Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Intake of Special Amino Acids Mixture Leads to Blunted Murine Colon Cancer Growth In Vitro and In Vivo

Giovanni Corsetti
* ORCID logo ,
Claudia Romano
,
Silvia Codenotti
,
Lorena Giugno
,
Evasio Pasini
,
Alessandro Fanzani
ORCID logo ,
Tiziano Scarabelli
,
Francesco S Dioguardi
ORCID logo
Version 1 : Received: 25 March 2024 / Approved: 26 March 2024 / Online: 26 March 2024 (07:27:44 CET)

How to cite: Corsetti, G.; Romano, C.; Codenotti, S.; Giugno, L.; Pasini, E.; Fanzani, A.; Scarabelli, T.; Dioguardi, F.S. Intake of Special Amino Acids Mixture Leads to Blunted Murine Colon Cancer Growth In Vitro and In Vivo. Preprints 2024, 2024031528. https://doi.org/10.20944/preprints202403.1528.v1 Corsetti, G.; Romano, C.; Codenotti, S.; Giugno, L.; Pasini, E.; Fanzani, A.; Scarabelli, T.; Dioguardi, F.S. Intake of Special Amino Acids Mixture Leads to Blunted Murine Colon Cancer Growth In Vitro and In Vivo. Preprints 2024, 2024031528. https://doi.org/10.20944/preprints202403.1528.v1

Abstract

Cancer cells require substantial amounts of energy and substrates for their metabolic hyperactivity, enabling the synthesis of new cells at the expense of healthy ones. Preliminary in vitro data suggest that a mix of free essential amino acids (EAA-mix) can promote cancer cell apoptosis by enhancing autophagy. This study aimed to confirm, both in vitro and in vivo, whether EAA intake could influence the development of colon cancer in mice. We investigated changes in cancer proliferation in CT26 cells treated with EAA-mix, and in mice fed with EAA rich modified diets (EAARD) as compared to those on a standard laboratory diet (StD). CT26 cells were injected subcutaneously (s.c.) or intraperitoneally (i.p.). After 21 days, tumors were removed and measured. In vitro data corroborated that EAA-mix impairs cancer growth by inducing autophagy and apoptosis. In vivo data revealed that mice on StD developed significantly larger (s.c.) and more numerous (i.p.) cancers than those on EAARD. EAA administration appears to influence cancer cell survival with notable anti-proliferative properties.

Keywords

cancer; amino acids; colon; nitrogen; diet; mice

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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