Version 1
: Received: 23 March 2024 / Approved: 25 March 2024 / Online: 25 March 2024 (08:20:19 CET)
How to cite:
Mora Martínez, A.L.; Yepes-Pérez, M.; Carrero Contreras, K.A. Production of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) by Bacillus megaterium LVN01 Using Biogas Digestate. Preprints2024, 2024031448. https://doi.org/10.20944/preprints202403.1448.v1
Mora Martínez, A.L.; Yepes-Pérez, M.; Carrero Contreras, K.A. Production of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) by Bacillus megaterium LVN01 Using Biogas Digestate. Preprints 2024, 2024031448. https://doi.org/10.20944/preprints202403.1448.v1
Mora Martínez, A.L.; Yepes-Pérez, M.; Carrero Contreras, K.A. Production of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) by Bacillus megaterium LVN01 Using Biogas Digestate. Preprints2024, 2024031448. https://doi.org/10.20944/preprints202403.1448.v1
APA Style
Mora Martínez, A.L., Yepes-Pérez, M., & Carrero Contreras, K.A. (2024). Production of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) by <em>Bacillus megaterium</em> LVN01 Using Biogas Digestate. Preprints. https://doi.org/10.20944/preprints202403.1448.v1
Chicago/Turabian Style
Mora Martínez, A.L., María Yepes-Pérez and Karent Alexandra Carrero Contreras. 2024 "Production of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) by <em>Bacillus megaterium</em> LVN01 Using Biogas Digestate" Preprints. https://doi.org/10.20944/preprints202403.1448.v1
Abstract
The Bacillus megaterium LVN01 species native to Colombia has demonstrated the ability to metabolize different co-products or industrial waste (such as fique juice, cane molasses, residual glycerol) and accumulate polyhydroxybutyrate (PHB), giving it potential in the bioplastics industry. In this research, the potential of liquid digestate as a carbon source for the production of PHA in fermentation processes with this bacterial strain was evaluated. Favorably, it was found that B. megaterium utilizes the nutrients from this residual substrate to multiply appropriately and efficiently synthesize poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV). Bench scale aerobic batch fermentation, under the operational conditions of this research [Volume: 3L; Temperature: 30.8°C; Agitation: 400 rpm; pH: 7.0 ± 0.2; Disolved Oxygen: 100% saturation; Antifoam 10% (v/v)], generated maximum values of DCW (0.56 g cell L-1) at 60 h, while the maximum PHB yield (360 mg PHB L-1) occurred at 16 h, which is very favorable for sustainable degradable bioplastics production. Additionally, GC-MS and NMR analyses confirmed that the PHBV copolymer synthesized by B. megaterium is made up of the monomers 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV). Furthermore, the thermal properties determined by TGA (Tonset=283.1 ºC, Tendset= 296.98 ºC. Td =290.114 °C) and DSC (Tm= ºC155.7 °C, ΔHf= 19.80 J g-1, Xcr = 18.17%), indicate that it is a thermally stable biopolymer with low percentages of crystallinity, providing flexibility that facilitates molding, adaptation, and application in various industrial sectors.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
