Yurkov, A.P.; Kryukov, A.A.; Gorbunova, A.O.; Kudriashova, T.R.; Kovalchuk, A.I.; Gorenkova, A.I.; Bogdanova, E.M.; Laktionov, Y.V.; Zhurbenko, P.M.; Mikhaylova, Y.V.; Puzanskiy, R.K.; Bagrova, T.N.; Yakhin, O.I.; Rodionov, A.V.; Shishova, M.F. Diversity of Arbuscular Mycorrhizal Fungi in Distinct Ecosystems of the North Caucasus, a Temperate Biodiversity Hotspot. J. Fungi2024, 10, 11.
Yurkov, A.P.; Kryukov, A.A.; Gorbunova, A.O.; Kudriashova, T.R.; Kovalchuk, A.I.; Gorenkova, A.I.; Bogdanova, E.M.; Laktionov, Y.V.; Zhurbenko, P.M.; Mikhaylova, Y.V.; Puzanskiy, R.K.; Bagrova, T.N.; Yakhin, O.I.; Rodionov, A.V.; Shishova, M.F. Diversity of Arbuscular Mycorrhizal Fungi in Distinct Ecosystems of the North Caucasus, a Temperate Biodiversity Hotspot. J. Fungi 2024, 10, 11.
Yurkov, A.P.; Kryukov, A.A.; Gorbunova, A.O.; Kudriashova, T.R.; Kovalchuk, A.I.; Gorenkova, A.I.; Bogdanova, E.M.; Laktionov, Y.V.; Zhurbenko, P.M.; Mikhaylova, Y.V.; Puzanskiy, R.K.; Bagrova, T.N.; Yakhin, O.I.; Rodionov, A.V.; Shishova, M.F. Diversity of Arbuscular Mycorrhizal Fungi in Distinct Ecosystems of the North Caucasus, a Temperate Biodiversity Hotspot. J. Fungi2024, 10, 11.
Yurkov, A.P.; Kryukov, A.A.; Gorbunova, A.O.; Kudriashova, T.R.; Kovalchuk, A.I.; Gorenkova, A.I.; Bogdanova, E.M.; Laktionov, Y.V.; Zhurbenko, P.M.; Mikhaylova, Y.V.; Puzanskiy, R.K.; Bagrova, T.N.; Yakhin, O.I.; Rodionov, A.V.; Shishova, M.F. Diversity of Arbuscular Mycorrhizal Fungi in Distinct Ecosystems of the North Caucasus, a Temperate Biodiversity Hotspot. J. Fungi 2024, 10, 11.
Abstract
Background: Number of investigations, which are focused on arbuscular mycorrhiza fungi (AMF) biodiversity is still limited. The analysis of AMF taxa in the North Caucasus, a temperate biodiversity hotspot, used to be limited by the genera level. The aim of this study was to define AMF biodiversity at the species level in the North Caucasus biotopes.
Methods: Molecular genetic identification of fungi was carried out with ITS1 and ITS2 regions as barcodes, sequencing by Illumina MiSeq, analysis of phylogenetic trees for individual genera, searching the operational taxonomic units (OTUs) with identification at the species level. Sequences from MaarjAM and NCBI GenBank were used as references.
Results: We analyzed >10 million reads in soil samples for 3 biotopes to estimate fungal biodiversity. 50 AMF species from 20 genera were found. The valley biotopes (disturbed ecosystems, grasslands) were characterized by a greater AMF diversity, as well as the number of OTUs and species, in comparison with the forest biotopes.
Conclusion: Our hypothesis is that high AMF biodiversity for the river valley can be associated with a higher percentage of annual plants in these biotopes, as well as with the active development of restorative successional processes.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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