Version 1
: Received: 29 February 2024 / Approved: 1 March 2024 / Online: 1 March 2024 (18:27:35 CET)
Version 2
: Received: 22 March 2024 / Approved: 22 March 2024 / Online: 25 March 2024 (13:34:45 CET)
How to cite:
Aimofumhe, E.S.; Badewole, F.; Smith, D.; Neeland, K.N.; Adetula, O.; Thomas, M.S. Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media. Preprints2024, 2024030071. https://doi.org/10.20944/preprints202403.0071.v2
Aimofumhe, E.S.; Badewole, F.; Smith, D.; Neeland, K.N.; Adetula, O.; Thomas, M.S. Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media. Preprints 2024, 2024030071. https://doi.org/10.20944/preprints202403.0071.v2
Aimofumhe, E.S.; Badewole, F.; Smith, D.; Neeland, K.N.; Adetula, O.; Thomas, M.S. Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media. Preprints2024, 2024030071. https://doi.org/10.20944/preprints202403.0071.v2
APA Style
Aimofumhe, E.S., Badewole, F., Smith, D., Neeland, K.N., Adetula, O., & Thomas, M.S. (2024). Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media. Preprints. https://doi.org/10.20944/preprints202403.0071.v2
Chicago/Turabian Style
Aimofumhe, E.S., Ololade Adetula and Marlon Sheldon Thomas. 2024 "Evaluation of the Ability of Using Shear-horizontal Surface Acoustic Wave Biosensors to Perform Sterility Testing of Nutrient Media" Preprints. https://doi.org/10.20944/preprints202403.0071.v2
Abstract
Abstracts
In this article, we describe a simple procedure for evaluating the sterility of nutrient media using a shear-horizontal surface acoustic wave (SH-SAW) biosensor. We used the SH-SAW biosensor platform to monitor the time-dependent phase responses of the material for the media that binds to a monolayer of 3-mercaptopropanyl-N-hydroxy succinimide on the biosensor surface. Increases in the phase shift less than 50% of the starting phase shift recorded 180 minutes after the sterilization indicate a successful sterility test of the media. Our devices were then applied to sterility testing using a viable Escherichia coli (E. coli) K-12 solution to confirm our hypothesis. Filtered LB Broth was the negative control, and a 24-hour culture of E. coli was the positive control. Before testing, the cell solution was pelleted, the media decanted, and then replaced with a 1x phosphate-buffered saline solution (1x PBS). Since we know that the N-hydroxysuccinimide residues will bind to amine groups, only in cases where microbial species are present will the surface binding increase greater than 50%. We confirmed our hypothesis using SH-SAW biosensors and overnight E. coli cultures. The sensitivity for our 250 MHz quartz SH-SAW sensors was calculated based on the response from the E. coli K-12 solution over the linear dynamic range, which appears to be 2x10^4 – 2x10^7 CFU per ml and has a sensitivity of S = 1.655x10^5 o m2 Kg-1. The limit of detection (LOD) for these sensors was calculated to be approximately 2x10^-9 grams (g), which is estimated to be approximately 2,000 Escherichia coli (E. coli) K-12 cells. However, the sensitivity and insertion loss remained approximately unchanged before and after coating the sensor with 3,3'-Dithiobis(succinimidyl) Propionate (DSP).
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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