Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Transcriptomic and Metatranscriptomic Analyzes Provide New Insights into the Response of the Pea Aphid Acyrthosiphon pisum (Hemiptera: Aphididae) to Acetamiprid

Zhiyan Cai
ORCID logo ,
Xuhui Zhao
,
Yuxin Qian
,
Kun Zhang
,
Shigang Guo
,
Yunchao Kan
,
Camilo Ayra-Pardo
* ,
Yuqing Wang
* ,
Dandan Li
* ORCID logo
Version 1 : Received: 22 March 2024 / Approved: 22 March 2024 / Online: 22 March 2024 (06:37:42 CET)

A peer-reviewed article of this Preprint also exists.

Cai, Z.; Zhao, X.; Qian, Y.; Zhang, K.; Guo, S.; Kan, Y.; Wang, Y.; Ayra-Pardo, C.; Li, D. Transcriptomic and Metatranscriptomic Analyses Provide New Insights into the Response of the Pea Aphid Acyrthosiphon pisum (Hemiptera: Aphididae) to Acetamiprid. Insects 2024, 15, 274. Cai, Z.; Zhao, X.; Qian, Y.; Zhang, K.; Guo, S.; Kan, Y.; Wang, Y.; Ayra-Pardo, C.; Li, D. Transcriptomic and Metatranscriptomic Analyses Provide New Insights into the Response of the Pea Aphid Acyrthosiphon pisum (Hemiptera: Aphididae) to Acetamiprid. Insects 2024, 15, 274.

Abstract

Acetamiprid is a broad-spectrum neonicotinoid insecticide used in agriculture to control aphids. While recent studies have documented resistance to acetamiprid in several aphid species, the underlying mechanisms are still not fully understood. In this study, we analyzed the transcriptome and metatranscriptome of a laboratory strain of the pea aphid, Acyrthosiphon pisum (Harris, 1776), with reduced susceptibility to acetamiprid after nine generations of exposure to identify candidate genes and the microbiome involved in the adaptation process. Sequencing of the transcriptome of both selected (RS) and non-selected (SS) strains allowed the identification of 14858 genes and 4938 new transcripts. Most of the differentially expressed genes were associated with catalytic activities and metabolic pathways involving carbon and fatty acids. Specifically, the alcohol-forming fatty acyl-CoA reductase (FAR) and acyl-CoA synthetase (ACSF2), both involved in the synthesis of epidermal wax layer components, were significantly upregulated in RS, suggesting that adaptation to acetamiprid involves the synthesis of a thicker protective layer. Metatranscriptomic analysis revealed subtle shifts in the microbiome of RS. These results contribute to a deeper understanding of acetamiprid adaptation by the pea aphid and provide new insights for aphid control strategies.

Keywords

omics; insecticide resistance; symbiotic bacteria; gene expression analysis; insect bioassays

Subject

Biology and Life Sciences, Insect Science

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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