Version 1
: Received: 2 February 2024 / Approved: 6 February 2024 / Online: 6 February 2024 (09:24:04 CET)
How to cite:
Entrenas-García, C.; Argüello, H.; Calderón-Santiago, M.; Priego-Capote, F.; Zaldívar-López, S.; Garrido, J.J. Salmonella typhimurium Alteration of Small Intestine Metabolism Early after Infection Is Linked to Microbiome Changes. Preprints2024, 2024020353. https://doi.org/10.20944/preprints202402.0353.v1
Entrenas-García, C.; Argüello, H.; Calderón-Santiago, M.; Priego-Capote, F.; Zaldívar-López, S.; Garrido, J.J. Salmonella typhimurium Alteration of Small Intestine Metabolism Early after Infection Is Linked to Microbiome Changes. Preprints 2024, 2024020353. https://doi.org/10.20944/preprints202402.0353.v1
Entrenas-García, C.; Argüello, H.; Calderón-Santiago, M.; Priego-Capote, F.; Zaldívar-López, S.; Garrido, J.J. Salmonella typhimurium Alteration of Small Intestine Metabolism Early after Infection Is Linked to Microbiome Changes. Preprints2024, 2024020353. https://doi.org/10.20944/preprints202402.0353.v1
APA Style
Entrenas-García, C., Argüello, H., Calderón-Santiago, M., Priego-Capote, F., Zaldívar-López, S., & Garrido, J.J. (2024). <em>Salmonella typhimurium </em>Alteration of Small Intestine Metabolism Early after Infection Is Linked to Microbiome Changes. Preprints. https://doi.org/10.20944/preprints202402.0353.v1
Chicago/Turabian Style
Entrenas-García, C., Sara Zaldívar-López and Juan J. Garrido. 2024 "<em>Salmonella typhimurium </em>Alteration of Small Intestine Metabolism Early after Infection Is Linked to Microbiome Changes" Preprints. https://doi.org/10.20944/preprints202402.0353.v1
Abstract
Salmonella Typhimurium infection in pigs is characterized by an intense inflammatory response in early infection which seems to dysregulate host physiology including the gut microbiota. Both, changes in host physiology and microbiome composition, must entail modifications within the gut environment. This study analyzes the gut metabolome in early infected pigs (48 hours after infection) and non-infected controls by untargeted metabolomics (LC–QTOF MS/MS and GC–TOF MS), supported with microbiome data (16S rRNA abundance and prediction functional analyses). The metabolomic profile in Salmonella-infected pigs differed from healthy controls in 39 of these metabolites, including eight amino acids, four bile acids and carboxylic acids, three carnitines and sugar derivates, eleven fatty acids and six other compounds. Changes in amino acids abundance and the observed decrease of proteolytic bacteria such as Lactobacillus or Bacteroides could favor the niche colonization by Desulfovibrionaceae. In addition, the decrease in short chain fatty acids and tricarboxylic acids cycle was correlated to a decrease in beneficial bacteria in infected pigs. Interestingly, we observed an increase of bile acids concentration and compounds such as succinic acid or pantothenic acid, which may boost the inflammatory response. Altogether, the results reveal how S. Typhimurium infection alters the gut microbiome composition and prompts changes in the metabolism with a potential interaction between intestinal metabolism and microbiota abundance.
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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