Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Involvement of Abscisic Acid in Transition of Pisum sativum L. from Germination to Post-germination Stage

Galina Smolikova
* ORCID logo ,
Ekaterina Krylova
,
Ivan Petřík
,
Polina Vilis
,
Aleksander Vikhorev
,
Ksenia Strygina
,
Miroslav Strnad
,
Andrej Frolov
,
Elena Khlestkina
ORCID logo ,
Sergei Medvedev
ORCID logo
Version 1 : Received: 8 December 2023 / Approved: 12 December 2023 / Online: 12 December 2023 (11:09:39 CET)

A peer-reviewed article of this Preprint also exists.

Smolikova, G.; Krylova, E.; Petřík, I.; Vilis, P.; Vikhorev, A.; Strygina, K.; Strnad, M.; Frolov, A.; Khlestkina, E.; Medvedev, S. Involvement of Abscisic Acid in Transition of Pea (Pisum sativum L.) Seeds from Germination to Post-Germination Stages. Plants 2024, 13, 206. Smolikova, G.; Krylova, E.; Petřík, I.; Vilis, P.; Vikhorev, A.; Strygina, K.; Strnad, M.; Frolov, A.; Khlestkina, E.; Medvedev, S. Involvement of Abscisic Acid in Transition of Pea (Pisum sativum L.) Seeds from Germination to Post-Germination Stages. Plants 2024, 13, 206.

Abstract

Transitions from seed to seedling represent a critical developmental step in the life cycle of higher plants, dramatically affecting plant ontogenesis and stress tolerance. The release from dormancy to acquiring the germination ability is defined by a balance of phytohormones, with a substantial contribution of abscisic acid (ABA), which inhibits germination. We studied the embryonic axes of Pisum sativum L. before and after radicle protrusion. Our previous work compared RNA sequencing-based transcriptomics in the embryonic axes isolated before and after radicle protrusion. The current study aims to analyze ABA-dependent gene regulation during the transition of embryonic axes from germination to post-germination stage. First, we determined the levels of abscisates (ABA, phaseic acid, dihydrophaseic acid, and neo-phaseic acid) using the ultra-high-performance liquid chromatography-tandem mass spectrometry. Second, we made a detailed annotation of ABA-associated genes using RNA sequencing-based transcriptome profiling. Finally, we analyzed the DNA methylation pattern in the promoters of the PsABI3, PsABI4, and PsABI5 genes. We showed that changes in the abscisate profile are characterized by the accumulation of ABA catabolites, and the ABA-related gene profile is accompanied by the upregulation of genes controlling the seedling development and the downregulation of genes controlling the water deprivation. The expression of ABI3, ABI4 and ABI5, which encode the crucial transcription factors during late maturation, was downregulated by more than 20-fold, and their promoters exhibited a high level of methylation already at the late germination stage. Thus, although ABA remains important, other regulators seems to be involved in the transition from seed to seedling.

Keywords

abscisic acid, DNA methylation, embryonic axes, ABA-associated genes, Pisum sativum L., seed-to-seedling transition

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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