Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genome-wide Identification of Universal Stress Protein Family Genes and Expression in Mechanical damage and DON stress Response of Potato Based on the Transcriptome

Tianshuai Qi
ORCID logo ,
Fumeng He
,
Xinqi Zhang
,
Jiaqi Wang
,
Zengli Zhang
,
Heran Jiang
,
Biao Zhao
,
Chong Du
,
Yunzhu Che
,
Xu Feng
,
Yingnan Wang
* ,
Fenglan Li
*
Version 1 : Received: 19 December 2023 / Approved: 19 December 2023 / Online: 19 December 2023 (11:31:33 CET)

A peer-reviewed article of this Preprint also exists.

Qi, T.; He, F.; Zhang, X.; Wang, J.; Zhang, Z.; Jiang, H.; Zhao, B.; Du, C.; Che, Y.; Feng, X.; Wang, Y.; Li, F. Genome-Wide Identification and Expression Profiling of Potato (Solanum tuberosum L.) Universal Stress Proteins Reveal Essential Roles in Mechanical Damage and Deoxynivalenol Stress. Int. J. Mol. Sci. 2024, 25, 1341. Qi, T.; He, F.; Zhang, X.; Wang, J.; Zhang, Z.; Jiang, H.; Zhao, B.; Du, C.; Che, Y.; Feng, X.; Wang, Y.; Li, F. Genome-Wide Identification and Expression Profiling of Potato (Solanum tuberosum L.) Universal Stress Proteins Reveal Essential Roles in Mechanical Damage and Deoxynivalenol Stress. Int. J. Mol. Sci. 2024, 25, 1341.

Abstract

Universal Stress Protein (USP) plays an important regulatory role in the response to abiotic stress. Most of the research related to USP so far has been conducted on plant models such as Arabidopsis, rice, and cotton. The potato is one of the four major food crops in the world. Therefore, it is of great significance to study the expression pattern of the potato StUSP gene family under abiotic stress conditions. In this study, a total of 108 USP genes were identified from the genome of the Atlantic potato, divided into four subgroups. And based on the genetic structure, the physical and chemical properties of proteins and other aspects of their biological characteristics are comprehensively analyzed. Collinear analysis showed that the homologous genes of StUSPs and four other representative species (Arabidopsis, rice, coyote tobacco, and tomato) were highly conserved. The cis-regulatory elements of the StUSPs promoter are involved in plant hormones, environmental stress, mechanical damage, and light response. RNA-seq analysis showed that under different abiotic stresses, there are differences in the expression patterns of members of each subgroup. WGCNA co-expression analysis of the central gene showed that the differential co-expression gene is mainly involved in the plant-pathogen response process, plant hormone signal transduction, and the biosynthesis process of secondary metabolites. Through qRT-PCR analysis, it was confirmed that StUSP13, StUSP14, StUSP15, and StUSP41 may be important candidate genes involved in the response to adversity stress in potatoes. The results of this study provide the basis for further research on the functional analysis of StUSPs in the potato response to adversity stress.

Keywords

Potato; USP gene family; abiotic stress; bioinformatics analysis; transcriptome

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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