Qiao, Z.; Li, J.; Zhang, X.; Guo, H.; He, C.; Zong, D. Genome-Wide Identification, Expression Analysis, and Subcellular Localization of DET2 Gene Family in Populus yunnanensis. Genes2024, 15, 148.
Qiao, Z.; Li, J.; Zhang, X.; Guo, H.; He, C.; Zong, D. Genome-Wide Identification, Expression Analysis, and Subcellular Localization of DET2 Gene Family in Populus yunnanensis. Genes 2024, 15, 148.
Qiao, Z.; Li, J.; Zhang, X.; Guo, H.; He, C.; Zong, D. Genome-Wide Identification, Expression Analysis, and Subcellular Localization of DET2 Gene Family in Populus yunnanensis. Genes2024, 15, 148.
Qiao, Z.; Li, J.; Zhang, X.; Guo, H.; He, C.; Zong, D. Genome-Wide Identification, Expression Analysis, and Subcellular Localization of DET2 Gene Family in Populus yunnanensis. Genes 2024, 15, 148.
Abstract
(1) Background: Brassinosteroids (BRs) are an important hormone that participates in almost all stages of plant growth and development, and sterol dehydrogenase is a key enzyme that is involved in the biosynthesis of BRs. However, the steroid dehydrogenase gene family in Populus yunnanensis Dode (P. yunnanensis) has not been studied; (2) Methods: The PyDET2 gene family was identified and bioinformatics analyzed. Three genes were screened based on the RNA-seq of stem tip, the PyDET2e was further investigated by RT-qPCR (real-time quantitative polymerase chain reaction) and subcellular localization; (3) Results: We identified 14DET2 family genes in P. yunnanensis that were classified into 4 groups according to sequence similarity and phylogenetic relationship, and 10 conserved protein motifs had been identified. The gene structure, chromosome distribution, collinearity, and codon preference of all PyDET2 genes identified in the genome of P. yunnanensis were analyzed. The codon of this family is biased to the end of A/U, and natural selection has a great influence on it. The expression level of PyDET2e in September was higher than in July, and it was significantly expressed in stems, stem tips, and leaves. The PyDET2e protein was localized in chloroplasts; (4) Conclusions: The PyDET2e is involved in the development of P. yunnanensis in the rapid growth stage. The systematic analysis provided a basis for the genome-wide identification of genes related to brassinolide biosynthesis processes in P. yunnanensis, and laid a foundation for the study on the mechanism of rapid growth of P. yunnanensis.
Keywords
Populus yunnanensis; DET2; subcellular localization; brassinolides biosynthesis; rapid growth
Subject
Biology and Life Sciences, Forestry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
