Expression Profiling Uncovers Novel Genes Associated with Plant Hormone Signal Transduction Involved in Regulation of Anthocyanin Biosynthesis in Red-Fleshed Apple Cultivars

Sylwia Keller-Przybylkowicz; Michal Oskiera; Xueqing Liu; Laiquing Song; Lingling Zhao; Xiaoyun Du; Dorota E. Kruczyńska; Agnieszka Walencik; Norbert Jakub Kowara; Grzegorz Bartoszewski

doi:10.20944/preprints202401.0732.v1

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preprints.org > biology and life sciences > biology and biotechnology > doi: 10.20944/preprints202401.0732.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Expression Profiling Uncovers Novel Genes Associated with Plant Hormone Signal Transduction Involved in Regulation of Anthocyanin Biosynthesis in Red-Fleshed Apple Cultivars

Sylwia Keller-Przybylkowicz

^* ,

Grzegorz Bartoszewski

Version 1 : Received: 9 January 2024 / Approved: 9 January 2024 / Online: 10 January 2024 (04:33:22 CET)

A peer-reviewed article of this Preprint also exists.

Keller-Przybylkowicz, S.; Oskiera, M.; Liu, X.; Song, L.; Zhao, L.; Du, X.; Kruczynska, D.; Walencik, A.; Kowara, N.; Bartoszewski, G. Transcriptome Analysis of White- and Red-Fleshed Apple Fruits Uncovered Novel Genes Related to the Regulation of Anthocyanin Biosynthesis. Int. J. Mol. Sci. 2024, 25, 1778. Keller-Przybylkowicz, S.; Oskiera, M.; Liu, X.; Song, L.; Zhao, L.; Du, X.; Kruczynska, D.; Walencik, A.; Kowara, N.; Bartoszewski, G. Transcriptome Analysis of White- and Red-Fleshed Apple Fruits Uncovered Novel Genes Related to the Regulation of Anthocyanin Biosynthesis. Int. J. Mol. Sci. 2024, 25, 1778.

Abstract

Apple red flesh coloration is a result of the performance of biochemical pathway involved in the biosynthesis of anthocyanins and anthocyanidins. Based on apple genome analysis, high number of regulatory genes, mainly transcription factors such as MYB, which are components of regulatory complex MYB-bHLH-WD40, and several structural genes (PAL, 4CL, CHS, CHI, F3H, DFR, ANS, UFGT) involved in anthocyanin biosynthesis have been identified so far. In this study, we investigated previously unrecognized genes involved in the constitution of red-flesh apple phenotype. These genes could be concerned as molecular markers for early selection of new apple cultivars. Based on comparative transcriptome analysis of apples with different fruit flesh coloration, we successfully identified and characterized ten genes from plant hormone transduction pathway of: auxin (GH3); cytokinins (B-ARR); gibberellins (DELLA); abscisic acid (SnRK2 and ABF); brassinosteroids (BRI1, BZR1 and TCH4); jasmonic acid (MYC2); and salicylic acid (NPR1). In presented studies an analysis of their expression profiles was performed in immature and ripe fruits of red-fleshed cultivars. Generally, we have uncovered, the set of the novel genes, mediating regulation of the abscisic acid, salicylic acid, cytokinins and jasmonic acid signaling and preliminarily described their role in anthocyanin biosynthesis, accumulation and degradation. Their expression was not characterized in apple fruits so far. Presented study, allowed to underline the relationship of genes from hormone signal transduction pathway with UFGT gene, directly responsible for anthocyanin color transformation as well as confirmed their crucial role of plant hormone regulation and anthocyanin accumulation during apple fruit ripening.

Keywords

apple; expression profiling; KEGG; red-flesh fruits; RNA-seq; RT-qPCR; transcriptome

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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