, P. R.; Barros, A.; Romão, I.; Silva, H. UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of Sarcomphalus joazeiro (Mart.) Hauenschild Leaves. Preprints 2024, 2024050872. https://doi.org/10.20944/preprints202405.0872.v1 Costa, J. G. M. D.; De Carvalho, N. K. G.; Pereira da Silva, M.; Duarte Leite, D. O.; Gerson Javier, G. J.; Da Silva Mendes, J. W.; Kirley Marques Canuto, K. M. C.; Vasconcelos Ribeiro, P. R.; Barros, A.; Romão, I.; Silva, H. UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of Sarcomphalus joazeiro (Mart.) Hauenschild Leaves. Preprints 2024, 2024050872. https://doi.org/10.20944/preprints202405.0872.v1

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MDPI and ACS Style

Costa, J. G. M. D.; De Carvalho, N. K. G.; Pereira da Silva, M.; Duarte Leite, D. O.; Gerson Javier, G. J.; Da Silva Mendes, J. W.; Kirley Marques Canuto, K. M. C.; Vasconcelos Ribeiro, P. R.; Barros, A.; Romão, I.; Silva, H. UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of Sarcomphalus joazeiro (Mart.) Hauenschild Leaves. Preprints 2024, 2024050872. https://doi.org/10.20944/preprints202405.0872.v1

APA Style

Costa, J. G. M. D., De Carvalho, N. K. G., Pereira da Silva, M., Duarte Leite, D. O., Gerson Javier, G. J., Da Silva Mendes, J. W., Kirley Marques Canuto, K. M. C., Vasconcelos Ribeiro, P. R., Barros, A., Romão, I., & Silva, H. (2024). UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of <em>Sarcomphalus joazeiro</em> (Mart.) Hauenschild Leaves. Preprints. https://doi.org/10.20944/preprints202405.0872.v1

Chicago/Turabian Style

Costa, J. G. M. D., Ivana Romão and Hélcio Silva. 2024 "UPLC-PDA-ESI-QDA Characterization and Evaluation of the Antioxidant and Anxiolytic Activities of the Ethanolic Extract of <em>Sarcomphalus joazeiro</em> (Mart.) Hauenschild Leaves" Preprints. https://doi.org/10.20944/preprints202405.0872.v1

Abstract

Anxiety is a multifactorial pathology associated with oxidative stress and changes in different neurotransmitters in the central nervous system (CNS). Sarcomphalus joazeiro (Rhamnaceae) has a predicted effect on the CNS, being considered an alternative for pre-clinical investigations of anxiolytic drugs. The objective of this study is to evaluate the chemical composition, analyze the antioxidant capacity and the anxiolytic effect of the ethanolic extract of S. joazeiro leaves (EEFSJ) in zebrafish. The chemical profile was analyzed by liquid chromatography coupled to mass spectrometry (UPLC-PDA-ESI-QDA). Antioxidant action by DPPH• elimination and ABTS•⁺ capture assays. The 96 h acute toxicity and open field and light and dark tests, for sedative and anxiolytic evaluation, respectively, were applied in vivo, using zebrafish. It was possible to identify 11 compounds from the flavonoid and saponin class. EEFSJ exhibited a mean inhibitory concentration (IC50) of 185.2 ± 2.2 µg/mL and 74.17 ± 1.5 µg/mL against the DPPH• and ABTS•⁺ radicals, respectively. In vivo, EEFSJ demonstrated reduced locomotor activity and an anxiolytic effect similar to diazepam via GABAergic systems, without presenting toxicity within 96 h. These results suggest the safety of using EEFSJ for the development of clinical trials for the production of new anxiolytic drugs.

Keywords

Sarcomphalus joazeiro; antioxidant; anxiolytic; zebrafish

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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