Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Phytoene Desaturase (Pds) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.)

Fernanda dos Santos Nascimento
ORCID logo ,
Marcelly Santana Mascarenhas
ORCID logo ,
Samantha Costa Boaventura
,
Carla Catharina Hora Souza
,
Andresa Priscila de Souza Ramos
,
Anelita de Jesus Rocha
ORCID logo ,
Julianna Matos da Silva Soares
ORCID logo ,
Leandro Eugenio Cardamone Diniz
ORCID logo ,
Tiago Antônio De Oliveira Mendes
,
Claudia Fortes Ferreira
ORCID logo ,
Edson Perito Amorim
* ORCID logo
Version 1 : Received: 8 January 2024 / Approved: 8 January 2024 / Online: 8 January 2024 (11:27:51 CET)

How to cite: Nascimento, F.D.S.; Mascarenhas, M.S.; Boaventura, S.C.; Souza, C.C.H.; Ramos, A.P.D.S.; Rocha, A.D.J.; Soares, J.M.D.S.; Diniz, L.E.C.; De Oliveira Mendes, T.A.; Ferreira, C.F.; Amorim, E.P. Phytoene Desaturase (Pds) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.). Preprints 2024, 2024010588. https://doi.org/10.20944/preprints202401.0588.v1 Nascimento, F.D.S.; Mascarenhas, M.S.; Boaventura, S.C.; Souza, C.C.H.; Ramos, A.P.D.S.; Rocha, A.D.J.; Soares, J.M.D.S.; Diniz, L.E.C.; De Oliveira Mendes, T.A.; Ferreira, C.F.; Amorim, E.P. Phytoene Desaturase (Pds) Gene-Derived Markers Identify “A” and “B” Genomes in Banana (Musa spp.). Preprints 2024, 2024010588. https://doi.org/10.20944/preprints202401.0588.v1

Abstract

Phytoene desaturase (PDS) is a plant enzyme involved in carotenoid biosynthesis. The PDS gene has been used as a selective marker for genome editing in several plant species, including banana (Musa spp.). Its knockout promotes dwarfism and albinism, characteristics that are easily recognizable and highly favorable. In Musa spp., the A genome increases fruit production and quality, whereas the B genome is associated with tolerance to biotic and abiotic stresses. The objective of this study was to identify a molecular marker in the PDS gene to easily discriminate the A and B genomes of banana. A 2166 bp fragment for the ‘PDSMa’ marker was identified as polymorphic for the A genome (identification accuracy of 99.33%), whereas ~332 and ~225 bp fragments were detected for the ‘PDSMb’ primer with 100% accuracy using MedCalc software. To the best of our knowledge, this is the first study to use the PDS gene to determine doses of the A genome and identify the B genome in Musa spp., which will aid in evaluating the genomic constitution of banana hybrids and accessions at the seedling stage, and accelerating their classification in crop genetic improvement programs.

Keywords

Gene composition; phytoene desaturase; molecular marker; genetic improvement; Musa spp.

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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